• Korean journal of food and cookery science
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• v.28 no.6
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• pp.655-663
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• 2012

This study was carried out response surface analysis for brown sauce optimization. Three factors of heating time (10, 20, 30, 40, and 50 min), the added gelatin content (0, 4, 8, 12, and 16%) and the added tomato paste amount (0, 6, 12, 18, and 24%) were encoded into 5 levels (-2, -1, 0, 1, 2). After the central synthesis plan was set up to produce samples in 16 pre-arranged conditions and the sensory tests were conducted, the investigation on optimum level of these factors was conducted. The scope of optimum conditions for extraction was established through reading the range of reaction surfaces superimposed between those representing sensory properties such as color, flavor, taste, viscosity and overall acceptability was established. In this study, it was found out that the scope of optimum conditions for brown sauce extraction was 30 min for heating time, 9.00% for gelatin content and 11.25% for tomato paste amount. The reliability test proved the mentioned scope to have a similar value to that of the estimated scope when compared to the experimental values which was observed through experiments conducted under the same conditions as applied to values predicted through RSM program, enabling the verification of the reliability of derived regression formula.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.354-361
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• 2005

Vibrio fluvialis, an enteropathogenic bacterium, produces a phospholipase which is thought to be an important factor in the pathogenesis of disease. In this study, the phospholipase gene (vfp) was identified from V fluvialis (KCTC 2473) and its sequence was determined. The entire open reading frame was composed of 1,689 nuc1eotides and 563 amino acids. The phospholipase gene (vfp) was overexpressed in Escherichia coli as a his-tag fused protein. This recombinant protein (rVFP58) was solubilized with 6 M urea and purified by Ni-NTA affinity chromatography. The action mode of rVFP58 was determined by TLC and GC-MS, and it showed phospholipase B activity, which had both phospholipase A and lysophospholipase activities. The rVFP58 showed a maximum activity at pH around 9- 10 and temperature of about 40OC, and it was stable under alkaline condition over pH 9. The cytotoxicity of rVFP58 was evaluated, using a fish cell line, CHSE-2l4, and was found to cause significant cell death after 14 h of exposure to 250 $\mu$g of the protein.

• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.529-533
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• 2001

The ${\beta}$-Amylase cDNA fragment from the oomcete Saprolegnia parasitica was cloned by reverse transcription-polymerase chain reaction (RT-PCR) using degenerate oligonucleotide primers derived from conserved ${\beta}$-amylase sequences. The 5'and 3'regions of the $\beta$-amylase gene were amplified using the rapid amplification of cDNA ends (rACE) system. It consisted of an open reading frame of 1,350 bp for a protein of 450 amino acids. Comparison between the genomic and cDNA sequences revealed that the intron was not present in the coding region. The deduced amino acid sequence of the ${\beta}$-amylase gene had a 97% similarity to the ${\beta}$-amylase of Saprolegnia ferax, followed by 41% similarity to those of Arabidopsis thaliana, Hordeum vulgare, and Zea mays. The ${\beta}$-amylase gene was also expressed in Saccharomyces cerevisiae by placing it under the control of the alcohol dehydrogenase gene (ADC1) promoter.

• Journal of the Korean housing association
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• v.20 no.2
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• pp.1-10
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• 2009

The supply of digital homes has been increasing but digital home services which meet consumer needs under the various conditions have not identified. The purpose of the study was to investigate the residents' personal characteristics living in digital apartments with comparison of their trait of innovativeness toward technology. Residents who lived in two apartment complexes equipped with fundamental digital home services (Level 1 service) were selected to conduct the survey. A total of 500 responses were used for data analyses. Responses were classified into three groups based on their innovativeness toward technology for data analyses. The results of the study showed that residents' sociodemographic and housing related characteristics differed according to their acceptance trait of new technology. Early adopters and early majority were younger, higher income, and more educated than late adopters. Also, they were more likely to be renters and consider installation of digital home services before they moved in. Respondents who had higher innovativeness tended to experience more positive life changes after using digital home services and possessed more and many digital appliances. Remote control and remote meter reading service were the most preferable features among digital home services without no differences in three groups.

• The Journal of Korean Academic Society of Nursing Education
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• v.7 no.2
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• pp.379-390
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• 2001

The purpose of this study was to examine the actual condition of the adolescent status delinquency and to predict that family environments, the mother's rear attitudes and personal factors influence on the adolescent status delinquency among high school students in Busan(N=404). Under these purposes of the study, the preliminary study was performed to divided the criminal delinquency from the status delinquency, and on the basis of the result of the preliminary study, the analysis of causal pathway were performed. The data were surveyed using paper and pencil self-rating. Descriptive statistics and pathway analyses were conducted to answer research objectives. The result of this study was as follows. First, the status delinquency and the criminal delinquency are different dimensions with each other independently. Second, Contents of the status delinquencies were Drinking, Smoking, Cheating examination, Stopping out, Reckless running, Gang fighting, Reading lewdness and Watching lewd video, Sexual intercourse with the other gender, etc., The criminal delinquencies were Robbing of bicycle or auto-bicycle, Taking nalcotic drug, Robbing money of drunkard and women, Rape, Setting on fire, etc. Third, The factors of structural environments of the family and the factors of mother's rear attitude influenced on the status delinquency via adolescence's compulsivity. that is to say, if the mother's rear attitude is affective, it restrains the impulsivity of adolescence causing to the restraint of the status delinquency.

• Electrical & Electronic Materials
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• v.8 no.2
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• pp.229-236
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• 1995

Multimedia has created a system environment that needs a combination of diverse peripherals, faster I/O, and easier configuration. The sheer volume of data one can expect with multimedia hardware and applications storage systems of higher capacity and faster data transfer rate. Unlike the magneto-optical(MO)disk technology which uses bias magnetic field in writing, both the reading and the writing in the phase change (PC) technology are performed only by laser light. In PC optical media, an active layer is reversibly converted between amorphous state and crystalline state by changing irradiation conditions of focused laser beam. Thus, as compared with MO disk, the PC disk has such great advantages that signals can be reproduced by change of reflectance of laser beams in the same manner as the compact disc. The reflectivity of a phase-change spot can be altered in a single pass under the head only through modulation of laser power. The principles and the current status of phase-change optical recording media combined with possible applications are discussed in the present article.

• Journal of Fisheries and Marine Sciences Education
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• v.25 no.1
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• pp.198-210
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• 2013

The purpose of this study investigates high school students' recognition and realities on the integrated science essay and is to suggest desirable direction of integrated science essay of how eduction. To this end, this paper was a questionnaire developed for use, it consists of the status, the writing skills and recognition of integrated science essay. Firstly, all grade students recognize the interest in integrated science essay class, but the need for third grade boys urgently was feeling. Second, STEAM class as a whole than average preference was. Third, integrated science essay was the most relevant, then was mathematics, languages, philosophy ethics, and social. Fourth, integrated science essay class with boys than girls in grade 1, science essay writing, reading science-related essay books, grammar, knowledge of the science and philosophy of science lessons, classes STEAM, read commentary essay reference all on the item, the higher affinity. Currently being implemented in integrated science essay test compared to the first, team teaching approach in schools project under one class teaches students how many teachers should be made. Second, it would require modifications of course content tailored to the preferences of female preference for science higher grade female students to disappear.

• KEPCO Journal on Electric Power and Energy
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• v.2 no.2
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• pp.245-251
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• 2016

Despite the exponential throughput improvement in mobile communications systems, their ability to satisfy requirements of state-of-the-art and future applications of advanced metering infrastructure (AMI) is still under investigation. Challenges are mainly due to the inadequacy of third generation partnership project (3GPP) networks to support large amounts of devices simultaneously, while the number of AMI end-devices and the frequency of their data transmission increase with new AMI-based applications. In this introductory survey, innovative and future AMI applications and their communication requirements are first reviewed. Then, we identify challenges of 3GPP long term evolution (LTE) in enabling future AMI applications. More importantly, the latest improvements to LTE-A standard release 12 and 13 are reviewed and analyzed with regards to their potential to improve the quality of LTE-enabled AMI. It is found that 3GPP enhancements on machine type communications (MTC) standards will significantly enhance AMI communications. Beyond MTC specifications, non-MTC-specific enhancements such as carrier aggregation and multi-connectivity for user equipment will also contribute greatly to improving reliability and availability of AMI devices. The paper's focus is towards improved backhaul support for innovative and future AMI applications, beyond traditional automatic meter reading.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.263-269
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• 2016

Temperate phages have been suggested to carry virulence factors and other lysogenic conversion genes that play important roles in pathogenicity. In this study, phage TEM123 in wild-type Staphylococcus aureus from food sources was analyzed with respect to its morphology, genome sequence, and antibiotic resistance conversion ability. Phage TEM123 from a mitomycin C-induced lysate of S. aureus was isolated from foods. Morphological analysis under a transmission electron microscope revealed that it belonged to the family Siphoviridae. The genome of phage TEM123 consisted of a double-stranded DNA of 43,786 bp with a G+C content of 34.06%. A bioinformatics analysis of the phage genome identified 43 putative open reading frames (ORFs). ORF1 encoded a protein that was nearly identical to the metallo-β-lactamase enzymes that degrade β-lactam antibiotics. After transduction to S. aureus with phage TEM123, the metallo-β-lactamase gene was confirmed in the transductant by PCR and sequencing analyses. In a β-lactam antibiotic susceptibility test, the transductant was more highly resistant to β-lactam antibiotics than S. aureus S133. Phage TEM123 might play a role in the transfer of β-lactam antibiotic resistance determinants in S. aureus. Therefore, we suggest that the prophage of S. aureus with its exotoxin is a risk factor for food safety in the food chain through lateral gene transfer.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.10a
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• pp.893-903
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• 2008

A method for predicting surface failures which occur during heavy rainfall on mountain slopes is proposed by using the digital land form model that is obtained by reading altitude on a topographical map at 10m grid point space. A depth of a potential failure layer is assumed at each grid point. In the layer, an infiltrated water movement from cell to cell is modeled in the study (cell is a square of the grid). Infiltrated ground water levels which show the three dimensional effects of a topographical factor in an area can be hourly calculated at every cell by the model. The safety factor of every cell is also calculated every hour by the infinite slope stability analysis method with the obtained infiltrated ground water level. Failure potential delineation is defined here as the time when the safety factor becomes less than unity under the assumptions that effective rainfall is 20mm/h and continues 20 hours.