• Safety and Health at Work
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• 제14권1호
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• pp.124-130
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• 2023

The increase in the number of fatal electric accidents over the years has escalated the demand for specialized forensic engineers to determine their relevant technical causes. Likewise, the complexities associated with identifying the causes of electrocution accidents have prompted the General Department of Forensic Science and Criminology at Dubai Police to adopt a new methodology to diagnose electrocution accidents, consisting of an approach that involves medico-legal examination, electrical diagnosis of the evidence, and trace evidence analysis. This paper will discuss the application of the adopted method in further detail by unfolding two case reports. The first report outlines a case in which a worker got electrocuted at a construction site while attempting to turn on a lamp. The second case report involves the death of a technician in a workshop after trying to disconnect a washing machine from its plug. The methodology was utilized during the investigation of both cases, which were attended by the appointed forensic engineers and showed promising results.

• 대한화학회지
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• 제68권1호
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• pp.25-31
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• 2024

The p97 adenosine triphosphatase is a key player in protein homeostasis, responsible for unfolding ubiquitylated substrates. It engages with various adaptor proteins through its N-terminal domain, with the p97-p47 complex attracting particular attention for its involvement in membrane remodeling. Although the structures of p97 in complex with the Ubiquitin regulatory X (UBX) domain from various adaptors have been reported, the stoichiometry is conflicting. Here, we report the crystal structure of the p97 N-D1 hexamer in complex with the p47 UBX domain at a resolution of 2.7 Å. The structure reveals a stoichiometry of 6:6 between the p97 N-D1 and the p47 UBX domain. These findings provide valuable insights into the binding stoichiometry of p97 N-D1 and p47 UBX domain, which are crucial for understanding the role of p97 and adaptor proteins in cellular processes such as the ubiquitin-proteasome pathway, membrane fusion, and cell cycle regulation.

• 한국의상디자인학회지
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• 제14권1호
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• pp.1-11
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• 2012

The Original forms of the ancient clothing have been evolved in various designs and showed each unique formativeness based on the basic principles of origami. The purpose of this study was to analyze the basic clothing formativeness in the original forms of the ancient clothing based on the principles of origami. The methods of this study were to identify significant relevant ancient clothing figures with each unique formativeness based on the principles of origami and analyze the formativeness characters and values through comprehensive literature reviews on topic-related books and theses on a foundation of triangulation of observer. The results were as follows: The significant ancient clothing figures with principles of origami were identified as kalasiris, chiton, chlamys, toga, dalmatica, paludamentium, and lorum. The formative principles applied in the ancient clothing figures were identified as exaggerated measurements, folding and unfolding, bending, and angle. And the formative characters and values were identified as potential drape realization, potential silhouette realization, potential three-dimensional design optimality, and potential three-dimensional formativeness realization. The result of this study may be used for a newer approach for the fundamental digging of clothing formativeness in advance.

• 컴퓨터교육학회논문지
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• 제18권3호
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• pp.1-14
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• 2015

본 논문의 목적은 정보과학영재반 학생들이 계산적 사고의 관점에서 NetLogo 프로그래밍을 하면서 문제를 해결하는 과정의 특성을 살펴보는 데 있다. 이를 위하여 전남 지역의 S대 부속 과학영재교육원에서 실시한 중등정보과학 수업에 관한 강의 자료, 학생들의 프로그래밍 산출물, 소감문을 수집하였고 면담 및 학생 활동을 촬영한 비디오 자료를 추가하였다. 연구자는 NetLogo 프로그래밍 수업 중 다각별 그리기 과제에서 나타나는 학생들의 사고 및 프로그래밍 작업 패턴, 학습태도, 행동특성 등을 분석함으로써 계산적 사고와 관련된 프로그래밍 학습의 특징을 도출하였다. 연구참여자(진우)는 계산적 사고를 해 나가는 과정과 컴퓨터 프로그래밍으로 코딩하는 방식에서 번득이는 아이디어의 순간 포착 및 간결한 코딩 등의 양상을 보여주었다. 사례간의 비교고찰과 해석적 논의를 통해 중등정보과학 영재수업을 담당하는 교육실천가들에게 계산적 사고를 함양시키는 프로그래밍 교수-학습과정에 관한 후속 연구 방향을 제시하였다.

• 컴퓨터교육학회논문지
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• 제20권1호
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• pp.49-62
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• 2017

본 논문의 목적은 수학정보 영재반 수업에서 고고보드를 사용하여 고고범퍼카의 조립 과정 및 고고모니터 명령어로 고고범퍼카를 제어하는 활동을 분석하여 강사와 참여 학생 사이의 상호작용적 활동과 컴퓨팅 사고 패턴을 질적으로 탐구하는 것이다. 연구자는 연구참여자(로봇 강사 및 참여 학생) 2명을 대상으로 2013년 7월 말에 이틀 동안 고고범퍼카 프로젝트 수업을 비디오로 촬영하였다. 그리고 그 이후에 진우를 대상으로 2013년 11월과 2014년 1월 사이에 별도의 후속 면담과 비디오 회상면담을 3회 실시하여 고고범퍼카 프로젝트 수행 과정에 관한 자료를 보완적으로 수집하였다. 비디오 및 면담 자료와 산출물인 고고모니터 코드를 분석한 결과 연구자는 고고범퍼카 프로젝트의 수행 활동에서 나타나는 컴퓨팅 사고의 전개 양상을 포착하였다. 참여 학생은 고고보드 부품 사용과 고고모니터 코딩 활동에서 로봇 강사와의 상호작용을 통하여 종종 숙고하면서 자신이 생각하는 바에 해당되는 명령어의 조합을 배열하면서 수정과 테스트를 순환적으로 하는 컴퓨팅 사고의 전개 패턴을 보여주었다.

• 한국수정란이식학회지
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• 제5권1호
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• pp.1-20
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• 1990

Follicular atresia is a universal and characteristic phenomenon of both non-mammalian and mammalian vertebrates. Generally it is estimated that greater than 99% of follicles become atretic in higher domestic animals and human. The number of selected follicles developing to the preovulatory stage are thus fewer. Follicles can become atretic at any stage of development. The previous studies emphasized on descriptive and retrospect aspects of a limited population of the fully grown preovulatory follicle. The main efforts in ovarian physilogical researches are focused on follicular development culminating in ovulation but recent advances have resulted in a better understanding of atresia. Nowadays, recent studies are concentrated on the induction of atresia in a selected population of follicles and of the associated cellular, endocrine, biochemical and molecular changes. The factors initiating atresia and follicle selections are worthy of investigations. Another intriguing question is whether one can predict when a follicle will become atretic, i.e., what biochemical markers indicate that a follicle is destined for atresia. It is generally agreed that atretic process may vary even in antral follicles at different stages of their differentiations and among species. The dicisive factors are follicular responsiveness and the hormonal milieu. Some generalizations can be made on the basis of experimental induction of atresia. Alteration of the pattern of follicular steroid production is associated with the initiation stage of atretic process. Atresia appears to be a process unfolding gradually and affecting progressively in increasing number of functions and components of the follicle. The oocyte may be the latest to be afflicted in the atretic process. The high steroidogenic activity of atretic follicles lends support to the notion that atresia is not necessarily a degenerative process and that atretic follicles may play an essential role in ovarian physiology. The simultaneous occurence of growth and atretic processes may render the search for regulatory mechanisms involved in atresia difficult extremely. The questions such as how follicles are selected to undergo ovulation rather than atresia or what the mechanism of atresia is remain unanswered. However, the factors regulating or modifying ovarian hormonal milieu for the initiation of follicular growth and maturation or of atresia are being elucidated.

• Journal of Microbiology and Biotechnology
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• 제10권6호
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• pp.797-804
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• 2000

The human immunodeficiency virus type 1 (HIV-1) Tat is one of the viral gene products essential for HIV replication. The exogenous Tat protein is transduced through the plasma membrane and then accumulated in a cell. The basic domain of the Tat protein, which is rich in arginine and lysine residues and called the protein transduction domain (PTD), has been identified to be responsible for this transduction activity. To better understand the nature of the transduction mediated by this highly basic domain of HIV-1 Tat, the Green Fluorescent Protein (GFP) was expressed and purified as a fusion protein with a peptide derived from the HIV-1 Tat basic domain in Escherichia coli. The transduction of Tat-GFP into mammalian cells was then determined by a Western blot analysis and fluorescence microscopy. The cells treated with Tat-GFP exhibited dose- and time-dependent increases in their intracellular level of the protein. the effective transduction of denatured Tat-GFP into both the nucleus and the cytoplasm of mammalian cells was also demonstrated, thereby indicating that the unfolding of the transduced protein is required for efficient transduction. Accordingly, the availability of recombinant Tat-GFP can facilitate the simple and specific identification of the protein transduction mediated by the HIV-1 Tat basic domain in living cells either by fluorescence microscopy or by a fluorescence-activated cell sorter analysis.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1286-1286
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• 2001

Despite extensive theoretical and experimental studies the structure of the protein-solvent interface is subject of many controversy. Understanding the processes that occur in aqueous solution requires understanding of the solvent influence on the structure of protein. The aim of this study is to investigate the applicability of NIR methods in the study of hydration phenomena in protein solutions. Temperature-induced changes in NIR spectra of -lactoglobulin (BLG) in aqueous solutions have been investigated by means of two-dimensional correlation spectroscopy (2DCOS) and principal component analysis (PCA). With the temperature increase the balance of forces between the BLG's interaction with itself and the BLGs interaction with its environment is disrupted leading to BLG unfolding. Significant differences of 2D signals and distinct discrepancies of loading on PC1 and PC2 were observed as a result of temperature increase. In the native folded conformation of BLC, most of the nonpolar amino acids are hidden in the centre of the structure, out of contact with water molecules, while charged groups are outside, in the contact with water. The polar groups promote low density Ih-type structure in the water outside this first hydration shell. When BLG unfolds it assumes a more extended configuration on which the previously buried nonpolar groups are exposed to water and promote the higher density II-type structure outside its first shell. Detailed assignments of bands attributed to the bulk water, different states of the hydrated water and the changed conformation of BLG are proposed.

• Toxicological Research
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• 제17권
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• pp.135-138
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• 2001

Female Wistar rats were randomly divided into 5 groups: Control, received distilled water; Low lead, received 0.5 g/ιlead (as acetate) in drinking water; High lead, received 2.0 g/ιlead; Low lead + Minerals, received 0.5 g/ιlead in drinking water and received minerals (Ca$^{2+}$, 25 mg/kg/day; Fe$^{3+}$, 0.47 mg/ kg/day; Zn$^{2+}$, 0.33 mg/kg/day; Se, 0.83 $\mu\textrm{g}$/kg/day) by gavage; High lead + Minerals, received 2.0 g/ιlead and received the same minerals. Animals exposure to lead was from 10 days before mating till postnatal day 21; and the minerals was administered from the first day of pregnancy and during lactation. No statistical difference was found either in body weights or in blood lead levels between the pups received minerals and those only exposed to lead at the same dose. The developmental and behavioral teratological effects of lead on pups, such as time-lag of eye opening, pinna detachment, fur developing, incisor eruption, ear unfolding, and surface righting were observed in this study; and the minerals decreased the toxicity of lead either in low or in high lead exposure pups. The numbers of step-down were significantly increased in lead exposed animals, and the effect of intervention by the minerals was appeared only in the pups exposed to low lead. The ChAT activity and levels of glutamate and aspartate in hippocampus decreased in treated animals compared to control animals, no effect of intervention by the minerals was found. The results of this study indicate that the applied multi-minerals can alter the outcome of develop-mental lead poisoning in rats.s.s.s.

• 디자인학연구
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• 제20권
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• pp.173-182
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• 1997

이 시대에 국경 없는 글로벌 시장(Global Market)의 무한경쟁시대 직면해 있음을 인식한 국내의 유수한 기업들이 과거 내수시장을 탈피하여 국제화를 지향하기 위한 글로벌 경영을 선언하고 초일류 제품디자인 개발을 시도하고 있다. 이 같은 현상은 글로벌 화된 제품디자인을 통해 마켓쉐어(Market Share)를 상승시키는데 그 중요성에 대한 인식이 커지고 있음을 반영한 것이다. 따라서 본 연구는 글로벌 제품디자인 개발의 접근을 인류문화의 기호론(嗜好論)과 디자인 측면에서 재해석하고, 또 인류 모두가 좋아하는 공통적 기호(嗜好)를 유추 또는 수용하여 접근할 수 있는 방법을 제시하는데 목표를 두었다. 글로벌 제품디자인 접근의 구성요소인 디자인과 커뮤니케이션 관계에서 기호론 적 표현컨셉트와 아이디어를 전개하는 방법을 합리적으로 체계화 하고자 하였다. 이를 위해 기호론 적 문헌 연구를 토대로 디자인 접근방법을 디자인 프로세스에서 적용시키면서 사용자와 디자인, 기업 이념과 기호론 적 표현에서 의사전달(Communication) 접근방식을 제시하였다. 그리고 우리의 전통문화에서 형태적 아이디어를 발췌하여 접근하는 가운데 화장품 용기디자인을 사례로 하였다.