• ALGAE
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• 제23권3호
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• pp.201-207
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• 2008

Based on samplings during 2006-2007 on the intertidal zone of Playa Tampico, Eréndira and Punta Baja, Baja California, Ulva pertusa Kjellman (Ulvales, Chlorophyta) was recorded for the first time for the Pacific coast of Mexico. An alga native to Asia, U. pertusa has just recently been recorded in the southern coast of California (USA). The identification of U. pertusa was based on the observation of the morphology and reproductive characters of the collected specimens. Furthermore, the species identity was confirmed by molecular comparison between nuclearencoded ITS2 sequences of the Mexican samples and those of other Ulva species in GenBnak data. Habitat and distribution of U. pertusa along the Pacific coast of North America are also described. Considering our new report of its occurrence in Mexico, we conclude that U. pertusa is expanding its geographical distribution by trans-ocean introductions.

• Journal of Species Research
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• 제11권2호
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• pp.79-88
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• 2022

The Ulvophyceae are a diverse group and most species are distributed in marine environments, however, a smaller diversity occurs in freshwater and terrestrial habitats. Also, the diversity of species belonging to Ulvophyceae has been superficially investigated in Korea. Here, we identify two freshwater ulvophycean species based on the morphology, ultrastructural observation, and phylogenetic analysis. As a result, their taxonomic classifications are as follows: order Ulvales Blackman and Tansley, 1902 - family Kornmanniaceae Golden and Cole, 1986 - Lithotrichon pulchrum Darienko and Proschold 2017; order Ulotrichales Borzi, 1895 - family Tupiellaceae Skaloud and Leliaert, 2018 - Tupiella speciosa Darienko and Proschold 2017. The family Tupiellaceae and the two genera Lithotrichon and Tupiella are reported for the first time in Korea.

• 한국수산과학회지
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• 제30권3호
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• pp.388-392
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• 1997

해조류중 우리나라 전연안에 가장 흔히 분포하는 갈파래목 녹조류인 참홑파래와 구멍갈파래, 참갈파래, 잎파래, 실파래, 가시파래등을 대상으로 RAPD방법을 이용한 각 지역 개체간의 유전적 유사도를 조사하였다. Total DNA는 엽체로부터 LiCl를 사용한 DNA추출방법으로 분리하였으며, 추출된 DNA는 $3ng/{\mu}\ell$되게 희석한후 in vitro에서 arbitrary primer와 함께 45회 PCR amplification시켰다. 그 결과 34종류 primer들로부터 240bp에서 1.5kb의 다양한 크기로 증폭된 1227개의 전기영동상의 band를 볼 수 있었으며, Jaccard공식에 따라 그 유사도를 구하였다. 파래목의 유전적 유사도는 $7\%$에서 $36\%$범위의 유사도를 보였다. 그중 참흩파래가 다른 갈파래과들로부터 가장 낮은 유사도값을 보여주었으며, 또한 primer OPB-01 (CATCCCCCTG)을 사용하였을때 갈파래과에서 공통적인 630bp크기의 생성물을 생산하지않는 특징을 나타내었다.

• ALGAE
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• 제22권3호
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• pp.221-228
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• 2007

Frequent occurrences of green tides caused by Ulva species (Ulvales, Ulvophyceae) associated with eutrophication along enclosed coasts are currently causing environmental problems in coastal ecosystems. In addition, increasing intercontinental introductions of coastal marine organisms, including Ulva, are also a serious issue. However, due to the considerable morphological plasticity of this genus, the taxonomy of Ulva species based on morphological studies is problematic. Therefore, in order to elucidate the species diversity and seasonal changes of the dominant Ulva species in Mikawa Bay, central Honshu, Japan, we made seasonal collections of Ulva species at seven localities, and identified the dominant species using the ITS2 rDNA region sequences. We identified the following nine taxa as common Ulva species in the area: 1) Ulva pertusa Kjellman; 2) U. ohnoi Hiraoka et Shimada; 3) U. linza L.; 4) U. californica Wille; 5) U. flexuosa Wulfen; 6) U. fasciata Delile; 7) U. compressa L.; 8) U. armoricana Dion et al.; 9) U. scandinavica Bliding. Among the species, U. pertusa was most common and dominant from spring to summer, and U. ohnoi from autumn to winter. Ulva californica and U. scandinavica have not been reported before from Japan.

• 한국해양바이오학회지
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• 제14권1호
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• pp.51-60
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• 2022

A green alga specimen was collected from the eastern coast of Korea. This species shared the typical features of genus Ulva and was characterized by irregularly shaped thalli, relatively small and thick thallus, entire undulate margins without serrations, and one or two pyrenoids per cell. In a phylogenetic tree, based on sequences of the nuclear-encoded internal transcribed spacer region, it nests as a sister clade to a few species including Ulva ohnoi, which has a relatively large thallus. This Korean algal specimen differs from the species forming the same subclades, including U. ohnoi, Ulva fasciata, Ulva reticulata, and Ulva gigantean, and has a relatively small (3-8 cm) and thick (60-100 ㎛) thallus. Of these species, U. ohnoi, originally described from Japan, is similar to the Korean alga as it had a thick thallus of 30-90 ㎛, but it has microscopic serrations on the thallus margin, unlike the Korean alga. The genetic distance between the Korean alga species and the aforementioned species was determined to be 1.8%-4.8%, indicating an inter-specific divergence level at the genus Ulva. Herein, Ulva grossa sp. nov. (Ulvales, Chlorophyta) from Korea is described based on the morphological and molecular analyses.

• 환경생물
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• 제35권3호
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• pp.329-334
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• 2017

A marine ulvalean species (Chlorophyta) was collected from Imgok, which is located on the eastern coast of Korea. This species is morphologically characterized by distromatic, filiform to strap compressed or tubular thallus. Many branches were found near the base, but lacked proliferations. Cells were longitudinally aligned in the younger part of the branch and were disordered in the older part of the branch. A cap-like parietal chloroplast with one or two pyrenoids was contained in each cell. In a phylogenetic tree based on ITS and rbcL sequences, this species was nested in the same clade as Ulva torta and U. clathratioides from Australia, but formed a sister clade to U. torta from Japan. However, the genetic divergence between them was included in an intraspecific variation range within Ulva. This finding suggests that U. clathratioides should be reduced to a synonym of U. torta. Accordingly, the Korean alga was identified as U. torta based on the morphological and molecular data. This investigation is the first record of U. torta in the Korean marine algal flora.

• 환경생물
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• 제37권3호
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• pp.293-298
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• 2019

A marine ulvalean species (Chlorophyta) was collected from the eastern coast of Korea. This species is morphologically characterized by a distromatic, dark to medium green and mostly irregularly orbicular or irregularly expanded thallus with entire or undulate margin without serrations. Vegetative cells are irregularly polygonal with distinctly rounded corners in shape, and have chloroplast completely covering the outer cell wall and one to two pyrenoids per cell. In a phylogenetic tree based on ITS (Internal Transcribed Spacer) sequences, this Korean alga nests in the same clade with Ulva sublittoralis, as a sister clade of U. californica, U. flexuosa and U. tanneri, which share the irregularly orbicular or expanded thallus normally without teeth cells. The genetic divergence between them is intraspecific within Ulva. Accordingly, it is identified as U. sublittoralis based on the morphological and molecular data. This is the first record of Ulva sublittoralis in the Korean marine algal flora.

• Journal of Plant Biology
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• 제33권2호
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• pp.141-146
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• 1990

Germination and vegetative growth of Enteromorpha multiramosa Bliding from Pyoson, Cheju Island were investigated in laboratory under various combinations of temperature (5-$25^{\circ}C$) and salinity (8-48$^{\circ}C$). Percent level of germination was relatively high at all combinations of the two factors. The highest value among the combinations was revealed at 15$^{\circ}C$ and 32$\textperthousand$. Dry weight also was fairly high at all levels of combination with maximum value at 2$0^{\circ}C$ and 32$\textperthousand$. Analysis of variance for germination and growth was completed respectively and polynomial prediction models were constructed. F ratio revealed that all factors had a significant effect (p<0.001) on percentage of germination and dry weight, and their interactions also were significant (p<0.001), although the F ratio of interactions was far less than that for either the separate effect of temperature or salinity. Response surface of polynomial equation represented that temperature influenced less than salinity on germination, while it effected remarkably on vegetative growth, so the Enteromorpha multiramosa was kept to visible macrothalli from winter to spring in Cheju Island.

• Journal of Plant Biology
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• 제35권1호
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• pp.61-67
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• 1992

환경과 관련된 해산 녹조류 잎파래(Enteromorpha linza)의 형태적 변이 양상을 파악하기 위하여 한국 전 해안에서 채집된 재료를 대상으로 각 형질을 정량화한 다음 주성분 분석을 실시하였다. 그 결과 파도 등에 노출된 개체군의 식물체는 보호된 개체군의 식물체보다 자루의 길이와 엽록체의 최대 길이가 길고 엽체의 폭은 좁은 형태적 특징을 보였다. 각 형질에 대한 개체군 간의 형태적 차이 유무를 보여주는 다중비교분석에서도 이와 같은 결과를 확인할 수 있어, 파도 등에 의한 노출 정도로 인하여 나타나는 표현형의 변이성 출현에 대한 타당성을 확인할 수 있었다.

• ALGAE
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• 제34권4호
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• pp.253-266
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• 2019

Several species classified to the genus Ulva are primarily responsible for causing green tides all over the world. For almost two decades, green tides have been resulted in numerous ecological problems along the eastern coast of Jeju Island, Korea. In order to characterize the species of Ulva responsible for causing the massive blooms on Jeju Island, we conducted DNA barcoding of tufA and rbcL sequences on 183 specimens of Ulva from eight sites on Jeju Island. The concatenated analysis identified five bloom-forming species: U. australis, U. lactuca, U. laetevirens, U. ohnoi and a novel species, U. pseudo-ohnoi sp. nov. Among them, U. australis, U. lactuca, and U. laetevirens caused to the blooms coming mainly from the substratum. U. ohnoi and U. pseudo-ohnoi sp. nov. were causative the free-floating blooms. Four species, except U. australis, are characterized by marginal teeth. A novel species, U. pseudo-ohnoi sp. nov., is clearly diverged from the U. lactuca, U. laetevirens, and U. ohnoi clade in the concatenated maximum likelihood analysis. Accurate species delimitation will contribute to a management of massive Ulva blooms based on this more comprehensive knowledge.