• Title/Summary/Keyword: Ultraviolet-B (UV-B)

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Simultaneous Determination of (+)-Pseudoephedrine and (-)-Ephedrine in Ephedra intermedia by HPLC-UV (HPLC-UV를 이용한 중마황의 (+)-Pseudoephedrine과 (-)-Ephedrine의 동시분석법 개발)

  • Jeong, Birang;Yoon, Yoosik;Shin, Soon Shik;Kwon, Yong Soo;Yang, Heejung
    • Korean Journal of Pharmacognosy
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    • v.48 no.1
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    • pp.93-96
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    • 2017
  • Ephedra alkaloids, (-)-ephedrine, (+)-pseudoephedrine, (-)-N-methylephedrine, (+)-N-methylpseudoephedrine, (-)-norephedrine and (+)-norpseudoephedrine, from ephedra herb are sympathomimetic agonists causing an increase of metabolism, blood pressure and perspiration. In this study, we developed the validation method of (+)-pseudoephedrine and (-)-ephedrine, two major ephedra alkaloids in Ephedra spp., by high-performance liquid chromatography-ultraviolet spectrometer (HPLC-UV). HPLC analysis was performed using a HECTOR-M C18 column operating at $35^{\circ}C$, and UV detection at 215nm. The mobile phase used a gradient flow with 25 mM SDS in water (A) and acetonitrile (B).

Antioxidant Activity and Whitening activity of Psidium guajava leaf extract (구아바 잎 추출물의 항산화 및 미백 활성 효과)

  • You, Seon-hee
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.2
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    • pp.296-304
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    • 2017
  • The purpose of this study was to investigate the applicability of the Psidium guajava leaf extract as a whitening functional cosmetic material. We measured DPPH radical scavenging activity, intracellular ROS, cytotoxicity in B16F10 melanoma cells and cytoprotective effect on ultraviolet A, in vitro tyrosinase inhibitory effect and melanin biosynthesis inhibitory effect. The antioxidative effect was confirmed through high DPPH radical scavenging activity and intracellular ROS activity inhibition measurement of the Psidium guajava leaf extract. The survival rate of B16F10 melanoma cells was more than 98% at all concentrations, and the cytoprotective effect from ultraviolet ray A was found to increase in a concentration-dependent manner. In addition, in vitro tyrosinase activity inhibitory effect of 10% and melanin biosynthesis inhibitory effect of 20% were observed. Through less toxicity for B16F10 melanoma cell, high antioxidant activity, inhibition of tyrosinase activity and melanin biosynthesis inhibitory effect, we confirmed the possibility of developing the Psidium guajava leaf extract as a whitening functional cosmetic material with a safe and excellent whitening effect.

Inhibitory Effects of Marine Natural Products on Melanogenesis in B16 Melanoma Cells (B16 멜라닌 세포에서 해양소재 추출물의 멜라닌 생성 저해 효과)

  • Lee, Chan;Jang, Jung-Hee;Ahn, Eun-Mi;Park, Chan-Ik
    • The Korea Journal of Herbology
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    • v.27 no.4
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    • pp.73-80
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    • 2012
  • Objectives : Under normal condition melanin protects the skin from extracellular stimuli including ultraviolet (UV)-induced oxidative skin damages, but excess production and accumulation of melanin can induce hyperpigmentation causing esthetic problems. Therefore, in this study we tried to search for natural skin whitening materials from marine natural resources. Methods : Water and ethanol extracts of marine natural resources were prepared from Porphyra thalli (PT), Laminariae thallus (LT), Ostreae concha (OC), Sargassum thallus (ST), Undaria thallus (UT), Codium thalli (CT), Enteromorpha thalli (ET), Syngnathoides biaculeatus (SB), and Hippocampus coronatus (Hc). Their effects against UVB and ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH)-induced melanogenesis were investigated based on melanin formation in B16 mouse melanoma cells. The mRNA and protein expression of enzymes involved in the melanogenic process were further examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. Results : Water extract of Ostreae concha (OCW/E) effectively inhibited UVB and ${\alpha}$-MSH-induced melanin production in B16 melanocytes, which seemed to be mediated by inhibition of mRNA expression of tyrosinase and tyrosinase-related protein 1 (TRP-1). In another experiment, ethanol extracts from Porphyra thalli (PTE/E), Laminariae thallus (LTE/E), Sargassum thallus (STE/E), Undaria thallus (UTE/E), Codium thalli (CTE/E), Syngnathoides biaculeatus (SBE/E), and Hippocampus coronatus (HcE/E) significantly suppressed UVB and ${\alpha}$-MSH-induced melanin formation. Furthermore, ethylacetate fraction isolated form LTE/E (LTE/EEt) decreased UVB and ${\alpha}$-MSH-elevated extracellular melanin levels via inhibition of tyrosinase protein expression. Conclutions : These results suggest that marine natural resources such as Porphyra thalli, Laminariae thallus, Ostreae concha, Sargassum thallus, Undaria thallus, Codium thalli, Syngnathoides biaculeatus and Hippocampus coronatus have anti-melanogenic effects, thereby exhibiting high potentials to be utilized as one of the ingredients for the development of new whitening functional cosmetics.

Highly Luminescent Aniline and TiO2 Composite: The Effect of Weight Ratio of Aniline and TiO2

  • Kim, Byoung-Ju;Park, Eun-Hye;Kang, Kwang-Sun
    • Current Photovoltaic Research
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    • v.4 no.1
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    • pp.8-11
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    • 2016
  • Strong deep ultraviolet emitting aniline and $TiO_2$ composite has been synthesized via hydrolysis and condensation reactions of titaniumisopropoxide ($Ti(OPr)_4$), aniline, and acetic anhydride. Three different weight ratios of aniline and $Ti(OPr)_4$ including 3:1 ($TiO_2An-A$), 2:1 ($TiO_2An-B$), and 1:1 ($TiO_2An-C$) were synthesized and characterized their optical properties. The FTIR spectra of the $TiO_2An-A$, -B, and -C showed the absorption intensities of the benzene ring stretching and bending vibrations, and benzene ring -CH stretching, bending, and deformation vibrations increased with the increase of the amount of aniline. The UV-visible absorption spectra showed that the UV region absorption was slightly increased with the increase of the amount of aniline. The photoluminescence (PL) intensities were exponentially increased with the increase the excitation wavelength from 307 to 317 nm, steadily increased from 300 to 313 nm and slowly increased from 302 to 308 nm for $TiO_2An-A$, -B, and -C, respectively and decreased thereafter. Therefore, the PL intensity is strongly dependent on the weight ratio of $Ti(OPr)_4$ and aniline.

Ethane Evolution in Cucumber Plants by Air Pollutants in Relation to Plants Injury (대기오염 물질 처리에 의한 오이 장해와 에탄 생성)

  • Bae, Gong-Young
    • Korean Journal of Environmental Agriculture
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    • v.17 no.2
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    • pp.127-131
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    • 1998
  • Ethane was measured to know whether active oxyzens may induce phytoxicity in stressedcucumber plant.The time course of the increase in ethane evolution was the same as that of the increase of visible injury in all treatments except $SO_2$ treatment.This result showed that air pollutants-induced plant damages were closely related to ethane evolution.And evolution of ethane was more increased in combined stress than singly one,suggesting that phytotoxicity was more severe in complex sterss.Also, evolution of ethane was enhanced in the light condition and scavengers of active oxygen were inhibited,showing that plant damage that plant damage were cause by active oxygens.

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ON THE HOMOGENEITY OF THE EXTINCTION LAW IN OUR GALAXY

  • Bondar, A.;Galazutdinov, G.;Patriarchi, P.;Krelowski, J.
    • Journal of The Korean Astronomical Society
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    • v.39 no.3
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    • pp.73-80
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    • 2006
  • We analyze the extinction law towards several B1V stars-members of our Galaxy, searching for possible discrepancies from the galactic average extinction curve. Our photometric data allow to build extinction curves in a very broad range: from extreme UV till infrared. Two-colour diagrams, based on the collected photometric data from the ANS UV satellite, published UBV measurements and on the infrared 2MASS data of the selected stars, are constructed. Slopes of the fitted straight lines are used to build the average extinction curve and to search for discrepant objects. The selected stars have also been observed spectroscopically from the Terskol and ESO Observatories; these spectra allow to check their Sp/L's. The spectra of only about 30% of the initially selected objects resemble closely that of HD144470, considered as the standard of B1 V type. Other spectra either show some emission features or belong clearly to another spectral types. They are not used to build the extinction curve. Two-colour diagrams, constructed for the selected B1 V stars, showing no emission stellar features, prove that the interstellar extinction law is homogeneous in the Galaxy. Both the shape of the curve and the total-to-selective extinction ratio do not differ from the galactic average and the canonical value(3.1) respectively. The circumstellar emissions usually cause some discrepancies from the average interstellar extinction law; the discrepancies observed in the extraterrestrial ultraviolet, usually follow some misclassifications.

Dyeing Fastness and Functional Properties of Cotton Dyed with Astringent Persimmon Juice

  • Jung, Jin-Soun
    • International journal of advanced smart convergence
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    • v.8 no.3
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    • pp.27-38
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    • 2019
  • I reviewed about dyeing fastness and functional properties of the non-mordant dyed cotton and iron mordant dyed cotton with astringent persimmon juice. In the UV-VIS spectrum of astringent persimmon juice, the maximum absorption wavelength of the juice was 272.0 nm, which was a characteristic peak of tannin. In dyeing fastness, light fastness of the non-mordant dyed cotton was a little low at grade 2~3. But by the iron mordant treatment, the light fastness was good at grade 4. Dry cleaning fastness to discoloration and contamination was excellent at grade 4~5 for both the non-mordant dyed cotton and iron-mordant dyed cotton. Their rub fastness were grade 3 and grade 2~3 respectively, in the dry condition, and grade 2~3 and grade 3 respectively, in the wet condition. In the perspiration fastness of the non-mordant dyed cotton, the discoloration from acidity and alkalinity was grade 3~4 and grade 3, respectively, and contamination from acidity and alkalinity was relatively good at grade 4. Meanwhile, in the perspiration fastness of the non-mordant dyed cotton, discoloration from acidity and alkalinity was fair, both recording grade 3~4, and contamination from acidity and alkalinity was very good, both recording grade 4~5. In the ultraviolet protection effect of the non-mordant dyed cotton and iron-mordant dyed cotton, the ultraviolet protection factor was both 50+, both of which showed an excellent ultraviolet blocking effect. And the protection rate of UV-A appeared 98.4% and 99.1%, respectively, and the protection rate of UV-B showed 98.7% and 99.2, respectively. In addition, both exhibited an excellent deodorization rate over 99.9% or more, irrespective of the passage of time. Also, both showed an excellent antimicrobial activity over 99.9% or more against Staphylococcus aureus and Klebsiella pneumoniae.

Study on Quality Criteria for Transparent Soundproof Panels(2) - Sound Insulation Characteristics and Evaluation of Weatherability (투명방음판의 품질기준 설정에 관한 연구(2) - 음향 특성 및 내후성 평가)

  • Chang, Taesun;Kim, Chulhwan;Hwang, Cheolho
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.22 no.11
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    • pp.1099-1105
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    • 2012
  • Transparent noise barrier panels have the advantages of transmitting light to nearby residents and allowing drivers to orientate themselves by providing views of the surrounding area. To develop guidelines on quality criteria for transparent soundproof panels, their sound transmission loss and weatherability were experimentally investigated. In Korea, the sound transmission loss of noise barrier panels should be more than 25 dB at 500 Hz and 30 dB at 1000 Hz. The transmission loss tests of transparent panels were performed in accordance with KS F 2808. Typically, plastic materials suffer from color changes when exposed to ultraviolet(UV) light over extended periods. Therefore, weathering of plastics is one of the most important properties for outdoor applications. Protection against UV radiation and weathering is usually accomplished by using UV stabilizer additives or coatings. Transparent materials for soundproof panels were examined through accelerated weathering tests and their weathering resistances were evaluated from changes in yellowness index.

Photoprotective Effects of Soybean Extract against UV-Induced Damage in Human Fibroblast and Hairless Mouse Model

  • Cho, Young-Chang;Han, Jae-Bok;Park, Sang-Ik
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.1
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    • pp.20-29
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    • 2019
  • Soy isoflavones have been reported to possess many physiological activities such as antioxidant activity and inhibition of cancer cell proliferation. This study investigated the photoprotective effects of soybean extract in human fibroblast cell line and hairless mice model. Human fibroblast was treated with soybean extract before and after ultraviolet B (UVB; 290-302 nm) irradiation. In the soybean extract treated group, the cells showed better resistance to ultraviolet (UV) than control group. The amount of type I collagen recovered from the soybean treated group was higher than the vehicle group exposed to UV-induced damage. Moreover, increased expression of metalloproteinases-1 as a result of UV irradiation was suppressed by the soybean extract. Female mice were orally administered soybean extract and irradiated with UVB light for 8 weeks. The effects of the soybean extract on the skin appearance, collagen deposition and epidermal thickness in the UV-damaged mouse skin were analyzed using histopathological methods. In soybean extract treated group, the skin had a better morphology than that of the control group. Furthermore, the amount of type I collagen was increased and overexpression of MMP-1 was reduced in the soybean extract group compared to vehicle group. Additionally, up-regulation of pro-inflammatory cytokines induced by UV irradiation was suppressed by dietary soybean extract treatment. It appears that soybean extract had a photoprotective effect, including anti-aging and anti-inflammatory effect, from UV-induced damage in not only human fibroblast, but also hairless mice. We confirmed that these effects were possibly due to promotion of collagen synthesis and inhibition of MMP-1 expression.

UV Perspective for Dynamically Old and Young Clusters of Galaxies: Fornax and Virgo

  • Lee, Young-Dae;Rey, Soo-Chang;Pak, Min-A;Kim, Suk;Sung, Eon-Chang;Yi, Won-Hyeong;Chung, Ji-Won
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.2
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    • pp.76.1-76.1
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    • 2011
  • We present ultraviolet (UV) photometric properties of galaxies in two clusters, the Fornax and Virgo, with different dynamical conditions. We construct UV color-magnitude relations (CMRs) of galaxies in the Fornax and Virgo clusters using GALEX UV data matching with optical B band data. Elliptical and lenticular galaxies locate on red sequence in UV CMRs and show UV upturn phenomenon in both clusters. While dwarf lenticular galaxies (dS0s) in the Fornax also follow the extension of red sequence of giant early type galaxies, they are redder than dS0s in the Virgo at a given magnitude. We also investigated the effect of neighbor galaxies and cluster environment to the UV properties. In the space of projected clustercentric radius and projected nearest neighbor galaxy distance, we found that red (NUV-B>3) galaxy fraction of the Fornax depends entirely on clustercentric radius. However, in the case of Virgo, galaxy colors are also affected by interactions between galaxies outside the cluster virial radius. We suggest that UV properties of early-type galaxies in the Fornax cluster is likely consistent with its dynamically evolved system compared to the Virgo cluster.

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