• Plant Biotechnology Reports
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• v.5 no.3
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• pp.273-281
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• 2011

Diverse epigenetic phenotypes are frequently found during research on transgenic plants. To understand the factors underlying such diversity, hundreds of independent 35S-GFP transgenic N. benthamiana plants were analyzed. The diverse GFP-expression phenotypes of the transgenic plants were classified into three major types based on the GFP expression patterns and their response to 35S-GFP agroinfiltration: steady-green, silenced and non-uniform phenotype. The non-uniform phenotype was further sub-divided into five minor phenotypes: variegated, red-dropped, on-silencing, partitioned and misty, according to the distribution of GFP expression on the leaves. Many of transgenic plants continuously generated diverse phenotypes over several generations despite the transgene identity. Such epigenetic GFP phenotyping was found to be the result of spontaneous transgene silencing mediated by either or both of post-transcriptional gene silencing (PTGS) and transcriptional gene silencing (TGS). This finding was verified by the detection of 21- and 24-nt small interfering RNA (siRNA) molecules, and DNA methylation in the transgenic plants that showed repeated epigenetic variation. Agroinfiltration demonstrated that irregular distribution of GFP on a leaf was the result of erratic transgene silencing, and the technique also proved to be a rapid and effective method for selecting fully silenced plants within 3 days. Furthermore, two novel phenotypes described are potential materials for in-depth investigations into the genes and mechanisms responsible for spontaneous transgene silencing.

• Journal of Magnetics
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• v.21 no.1
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• pp.35-39
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• 2016

$Cr_{100-x}Ti_x$ amorphous texture-inducing layers (TIL) were investigated to realize highly (002) oriented $L1_0$ FePt-C granular films through hetero-epitaxial growth on the (002) textured bcc-$Cr_{80}Mn_{20}$ seed layer (bcc-SL). As-deposited TILs showed the amorphous phase in Ti content of $30{\leq}x(at%){\leq}75$. Particularly, films with $40{\leq}x{\leq}60$ kept the amorphous phase against the heat treatment over $600^{\circ}C$. It was found that preference of the crystallographic texture for bcc-SLs is directly affected by the structural phase of TILs. (002) crystallographic texture was realized in bcc-SLs deposited on the amorphous TILs ($40{\leq}x{\leq}70$), whereas (110) texture was formed in bcc-SLs overlying on crystalline TILs (x < 30 and x > 70). Correlation between the angular distribution of (002) crystal orientation of bcc-SL evaluated by full width at half maximum of (002) diffraction (FWHM) and a grain diameter of bcc-SL indicated that while the development of the lateral growth for bcc-SL grain reduces FWHM, crystallization of amorphous TILs hinders FWHM. $L1_0$ FePt-C granular films were fabricated under the substrate heating process over $600^{\circ}C$ with having different FWHM of bcc-SL. Hysteresis loops showed that squareness ($M_r/M_s$) of the films increased from 0.87 to 0.95 when FWHM of bcc-SL decreased from $13.7^{\circ}$ to $3.8^{\circ}$. It is suggested that the reduction of (002) FWHM affects to the overlying MgO film as well as FePt-C granular film by means of the hetero-epitaxial growth.

• Health Policy and Management
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• v.25 no.3
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• pp.240-251
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• 2015

Background: Dentist's income is quite variable. We investigate the factors underlying the distribution of dental revenue and dentist income. Methods: Financial and structural variables of private dental practices(N=13,967) were examined with 2010 Economic Census microdata which include non-insurance revenue. We conducted quantile regression method(QRM) and ordinary least square(OLS) in treating skewness and heteroskedasticity of distributions. The effective estimation for the upper and lower range of distribution becomes possible by QRM. Results: Mid-career dentists are shown to have higher revenue and income. Male dentists achieve the higher revenue and income than female dentists in all quantiles. Group practices show lower income per owner than solo practices significantly. The revenue and income are increased with increasing size of clinics. The high cost in renting the clinic office is found to have a big positive effect on the revenue but a little positive effect on the income. Interestingly the density of dentists shows negative effect on the lowest quantile of the revenue but positive effect on the highest quantile. The lowest quantile of the revenue in the capital areas have the relatively high revenue. The lowest quantile of the income in metropolitan city show higher income than those in other areas significantly. Conclusion: The suggested QRM is shown to have more effective and efficient tool in finding out determinants of dentists' revenue and income of our concern. The results of this study are expected to be employed for dentists preparing for the opening practices in their organizational settings and locational selections. The distributional efficiency of dental human resources could be accomplished if policy makers guide dentists with this knowledge.

• Journal of Ginseng Research
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• v.45 no.6
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• pp.734-743
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• 2021

Background: The underlying mechanisms of the potential tumor-suppressive effects of ginsenoside Rh2 are complex. N6-methyladenosine (m6A) RNA methylation is usually dysregulated in cancer. This study explored the regulatory effect of ginsenoside Rh2 on m6A RNA methylation in cancer. Methods: m6A RNA quantification and gene-specific m6A RIP-qPCR assays were applied to assess total and gene-specific m6A RNA levels. Co-immunoprecipitation, fractionation western blotting, and immunofluorescence staining were performed to detect protein interactions and distribution. QRT-PCR, dual-luciferase, and ChIP-qPCR assays were conducted to check the transcriptional regulation. Results: Ginsenoside Rh2 reduces m6A RNA methylation and KIF26B expression in a dose-dependent manner in some cancers. KIF26B interacts with ZC3H13 and CBLL1 in the cytoplasm of cancer cells and enhances their nuclear distribution. KIF26B inhibition reduces m6A RNA methylation level in cancer cells. SRF bound to the KIF26B promoter and activated its transcription. SRF mRNA m6A abundance significantly decreased upon KIF26B silencing. SRF knockdown suppressed cancer cell proliferation and growth both in vitro and in vivo, the effect of which was partly rescued by KIF26B overexpression. Conclusion: ginsenoside Rh2 reduces m6A RNA methylation via downregulating KIF26B expression in some cancer cells. KIF26B elevates m6A RNA methylation via enhancing ZC3H13/CBLL1 nuclear localization. KIF26B-SRF forms a positive feedback loop facilitating tumor growth.

• Wind and Structures
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• v.35 no.1
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• pp.21-34
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• 2022

Gust factor is an important parameter for the conversion between peak gust wind and mean wind speed used for the structural design and wind-related hazard mitigation. The gust factor of typhoon wind is observed to show a significant dispersion and some differences with large-scale weather systems, e.g., monsoons and extratropical cyclones. In this study, insitu measurement data captured by 13 meteorological towers during a strong typhoon Morakot are collected to investigate the statistical characteristics, height and wind speed dependency of the gust factor. Onshore off-sea and off-land winds are comparatively studied, respectively to characterize the underlying terrain effects on the gust factor. The theoretical method of peak factor based on Gaussian assumption is then introduced to compare the gust factor profiles observed in this study and given in some building codes and standards. The results show that the probability distributions of gust factor for both off-sea winds and off-land winds can be well described using the generalized extreme value (GEV) distribution model. Compared with the off-land winds, the off-sea gust factors are relatively smaller, and the probability distribution is more leptokurtic with longer tails. With the increase of height, especially for off-sea winds, the probability distributions of gust factor are more peaked and right-tailed. The scatters of gust factor decrease with the mean wind speed and height. AS/NZ's suggestions are nearly parallel with the measured gust factor profiles below 80m, while the fitting curve of off-sea data below 120m is more similar to AIJ, ASCE and EU.

• Journal of English Language & Literature
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• v.56 no.5
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• pp.851-870
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• 2010

The purpose of this paper is to investigate the distribution of /ju/ in British English, especially after the coronal sonorants /n, l, /r/. The sequence /ju/ is related with vowels such as /u/, /ʊ/, and /ʊ/, and has occasioned a variety of conflicting analyses or suggestions. One of those is in which context /j/ is deleted if we suppose that the underlying form is /ju/. The context differs according to the dialect we deal with. In British English, it is known that /j/ is deleted always after /r/, and usually after /l/ when it occurs in an unstressed word-medial syllable. To check this well-known fact I searched OED Online (the 2nd Edition, 1989) for those words which contain /n, l, r/ + /ju, jʊ, u, ʊ, (j)u, (j)ʊ/ in their pronunciations, using the search engine provided by OED Online. After removing some unnecessary words, I classified the collected words into several groups according to the preceding sonorant consonants, the positions, and the presence (or absence) of the stress, of the syllable where /ju/ occurs. The results are as follows: 1) the deletion of /j/ depends on the sonorant consonant which /ju/ follows, the position where it occurs, and the presence of the stress which /ju/ bears; 2) though the influence of the sonorant consonants is strong, the position and stress also have non-trivial effect on the deletion of /j/, that is, the word-initial syllable and the stressed syllable prefer the deletion of /j/, and word-medial and unstressed syllable usually retain /j/; 3) the stress and position factors play their own roles even in the context where the effect of /n, l, r/ is dominant.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.9
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• pp.1284-1291
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• 2002

Combustion instabilities are an important concern associated with lean premixed combustion. Laboratory-scale dump combustor was used to understand the underlying mechanisms causing combustion instabilities. Experiments were conducted at atmospheric pressure and sound level meter was used to track the pressure fluctuations inside the combustor. Instability maps and phase-resolved OH chemiluminescence images were obtained at several conditions to investigate the mechanism of combustion instability and relations between pressure wave and heat release rate. It showed that combustion instability was susceptible to occur at higher value of equivalence ratio (>0.6) as the mean velocity was decreased. Instabilities exhibited a longitudinal mode with a dominant frequency of ∼341.8 Hz, which corresponded to a quarter wave mode of combustor. Heat release and pressure waves were in-phase when instabilities occurred. Rayleigh index distribution gave a hint about the location where the strong coherence of pressure and heat release existed. These results also give an insight to the control scheme of combustion instabilities. Emission test revealed that NOx emissions were affected by not only equivalence ratio but also combustion instability.

• Asian Journal of Business Environment
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• v.9 no.2
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• pp.17-25
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• 2019

Purpose - This study uses the 'Galapagos Effect' theory proposed by Japanese researchers to investigate if there is a basis for applying the theory to South Korea, specifically in the High-Tech Service area. Research Design, Data, and Methodology - The underlying characteristics of Japanese business environment that led to the Galapagos Effect are identified, and then Korea is investigated to see if these characteristics are also evident. The case studies of three South Korean companies are explored to see if they can be considered as examples of the Galapagos Effect Results - The findings illustrate that some evidence for the existence of the Galapagos Effect does exist in Korean companies, but more research is required to systemize the phenomenon. Conclusions - The study concludes that proactive measures at a Government and business level should be implemented to mitigate the effects of the Galapagos Effect in Korea. The existence of evidence suggests that there may be grounds for more comprehensive studies in order to try and make a generalization. In addition, more study needs to be done to establish exactly which kinds of high-tech service companies are most likely to hit barriers due to the Galapagos Effect as it is not clear from the existing evidence.

• The Journal of Asian Finance, Economics and Business
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• v.6 no.3
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• pp.235-245
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• 2019

This research focuses on the relationships among customer satisfaction, delight, and loyalty. Although customer delight is one facet of an affective evaluation that can be predicted from customer satisfaction as cognitive component of the evaluation, there is no empirical examination on the casual relationship among customer satisfaction, delight, and loyalty. This study aims at addressing this gap in the service literature. The research questions are (1) How is customer satisfaction related to customer delight? and (2) Does customer delight matter in the relationship between customer satisfaction and loyalty? Data from a survey of consumers across upscale restaurant and retail bank in Korea were obtained. Our results show that customer satisfaction contributes positively to customer delight, and that customer delight plays a significant role in the relationship between customer satisfaction and loyalty. This chained relationship from customer satisfaction to customer delight to customer loyalty suggests that achieving customer delight represents one of the underlying pathways through which basic or core requirements expected by customers are satisfied. Our finding suggests that service firms need to monitor and manage their levels of customer delight as a performance metric, and delighting customers may be an important strategy to build competitive advantage through customer loyalty.

• Proceedings of the Society of Korea Industrial and System Engineering Conference
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• 2002.05a
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• pp.387-396
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• 2002

Batch processes are a significant class of processes in the process industry and play an important role in the production of high quality speciality materials. Examples include the production of semiconductors, chemicals, pharmaceuticals, and biochemicals. With on-line sensors connected to most batch processes, massive amounts of data are being collected routinely during the batch on easily measured process variables such as temperatures, pressures, and flowrates. In this paper, multivariate SPC charts for on-line monitoring of the progress of new batches are developed which utilize the information in the on-line measurements in real-time. We propose the formation of statistical model which describes the normal operation of a batch at each time interval during the batch operation. An on-line monitoring scheme based on the proposed method can handle both cross-correlation among process variables at any one time and auto-correlation over time. And the control limits for the monitoring charts are established from sound statistical framework unlike previous researches which use the external reference distribution. The proposed charts perform real-time, on-line monitoring to ensure that the batch is progressing in a manner that will lead to a high-quality product or to detect and indicate faults that can be corrected prior to completion of the batch. This approach is capable of tracking the progress of new batch runs, identifying the time periods in which the fault occurred and detecting underlying cause.