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Application Methods for Enhancing Phytotoxicity of Glyphosate I. Effects of Surfactants and Spray Volumes on Leaf Retention of Various Plant Species (Glyphosate의 살초효과(殺草效果) 증진(增進)을 위한 살포방법(撒布方法)에 관(關)한 연구(硏究) I. 계면활성제(界面活性劑)와 살포량(撒布量)에 따른 초종별(草種別) Glyphosate 엽면부착량(葉面附着量)의 차이(差異))

  • Lee, J.J.;Pyon, J.Y.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.58-63
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    • 1987
  • Laboratory and greenhouse studies were conducted to evaluate effects of spray volumes and surfactants, L-77 and Triton CS-7 on leaf retention of glyphosate in Zea mays, Hordeum vulgare, Artemisia princeps, Trifolium repens and Equisetum arvense. Leaf retention was increased when glyphosate with L-77 or Triton CS-7 was applied in a spray volumes of 10-20 l/10a in Zea mays and 40 l/10a in Artemisia princeps but decreased when spray volumes were higher than above volumes and 40-80 l/10a in Trifolium repens. Hordeum vulgare leaves retained more spray when glyphosate with L-77 or Triton CS-7 was applied in all spray volumes tested. Equisetum arvense retained higher amount of spray by application of glyphosate with L-77 compared with Triton CS-7 and no surfactant treatments.

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Soil Microbial Community Analysis in Large Patch (Rhizoctonia solani AG2-2 IV) (갈색퍼짐병 발병토양의 미생물 군집 분석)

  • Lee, Jung Han;Min, Gyu Young;Shim, Gyu Yul;Jeon, Chang Wook;Choi, Su min;Han, Jeong Ji;Kwak, Youn-Sig
    • Weed & Turfgrass Science
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    • v.4 no.2
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    • pp.124-128
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    • 2015
  • Large patch, caused by Rhizoctonia solani AG2-2 IV, is a soil-born disease that is the most important of warm season turfgrass such as zoysia and Bermuda grass. This study was conducted to analysis of the soil microbial community structure on large patch. Center of the large patch (CLC), edge (CLE) and healthy (CLH) part of microbial communities were examined using metagenomics in Phylum level. Distribution trends of the rhizosphere microorganisms were similar to the order Proteobacteria, Acidobacteria, Chloroflexi, Firmicutes, Planctomycetes, Gemmatimonadetes, Nitrospira, Cyanobactria and Verrucomicrobia in soil collections. Contrastively Actinobacteria was more 56% abundant in healthy part soil (16%) than in the center (9.28%) or edge (10.84%) parts. Taxonomic distributions were compared among the CLC, CLE and CLH, total 6,948 OTUs were detected in the CLC, 6,505 OTUs for the CLE and 5,537 OTUs were detected in the CLE. Distributions of Actinobacteria OTUs were appeared 615 OTUs in the CLC, 709 OTUs in the CLE and 891 OTUs in the CLH. Among Actinobacteria, 382 OTUs were overlapped in the all soils. Not matched OTUs of CLH (286 OTUs) was detected 23 times higher than CLC (91 OTUs) and CLE (126 OTUs).

Biological Control of Large Patch Disease by Streptomyces spp. in Turfgrass (스트렙토마이세스를 이용한 잔디 갈색퍼짐병의 생물적 방제)

  • Jeon, Chang Wook;Lee, Jung Han;Min, Gyu Young;Kwak, Youn-Sig
    • Weed & Turfgrass Science
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    • v.5 no.1
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    • pp.29-34
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    • 2016
  • Large patch disease in Zoysia japonica Steud. is the most destructive disease in turfgrass. For large patch management, it has been dependent on chemical controls but pesticides are harmful to soil, water and biodiversity. In this study, we evaluated 4 Streptomyces spp. strains (S2, S5, S8 and S12) which were selected in previous studies using metagenome approaches. Root colonization of the strains, large patch suppressing effect and the pathogen density change in actual golf course were investigated to evaluate biological control potential of the strains. All strains exhibited reliable root colonization ability that strains populations were higher than $6log\;cfu\;g^{-1}$ in turfgrass rhizosphere. The pathogen density, with S8 treatment, was detected average of 0.7 after a week and average of 1.2 after 4 weeks. Disease control and suppressive the pathogen population by S8 strain showed higher efficiency than other strains. S8 was applied in an actual golf course for the large patch control and pathogen density. The pathogen density in S8 treatment plot was detected below 1.6 per toothpick and lower compared with untreated plot. The results indicated that pathogen density was suppressed by S8 and the stain has great potential as a biological control agent for the large patch.

Morphological Characteristics and Occurrence of Yellow Tuft on Zoysiagrass (Zoysia japonica) in Cultivation Fields (들잔디 재배지에 발생한 총생 증상 및 형태적 특성)

  • Cheon, Chang Wook;Han, Jung Ji;Kim, Dong Soo;Kwak, Youn-Sig;Bae, Enu Ji
    • Weed & Turfgrass Science
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    • v.5 no.1
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    • pp.17-22
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    • 2016
  • Yellow tuft symptoms of a dense cluster on zoysiagrass (Zoysia japonica Steud.) occurred extensively at cultivated fields of zoysiagrass sods in Jangsung. The dense cluster of zoysiagrass showed significant morphological changes such as the tufts of shortening of internodes. The disease symptom was spread on a large scale throughout stolon nodes with multiple short leaves and it thrives in broom-like shaped clusters, exhibiting light green or yellow color on their leaves. The dense cluster of zoysiagrass had approximately 5.8 times more leaves on each node of its stolon then healthy zoysiagrass. Also, these zoysiagrass had poorly developed root and stolon caused by the tufts of a dense cluster of shoots. The dense cluster of zoysiagrass were collected for the putative causal agent incubation and upon close observation, it was found that the sporangia took the shape of a lemon, each sporangium was pointed at the end of its axis and was measured to be $60{\sim}96{\times}42{\sim}51{\mu}m$. These findings were analogous to the mycological characteristics of sporangia formed by the pathogen Sclerophthora macrospora. The symptoms of yellow tuft were prevalent in spring and autumn. Therefore, this study aims to present fundamental data in relation to yellow tuft on zoysiagrass in Korea.

A Survey of Weed Occurrence and Management on Apple Orchard Fields in Chungnam Province in Korea (충남지역 사과원의 잡초관리방법과 잡초발생특성)

  • Hwang, Ki Seon;Park, Kee Woong
    • Weed & Turfgrass Science
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    • v.5 no.1
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    • pp.5-9
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    • 2016
  • This survey was conducted to identify weed occurrence and to investigate weed management methods in the apple orchard fields in Chungnam province including Daejeon and Sejong from April to June and from September to October, 2015. In this survey, 64 weed species in 27 families including 39 annuals and 25 perennials were identified. Based on the importance values, the most dominant weed species in the first survey was Poa annua (6.51), followed by Veronica didyma (5.36), Plantago asiatica (5.36). In the second survey, Stellaria media (5.73), Digitaria ciliaris (5.36), and Rumex crispus (5.18) were dominant. When the 64 weed species were classified by family, the most abundant weed species belong to Compositae (12 species), followed by Poaceae (7 species), Polygonaceae (6 species), and Cruciferae (6 species). These 31 weed species in the most occurred four families accounted for 48% of total weed occurrence. Based on the questionnaire survey in which weed management methods in the apple orchard were asked, applied mowing + herbicide, mowing + tillage, and mowing + sod-culture was methods commonly conducted in apple orchard fields.

Abstraction Mechanism of Low-Level Video Features for Automatic Retrieval of Explosion Scenes (폭발장면 자동 검출을 위한 저급 수준 비디오 특징의 추상화)

  • Lee, Sang-Hyeok;Nang, Jong-Ho
    • Journal of KIISE:Software and Applications
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    • v.28 no.5
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    • pp.389-401
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    • 2001
  • This paper proposes an abstraction mechanism of the low-level digital video features for the automatic retrievals of the explosion scenes from the digital video library. In the proposed abstraction mechanism, the regional dominant colors of the key frame and the motion energy of the shot are defined as the primary abstractions of the shot for the explosion scene retrievals. It is because an explosion shot usually consists of the frames with a yellow-tone pixel and the objects in the shot are moved rapidly. The regional dominant colors of shot are selected by dividing its key frame image into several regions and extracting their regional dominant colors, and the motion energy of the shot is defined as the edge image differences between key frame and its neighboring frame. The edge image of the key frame makes the retrieval of the explosion scene more precisely, because the flames usually veils all other objects in the shot so that the edge image of the key frame comes to be simple enough in the explosion shot. The proposed automatic retrieval algorithm declares an explosion scene if it has a shot with a yellow regional dominant color and its motion energy is several times higher than the average motion energy of the shots in that scene. The edge image of the key frame is also used to filter out the false detection. Upon the extensive exporimental results, we could argue that the recall and precision of the proposed abstraction and detecting algorithm are about 0.8, and also found that they are not sensitive to the thresholds. This abstraction mechanism could be used to summarize the long action videos, and extract a high level semantic information from digital video archive.

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Development of Herbicide Resistant Plant Through Plant Tissue Culture (제초제(除草劑) Butachlor 및 Simetryne에 저항성(抵抗性)인 식물체(植物體) 선발육성(選拔育成))

  • Kim, K.U.;Kim, S.H.
    • Korean Journal of Weed Science
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    • v.7 no.2
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    • pp.200-207
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    • 1987
  • This study was conducted to select and develop herbicide resistant plant through tissue culture. Growth response of seedlings and callis of various rice varieties with Echinochloa species was assessed under the treatment of various rates of butachlor [N-(butoxy methyl)-2-chloro-2', 6'-diethyl acetamide] and simetryne [2,4-bis(ethyl amino)-6-methyl thio-1,3,5-triazine]. Further, succinate dehydrogenase activity was determined in herbicide treated callus to characterize different response of plants to herbicide. Rice variety like Sangpung showed relative resistance in both callus and seedling states against butachlor, indicating maintenance of resistance. However, in the simetryne treatment, the similar response was not observed in callus and seedling state, although there was a great different response among plant materials against simetryne. Rice variety which exhibited resistance in callus and seedling states showed low succinate dehydrogenase inhibition index. Succinate dehydrogenase inhibition index can be used as an important marker characters to differentiate varietal response of plant to herbicide. Rice plant was differentiated from butachlor and simetryne tolerant callus treated at $2.5{\times}10^{-5}$ M is growing under the growth chamber and can be used for resistant source.

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Bud sprouting and Tuberization of Eleocharis kuroguwai Ohwi (올방개(Eleocharis kuroguwai Ohwi)의 맹아(萌芽) 및 괴경형성(塊莖形成)에 관(關)한 연구(硏究))

  • Kim, Kil-Ung;Kwon, Soon-Tae
    • Korean Journal of Weed Science
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    • v.5 no.1
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    • pp.43-49
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    • 1985
  • This study was conducted to investigate the existance of dormancy in newly collected tubers of Eleocharis kuroguwai Ohwi from paddy fields and to determine the effect of various growth regulators on the bud sprouting and tuberization of E. kuroguwai. The maximum percent sprouting of E. kuroguwai collected at every month during winter was less than 60% regardless of varied collection tunes until 50 days after incubation, suggesting the presence of dormancy in E. kuroguwai tubers. This dormancy was markedly broken as the time went by, probably due to the exposure of tubers the extremly low temperature. The treatment of $BA10^{-3}M$ and $BA10^{-3}+GA10^{-6}M$ increased bud sprouting about 10 and 11 times, respectively, than that of the untreated control indicating that BA seems to be one of the most effective agents among the growth regulators used on the bud sprouting of E. kuroguwai. Foliar application of BA increased tuberization of E. kuroguwai by an average of 34.4% as compared with the untreated control. The highest increase, 39.8% was obtained with BA treatment at $10^{-5}M$.

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Antagonistic Mode of Action of Fenoxaprop-P-ethyl Phytotoxicity with Bentazon (Fenoxaprop-P-ethyl의 제초활성에 대한 Bentazon의 길항작용기구)

  • Ma, S.Y.;Kim, S.W.;Chun, J.C.
    • Korean Journal of Weed Science
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    • v.18 no.2
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    • pp.161-170
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    • 1998
  • Antagonistic mode of action of fenoxaprop-P-ethyl [ethyl(R)2-4-{(6-chloro-2-benzoxazolyloxy) phenoxy}propionate] with bentazon was investigated with respect to absorption, translocation, metabolism, and change in target site response of fenoxaprop-P-ethyl using four-leaf stage of rice(Oryza sativa L.) and barnyardgrass [Echinochloa eras-galli (L.) P. Beauv.]. Shoots of rice and barnyardgrass was more sensitive to fenoxaprop-P-ethyl than the roots. More than 90% of fenoxaprop-P-ethyl was absorbed within 6 hours after treatment and 30% of the absorbed was acropetally and basipetally translocated at 24 hours after treatment. Fenoxaprop-P-ethyl was rapidly transformed to its acid form, fenoxaprop(2-[4-(6-chloro-2-benzoxazolyloxy)phenoxy]propionic acid), which was subsequently metabolized to polar conjugates. However, changes in absorption, translocation, and metabolism of fenoxaprop-P-ethyl by bentazon treatment were not found in both species. Background activity of acetyl-CoA carboxylase(ACCase) in rice and barnyardgrass was 26.5 and 23.2nmol/min/mg, respectively. Concentration required to inhibit fifty percent enzyme activity$(I_{50})$ in vitro was 6.5~7.4${\mu}M$ of fenoxaprop-P-ethyl and more than 500${\mu}M$ of bentazon. There were no significant differences in $I_{50}$ value between two treatments of fenoxaprop-P-ethyl alone and its bentazon mixture. However, bentazon reduced ACCase activity in vivo and inhibited electron transport in chloroplast thylakoid. Based on the results obtained, it is concluded that the antagonistic effect of bentazon occurs due not to direct effect on target site of fenoxaprop-P-ethyl, but to indirect involvement in reducing herbicidal activity of fenoxaprop-P-ethyl through physiological disturbances caused by bentazone at whole chloroplast level.

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A Study of Mode of Action of Alachlor III. Effect of Alachlor on Cell Division, Cell Kinetics, Cell Elongation, and Cell Differentiation in Oat (Avena sativa L.) (Alachlor의 제초기구(除草機構)에 관(關)한 연구(硏究) III. Alachlor가 귀리의 세포분열(細胞分裂), Cell kinetics, 세포신장(細胞伸長) 및 분화(分化)에 미치는 영향(影響))

  • Kwon, S.W.;Kim, J.C.
    • Korean Journal of Weed Science
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    • v.12 no.2
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    • pp.102-109
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    • 1992
  • There was significant reduction in the mitotic indices of oat roots treated with alachlor. Uniform decrease in prophase, metaphase, anaphase, and telophase as treatment time increasing was observed. Alachlor did not disrupt mitosis, but rather inhibited the onset of mitosis. Labeled dividing cells were significantly inhibited, but the number of labeled interphase cells of all treatment were increased, as compared with control in 8 hr and 12hr period. Labeled dividing cells which entered mitosis thru $G_2$ were inhibited approximately 68% at 8hr after treatment with $1{\times}10^{-5}$ M of alachlor. Alachlor apparently inhibited from the $G_2$stage into mitosis of dividing cells. After 24 hr treatment, 12.1% abd 46.6% inhibition of coleoptile growth occurred at $1{\times}10^{-5}$ M and $1{\times}10^{-4}$ M, respectively. Cell elongation was inhibited by alachlor but was less sensitive than cell division. The longitudinal section cells of oat roots treated with $1{\times}10^{-4}$ M alachlor for 12 hr were observed to be enlarged central cylinder and also showed degradation of apical meristem zone, as compared with the untreated roots.

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