• Title/Summary/Keyword: UV-A

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Action Spectra of Apoptosis Induction and Reproductive Cell Death in L5178Y cells in UV-B Region

  • Mizuho Aoki;Yoshiya Furusawa;Higashi, Sho-ichi;Masakatsu Watanabe
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.454-456
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    • 2002
  • It is important to determine the action spectrum of UV-B radiation contained in the sunlight to estimate the risk of skin cancer. We have investigated action spectra for induction of apoptosis and reproductive cell death in L5178Y cells using the Okazaki Large Spectrograph at NIBB. L5178Y cells were exposed to light at different wavelengths in UV-B or UV-A region. Frequencies of apoptosis induction and reproductive cell death were determined by counting cells with chromatin condensation, and by the colony formation assay, respectively. The measured sensitivity spectra for the two end-points were in very good agreement. Sensitivity decreased steeply with increase of wavelength in UV-B region and remains nearly constant in UV-A region. The action spectra were also slightly steeper than that for the minimum erythematic dose (MED), but very similar to the light absorption spectrum of DNA in UV-B region. On the other hand, the spectra for both endpoints were similar to MED spectrum but not DNA spectrum in the UV-A region. Also different time-course and morphological difference of apoptosis were found between UV-B (long time, fragmentation) and UV-A (short time, shrinkage) region. These results suggest that DNA damage induced by UV-B light triggers apoptosis and reproductive cell death, but other damaged targets (membrane, protein and so on) trigger these effects in UV-A region.

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Single-step UV nanoimprint lithography on a 4" Si wafer (4" Si 웨이퍼에 대한 single-step UV 나노임프린트 리소그래피)

  • 정준호;손현기;심영석;신영재;이응숙;최성욱;김재호
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2003.06a
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    • pp.199-202
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    • 2003
  • Ultraviolet-nanoimprint lithography (UV-NIL) is a promising method for cost-effectively defining nanoscale structures at room temperature and low pressure. Since the resolution of nanostructures depends strongly upon that of nanostamps, the nanostamp fabrication technology is a key technology to UV-NIL. In this paper, a 5$\times$5$\times$0.09 in. quartz stamp whose critical dimension is 377 nm was fabricated using the etch process in which a Cr film was employed as a hard mask for transferring nanostructures onto the quartz plate. To effectively apply tile fabricated 5-in. stamp to UV-NIL on a 4-in. Si wafer, we have proposed a new UV-NIL process using a multi-dispensing method as a way to supply resist on a wafer Experiments have shown that the multi-dispensing method can enable UV-NIL rising a large-area stamp.

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Physio-Morphological Changes in a Riboflavin Producer Eremothecium ashbyii DT1 and UV Mutants in Submerged Fermentation

  • Pujari, Venugopal;Chandra, T.S.
    • Journal of Microbiology and Biotechnology
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    • v.11 no.4
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    • pp.552-557
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    • 2001
  • By UV-irradiation of Eremothecium ashbyii DTl, a higWy flavinogenic mutant (UV-18-57) and a nonflavinogenic mutant (UV -85) were obtained. The physio-morphological characteristics of these three strains were studied on glucose medium in submerged fermentation. Glucose utilization and mycelial growth occurred in 0 - 2 days of fermentation. By the third day, the biomass had declined. Extracellular riboflavin excretion was distinct from the second day, reaching a maximum rate by the fourth day. The hyphae of the highly flavinogenic mutant UV-18-57 were broader than DTl, while the nonflavinogenic UV-85 hyphae were very thin. Riboflavin accumulation was high in UV-18-57 (extracellular riboflavin,$825\mu\textrm{g}/ml$ , and intracellular, $490\mu\textrm{g}/ml$) and caused the mycelia to swell into bulbous forms. Riboflavin accumulation was less in DTl ($108\mu\textrm{g}/ml$ extracellular and $24\mu\textrm{g}/ml$ intracellular) and correspondingly its hyphae were thinner than those of UV-18-57 and swollen bulbous mycelia were not prominent. UV-85 was nonflavinogenic and, accordingly, its mOlphological characteristics included long thin filaments with no intracellular riboflavin accumulation. A large number of greenish fluorescence spores were seen in UV-18-57, whereas DTI had less spores and UV-85 was nonsporulating. Sporulation is correlated with riboflavin production. UV-18-57 had better mycelial integrity and lysis started only by the seventh day, whereas DTI and UV -85 started to lyze earlier by 4 -5 days. By the late stage of fermentation (eighth day), DTl had a few long, thin filaments indicating some secondary growth, whereas UV -85 showed a compact pellet form of mycelia. Most mycelia of UV-18-57 still appeared intact.

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Elicitation of Chilling Tolerance of Pepper Seedlings Using UV-A LED (UV-A LED을 이용한 고추 묘의 저온 내성 유도)

  • Park, Song-Yi
    • Korean Journal of Environmental Agriculture
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    • v.39 no.3
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    • pp.273-279
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    • 2020
  • BACKGROUND: After transplanting, the recent abnormal low temperature caused physiological disorders of pepper seedlings. This study was conducted to evaluate the effects of UV-A LED, a physical elicitor, on the chilling tolerance of pepper seedlings. METHODS AND RESULTS: Seedlings were continuously irradiated with 370 and 385 nm UV-A LEDs with 30 W·m-2 for 6 d. After that, seedlings were exposed to 4℃ for 6 h and then recovered under the normal growing condition for 2 d. There were no significant differences in growth characteristics of UV-A treatments compared to the control. Fv/Fm values of two UV-A treatments were below 0.8. Electrolyte leakage in the control was increased by chilling stress, while 385 nm UV-A had the significantly lowest value. Total phenolic content and antioxidant capacity of two UV-A treatments significantly increased due to UV-A radiation. However, total phenolic content and antioxidant capacity of the control increased due to chilling stress and tended to decrease again during the recovery time. CONCLUSION: We confirmed that UV-A light was effective to induce the chilling tolerance of pepper seedling, and the supplemental radiation of 385 nm UV-A LED before transplanting could be used as a cultivation technique to produce high quality pepper seedlings.

Inactivation of Mycobacterium using Ultrasonic and Ultraviolet Sequential Processes (초음파와 자외선 연계공정을 이용한 Mycobacterium 불활성화)

  • Kim, Wangi;Jung, Yeonjung;Yoon, Yeojoon;Lim, Gwanhun;Kim, Jongbae;Kang, Joon-Wun
    • Journal of Korean Society on Water Environment
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    • v.28 no.1
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    • pp.57-62
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    • 2012
  • In this study, the inactivation efficiency of Mycobacterium marinum was evaluated in buffered water (pH 7) using a low pressure ultraviolet (LP-UV) lamp, ultrasonic (US), and UV/US sequential processes. In the UV alone process, 3 log inactivation of the M. marinum was achieved with a UV dose of $120mJ/cm^2$. However, a tailing phase was later observed because M. marinum has a high tendency for cell aggregation. Even though the M. marinum was not inactivated in the US alone process, the hydrophobicity decreased and turbidity increased due to the crumbling of the cell aggregation. Among the candidate processes which were UV alone, US-UV sequential process and UV-US-UV sequential process, the US-UV sequential process showed the highest synergistic effects for M. marinum inactivation. Consequently, US is a very useful process as a UV irradiation pre-treatment to inactivate M. marinum in water.

탐방 - (주)IUV

  • Park, Seong-Gwon
    • 프린팅코리아
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    • v.9 no.5
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    • pp.102-105
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    • 2010
  • UV인쇄가 비약적인 발전을 거듭, 특수인쇄의 범주를 벗어나 일반화되고 있다. UV에 대한 메리트가 일반 소비자뿐 아니라 인쇄업자에게 크게 어필도기 때문이다. 이에 (주)IUV(대표이사 이승규)는 오프라인 인쇄기에 UV인쇄가 가능토록 설비를 전문으로 하고 있다. (주)UV은 기계의 용도와 사양에 맞춰 UV건조장치를 연구 개발 설치 A/S UV교육 등 UV환경을 만들어 내는 독보적인 UV전문업체다. 경기도 부천시 오정구 삼정동에 본사를 두고 있는 (주)UV는 양주시 덕계동에 제작현장, 안양시 동안구 호계동에 연구실을 두고 현장에 실용적으로 대응하는 시스템을 구축하고 우리나라 인쇄업계의 UV화에 선도적인 역할을 수행해 오고 있다.

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Photocatalytic Oxidation of Han River Humic Substances and Change of Their Characteristics by $TiO_2/UV$ in a Rotating Photoreactor ($TiO_2/UV$ 회전반응기를 이용한 한강 휴믹물질의 광촉매산화 처리 및 특성 변화)

  • Shin, Jee-Won;Kim, Hyun-Chul;Han, Ihn-Sup
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.10
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    • pp.1129-1135
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    • 2005
  • In this study. the applicability of a rotating reactor for the oxidative removal of aqueous humic substances extracted from the Han River in Seoul, Korea was investigated. As air blowing for proper mixing of $TiO_2$ photocatalyst could inhibit UV-irradiation between a UV lamp and photocatalyst by air bubbles, a rotating reactor with some baffles was used for better UV-irradiation effect in this study. Han River humic substances are different from the other commercial humic substances(e.g., from Aldrich and International Humic Substance Society). Their characteristics were investigated with structural and spectroscopic analyses using FT-IR(Fourier transform-infrared), and $^{13}C$-NMR (nuclear magnetic resonance). The humic substances were extracted by XAD-7HP and treated with $TiO_2$-coated hollow beads under UV-A and UV-C irradiation in order to solve problems of separation and recovery of photocatalyst after reaction. At approximately 5 mg/L of initial TOC concentration, pH 3 and $2.0\;g-TiO_2/L$ dose, photocatalytic oxidation of Han River humic substances showed the optimum removal efficiency. Also, UV-C and UV-A lamps showed similar TOC removal efficiency. However, under UV-C irradiation, Han River humic substances were degraded to smaller compounds and increased the proportion of low molecular weight fractions compared to UV-A.

A Study on the Effect on UV Exposure in Coastal Buildings (연안건축물의 자외선 노출에 따른 안전성 연구)

  • Kim, Taehwan;Uh, Jesun
    • Journal of the Society of Disaster Information
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    • v.17 no.2
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    • pp.195-205
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    • 2021
  • Purpose: The ultraviolet reflectance and transmittance of coastal building materials are one of the important factors of ultraviolet radiation in and out of coastal building. In this research, the ultraviolet spectral reflectance of many kinds of building materials was measured. Also, the relationships with the lightness, roughness, and chromaticity, which are surface characteristics, were reviewed and suggested. Method: In this study, according to the CIE classification, the ultraviolet region was defined as short-wavelength region UV-C(10nm~280nm), medium-wavelength region UV-B (280-315 nm), and long-wavelength region UV-A (315-400nm), and the visible light region was defined as (400nm~780nm). Spectrophotometer was used to continuously measure the reflectance from the ultraviolet region to the visible light region. Results: From the measurement results, the ultraviolet reflectance on Wood was shown to be about Visible 55-68%, UV-A* 7-12%, and UV-B 4-5%. Wall tiles are about Visible18-40%, UV-A* 8-20%, and UV-B* 7-8%. That on concrete was shown to be about Visible 37%, UV-A* 28%, and UV-B*19%. Conclusion: The ultraviolet reflectance can be estimated by visible reflectance. Also, it is important to select a variety of materials according to the application when blocking UV.

UV transparent stamp fabrication for UV nanoimprint lithography (UV 나노임프린트 리소그래피용 UV 투과성 나노스탬프 제작)

  • Jeong, Jun-Ho;Sim, Young-Suk;Sohn, Hyon-Kee;Shin, Young-Jae;Lee, Eung-Suk;Hur, Ik-Boum;Kwon, Sung-Won
    • Proceedings of the KSME Conference
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    • 2003.04a
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    • pp.1069-1072
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    • 2003
  • Ultraviolet-nanoimprint lithography (UV-NIL) is a promising nanoimprint method for cost-effectively defining nanometer scale structures at room temperature and low pressure. Nanostamp fabrication technology is a key technology for UV-NIL because fabricating a high resolution nanostamp is the first step for defining high resolution nanostructures in a substrate. We used quartz as an UV transparent stamp material for the UVNIL. A $5{\times}5{\times}0.09$ inch stamp was fabricated using the quartz etch process in which Cr film was used as a hard mask for transferring nanostructures into the quartz. In this paper, we describe the quartz etching process and discuss the results including SEM images.

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Growth Responses at Different Growth Stage of Pinus densiflora Seedlings to Enhanced Uv-B Radiation (자외선-B 증가에 따른 소나무 유묘의 생장 단계별 생장 반응)

  • 김종진
    • Journal of Korea Foresty Energy
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    • v.19 no.1
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    • pp.1-6
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    • 2000
  • This study was carried out to investigate the growth responses of Pinus densiflora seedlings to enhanced UV-B environment for 16 weeks in the field condition. The seedlings were treated with one of three levels of UV-B dosages - ambient UV-B, ambient + 3.2, and ambient + 5.2 KJ m$^{-2}$day$^{-1}$ and the irradiation was performed at the stage before the germination, the fully expanded cotyledon, and the primary needles grown more than 0.8cm in length of the seedlings, respectively. Enhanced UV-B irradiation reduced the height and the root collar diameter growth, and dry mass production of the seedling, and T/R ratio was increased by the UV-B treatment. Difference in seedling growth was observed by difference in time of the UV-B treatment. Among the seedlings which were treated with ambient - 3.2 KJ m$^{-2}$day$^{-1}$, height and root collar diameter growth was relatively high in the seedling received the UV-B treatment at the stage before the germination. The lowest dry mass production was observed in the seedlings received the UV-B at stage of cotyledon both in two levels of enhanced UV-B treatment. Chlorophyll concentration was reduced by enhanced UV-B irradiation, and chlorophyll a/b ratio was increased by the UV-B treatment.

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