• 대한화학회지
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• 제34권6호
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• pp.616-621
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• 1990

UV-VIS 흡광광도법으로 Nd, Pr, Sm, Eu, Ho 및 Er을 정량할 때 스펙트럼 방해를 다원소 보정법으로 방법에 대하여 연구하였다. 개별희토류의 흡광도를 측정하고 또, Nd와 Pr의 혼합용액 및 Sm, Eu, Ho, Er의 혼합용액의 흡광도를 측정하여 몰흡광 계수를 구하고 이들로부터 상호 방해가 가장 적은 파장 즉, Nd(444.2nm), Pr(575.1nm), Sm(401.7nm), Eu(394.2nm), Ho(416.6nm), Er(379.7 nm)임을 알았다.

• 분석과학
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• 제34권5호
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• pp.212-224
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• 2021

The intent of the present work was to develop a simple, sensitive, accurate, precise, rapid and economical UV- spectrophotometric and reverse phase high pressure liquid chromatographic method for the simultaneous estimation of Spironolactone and Furosemide in bulk and combined tablet dosage forms. UV-Spectrophotometry was carried out by simultaneous equation method using 0.02 M potassium dihydrogen phosphate buffer pH 3.5: Acetonitrile (50:50) v/v as a solvent. The linearity range was 2-14 ㎍ mL^-1 for Spironolactone and Furosemide with a correlation coefficient > 0.99. The chromatographic separation was achieved on 250 mm × 4.6 mm, hypersil BDS C18 column with particle size 5 ㎛, by using an isocratic mixture of 0.02 M potassium dihydrogen phosphate buffer pH 3.5: Acetonitrile: tert butyl methyl ether (49:50:1) v/v/v as a solvent at a flow rate of 1 mL min^-1 and UV detection was carried out at 254 nm. The retention time were observed to be 3.666 and 6.661 minutes for Furosemide and Spironolactone respectively. The two developed methods were validated according to the ICH guidelines for accuracy, precision, linearity, LOD, LOQ and were found to be within the limits. It can be concluded that these two methods could be successfully used for the simultaneous estimation of Spironolactone and Furosemide in bulk and combined tablet dosage forms.

• 한국작물학회지
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• 제51권1호
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• pp.95-100
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• 2006

Sesamin and sesamolin, antioxidant lipidsoluble lignan compounds, are abundant in sesame (Sesamum indicum L.) seed oil and provide oxidative stability of oil related to sesame quality. The sesamin and sesamolin contents of 403 sesame land races of Korea were determined by HPLC analysis of methanol extract (HPLC value), and their total lignan content was compared with those by using UV-Vis spectrophotometric analysis (UV method) of methanol (UV-MeOH value) and hexane (UV-Hexane value) extracts. HPLC values of total lignan content were strongly associated with UV-Hexane (r=0.705**) and UV-MeOH (r=0.811**) values. The UV values from both the extracts were 3.8-4.7 times higher than those of HPLC values. Lignan content was overestimated by UV method because total compounds in the mixture solution were quantified by absorbing at the same ultraviolet wavelength as in HPLC method. UV method could more rapidly analyze small amount of sample with higher sensitivity of detection than HPLC method. Average contents of lignans in sesame germplasm evaluated in this study were $2.09{\pm}1.02mg/g$ of sesamin, and $1.65{\pm}0.61mg/g$ of sesamolin, respectively, showing significant variation for lignan components. The results showed that UV method for the determination of sesamin and sesamolin could be practically used as a faster and easier method than HPLC by using the regression equations developed in this study.

• 한국환경보건학회지
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• 제24권3호
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• pp.1-5
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• 1998

A spectrophotometric method was developed for the acidic solution stripped after an extraction of 0.5 to 2.5 ppm of Lead(II) from 50 mL of $Na_2S_2O_3$ solution into chloroform as the ion-pairs formed between their thiosulfate complexes and alkylamine, Aliquat336. Pb(II) in the stripped solution forms an complex with DDTC in pH 7.3 buffer solution, and was developed in yellow by copper replacement. The ydlow-colored solution have the maximum absorbance at 435 nm in the measurement of absorbance by UV-Visible spectrophotometer. The interference ions such as Fe(III), Hg (II), Al(III), Co, Cu, Ni, Zn, Ca, Sn, have great effects on the extraction, but they were overcomed by the usage of adequate masking agents before an extraction. At last, a good result was obtained in applying this method to synthetic water.

• Archives of Pharmacal Research
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• 제12권3호
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• pp.160-165
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• 1989

Binding of sulfaethidole to bovine serum albumin was studied by equilibrium dialysis, fluorescence probe technique, uv difference spectrophotometry and circular dichroism. Equilibrium dialysis method enabled us to estimate the total number of drug binding sites of albumin molecule. For sulfaethidole, albumin had 6 primary and 40 secondary binding sites. The primary and secondary binding constants were 0.9 * 10/sup 5/ M/sup -1/ and 0.2 * 10/sup 6/ M/sup -1/, respectivitely. 1-Anilino-8-naphthalenesulfonate (ANS) and 2-(4-hydroxylbenzeneazo)- benzoic acid (HBAB) were used as the fluorescence probe and the uv spectrophotometric probe, respectively. In fluorescence probe technique, results indicated that the number of higher affinity drug binding site of albumin was 1 and the number of lower affinity drug binding sites of albumin was 3, and the primary and secondary drug binding constants for bovine serum albumin were 2.15 * 10/sup 5/M/sup -1/ and 1.04 * 10/sup 5/ M/sup -1/, respectively. In uv difference spectrophotometry, binding sites were 3 and binding constant was 1.88 * 10/sup 5/M/sup -1/. The above spectrophotometry, binding sites were 3 and binding constant was 1.88 * 10/sup 5/M/sup -1/. The above results suggest that several different methods should be used in ompensation for insufficient information about drug binding to albumin molecule given by only one method.

• 약학회지
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• 제34권1호
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• pp.22-33
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• 1990

Aqueous solution of bis(2,4-diaminophenyl)phosphonate(APP) was very stable, especially below pH 2.0 and the red-color compound formed by the reaction of APP and $IO_3-$ was stable at room temperature. A simple and rapid spectrophotometric method for the determination of ascorbic acid, potassium antimonyl tartrate (PAT), and isonicotinic acid hydrazide (INAH) was established by the reaction of $IO_3-$ and these reducing drugs, and the absorbance measurements were made at 500 nm. In the reaction of $IO_3-$ and each of the reducing drugs, the conditions of pH were suitable below 2.5 for ascorbic acid, below 2.0 for PAT, and below 1.5 for INAH. Beer's law did hold in the range of $17.6{\sim}1549.9\;ug$ for ascorbic acid, $33.4{\sim}2871.8{\mu}g$ for PAT,and $6.9{\sim}548.6\;{\mu}g$ for INAH. Many common ingredients present in pharmaceutical dosage forms did not interfere. The average recoveries for ascorbic acid and INAH in pharmaceutical formulations were 99.8 $-100.3\;{\pm}\;0.2{\sim}0.4%$, $99.8\;{\pm}\;0.3%$, respectively.

• 대한화장품학회지
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• 제24권3호
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• pp.81-88
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• 1998

The aim of this study is to evaluate several plant extracts with a view to developing UV sunscreen agents. In this study, 150 plant extracts were screened to elucidate their UV spectra using spectrophotometric method. Several plant extracts such as Phellodendron amurense, Morus alba, Rhododendron mucronulatum, Brassica alba have strong absorbency at UVA region (350nm), the suntanning wavelength. And Sophora flavescens, Caesalpinia sapper, Morus alba, Phellodendron amurense, showed absorption plateau value at UVB region (308nm), the erythema action wavelength. These extracts have a good absorbency property as synthetic filter and could be served as substitutes for synthetic UV sunscreen agents.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2006년도 PAN PACIFIC CONFERENCE vol.1
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• pp.137-140
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• 2006

This paper describes a technique combining chemometrics with UV spectroscopy for the determination of the concentra tions of two tissue additives (i.e., wet strength and softening agents) in a cellulose fiber containing solution. In single as ent solutions, the concentration of the additive can be measured by UV spectroscopy at the wavelength where the species having absorption. For a binary (i.e., containing two additives) solution system, the spectral characterization is very complicated. However, if aided by a chemometrical calibration technique, each additive in the binary solution can be quantified simultaneously. The present method is very rapid and simple, it can easily perform a continuous measurement in the changes in the additives' concentration after fiber addition, and therefore this becomes a valuable tool for the adsorption kinetics study of chemical additives onto the cellulose fibers. The time-dependent adsorption behaviors of the wet-strength, softening agent, and their both on fiber were also presented.

• 분석과학
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• 제20권1호
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• pp.25-32
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• 2007

본 연구에서는 분석방법에 따른 고농도 질소함유 폐수의 총질소 측정결과를 비교평가하였다. 수질오염공정시험방법에 제시된 자외선 흡광광도법과 환원증류 킬달법을 주 시험법으로 하였으며, 추가적으로 TKN(total Kjeldahl nitrogen)법과 이온 크로마토그래피법(Ion chromatography)을 사용하였다. 자외선 흡광광도법은 실험방법이 비교적 간단하지만 고농도 폐수시료의 희석과 표준용액 제조에 있어서 큰 오차를 유발하였다. 환원증류 킬달법은 시료의 희석이 필요하지 않으며 가장 높은 측정값을 보였지만, 데발다 합금 및 NaOH 첨가량에 의해 최대 50 mg/L의 오차가 발생하였다. 동일한 시료를 반복하여 실험한 결과, 환원증류 킬달법($568.6{\pm}38.7mg/L$) > 자외선 흡광광도법($527.3{\pm}9.6mg/L$) > TKN법($494.7{\pm}21.4mg/L$) >이온 크로마토그래피법($417.9{\pm}7.3mg/L$)의 순으로 측정되었다. 그러므로 기타 분석법에 의한 과소평가 가능성을 고려하면, 환원증류 킬달법을 사용하는 것이 고농도 총질소 분석 측면에서 바람직할 것이다. 그러나 각 분석법과 시료의 특성에 따라서 실험 단계별로 최적분석조건을 확인하고, 더 나아가 표준작업지침서(Standard Operation Procedure: SOP)를 작성하여 분석 신뢰도를 향상시킬 필요가 있다.

• 한국조명전기설비학회지:조명전기설비
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• 제11권1호
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• pp.99-105
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• 1997

본 논문에서는 주상변압기 절연유의 가속열화반응을 수행하였다. 유중가스를 air bubbling 법으로 추출하여 가스크로마토크라프로 성분을 분석하였으며 가스농도는 각 가스의 추출율을 감안하여 보정하였다. 절연재료의 열화는 열분해반응과산화반응에의하여 진행되었으며 두 종류의 반응은 모두 0차반응의 특성을 보였고 탄화수소류, CO+{{{{ { CO}_{2 } }}}} 및 수소가스의 생성속도식을 얻었다. 유중가스 분석과 UV-Visible 분광광도법에 의하여 변압기내의 철심과 동코일이 열화과정에서 촉매역활을 함을 확인하였다.