• FOCUS: LIFE SCIENCE
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• no.1
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• pp.1.1-1.4
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• 2024

This article provides comprehensive guidance on the maintenance, cleaning, regeneration, and storage of silica-based HPLC (High-Performance Liquid Chromatography) columns. The general considerations emphasize the importance of using in-line filters and guard cartridges to protect columns from blockage and irreversible sample adsorption. While these measures help, contamination by strongly adsorbed sample components can still occur over time, leading to an increase in back pressure, loss of efficiency, and other issues. To maximize column lifetime, especially with UHPLC (Ultra-High Performance Liquid Chromatography) columns, it is advisable to use ultra-pure solvents, freshly prepared aqueous mobile phases, and to filter all samples, standards, and mobile phases. Additionally, an in-line filter system and sample clean-up on dirty samples are recommended. However, in cases of irreversible compound adsorption or column voiding, regeneration may not be possible. The document also provides specific recommendations for column cleaning procedures, including the flushing procedures for various types of columns such as reversed phase, unbonded silica, bonded normal phase, anion exchange, cation exchange, and size exclusion columns for proteins. The flushing procedures involve using specific solvents in a series to clean and regenerate the columns. It is emphasized that the flow rate during flushing should not exceed the specified limit for the particular column, and the last solvent used should be compatible with the mobile phase. Furthermore, the article outlines the storage conditions for silica based HPLC columns, highlighting the impact of storage conditions on the column's lifetime. It is recommended to flush all buffers, salts, and ion-pairing reagents from the column before storage. The storage solvent should ideally match the one used in the initial column test chromatogram provided by the manufacturer, and column end plugs should be fitted to prevent solvent evaporation and drying out of the packing bed.

• Journal of the Earthquake Engineering Society of Korea
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• v.28 no.2
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• pp.85-92
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• 2024

This study presents code-compliant seismic details by addressing dry mechanical splices for precast concrete (PC) beam-column connections in the ACI 318-19 code. To this end, critical observations of previous test results on precast beam-column connection specimens with the proposed seismic detail are briefly reported in this study, along with a typical reinforced concrete (RC) monolithic connection. On this basis, nonlinear dynamic models were developed to verify seismic responses of the PC emulative moment-resisting frame systems. As the current design code allows only the emulative design approach, this study aims at identifying the seismic performances of PC moment frame systems depending on their emulative levels, for which two extreme cases were intentionally chosen as the non-emulative (unbonded self-centering with marginal energy dissipation) and fully-emulative connection details. Their corresponding hysteresis models were set by using commercial finite element analysis software. According to the current seismic design provisions, a typical five-story building was designed as a target PC building. Subsequently, nonlinear dynamic time history analyses were performed with seven ground motions to investigate the impact of emulation level or hysteresis models (i.e., energy dissipation performance) on system responses between the emulative and non-emulative PC moment frames. The analytical results showed that both the base shear and story drift ratio were substantially reduced in the emulative system compared to that of the non-emulative one, and it indicates the importance of the code-compliant (i.e., emulative) connection details on the seismic performance of the precast building.

• Journal of the Korea Concrete Institute
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• v.26 no.4
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• pp.429-440
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• 2014

Since the World Trade Center and Pentagon attacks in 2001, terror, military attack, or man-made disaster caused impact, explosion, and fire accident have frequently occured on civil infrastructures. However, structural behavior researches on major Prestressed Concrete (PSC) infrastructures such as bridges, tunnels, Prestressed Concrete Containment Vessel (PCCVs), and LNG tanks under extreme loading are significantly lacking. Especially, researches on possible secondary fire scenarios after terror, bombing, collision of vehicles and vessels on concrete structures have not been performed domestically where most of the past researches related to extreme loadings on structures focused on an independent isolated extreme loading scenario. Due to the outcry of public concerns and anxiety of potential terrorist attacks on major infrastructures and structures, a study is urgently needed at this time. Therefore, in this study, the bi-directional prestressed concrete $1400{\times}1000{\times}300mm$ panels applied with 430 kN prestressing force using unbonded prestressing thread bars were experimentally evaluated under impact, fire, and impact-fire combined loadings. Due to test site restrictions, impact tests were performed with 14 kN impactor with drop heights of 10m and 3.5 m to evaluate impact resistance capacity. Also, fire and impact-fire combined loading were tested using RABT fire loading curve. The measured residual strength capacities of PSC and RC specimens applied with impact, fire, impact-fire combined loadings were compared with the residual strength capacity of undamaged PSC and RC specimens for evaluation. The study results can be used as basic research data for related research areas such as protective design and numerical simulation under extreme loading scenarios.

• Structural Engineering and Mechanics
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• v.63 no.3
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• pp.361-370
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• 2017

Through the use of finite element analysis and acoustic emission techniques we have evaluated the interfacial failure of a carbon fiber reinforced polymer (CFRP) repair patch on a notched aluminum substrate. The repair of cracks is a very common and widely used practice in the aeronautics field to extend the life of cracked sheet metal panels. The process consists of adhesively bonding a patch that encompasses the notched site to provide additional strength, thereby increasing life and avoiding costly replacements. The mechanical strength of the bonded joint relies mainly on the bonding of the adhesive to the plate and patch stiffness. Stress concentrations at crack tips promote disbonding of the composite patch from the substrate, consequently reducing the bonded area, which makes this a critical aspect of repair effectiveness. In this paper we examine patch disbonding by calculating the influence of notch tip stress on disbond area and verify computational results with acoustic emission (AE) measurements obtained from specimens subjected to uniaxial tension. The FE results showed that disbonding first occurs between the patch and the substrate close to free edge of the patch followed by failure around the tip of the notch, both highest stress regions. Experimental results revealed that cement adhesion at the aluminum interface was the limiting factor in patch performance. The patch did not appear to strengthen the aluminum substrate when measured by stress-strain due to early stage disbonding. Analysis of the AE signals provided insight to the disbond locations and progression at the metal-adhesive interface. Crack growth from the notch in the aluminum was not observed until the stress reached a critical level, an instant before final fracture, which was unaffected by the patch due to early stage disbonding. The FE model was further utilized to study the effects of patch fiber orientation and increased adhesive strength. The model revealed that the effectiveness of patch repairs is strongly dependent upon the combined interactions of adhesive bond strength and fiber orientation.

• Journal of the Korea Concrete Institute
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• v.16 no.4 s.82
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• pp.451-458
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• 2004

This study describes the basic concept and the applicability of Hybrid Reinforcement System using conventional steel reinforcing bars and Fiber Reinforced Polymer bars. The concrete bridge decks are assumed to be supported by beams and reinforced with two layers of reinforcing bars. In concrete bridge deck using HRS, the top tensile force for negative moment zone on beam supports is assumed to be resisted by FRP reinforcing bars, and the bottom tensile force for positive moment zone in the middle of hem supports is assumed to be resisted by conventional steel reinforcing bars, respectively. The FRP reinforcing bars are non-corrosive. Thus, the steel reinforcement is as far away as possible from the top surface of the deck and protected from intrusion of corrosive agent. HRS concrete bridge deck has sufficient ductility at ultimate state as the following reasons; 1) FRP bars have lower elastic modulus and higher ultimate strain than steel re-bars have, 2) FRP bars have lower ultimate strain if provided higher reinforcement ratio, 3) ultimate strain of FRP bars can be reduced if FRP bars are unbonded. Test results showed that FRP and HRS concrete slabs are not failed by FRP bar rupture, but failed by concrete compression in the range of ordinary reinforcement ratio. Therefore, in continuous concrete bridge deck using HRS, steel reinforcing bars for positive moment yield and form plastic hinge first and compressive concrete fail in the bottom of supports or in the top of the middle of supports last. Thus, bridge deck consumes significant inelastic strain energy before its failure.

• Journal of the Korea Concrete Institute
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• v.19 no.6
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• pp.773-783
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• 2007

A simple unbonded-type shear strengthening technique for reinforced concrete beams using wire rope units is developed. Six two-span continuous T-beams externally strengthened with wire rope units and an unstrengthened control beam were tested. The main variables investigated were the amount and prestressing force of wire rope units. All specimens had the same geometrical dimension and arrangement of internal reinforcement. Influence of the distribution of vertical stresses in beam web owing to the prestressing force of wire rope units on the diagonal shear cracking load and the ultimate shear capacity of beams tested is presented. Based on the current study, it can be concluded that the amount and initial prestress of wire rope should be limited to be above 2.5 times the minimum shear reinforcement ratio specified in ACI 318-05 and below 0.6 times its own tensile strength, respectively, to ensure the enhancement of shear capacity and ductile failure mode of the strengthened beams. A numerical analysis based on the upper-bound theorem is developed to assess the shear capacity of continuous T-beams strengthened with wire rope units. From the comparisons of measured and predicted shear capacities, a better agreement is achieved in the proposed numerical analysis than in empirical equations recommended by ACI 318-05.

• Journal of the Korea Concrete Institute
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• v.18 no.4 s.94
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• pp.527-534
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• 2006

This paper outlines a new strengthening technique for concrete beams using externally unbended high-strength bars. The advantages of proposed method lie in speed and simplicity of construction compared to the alternative strengthening method. Externally unbended reinforcement retains many of the advantages over external unbended prestressed tendons. It eliminates time consuming stressing operations. Clearance requirements around anchorages are reduced as access is not required for prestressing jacks. Test results of eight specimens on reinforced concrete beams using different reinforcement materials such as carbon fiber sheet, steel plate and high-tension bar are reported. The beam strengthened by carbon fiber sheet showed a brittle failure mode due to the separation of fiber. As a result of draped profile of external bar, the maximum strength of the beam were increased by up to 212 percent and the deflections were reduced by up to 65 percent. Test results show that the beams reinforced with high-tension bar are superior to reference specimens, especially for the strength and deformation capacity.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.28 no.5
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• pp.535-542
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• 2015

A reactor containment acts as a final barrier to prevent leakage of radioactive material due to the possible reactor accidents into external environment. Because of the functional importance of the containment building, the SIT(Structural Integrity Test) for containments shall be performed to evaluate the structural acceptability and demonstrate the quality of construction. In this paper, numerical analyses are presented, which simulate the results obtained from the SIT for a prestressed concrete(PSC) structure. A sophisticate structural analysis model is developed to simulate the structural behavior during the SIT properly based on various preliminary analysis results considering contact condition among structural elements. From the comparison of the analysis and test results based on the acceptance criteria of ASME CC-6000, it can be concluded that the construction quality of the containment has been well maintained and the acceptable performance of new design features has been verified.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.28 no.5
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• pp.523-533
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• 2015

A reactor containment acts as a final barrier to prevent leakage of radioactive material due to the possible reactor accidents into external environment. Because of the functional importance of the containment building, the SIT(Structural Integrity Test) for containments shall be performed to evaluate the structural acceptability and demonstrate the quality of construction. An initial numerical analysis was performed to simulate the results obtained from the SIT for a prestressed concrete(PSC) structure. But the analysis results by the initial model expected smaller displacements than the measured ones by 30% at some locations. Accordingly, the research and development to improve the initial model to corelate the measured results of the SIT more properly have been performed. In this paper, the effects of the loss of concrete due to duct for tendons and the contact of duct and tendons in un-bonded tendon system are mainly evaluated based on the preliminary analysis results. In addition, the importances of the proper definition of mesh connectivity among structural elements of concrete, liner plates, rebars and tendons are discussed.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.6D
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• pp.951-960
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• 2006

Structural pavement analysis considering lateral loads of moving vehicle was carried out in order to simulate passing vehicle loads under various interface conditions. To verify of existing multi-layer elastic analysis of layer interface effect parameters, this study compared outputs by using ABAQUS, a three dimensional finite element program and KENLAYER, multi-layer elastic analysis as vertical load was applied to the surface of asphalt pavements. Pavement performance depending on interface conditions was quantitatively evaluated and fundamental study of layer interface effect parameters was performed in this study. As results of the study, if only vertical loads of moving vehicle is applied, subdivision of either fully bonded or fully unbonded is enough to indicate interface effect parameters. On the other hand, when lateral loads are applied with vertical loads, pavement behavior and performance are greatly changed with respect to layer interface conditions. The thinner thickness of the asphalt layer is and the smaller elastic moduli of the asphalt layer is, the more pavement behavior is influenced by interface conditions. In addition, regression analysis equation analytically computing tensile strain which was considered thicknesses and elastic moduli of the asphalt layer and layer interface effect parameters at the bottom of the asphalt layer was presented using database from numerical analyses on national pavement model sections.