• Asian-Australasian Journal of Animal Sciences
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• v.14 no.12
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• pp.1769-1774
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• 2001

Bacillus licheniformis strains L-25 and PWD-1 are two thermophilic feather-degrading bacteria. Despite isolated from different environmental conditions, they were both capable of breaking down chicken feathers and growing in a medium in which feather was the only source of carbon and nitrogen. A 1.46-kb keratinase gene (ker B) was isolated from strain L-25 by a polymerase chain reaction (PCR) using L-25 genomic DNA as templates. Sequencing results reveal that ker B shares great sequence identity with a previously published keratinase gene of B. licheniformis PWD-1 (ker A). Only two amino acids differences were found in the deduced amino acid sequence between the keratinases from L-25 and PWD-1. However several nucleotide changes were found upstream of the putative promoter region. Protease inhibition studies indicated that neutral protease activity accounted for approximate 25 to 30% of total extracellular proteolytic activity produced by strain L-25 in the feather medium. In contrast, no measurable neutral protease activity was produced by strain PWD-1 in the feather medium. When glucose (1%), a common catabolic repressor, was added into the feather medium, L-25 was still able to grow and produce keratinase. Strain PWD-1 produced no neutral protease activity and its growth was severely inhibited in the feather medium containing glucose. L-25 produced an enhanced level of keratinase in the feather medium in comparison with PWD-1.

• Proceedings of the Korean Society of Food Hygiene and Safety Conference
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• 2001.10a
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• pp.28-31
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• 2001

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.308.1-308.1
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• 2008

• Safety and Health at Work
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• v.7 no.3
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• pp.208-212
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• 2016

Background: Outdoor workers are at risk of high ultraviolet radiation exposure, and may have difficulty using sun protection. The objectives were to determine the prevalence of sun protection behaviors in a sample of outdoor construction workers, and to assess which factors predict better sun protection practices. Methods: Participants were recruited via construction unions. Workers answered a questionnaire on demographics, skin cancer risk, sun protection behaviors, and job. Sun protection behavior scores (from questions on sunscreen use, sleeved shirt, hat, shade seeking, sunglasses) were calculated by converting Likert-scale answers to scores from 0 to 4, and taking the mean (separately for work and leisure). Determinants of sun protection behavior scores were examined for work and leisure using generalized linear models. Results: Seventy-seven workers had complete questionnaire data (participation 98%). Sun protection behaviors used most often were hats (79% often/always) and sleeved shirts (82% often/always); least prevalent were shade-seeking (8% often/always) and sunscreen (29% often/always). For both work and leisure scores, the strongest predictor was skin type, with fairer-skinned individuals having higher sun protection behavior scores. Workers had higher scores at work than on weekends. Workplaces that required hats and sleeved shirts for safety purposes had higher protection behavior scores. Conclusion: This high-participation rate cohort helps characterize sun protection behaviors among outdoor workers. Workers practiced better sun protection at work than on weekends, suggesting that workplace policies supportive of sun protection could be useful for skin cancer prevention in the construction industry.

• Safety and Health at Work
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• v.2 no.3
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• pp.273-281
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• 2011

Objectives: Studies examining healthcare workers' exposure to antineoplastic drugs have focused on the drug preparation or drug administration areas. However, such an approach has probably underestimated the overall exposure risk as the drugs need to be delivered to the facility, transported internally and then disposed. The objective of this study is to determine whether drug contamination occurs throughout a facility and, simultaneously, to identify those job categories that are potentially exposed. Methods: This was a multi-site study based in Vancouver, British Columbia. Interviews were conducted to determine the departments where the drugs travel. Subsequent site observations were performed to ascertain those surfaces which frequently came into contact with antineoplastic drugs and to determine the job categories which are likely to contact these surfaces. Wipe samples were collected to quantify surface contamination. Results: Surface contamination was found in all six stages of the hospital medication system. Job categories consistently found to be at risk of exposure were nurses, pharmacists, pharmacy technicians, and pharmacy receivers. Up to 11 job categories per site may be at risk of exposure at some point during the hospital medication system. Conclusion: We found drug contamination on select surfaces at every stage of the medication system, which indicates the existence of an exposure potential throughout the facility. Our results suggest that a broader range of workers are potentially exposed than has been previously examined. These results will allow us to develop a more inclusive exposure assessment encompassing all healthcare workers that are at risk throughout the hospital medication system.

• Proceedings of the Ginseng society Conference
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• 1990.06a
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• pp.155-167
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• 1990

Ginseng Is renowned for both its medicinal and herbal uses and successful cultivation of Panax ginseng in Asia and Panax quinquefolium in North America has until recently taken place in the native geographical ranges of the plants. As a consequence of the potential high capital return and anticipated increases in consumer consumption, commercial cultivation of American ginseng now occurs well outside the native range of the plant in North America. In fact, the region of greatest expansion of cultivation is in the semi-arid interior region of British Columbia, Canada. Linked with this expansion is the potential domination of the ginseng industry by agricultural corporations. In the interior of British Columbia, the native deciduous forest environment of eastern North America is simulated with elevated polypropylene shade and a surface covering of straw mulch. The architecture of these environments is designed to permit maximum machinery usage and to minimize labor requirements. Further, with only a four- years growth cycle, plant densities in the gardens are high. In this hot, semi-arid environment, producers believe they have a competitive advantage over other regions in North America because of the low precipitation rates. This helps to minimize atmospheric humidity such that the conditions for fungal disease development are reduced. If soil moisture level become limited, supplemental water can be provided by irrigation. The nature of the radiation and energy balance regimes of the shade and many environments promotes high soil moisture levels. Also, the modified environment redlines soil heating. This can result in an aerial environment for the plant that is stressful and a rooting zone environment that is suloptimal. The challenge of further refining the man modified environment for enhanced plant growth and health still remains. Keywords Panax ginseng, Panax quinquefolium, cultivation, ginseng production.

• Journal of Ginseng Research
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• v.14 no.2
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• pp.297-309
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• 1990

Ginseng is renowned for both its medicinal and herbal uses and successful cultivation of Panax ginseng in Asia and Panax Vtiinvtiefolilim in North America has until recently taken place in the native geographical ranges of the plants. As a consequence of the potential high capital return and anticipated increases in consumer consumption, commercial cultivation of American ginseng now occurs well outside the native range of the plant in North America. In fact, the region of greatest expansion of cultivation is in the semi-arid interior region of British Columbia, Canada. Linked with this expansion is the potential domination of the ginseng industry by agricultural corporations. In the interior of British Columbia, the native decidous forest environment of eastern North America is simulated with elevated polypropylene shade and a sllrface covering of straw mulch. The architecture of these environments is designed to permit maximillm machinery useage and to minimize labour requirements. Further, with only a four-year growth cycle, plant densities in the gardens are high. In this hot, semiarid environment, producers believe they have a competitive advantage over other regions in North America because of the low precipitation rates. This helps to minimize atmospheric humidity such that the conditions for fungal disease development are reduced. If soil moisture levels become limited, supplemental water can be provided by irrigation. The nature of the radiation and energy balance regimes of the shade and much environment promotes high soil moistilre levels. Also, the modified environment reduces soil heating. This can result in an aerial environment for the plant that is stressful and a rooting zone environment that is sub-optimal. The challenge of further refining the man modified environment for enhanced plant growth and health still remains.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2004.05a
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• pp.237-238
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• 2004

• Proceedings of the Korean Information Science Society Conference
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• 2010.11a
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• pp.1-2
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• 2010

• Proceedings of the Zoological Society Korea Conference
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• 2001.10a
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• pp.214.2-215
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• 2001

No Abstract, See Full Text