• Proceedings of the Korean Biophysical Society Conference
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• 1999.06a
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• pp.39-39
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• 1999

Single chain-monellin (SCM) was recently constructed by fusing the two chains of monellin. From the view of protein folding, SCM serves as an ideal model system especially in tackling ${\alpha}$-helix-${\beta}$-sheet interactions due to the following reasons： First, it consists of simple distinct structural elements (${\alpha}$-helix and ${\beta}$-sheet) which are assembled in a perpendicular manner.(omitted)

• Journal of KIISE:Software and Applications
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• v.29 no.11
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• pp.826-836
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• 2002

We need an enormous amount of memories for name spaces as well as additional processors if we are to effectively exploit a massively parallelism in nested loops of functional languages such as Id. If there is no sufficient amount of memories enough to exploit that parallelism, the execution of programs can be aborted during the unfolding of loops. Additionally, if loops are overunfolded, compared with the number of processors available, the system performance can be degraded severely due to the overhead of loop unfolding. This paper suggests and analyzes an algorithm which can be used to effectively unfold nested loops of functional languages on multithreaded architectures. This algorithm has a feature to unfold a given nested loop safely and near optimally, considering the system resources of processors and memories available when the loop is to be unfolded.

• The Journal of Korean Association of Computer Education
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• v.23 no.4
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• pp.35-47
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• 2020

The purpose of this study is to provide a guideline for the programming beginners, including SW non-majors, to reduce the difficulty of establishing procedures for solving problems and to refine the work process properly in a computing environment. To accomplish this, we derive the unfolding types of typical procedures that can unfold the working procedures typically implied in daily operation in terms of recognition(input)-judgment(processing)-behavior(output). Through learning to unfold the procedure for each type, it was confirmed that the learner define the scope and rules of the problem himself and extended the procedure implied in any action. The unfolding model of the procedure developed in this study can be used as a tool for constructing a procedure operable in a computing environment to solve problems in the early stages of programming learning for non-majors or beginners.

• BMB Reports
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• v.37 no.5
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• pp.586-596
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• 2004

The folding of ervatamin C was investigated in the presence of various fluorinated and non-fluorinated organic solvents. The differences in the unfolding of the protein in the presence of various organic solvents and the stabilities of O-states were interpreted. At pH 2.0, non-fluorinated alkyl alcohols induced a switch from the native $\alpha$-helix to a $\beta$-sheet, contrary to the $\beta$-sheet to $\alpha$-helix conversion observed for many proteins. The magnitude of ellipticity at 215 nm, used as a measure of $\beta$-content, was found to be dependent on the concentration of the alcohol. Under similar conditions of pH, fluorinated alcohol enhanced the intrinsic a-helicity of the protein molecule, whereas the addition of acetonitrile reduced the helical content. Ervatamin C exhibited high stability towards GuHCl induced unfolding in different O-states. Whereas the thermal unfolding of O-states was non-cooperative, contrary to the cooperativity seen in the absence of the organic solvents under similar conditions. Moreover, the differential scanning calorimetry endotherms of the protein acquired at pH 2.0 were deconvoluted into two distinct peaks, suggesting two cooperative transitions. With increase in pH, the shape of the thermogram changed markedly to exhibit a major and a minor transition. The appearance of two distinct peaks in the DSC together with the non-cooperative thermal transition of the protein in O-states indicates that the molecular structure of ervatamin C consists of two domains with different stabilities.

• Journal of the Korean Chemical Society
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• v.55 no.5
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• pp.746-750
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• 2011

Fluorescence correlation spectroscopy (FCS) is an emerging fluorescence technique used to study the dynamics of proteins on a millisecond to microsecond time scale at the single-molecule level. Solution pH-modulated protein conformational changes can be manifested by binding rate, fluorescence lifetime, and binding specificity of a probe molecule. The fluorescence lifetime of Nile red (NR) bound to apomyoglobin (apoMb) was measured to be $6{\pm}0.3$ ns, much longer than that in water solution ($2.9{\pm}0.2$ ns). As the unfolding population of apoMb increased by lowering pH of solution, the fraction for the longer lifetime of NR decreased with an increasing fraction for the shorter lifetime of NR in water. Unlike 1-anilino-8-naphthalene sulfonic acid, which has many lifetimes due to nonspecific binding to the unfolded apoMb, NR bound to apoMb possesses only a single lifetime. These results suggest that NR binds specifically to native apoMb and thus can be utilized to probe the folding/unfolding dynamics of apoMb using FCS.

• Genomics & Informatics
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• v.4 no.3
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• pp.133-136
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• 2006

The adsorption of proteins on the surface of glass slides is essential for construction of protein chips. Previously, we prepared a nickel-coated plate by the spin-coating method for immobilization of His-tagged proteins. In order to know whether the structural factor is responsible for the immobilization of His-tagged proteins to the nickel-coated glass slide, we executed a series of experiments. First we purified a His-tagged protein after expressing the vector in E. coli BL21 (DE3). Then we obtained the unfolding curve for the His-tagged protein by using guanidine hydrochloride. Fractions unfolded were monitored by internal fluorescence spectroscopy. The ${\Delta}G_{H20}$ for unfolding was $2.27kcal/mol{/pm}0.52$. Then we tested if unfolded His-tagged proteins can be adsorbed to the nickel-coated plate, comparing with $Ni^{2+}-NTA$ (nitrilotriacetic acid) beads. Whereas unfolded His-tagged proteins were adsorbed to $Ni^{2+}-NTA$ beads, they did not bind to the nickel-coated plate. In conclusion, a structural factor is likely to be an important factor for constructing the protein chips, when His-tagged proteins will immobilize to the nickel-coated slides.

• (The) Research of the performance art and culture
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• no.18
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• pp.137-169
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• 2009

This paper pay attention to silent puppets which appear in the traditional puppet performance. Not only movement of silent puppets which are involved with expression of body of the mime, but also various methods overcome limits of the silent are studied in this paper. Through the study of performance method and historic unfolding aspects, I confirm two main points. First point is that overcome limits of the silent, through the diversity of performance aspects. Second point is that silent puppets have been existed since the early stage of history, and show typical unfolding aspects on their way. Combining two main points, We can say that silent puppets go through continuous unfolding aspects on their way and have meaning of existence. The meaning of existence are that silent puppets seek deviant desire which deviate from everyday life and transcendental desire which seek a freedom through an imagination or an idea. It is interesting that the reasons of existence are associated with the emphasis of necessity which is encouragement of audience's desire and imagination that are necessary of accomplish of mime. After all, silent puppets say that diverse attempts which overcome limits of the silent are accomplished with the performance aspects and historic unfolding aspects, and through that sought unusual communication. Through the performance methods and signification aspects, silent puppets say that diverse groping for which maximize of audience's imagination, stimulation of imagination for audience's spontaneousness, and diverse groping for audience mind performance have been performanced as well. I think that those are the voice of silent puppets for activity of mime in the korea.

• Bulletin of the Korean Chemical Society
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• v.19 no.6
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• pp.608-611
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• 1998

• IEIE Transactions on Smart Processing and Computing
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• v.5 no.1
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• pp.29-34
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• 2016

A controller area network (CAN) controller is an integral part of an electronic control unit, particularly in an advanced driver assistance system application, and its characteristics should always be advantageous in all aspects of functionality especially in real time application. The cost should be low, while maintaining the functionality and reliability of the technology. However, a CAN protocol implementing serial operation results in slow throughput, especially in a cyclical redundancy checking (CRC) unit. In this paper, digital signal processing (DSP) algorithms are implemented, namely pipelining, unfolding, and retiming the CAN controller in the CRC unit, particularly for the encoder and decoder sections. It must attain a feasible iteration bound, a critical path that is appropriate for a CAN system, and must obtain a superior design of a high-speed parallel circuit for the CRC unit in order to have a faster transmission rate. The source code for the encoder and decoder was formulated in the Verilog hardware description language.

• Journal of Life Science
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• v.9 no.6
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• pp.733-736
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• 1999

The doubly altered mutant tryptophan synthase $\alpha$-subunits, in which Thr 24 was replaced by Ser, Leu or Lys in addition to F139W substitution, were purified. Urea-induced unfolding equilibrium curves of these proteins, monitored by fluorescence intensity of tryptophan, show that the alterations of residue 24 resulted in marked changes in folding properites, suggesting the importance of this residue in folding of this protein.