• Biomolecules & Therapeutics
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• 제6권1호
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• pp.56-62
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• 1998

Mutagenicity of recombinant human erythropoietin (rhEPO) was examined in the reverse mutation test on bacteria, in the chromosomal aberration test on cultured mammalian cells and in the micronucleus test on mice. The reverse mutation test was performed by a plate incorporation method with or wothout a metabolic activation system (59 Mix) using Salmonella typhimurium strain TA100, TA1535, TA98 and TA 1537. The rhEPO did not significantly increase revertant colonies in any of the test strains under any conditions at dose levels ranging from 1000 H/ml to 62.5 lu/plate, compared with the vehicle control. In the chromosomal aberration test using cultured Chinese Hamster Lung (CHL) cells, the number of aberrant cells was not increased in the presence or absence of 59 Mix at concentrations of 1000 lU/ml to 250 lU/ml, compared with the vehicle control. In the micronucleus test, male ICR mice were given rhEPO intraperitoneally at a dose level of 25000, 12500 and 6250 lU/kg. The incidence of bone marrow micronucleated polychromatic erythrocytes was not different from that of the vehicle control. From these results, rhEPO is considered to be non-mutagenic under the present test conditions.

• Journal of Advanced Marine Engineering and Technology
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• 제12권3호
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• pp.57-67
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• 1988

Although handicapped by the inability to bridge the size gap between small laboratory sample and large engineering component, the V charpy test sample method does possess certain advantages, such as each of preparation, simplicity of test method, speed, low cost in test machinery, and low cost per test. On the other hand, the COD test method does posses advantages, which reduce the size gap between the laboratory sample and actual engineering component. Consequently, the correlation between V charpy absorbed energy and the critical COD value is required for estimating critical COD value from the simple V charpy test results. In this paper, the high tensile strength steel AH36 plate specimens having a single edge cracked notch were investigated to find out the correlation between V charpy absorbed energy and critical COD value in the welded parts under such various welding methods as shielded metal arc welding, the submerged arc welding and the electro gas welding by means of V charpy impact test and static 3-point bending test. Main results obtained are as follow ; 1. The relationships between V charpy absorbed energy Wc' and critical COD value ($\delta_c$)show; $\delta_c$=0.0065 Wc'+0.1906. 2. Ductile- brittle transition behaviours can be estimated by means of fracture appearance and general yielding behaviours. 3. The V charpy absorbed energy of SMAW is higher than that of SAW, EGW and similar relationships are obtained in the COD tests.

• Biomolecules & Therapeutics
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• 제4권3호
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• pp.224-231
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• 1996

DA-5018, a non-narcotic analgesic agent, was examined for mutagenicity in the reverse mutation test on bacteria, chromosomal aberration test on cultured mammalian cells and micronucleus test on mice. The reverse mutation test was performed by a plate incorporation method with or without a metabolic activation system(S9 mix) using Salmonella typhimurium strain TA100, TA1535, TA98 and TA1537. DA-5018 did not significantly increase revertant colonies in any of the test strains under any conditions at concentrations ranging from 0.0049 to 1.25 mg/plate, compared with the vehicle control. In the chromosomal aberration test using cultured Chinese Hamster Lung(CHL) cells, DA-5018 did not increase the number of aberrant cells in the presence or absence of S9 mix at concentrations of 0.016 mM/plate to 0.25 mM/plate, compared with the vehicle control. In the micronucleus test, male ICR mice were given DA-5018 intraperitoneally at a dose level of 0.55, 1.10 and 2.20 mg/kg. The incidence of bone marrow micronucleated polychromatic erythrocytes in the DA-5018 treated mice was not significantly different from that of the vehicle control. These results indicate that DA-5018 does not have mutagenic potential under the present test conditions.

• Biomolecules & Therapeutics
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• 제3권4호
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• pp.294-300
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• 1995

DA-3002, an authentic recombinant human growth hormone(rhGH), was examined for mutagenicity in the reverse mutation test on bacteria, in the chromosomal aberration test on cultured mammalian cells and in the micronucleous test on mice. The reverse mutation test was performed by a plate incorporation method with or without a metabolic activation system(S9 Mix) using Salmonella typhimurium strain TA100, TA1535, TA98 and TA1537. DA-3002 did not significantly increase revertant colonies in any of the test strains under any conditions at dose levels ranging from 0.0125 to 0.4 IU/plate, compared with the vehicle control. In the chromosomal aberration test using cultured Chinese hamster lung(CHL) cells, DA-3002 did not increase the number of aberrant cells in the presence or absence of S9 mix at concentrations of 0.0125 IU/mι to 0.4 IU/mι, compared with the vehicle control. In the micronucleus test, male ICR mice were given DA-3002 intraperitoneally at a dose level of 20, 40 and 80 lU/kg. The incidence of bone marrow micronucleated polychromatic erythrocytes in the DA-3002 treated mice did not differ from that of the vehicle control. These results indicate that DA-3002 doesn't have mutagenic potential under the present test conditions.

• 한국건축시공학회지
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• 제5권3호
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• pp.91-99
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• 2005

It is required to study systematical on the concrete quality management to extend structure life because rebuilding effect is reducing under present condition of large sized and high stories structure. Concrete, which shows its intensity by hydrating action and a big change in quality according to hot weather and temperature, produces a lot of qualify problem under hot and cold weather Because of each specification and construction plan which does not have basic standard on site, concrete's quality is irregular and makes some defect. As a result, Daegu is turned out to be the longest area after investigating application period and days focused on 8 cities weather information about relationship between hot weather circumstance and construction environment. Therefore, we first surveyed the concrete material in the region and found out the problem of quality nanagement. Then figure out the way of solution. Moreover, we integrated concrete material quality management, which is applied differently to each site, to have equal quality and to reduce defect from construction site. And then, based on various test of construction condition and analysis of quality management item, we suggest effective concrete quality management to make concrete material construction standard guide and plan under hot weather.

• Composites Research
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• 제30권6호
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• pp.399-405
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• 2017

ILC 기술을 이용하여 장섬유강화 플라스틱 자동차 언더커버 시제품을 제작하고, 시제품의 성형성, 기계적성질 및 내구성을 평가하였다. 언더커버에 대한 사출성형해석과 경량화를 고려하여 시제품을 위한 사출금형을 제작하였다. 보강섬유의 잔존길이 및 분포도를 평가하여 시제품의 성형성을 검토하였고 굴곡강성 및 강도, 충격강도 등의 기계적 성질도 조사하였다. 자동차 내장부품 내구수명 평가 방법인 KLT(Key Life Test)법으로 내구성을 평가하였다.

• 한국압력기기공학회 논문집
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• 제13권1호
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• pp.75-83
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• 2017

This study investigated deformation behavior of major nuclear structural materials under cyclic loading conditions via cyclic stress-strain test. The cyclic stress-strain tests were conducted on SA312 TP316 stainless steel and SA508 Gr.3 Cl.1 low-alloy steel, which are used as materials for primary piping and reactor pressure vessel nozzle respectively, under cyclic load with constant strain amplitude and constant load amplitude at room temperature (RT) and $316^{\circ}C$. From the results of tests, the cyclic hardening and softening behavior, stabilized cyclic stress-strain behavior, and ratcheting behavior of both materials were investigated at both RT and $316^{\circ}C$. In addition, appropriate considerations for cyclic deformation behavior in the structural integrity evaluation of major nuclear components under excessive seismic condition were discussed.

• Structural Engineering and Mechanics
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• 제41권1호
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• pp.43-65
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• 2012

The primary objective of this study was to identify concrete contribution to the initial shear strength of reinforced concrete (RC) hollow columns under lateral loading. Seven large-scale RC rectangular hollow column specimens were tested under monotonic or cyclic lateral loads. The most important design parameter was column length-to-depth aspect ratio ranging between 1.5 and 3.0, and the other test variables included web area ratio, hollow section ratio, and loading history. The tests showed that the initial shear strength reduced in a linear pattern as the column aspect ratio increased, and one specimen tested under cyclic loading achieved approximately 83% of the shear strength of the companion specimen under monotonic loading. Also, several pioneering shear models proposed around the world, all of which were mainly based on tests for columns with solid sections, were reviewed and compared with the test results of this study, for their possible applications to columns with hollow sections. After all, an empirical equation was proposed for concrete contribution to the initial shear strength of RC hollow columns based on fundamental mechanics and the test results.

• Geomechanics and Engineering
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• 제18권6호
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• pp.567-574
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• 2019

In order to study the effect of stress and water pressure on the permeability of fractured rock mass under three-dimensional stress conditions, a single fracture triaxial stress-seepage coupling model was established; By using the stress-seepage coupling true triaxial test system, large-scale rock specimens were taken as the research object to carry out the coupling test of stress and seepage, the fitting formula of permeability coefficient was obtained. The influence of three-dimensional stress and water pressure on the permeability coefficient of fractured rock mass was discussed. The results show that the three-dimensional stress and water pressure have a significant effect on the fracture permeability coefficient, showing a negative exponential relationship. Under certain water pressure conditions, the permeability coefficient decreases with the increase of the three-dimensional stress, and the normal principal stress plays a dominant role in the permeability. Under certain stress conditions, the permeability coefficient increases when the water pressure increases. Further analysis shows that when the gob floor rock mass is changed from high stress to unloading state, the seepage characteristics of the cracked channels will be evidently strengthened.

• Structural Engineering and Mechanics
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• 제77권4호
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• pp.559-569
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• 2021

This study was conducted to investigate the residual bearing capacity of steel-concrete composite beams under high-cycle fatigue loading through experiments and theoretical analysis. Six test beams with stud connectors were designed and fabricated for static, complete fatigue, and partial fatigue tests. The failure modes and the degradation of several mechanical performance indicators of the composite beams under high-cycle fatigue loading were analyzed. A calculation method for the residual bearing capacity of the composite beams after certain quantities of cyclic loading cycles was established by introducing nonlinear fatigue damage models for concrete, steel beam, and shear connectors beginning with the material residual strength attenuation process. The results show that the failure mode of the composite beams under the given fatigue load appears to be primarily affected by the number of cycles. As the number of fatigue loadings increases, the failure mode transforms from mid-span concrete crushing to stud cutting. The bearing capacity of a 3.0-m span composite beam after two million fatigue cycles is degraded by 30.7% due to premature failure of the stud. The calculated values of the residual bearing capacity method of the composite beam established in this paper agree well with the test values, which indicates that the model is feasibly applicable.