• Journal of Chest Surgery
• /
• 제27권1호
• /
• pp.76-80
• /
• 1994

A solitary fibrous tumor in the pleura has been reported occasionally, but extremely rare in themediastinum. The histogenesis of this tumor has been still in controverse, but recent studies paid attention to it a mesenchymal origin by the immunohistochemical and ultrastructural bases. A few cases, reported in the literature, suggest that the mediastinal solitary fibrous tumor occurs more commonly in adult and shows slightly higher incidence in women. Its aggressive behavior such as recurrence rate and distant metastasis, is more prominant than reported in solitary fibrous tumor of the pleura. No single histologic feature allows an assumption definite prognosis. Surgical resection of this tumor is usually curative although the recurrence or distant metastasis are reported in about half of the patients. This report is a case of solitary fibrous tumor in the mediastinum in a 16-year-old female patient.She underwent surgical resection and her postoperative result was satisfactory.

• 대한기관식도과학회지
• /
• 제8권1호
• /
• pp.87-91
• /
• 2002

Solitary fibous tumor of pleura is a rare and slow vowing neoplasm. The histogenesis of this tumor is thought as mesenchymal origin by immunohistochemical and ultrastructural bases. This case report is of a 69-year-old female who presented severe dyspnea and orthopnea with long-term history. Preoperative chest X-ray an chest CT revealed huge and hemithorax-occupying heterogenous mass and cardiomegaly. She underwent surgical resection and a 22 X 18 X 10 cm sized visceral pleural based mass was evacuated. Histologic findings of tumor showed patternless proliferation in a fibrocollagenous and well vasculized stroma. Immnohistochemical staining revealed strong positive in CD34, that are compatible with solitary fibrous tumor of pleura. The patient was discharged without any complications and no evidence of recurrence was found after 2 years.

• 한국동물학회지
• /
• 제30권3호
• /
• pp.239-247
• /
• 1987

생쥐 신장의 근위세뇨관과 원위세뇨관 세포의 미세구조에 미치는 염화 메틸수은의 독성에 대한 홍삼유출물의 첨독성적 영향을 전자현징경적으로 연구하였다. 염화 메틸수은 처리군의 근위세뇨관 세포에서는 대조군에서 보다 철세 융모가 다소 축소되고 불규칙한 배열을 하였다. Brush border의 인접 부위에서는 작은 경계들이 나타나고 세포질 중앙 부위에서는 치밀체를 함유한 큰 육포들이 관색되었다. Mitochondria는 상당히 팽대되고 기양막은 부분적으로 비후되었으며 다수의 ITsosome이 나타났다. 염화 메틸수은 처리군의 원위세뇨관 세롱에서는 불규칙한 세포 표면, 양사된 세포, 그리고 다수의 ribosome과 소수의 지방사이 나타났으며 mitochondria 는 팽대되고 기고막은 부분적으로 비후되었다. 염화 메틸수은-홍삼추출물 병행 처리군의 근위세뇨관 세포에서는 염화 메틸수은 처리군에서 보다 mitochondria의 팽대 정도가 감소되었으며 육포의 크기와 수도 상당히 감소되었다. 염화 메틸수은-홍모적출물 병행 처리군의 원위세뇨관 세롱에서는 세포 표면이 어느정도 규칙적이고 mitochondria의 정해와 기저막의 비후 정도가 감소되어 정상 세포와 거의 유사하였다.

• 한국동물학회지
• /
• 제30권3호
• /
• pp.292-300
• /
• 1987

The ultrastructure of the tentacular pigment cells and the origin of the pigment granules in the Chinese mystery snail, Cipangopaludina chinensis malleata Reeve, are studied with electron microscope. Tentacular pigment cells of the Enail are the melanophores which contain electron dense melanosomes(melanin pigment granules) . Melanophores are distributed among the connective tissues but otter kind of dermal chromatephores are not observed. The epidermal melanin units are observed in the epithelial tissues of the tentacles. Among the several kinds of epithelial cells, only the epithelial supporting cells contain these pigment granules. Synthesis of the pigment materials is from the rough ER and pigment granules are finally being packaged and released by the Golgi complexes but limiting membrane of these granules are presumed to be originated from the smooth ER.

• Clinical and Experimental Reproductive Medicine
• /
• 제17권2호
• /
• pp.185-196
• /
• 1990

These experiments were performed to know ultrastructural changes of the cumulus expansion in virot. SEM:In expanded oocyte-cumulus complex, the cell surface are characterized by the presence of many evaginations:they are relatively short and round shape. The mucous extracellular material were deposited between cumulus cells. TEM:In compact cumulus cells, golgi apparatus and rough endoplasmic reticulum developed. In expanded cumulus cells, rough endoplasmic reticulum decreased and the smooth endoplasmic reticulum increased. Also, there were numbers of mitochondria. Extracellular mucous material which is presumed to be hyaluronic acid appears when cumulus cell were expanded. In expanded cumulus cell, numbers of smooth endoplasmic reticulum help cumulus cell to develop in steroidogenic cell.

• 한국동물학회지
• /
• 제36권4호
• /
• pp.496-504
• /
• 1993

한국산 플라나리아 Phagocata vivida의 인두에서 다음과 같은 점액과립들을 관찰하였는데 그 결과는 다음과 같다. 인두외강의 인두측 상피조직과 내측부의 유조직에서 조직화학적 및 미세구조적 특징에 따라 5종류의 점액과립(G1형, G2형, G3형, G4형 및 G5형)이 관찰되었다. methylene blue-baslc fuchsin 이중염색을 시행한 결과 G1형, G3형 및 G4형 과립은 methylene blue에만 양성반응을 보인데 비해 G2형 및 G5형 과립은 두 염색액에 모두 양성반응을 보였다. G1형과 G3형 과립은 그 크기가 0.5mm정도이고. 전자밀도가 매우 높게 나타났으며 특히 G1형 과립에서는 흰 반점과 줄무의가 관찰되었다 G2형 과립은 그 크기가 0.750m정도이고, 전자밀도는 G3형 과립에 비해 약간 낮았다. G4형 과립은 그 크기가 0.3mm정도로 매우 작고, 전자밀도는 낮아서 밝게 보였다. G5형 과립은 그 크기가 0.4$\times$0.3mm정도이고. 전자밀도가 낮게 나타났으며 배상세포 형태를 하고 있었다.

• 대한치과의사협회지
• /
• 제18권1호통권130호
• /
• pp.55-58
• /
• 1980

치주질환 발생시의 대식세포의 형태적 변화 및 기능적인 상관관계를 연구하고저 성견 5마리를 사용 실험하였다. 실험동물을 전신마취한 후 상하악 우측 제 1 및 제 2 소구치의 치경부에 1.3mm굵기의 wire를 4주간 결찰하여 인위적으로 치주염을 유발시켜 실험적으로 하였고, 동일동물의 좌측 제 1 및 제 2 소구치를 대조군으로 하였다. 실험군 및 대조군의 치아협측치은을 0.5 0.5 1mm의 형태로 절제하여 전자현미경적 관찰을 위한 통법의 조직처리를 한 다음 관찰하였다. 그 관찰 결과를 보면 다음과 같았다. 1. 실첨군의 치은의 고유층에 탐식성 돌기를 가진 많은 대식세포들이 출현하였다. 2. 변성된 교원 섬유군 인접부위에 phagocytic vesicle을 가진 대식세포들의 세포질내에 지방과립 및 잔존물을 볼 수 있었다. 한편 RER 및 Golgi complex는 매우 적었다. 3. 세포막은 그 기능상으로 많은 ruffles or folds를 나타냈으며 이 물질을 포식하는 양식을 뛰기도 하였다. pinocytotic activity를 띄는 대식세포들도 보였다. 4. 신생섬유아세포 주위에 밀접한 관계를 띄고 대식세포가 나타났으며 상호 유사하다. 대식세포에는 많은 residual body가 나타났고 RER은 매우 적었다.

• Applied Microscopy
• /
• 제46권4호
• /
• pp.171-175
• /
• 2016

A distinctive neuronal network in the brain is believed to make us unique individuals. Electron microscopy is a valuable tool for examining ultrastructural characteristics of neurons, synapses, and subcellular organelles. A recent technological breakthrough in volume electron microscopy allows large-scale circuit reconstruction of the nervous system with unprecedented detail. Serial-section electron microscopy-previously the domain of specialists-became automated with the advent of innovative systems such as the focused ion beam and serial block-face scanning electron microscopes and the automated tape-collecting ultramicrotome. Further advances in microscopic design and instrumentation are also available, which allow the reconstruction of unprecedentedly large volumes of brain tissue at high speed. The recent introduction of correlative light and electron microscopy will help to identify specific neural circuits associated with behavioral characteristics and revolutionize our understanding of how the brain works.

• Applied Microscopy
• /
• 제28권4호
• /
• pp.577-590
• /
• 1998

Puromycin aminonucleoside (PAN) nephropathy was induced in a group of Sprague-Dawley rat by a single dose of intraperitoneal Injection to study an ultrastructural change of glomerulus. The experimental rats developed proteinuria three days after PAN injection. Electron microscopic studies of glomeruli showed the loss of epithelial foot processes, formation of cytoplasmic vacuoles, microvillous formation and increased numbers of lysosomes in the cytoplasm of podocytes. It is strongly suggested that proteinuria in PAN nephrosis may be primarily due to a glomerular epithelial lesion, leading to focal disarray of anionic sites or focal defects in the epithelial covering of the basement membrane. The loss of anionic sites in the basement membrane nay be caused by the foot process fusion and the epithelial detachment from the basement membrane.

• Applied Microscopy
• /
• 제28권2호
• /
• pp.181-191
• /
• 1998

The spermatogenesis of pale chub (Zacco platypus) was investigated morphologically. The testis of pale chub contained numerous testicular sacs. These testicular sacs were bounded on neighboring sacs by single layer of squamous cells. Also, differentiated sperms were filled in the sacs. In the stage of spermatogonium, the germ cells had a large nucleus and a distinct nucleolus, and mitochondrial development was prominent. In the primary and secondary spermatogonia, these cells had a round electron-dense nucleus, reduced cytoplasm, and mitochondria were congregated in the side of cytoplasm. The highly condensed chromatin of sperms was electron-dense, the acrosome was not found in the head of the sperm and a motile flagellum consisted of an axoneme with a typical 9+2 pattern of microtubules.