• Title/Summary/Keyword: Ultrastructural

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Ultrastructural Localization of Mercury in Spleen of the Mouse (마우스 비장내 수은의 미세구조적 위치)

  • Cho, Hyun-Wook;Kim, Myung-Hoon;Yee, Sung-Tae
    • Applied Microscopy
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    • v.28 no.4
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    • pp.551-561
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    • 1998

  • The autometallographic method was used to demonstrate the localization of mercury deposits in spleen of mouse. The mercury deposits were identified with the light and electron mocroscope. Mice were treated with methylmercuric chloride in the drinking water (demineralized water) for 40 days. Control and mercury treated groups showed no significant differences in mean body weight and spleen weight per one mouse. Mercury grains were appeared in the germinal center of white pulp consist of a preponderancing lymphocytes, not in red pulp and capsule. At the ultrastructural level, mercury deposits were restricted to lysosomes of macrophage and lymphocyte. Specially, volume in lysosomes of the macrophage was increased. These results suggest that mercury localization in lysosomes is associated with the change of immune activity.

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Ultrastructural Changes in Midgut of CPV infected Tropical Tasar Silkworm, Antheraea mylitta (D) (Lepidoptera : Saturniidae)

  • Barsagade, Deepak Deewaji;Kadwey, Mangala Nimbaji
    • International Journal of Industrial Entomology and Biomaterials
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    • v.21 no.1
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    • pp.117-125
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    • 2010

  • The tropical tasar silkworms, Antheraea mylitta (D) produce famous silk 'Kosa' in central part of India. Due to outdoor rearing it became susceptible to viral infection including cytoplasmic polyhedrosis virus (CPV). The common mode of entry of cytoplasmic polyhedrosis virus is per os and cause gresserie disease to the larvae. Histopathological studies elucidated the insect CPV virus produces infective polyhedral inclusion bodies (PIBs) in the midgut cell cytoplasm of virus infected fifth instar larvae. The PIBs multiply enormously in the cytoplasm without invading the nucleus. Ultrastructural studies confirmed the pathological effects of CPV on in midgut cell cytoplasm. The multiplication of polyhedral inclusion bodies took place into the vacuoles and form virogenic stromata in the cytoplasm of cells. However, the encapsulations of polyhedral inclusion bodies into the polyhedrin protein occurred and polyhedra were released into the lumen. At the late stage of infection, cells showed the regressed cytoplasmic organelles with large vacuoles and elongated mitochondria. Hence, the horizontal transmission of CPV causing the midgut cells disintegration in the tasar silkworm, Antheraea mylitta (D) confirmed during infection.

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