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Effects of γ-Irradiation from Cobalt-60 on pathogenicity of Eimeria tenella (Cobalt-60 감마선 조사가 Eimeria tenella 의 병원성에 미치는 영향)

  • Youn, Hee-jeong;Kang, Young-bai;Jang, Du-hwan
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.649-655
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    • 1993
  • A series of experiments on the effects of ${\gamma}-irradiation$ was performed to reveal the pathogenicity of ${\gamma}-irradiated$ oocysts of E tenella from Cobalt-60 and its progeny. The SPF chickens were inoculated with differnt doses of radiation and inoculum. The level of 100 Gy ${\gamma}-irradiation$ from $^{60}Co$ and the level of inoculum with $1{\times}10^4$ oocysts were recognized more pathogenic than those of the other groups by comparison of body weight gains, blood in feces and lesion scores. The signs of blood in feces, lesion score and the number of excreted oocysts in the feces were revealed as the lowest in the group of the ${\gamma}-irradiated$ oocysts, the average in the group of the 1st and the 3rd progeny, and the highest in the group of non-irradiated oocysts of E $tenell\grave{a}$. The body weight gain of the group immunized with ${\gamma}-irradiated$ oocysts of E tenella was higher than those of the non-irradiated, the 1st and 3rd progeny groups. The body weight gain of the groups immunized with the 1st and the 3rd progeny of E tenella were higher than that of the non-irradiated group. The feed conversion ration of the group immunized with ${\gamma}-irradiated$ oocysts of E tenella was lower than those of the non-irradiated, the 1st and the 3rd progeny groups. The feed conversion ratios of the group immunized with the 1st and 3rd progeny of ${\gamma}-irradiated$ oocysts were lower than that of the group infected with non-irradiated E tenella. The anticoccidial index(ACI 190.6) in the chickens immunized with the ${\gamma}-irradiated$ oocysts of E tenella and those(ACI 142.8 and 107.4) of the 1st and the 3rd progeny groups were higher than that (ACT 87.4) of the group infected with non-irradiated E tenella. It was thought that the pathogenicity of ${\gamma}-irradiated$ E tenella would be recovered according to increase the number of generation passaged in chicken.

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Effects of Weed Removal Time on Growth and Yield of Maize (잡초제거(雜草除去) 시기(時期)가 옥수수의 생육(生育) 및 수량(收量)에 미치는 영향(影響))

  • Kang, Y.K.;Park, K.Y.;Jong, S.K.;Park, S.U.;Moon, H.G.
    • Korean Journal of Weed Science
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    • v.5 no.1
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    • pp.50-55
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    • 1985
  • Effects of weed removal time on maize growth and yield were evaluated in the fields dominated with Poligonum spp., Chenopodium album, Echinochloa crusgalli and Digitaria adsendens from 1982 to 1983 in Suweon. Weed removal treatments were as follows: (a) kept weedfree all season, (b) weeded at 10 day intervals after maize emergence but stopped weeding at 3,5,7,9 leaf stages, (c) weeds initially removed at 3,5,7 and 9 leaf stages and the plots then kept weed free, (d) weeds left all season. Maize kept weed free from maize emergence to 7 leaf stages showed no grain yield reduction due to competition from weeds emerging later, but weeding from emergence to 3 and 5 leaf stages reduced grain yield 46 and 44%, respectively, in 1982 and 29 and 18%, respectively, in 1983. Weeding at 3 leaf stage and thereafter did not influence grain yield but initial weedings at 5, 7 and 9 leaf stages reduced grain yield 10, 36, and 67%, respectively, in 1982 and 14,18, and 21%, respectively, in 1983. Responses of maize to weeding time in the dry matter yields were same as in the grain yields.

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Lodging Related Traits and Yield of Two Rice Varieties as Affected by Paclobutrazol at Different N Levels and Split Rates (질소(窒素) 시비량(施肥量)과 분시비율(分施比率)이 다른 조건(條件)에서 Paclobutrazol 처리(處理)가 출수후(出穗後) 수도품종(水稻品種)의 도복(倒伏) 관련형질(關聯形質)과 수량(收量)에 미치는 영향(影響))

  • Lee, Suk-Soon
    • Korean Journal of Weed Science
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    • v.8 no.1
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    • pp.64-70
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    • 1988
  • An experiment was carried out to know the effects of paclobutrazol on the lodging related traits and yield of a japonia (Seomjinbyeo) and an indica (Samgangbyeo) rice variety grown at 3 N levels (10, 20, 30 kg/10a) and 3 N split rates [basal + top dressing 15 days after transplanting (DAT) : top dressing 25 days before heading (DBH) was 100 : 0, 80 : 20, and 60 : 40]. Paclobutrazol (3kg/10a of 0.6% G) was applied 15 DBH and lodging related traits were observed 3, 13, 23, and 33 days after heading (DAH). The results obtained summarized as follows. 1. Culm length was increased as N level increased, but decreased by application of paclobutrazol. Culm length was not affected by N split rates in both varieties. 2. Lodging was not occurred at all plots, but lodging index of Seomingbyeo was much greater than that of Samgangbyeo. In both varieties loding index increased up to 23 DAH due to an increase in fresh weight and a decrease in breaking strength and levelled off thereafter due to a decrease in both fresh weight and breaking strength. 3. Culm length of Seomjinbyeo and fresh weight of shoot and culm length of Samgangbyeo were positively correlated with lodging index, but breaking strength was not correlated with lodging index in both varieties during the most of ripening stages. Direct effect of culm length contributing to lodging index was positive and much greater than that of fresh weight and breaking strength in Seomjinbyeo, but in Samganbyeo direct effect of culm length and fresh weight was positive, but that of breaking strength was negative with similar magnitudes. 4. Yield in brown rice of Seomjinbyeo was not affected by N level, but split application of N increased yield. In contrast, yield of Samgangbyeo was increased as N level increased, but yield was not affected by N split rates. Paclobutrazol did not affect yield of rice in Seomjinbyeo, but decreased yield by 5.2% in Samgangbyeo due to a decrease in the number of spikelets per panicle.

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Studies on the Environmental Factors Affecting Growth and Tuber Formation of Eleocharis kuroguwai Ohwi (올방개(Eleocharis kuroguwai Ohwi)의 생장(生長)과 괴경형성(塊莖形成)에 미치는 환경요인(環境要因))

  • Ku, Y.C.;Choung, S.G.
    • Korean Journal of Weed Science
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    • v.13 no.1
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    • pp.44-54
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    • 1993
  • This experiment was conducted to understand the environmental factors affecting growth and tuber formation such as temperature, day length, tight intensity, water condition and cutting time of Eleocharis kuroguwai Ohwi. Plant height, shoot number and dry weight of E. kuroguwai were higher at high temperature, 25/$25^{\circ}C$ (day/night), while nitrogen content was higher at low temperature, 20/$15^{\circ}C$. Plant height was more affected by water temperature, while shoot number and dry weight were more affected by air temperature. Contents and absorption of nitrogen, phosphorus, and potassium in top parts of E. kuroguwai were higher under greater difference between air and water temperatures, i.e., 18/$28^{\circ}C$ and 28/$18^{\circ}C$. The number and weight of tubers were increased under greater difference between air and water temperatures, i.e, 18/$28^{\circ}C$ and 28/$18^{\circ}C$, while they were inhibited at low or high air/water temperatures (18/$18^{\circ}C$ or 28/$28^{\circ}C$). Tubers of E. kuroguwai were formed at 8-or 12-hour day length, however, no tuber was formed at l6-hour day length. Photoinductive period for tuber initiation of E. kuroguwai was between 30 and 45 days after emergence, and the induction period of short-day treatment was less than 10 days. Tuber number and weight were reduced by shading due to inhibition of the growth of top and underground parts. Number of days from planting to tuber initiation was shortned as planting time was delayed and plant height, dry weight, and tuber number were also reduced by delayed planting. Tuber number at l0 to 15cm water depth was decreased 63 to 75% as compared with 1 to 5cm water depth. Tuber number and dry weight were not affected by the size of tubers at planting. Due to the reduced growth of top and underground parts, tuber number and dry weight of E. kuroguwai were decreased by delayed shoot cutting. The critical cutting time to inhibit the growth of E. kuroguwai was about 70 days after emergence.

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Studies on the Constituents and Culture of the Higher Fungi of Korea(II) -The Antitumor Components and Culture of Lentinus edodes(Berk.) Singer- (한국산(韓國産) 고등균류(高等菌類)의 성분(成分) 및 배양(培養)에 관한 연구(硏究)(II) -표고버섯의 항암성분(抗癌成分) 및 배양(培養)-)

  • Chung, Kyeong-Soo
    • The Korean Journal of Mycology
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    • v.10 no.1
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    • pp.33-39
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    • 1982
  • Carpophores of ten Korean strains of Lentinus edodes (Berk.) Singer, an antitumor polysaccharide producing fungus, were extracted with 0.1N NaOH solution. The extracts were dialized for seven days in distilled water and lyophilized to produce crude polysaccharide powders. Thus obtained crude polysaccharide samples were assayed for sugar contents by colorimetric method with anthrone reagent. Among ten strains examined Lentinus edododes-DMC7 was found to be the richest strain in polysaccharide content of carpophores. By shake culture experiment for biomass production, L. edodes-DMC7 was found to be the second most productive strain among seven strains examined. Cultural characteristics of L. edodes-DMC7 were investigated by shake culture method. The best result was obtained when L. edodes-DMC7 was cultured in the medium containing glucose 8g, starch 80g, yeast extract 12g, $KH_2PO_4\;0.87g,\;MgSO_4{\cdot}7H_2O\;O.5g,\;CaCl_2\;0.3g,\;FeSO_4{\cdot}7H_2O\;10mg\;ZnSO_4{\cdot}7H_2O\;4mg,\;CuSO_4{\cdot}5H_2O\;lmg,\;MnCl_2{\cdot}4H_2O\;7mg\;per\;11\;at\;28^{\circ}C$, 180 rpm, for 12 days. Thus thirty-three grams of dry mycelia was obtained per one liter of medium.

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Synthesis of Ultra High Refractive Index Monomer for Plastic Optical Lens and Its Ophthalmic Lens Preparation (플라스틱 안경렌즈용 초고굴절 모노머 합성 및 이를 이용한 안경렌즈 제조)

  • Jang, Dong Gyu;Kim, Jong Hyo;Lee, Soo Min;Roh, Soo Gyun
    • Journal of Korean Ophthalmic Optics Society
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    • v.13 no.3
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    • pp.1-6
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    • 2008
  • Purpose: Plastic optical monomer materials having ultra high refractive index have an income of the whole quantity from advanced nations to domestic companies which are related to plastic optical lens. It is necessary to develop novel plastic optical lens materials in order to overcome a FTA provision and revitalize a stagnating optical lens industry in the interior optical lens industries. The new plastic optical lens materials against the substitution effect of income should be gradually demanded. This work will be synthesized novel super high refractive monomer resin materials of urethane lens series and studied the properties of optical lens using it. Methods: ETS-4 (2-(2-mercaptoethylthio)-3-{2-[3-mercapto-2-(2-mercaptoethylthio)propyl thio]ethylthio}propane -1-thiol), which is optical lens monomer resin having super high refractive index, was synthesized and identified its structure and property by elemental analysis, EI-MS, TGA, FT-IR spectroscopy, $^1H$ and $^{13}C$ NMR spectroscopies. After mixing evenly from mixed monomer resin and diisocyanate series, it was casting in glass mold. After thermal curing, the obtained optical lenses were measured and compared with the refractive index and Abbe number for studies of their optical properties. Results: We have synthesized the novel ultra high refractive index monomer resin, ETS-4, and have identified its structure and property by elemental analysis, EI-MS, TGA, FT-IR spectroscopy, $^1H$ and $^{13}C$ NMR spectroscopies. The existence of three isomers for EST-4 was identified by $^{13}C$ NMR spectroscopy. The refractive index ($N_d$ at $25^{\circ}C$) of monomer resin in liquid state obtained from the Abbe refractometer was 1.647. The refractive indexes of raw plastic optical lenses prepared from the mixed ETS-4 monomer and diisocyanate series were in the range of 1.656~1.680. Conclusions: Novel super high refractive index plastic optical lens monomer was synthesized and analysed, the optical lenses prepared using it were colorless transparency and excellent properties. It is of utility for the industrialization.

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Studies on the Surface Charge Characteristics and Some Physico-Chemical Properties of two Synthetic Iron Hydrous Oxides and one Aluminum Hydroxide Minerals (합성(合成) 수산화(水酸化) 철(鐵) 광물(鑛物)과 수산화(水酸化) 알루미늄 광물(鑛物)의 표면(表面) 전하(電荷) 및 물리화학적(物理化學的) 특성(特性)에 관(關)한 연구(硏究))

  • Lim, Sookil H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.17 no.2
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    • pp.147-154
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    • 1984
  • Two Fe-hydrous oxide A,B and one Al-hydroxide minerals were synthesized precipitating Fe $Cl_3$ and $AlCl_3$ with alkali solution(NaOH) at pH 6.0, 12.0 and 4.5 respectively, for precise understanding of physico-chemical and surface charge characteristics of soils in which these minerals are dominant. Identification of these final products, effect of free and amorphous materials on X-ray diffraction analysis, particle size distribution and surface change characterics of these minerals were performed. Fe-hydroxide A and B were identified as great deal of X-ray amorphous material and as goethite with large amount of X-ray amorphous material, respectively. Dehydration by oven at $105^{\circ}C$ of these minerals exhibited akaganeite peaks with low X-ray amorphous hump and pure goethite peaks for Fe-hydroxide A and B, respectively. Both minerals, however, turned into hematite upon firing at $550^{\circ}C$. On the other hand, Al-hydroxide identified as mixture of gibbsite and bayerite of around 7:3 ratio. Application of sodium dithionite and ammonium oxalate solutions for removal of free or amorphous Fe and Al from these minerals revealed that only peak intensities of Al-hydroxide system were enhanced upon Al-extraction by oxalate solution even though dithionite solution was much powerful to extract Fe from Fe-hydrous oxide systems. Original(wet) Fe-hydrous oxide A has the highest specific surface and surface charge development(negative and positive), and the greatest amount of less than $2{\mu}m$ sized particles. Specific surface and clay sized particles(less than $2{\mu}m$) of Fe-hydrous oxide A, however, were drastically reduced upon dehydration($P_2O_5$ and oven drying) compare to the rest minerals. The Z.P.C. of these synthetic minerals were 8.0-8.5, 7.5-8.0 and 5.5-6.0 for Fe-hydrous oxide A, B and Al-hydroxide, respectively.

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Effect of long-term organic matter application on physico-chemical properties in paddy soil -I. Changes of the soil-chemical properties of paddy field by long-term application of organic matter (논토양(土壤)의 이화학적(理化學的) 성질(性質)에 미치는 유기물(有機物)의 연용효과(連用效果) -I. 유기물연용(有機物連用) 답(畓)의 토양화학적(土壤化學性) 변화연구(變化硏究))

  • Yoo, Chul-Hyun;Kim, Jong-Gu;Park, Keon-Ho;Kim, Seong-Jo
    • Korean Journal of Soil Science and Fertilizer
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    • v.21 no.3
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    • pp.264-271
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    • 1988
  • This experiment was carried out to investigate the effects of long-term applications of organic matters on the chemical properties and on the application levels of nitrogen in Fluvio-Alluvial plain of Jeonbug series. The amounts of application of rice straw and compost, the sources of organic matters, were 500 and 1,000 kg/10a in combined with the different nitrogen levels of 0.15 and 20 kg/10a, respectively. The results obtained from the 9 year's experiment during 1979 to 1987 were summarized as follows: I. In long-term application of Organic matter the soil pH showed the lowest value in the 3rd-4th year at rice straw and 5th-6th year at compost but it varied less in control plot for 9 years. 2. Organic matter content in the soils was gradually increased by yearly application of organic matter, while it was higher in rice straw than in compost since the 5th year. 3. The contents of available silica and available phosphate in soil were rapidly increased by long-term application of organic matter and it was especially higher in rice straw than compost, but it was gradually decreased in control plot. 4. The contents of exchangable cations (Ca, Mg and K) and the total nitrogen were increased by long-term application of organic matter they were in rice straw than in compost. But they showed decreasing tendency in control plot. 5. The soil Eh was lower in order of rice straw, compost and control plot however application of orgnic matter resulted in increasing soil Eh due to the rapid reduction of soil from panicle formation to heading stage in rice cultivation. 6. The number of panicles per $m^2$ and the number of granis per panicle were increased by increment of nitrogen levels in all treatments and especially largest in rice straw application. But 1,000 grain weight increased in low nitrogen level with long-term applications of organic matter. 7. It was estimated that the application levels of nitrogen by long-term application of organic matter were 21, 24 and 20 kg/10a for 1st-3rd, 4th-6th and 7th-9th year in rice straw application, and 16 and 19 kg/10a for 1st-3rd and 4th-9th year in compost application, respectively.

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The Implementation and limits of Involuntary Detention of the Tuberculosis Prevention Act (결핵예방법의 격리명령의 실행과 한계에 관하여)

  • Kim, Jang Han
    • The Korean Society of Law and Medicine
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    • v.16 no.2
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    • pp.55-84
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    • 2015
  • The tuberculosis is the infectious disease. Generally, the active tuberculosis patient can infect the 10 persons for one year within the daily activities like casual conversation and singing together. The infectivity of tuberculosis can continue for a life time, and infected persons can remain at risk for developing active tuberculosis. To control this contagious disease, along with the active tuberculosis patients, non-infectious but non-compliant patients who can be infectious if their immune systems become impaired have to be managed. To control the non-complaint patients, medical treatment order should be combined with the public order. Because tuberculosis is the risk of community health, the human rights like liberty and freedom of movement can be restricted for public welfare under the article 37(2) of constitution. Even when such restriction is imposed, no essential aspect of the freedom or right shall be violated. The degree of restriction on the rights of citizens is different what methods are chosen to non-complaint patients. For example, under the directly observed therapy program, the patients and medical staffs make an appointment and meet to confirm the drug intakes according to the schedule, which is the medical treatment combined with the mildest public order. If the patients break the appointments or have the history of disobedient, the involuntary detention can obtain the legitimate cause. The Tuberculosis Prevention Act has the two step programs on this involuntary detention, The admission order (Article 15) is issued when the patients are infectious. The quarantine order (Artle 15-2) is issued when the patients are infectious and non-complaint. The legal criteria for involuntary detention are discussed and published through the international conventions and covenants. For example, World Health Organization had made guidance on human rights and involuntary detention for tuberculosis control. The restrictions should be carried out in accordance with the our law and in the legitimate objective of public interest. And the restriction should be based on scientific evidence and not imposed in an unreasonable or discriminatory manner. We define and adopt these international criteria under our constitution and legal system. Least restrictive alternative principle, proportionality principle and the individual evaluation methods are explained through the reviews of United States court decisions. Habeas Corpus Act is reviewed and adopted as the procedural due process to protect the patient rights as a citizen. Along with that, what conditions and facilities which are needed to performed quarantine order are discussed.

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Standards of Due Diligence and Separation of Responsibilities in the Division of Labor in Medicine (분업적 의료행위에 있어서 주의의무위반 판단기준과 그 제한규칙들)

  • Choi, Hojin
    • The Korean Society of Law and Medicine
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    • v.19 no.2
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    • pp.41-72
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    • 2018
  • In the division of labor (or teamwork) in medicine, the responsibility of medical and nursing staff should be separated or distributed to justify negligent criminal offenses. The present work refers to the standards by which the due diligence and responsibility of the individual persons are to be determined and delimited. In this context, it has been proven that objective theory as a measure of due diligence is appropriate. From a moral point of view, when assessing due diligence, it makes sense to impose greater individual or higher performance demands on the perpetrator, but law and order require that due diligence should result from socially relevant human behavior. To give objective measure of negligence and to provide the highest level of personal responsibility, so that man can not be burdened too much responsibility and it is accordingly with an equality theorem. Afterwards some points are presented, which should be considered in a concrete fact in the determination of the medical negligence. Medical action has specific characteristics such as professionalism, discretionary and exclusive, unbalance of information. These characteristics distinguish medical actions from general negligence. The general level of knowledge, the urgency, working condition and working environment of the medical facility, duration of the professional practice, assessment of the medical activity are crucial in this context. As a standard of delineation of due diligence, I have used the permitted risk and the principle of trust. In the horizontal division of labor, the principle of trust applies. The principle of trust applies in principle in cases of division of labor interaction, when doctors in the same hospital exercise their own specific occupational field or everyone works in another hospital. However, this is not true for every case. In the vertical division of labor, the principle of trust does not apply and the senior physician can not trust the assistant doctors. In this case, the principle of trust is converted into a duty of supervision for assistant doctors by the senior physician. This supervision requirement could be used as a random check.