• Title/Summary/Keyword: UVS

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UVSC of Aspergillus nidulans is a Functional Homolog of RAD51 in Yeast

  • Yoon, Jin-Ho;Seong, Kye-Yong;Chae, Suhn-Kee;Kang, Hyen-Sam
    • BMB Reports
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    • v.34 no.5
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    • pp.428-433
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    • 2001
  • A defect in uvsC of Aspergillus nidulans caused high methyl methansulfonate (MMS)-sensitivity, hyporecombination, and a lack of UV induced mutation. The uvsC gene of Aspergillus nidulans shares a sequence similarity with the RAD51 gene of Saccharomyces cerevisiae. In this study, in vitro and in vivo tests were conducted in order to determine whether or not the UVSC protein had functional similarities to RAD51, the recombination enzyme in yeast. The purified recombinant UVSC protein, following expression in Escherichia coli, showed binding activity to single-stranded DNA (ssDNA), when both ATP and magnesium are present. In addition, ATPase activity was also demonstrated and its activity was stimulated in the presence of ssDNA. The UVSC protein that was expressed under the ADH promoter in S. cerevisiae suppressed in part the sensitivity to MMS of the rad51 null mutant. Similarly, when the uvsC cDNA was expressed from the nmt promoter, the MMS sensitivity of the rhp51 null mutant of Schizosaccharomyces pombe was partially complemented. These results indicate that the A. nidulans UVSC protein is a functional homologue of the RAD51 protein.

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Development of UVS for the seamless u-Transportation servic (Seamless u-Transportation 서비스를 위한 UVS 개발)

  • Kim, Do-Yoon;Sung, Sang-Hak;Park, Jong-Ho;Lee, Jae-Hoon;Park, Ju-Young
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.1629_1630
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    • 2009
  • 유비쿼터스 교통 환경에서 GNSS 전파 수신 신호 상태와 상관없이 차량의 위치 정보 및 차량의 돌발 상황에 대한 서비스를 제공하기 위한 관성 항법 장치의 개발에 대해 소개한다. 제안된 시스템은 위치 파악에 많은 오차를 유발시키는 고도 부분을 분리하였고, GNSS 신호 유무에 상관없이 동작할 수 있는 시스템을 제안하였다. IMU에서는 100Hz의 속도로 위치를 파악하며, 움직이는 차량의 위치를 120초 이내에서는 GNSS 전파 수신하는 환경과 유사한 위치 오차 내에서 동작이 가능함을 실험을 통해 보였다.

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Mobile Robot Localization using Ubiquitous Vision System (시각기반 센서 네트워크를 이용한 이동로봇의 위치 추정)

  • Dao, Nguyen Xuan;Kim, Chi-Ho;You, Bum-Jae
    • Proceedings of the KIEE Conference
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    • 2005.07d
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    • pp.2780-2782
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    • 2005
  • In this paper, we present a mobile robot localization solution by using a Ubiquitous Vision System (UVS). The collective information gathered by multiple cameras that are strategically placed has many advantages. For example, aggregation of information from multiple viewpoints reduces the uncertainty about the robots' positions. We construct UVS as a multi-agent system by regarding each vision sensor as one vision agent (VA). Each VA performs target segmentation by color and motion information as well as visual tracking for multiple objects. Our modified identified contractnet (ICN) protocol is used for communication between VAs to coordinate multitask. This protocol raises scalability and modularity of thesystem because of independent number of VAs and needless calibration. Furthermore, the handover between VAs by using ICN is seamless. Experimental results show the robustness of the solution with respect to a widespread area. The performance in indoor environments shows the feasibility of the proposed solution in real-time.

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Bird's-Eye View Service under Ubiquitous Transportation Sensor Network Environments (Ubiquitous Transportation Sensor Network에서 Bird's-Eye View 서비스)

  • Kim, Joohwan;Nam, Doohee;Baek, Sungjoon
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.13 no.2
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    • pp.225-231
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    • 2013
  • A bird's-eye view is an elevated view of an object from above, with a perspective as though the observer were a bird, often used in the making of blueprints, floor plans and maps. It can be used under severe weather conditions when visibility is poor. Under low visibility environments, drivers can communicate each other using V2V communication to get each vehicle's status to prevent collision and other accidents. Ubiquitous transportation sensor networks(u-TSN) and its application are emerging rapidly as an exciting new paradigm to provide reliable and comfortable transportatione services. The ever-growing u-TSN and its application will provide an intelligent and ubiquitous communication and network technology for traffic safety area.

Identification and Cloning of jipA Encoding a Polypeptide That Interacts with a Homolog of Yeast Rad6, UVSJ in Aspergillus nidulans

  • Cho, Jae-Han;Yun, Seok-Soong;Jang, Young-Kug;Cha, Mee-Jeong;Kwon, Nak-Jung;Chae, Suhn-Kee
    • Journal of Microbiology
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    • v.41 no.1
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    • pp.46-51
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    • 2003
  • RAD6 in yeast mediates postreplication DNA repair and is responsible for DNA-damage induced mutations. RAD6 encodes ubiquitin-conjugating enzyme that is well conserved among eukaryotic organisms. However, the molecular targets and consequences of their ubiquitination by Rad6 have remained elusive. In Aspergillus nidulans, a RAD6 homolog has been isolated and shown to be an allele of uvs). We screened a CDNA library to isolate UVSJ-interacting proteins by the yeast two-hybrid system. JIPA was identified as an interactor of UVSJ. Their interaction was confirmed in vitro by a GST-pull down assay. JIPA was also able to interact with mutant UVSJ proteins, UVSJl and the active site cysteine mutant UVSJ-C88A. The N- and the C-terminal regions of UVSJ required for the interaction with UVSH, a RAD18 homolog of yeast which physically interacts with Rad6, were not necessary for the JIPA and UVSJ interactions. About 1.4 kb jipA transcript was detected in Northern analysis and its amount was not significantly increased in response to DNA-damaging agents. A genomic DNA clone of the jipA gene was isolated from a chromosome I specific genomic library by PCR-sib selection. Sequence determination of genomic and cDNA of jipA revealed an ORF of 893 bp interrupted by 2 introns, encoding a putative polypeptide of 262 amino acids. JIPA has 33% amino acid sequence identity to TIP41 of Saccharomyces cerevisiae which negatively regulates the TOR signaling pathway.

Ultraviolet Inactivation of Escherichia coli in Stainless Steel Cups (스테인리스스틸 컵 내 Escherichia coli의 자외선 살균)

  • Mok, Chulkyoon;Lee, Nam-Hoon
    • Food Engineering Progress
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    • v.13 no.2
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    • pp.122-129
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    • 2009
  • Ultraviolet (UV) is widely used as a sterilizing agent in restaurants and catering facilities in Korea. Efficacy of UV sterilizers (UVS) was investigated against E. coli on the inner bottom of stainless steel cups. UV intensity on the bottom of cups varied widely with the locations of cups in UVS, showing higher values at center while lower values at outskirts. The deviations in UV intensity were remarkable on top shelf, but alleviated as proceeded to middle and bottom shelves. Inactivation of E. coli was proportional to the UV intensity and treatment time, consequently to UV dose, and showed a pseudo-first-order kinetics with tailing. Initial inactivation rate constants ($K_{1}$) deviated with the locations of the cups, while final inactivation rate constants ($K_{2}$) showed comparable values. An equation for the calculation of the proposed UV treatment time was suggested.

Observation of a 2016 Ganymede stellar occultation event with the SOAO 0.6m telescope

  • Hinse, Tobias Cornelius;D'Aversa, Emiliano;Oliva, Fabrizio;Sindoni, Giuseppe
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.2
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    • pp.83.3-84
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    • 2017
  • On 2016 April $13^{th}$ the Jovian satellite Ganymede occulted a $7^{th}$ magnitude star. The predicted occultation track (occultation shadow) crossed the Northern Pacific Ocean, Japan, and South Korea. Hence, it was a very favorable event due to the star brightness in order to be accessible for small-aperture telescopes as well. While no other similar event is expected for the next 10 years, only two occultation events are reported in the literature in the past, from Earth in 1972 and from Voyager, in large disagreement in respect to the atmospheric detection. However, evidence of an exosphere around Ganymede was inferred through H Lyman alpha emission detected by Galileo UVS, through HST/GHRS detection of far-UV atomic O airglow emissions, signature of dissociated molecular oxygen. We organized a short-notice international coordinated occultation monitoring network with the aim to search for a signature of Ganymede's exosphere in the occultation light-curve by using facilities on Mauna Kea (NASA-IRTF) and Sobaeksan Optical Astronomy Observatory (SOAO) in South Korea.

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A Novel Method for Estimating Representative Section Travel Times Using Individual Vehicle Trajectory Data (개별차량 주행정보를 이용한 차로별 구간대표통행시간 산출기법)

  • Rim, Hee-Sub;Oh, Cheol;Kang, Kyeong-Pyo
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.8 no.6
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    • pp.23-35
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    • 2009
  • This study proposes a methodology for estimating representative section travel times using individual vehicle travel information under the ubiquitous transportation environment (UTE). A novel approach is to substantialize a concept of dynamic node-links in processing trajectory data. Also, grouping vehicles was conducted to obtain more reliable travel times representing characteristics of individual vehicle travels. Since the UTE allows us to obtain higher accuracy of vehicle positions, travel times for each lane can be estimated based on the proposed methodology. Evaluation results show that less than 10% of mean absolute percentage error was achievable with 20% of probe vehicle rate. It is expected that outcome of this study is useful for providing more accurate and reliable traffic information services.

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