• Steel and Composite Structures
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• v.24 no.3
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• pp.383-391
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• 2017

The goal of this study is to investigate structural analysis and behaviors of an innovative aerial work platform truss frame whose ductility is improved by using high strength-steel UL-700. The present space truss frame can move or stop through tunnels for maintenance constructions by automatic facilities and workmanship within standardized limited building lines of tunnel. Most of all, this method overcomes problematic, which is to block cars during construction periods, seriously, of typical methods like as using truck and scaffolds for tunnel maintenance. According to evaluated appropriate design results of space truss frames of numerical examples by using a commercial MIDAS GEN program, it is verified that design parameters such as layered size, cross-sectional size, and steel material of the present space truss frame are determined to depend on characteristics such as lanes or shape of road tunnels.

• Steel and Composite Structures
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• v.24 no.4
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• pp.513-521
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• 2017

The goal of this study is to investigate structural analysis and behaviors of an innovative aerial work platform truss frame whose ductility is improved by using high strength-steel UL-700. The present space truss frame can move or stop through tunnels for maintenance constructions by automatic facilities and workmanship within standardized limited building lines of tunnel. Most of all, this method overcomes problematic, which is to block cars during construction periods, seriously, of typical methods like as using truck and scaffolds for tunnel maintenance. According to evaluated appropriate design results of space truss frames of numerical examples by using a commercial MIDAS GEN program, it is verified that design parameters such as layered size, cross-sectional size, and steel material of the present space truss frame are determined to depend on characteristics such as lanes or shape of road tunnels.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.1
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• pp.193-206
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• 2013

This study was to provide the base data on the status of vegetations and soils in green roofs by analyzing the soil and vegetation characteristics of 4 green roofs in Dongguk University in September 2012. Sanglokwon(SW), Dongguk Hall(DH), University Library(UL), and Information and Culture Hall P(IC) were established in 2005, 2008, 2009, and 2010, respectively. The areas of green roofs were $700m^2$, $2,300m^2$, $1,240m^2$, and $640m^2$ in SW, DH, UL, and IC respectively. The investigated floras of vascular plants were 26 families, 55 genera, 65 species in Sanglokwon(SW), 53 families, 99 genera, 112 species in Dongguk Hall(DH), 43 families, 77 genera, 84 species in University Library(UL), and 41 families, 71 genera, 75 species in Information and Culture Hall P(IC), respectively. A positive correlation is shown between the number of plant species and planting area. Total nitrogen, organic matter, and potassium in soil have positive correlation with the number of plant species. The number of plant species was proportional to area and increased more than twice after planting. About a quarter of the invaded plants (including native and naturalized species) were naturalized plants. The total soil depths including vegetation soil and drainage soil at SW, DH, UL, and IC were 20cm, 10cm, 10cm, and 8cm, respectively. The depths of vegetation soil at SW, DH, UL, and IC were <7cm, <3cm, <2cm, and <2cm respectively. The soil pH in vegetation soil ranged from 5.22 to 5.36, and from 6.13 to 6.39 in drainage soil. Available-P concentration ranged from 10.17 to 189.77mg/kg in vegetation soil and from 6.70 to 81.17mg/kg in drainage soil. Carbon concentration in vegetation soil ranged from 2.93 to 9.70%, and 2.93 to 9.70% in drainage soil. Carbon contents in 20cm, 10cm, 10cm, and 8cm soil depths were $2.62kg/m^2$, $1.89kg/m^2$, $0.50kg/m^2$, and $0.53kg/m^2$ at SW, DH, UL, and IC, respectively.

• Smart Structures and Systems
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• v.15 no.4
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• pp.949-961
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• 2015

The goal of this study is to present numerical modeling and analytical testing in order to evaluate an innovative space truss typed temporary structure, which is used to maintenance and repair of road tunnels. The present space truss structure has merits to use UL-700 high strength steel tube as members and to carry out maintenance and repair works of road tunnels without blocking cars and transportations. Numerical modeling and analytical testing of the space truss are investigated by using commercial engineering software, i.e., ABAQUS 6.5-1, and then it is verified that the truss structure has both structural safety and effective function for maintenances and repairs of road tunnels.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.61.3-62
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• 2015

Remote-sensing observation is one of the observation methods that provide valuable information, such as composition and surface physical conditions of solar system objects. The Hayabusa spacecraft succeeded in the first sample returning from a near-Earth asteroid, (25143) Itokawa. It has established a ground truth technique to connect between ordinary chondrite meteorites and S-type asteroids. One of the scientific observation instruments that Hayabusa carried, Asteroid Multi-band Imaging Camera(AMICA) has seven optical-near infrared filters (ul, b, v, w, x, p, and zs), taking more than 1400 images of Itokawa during the rendezvous phase. The reflectance of planetary body can provide valuable information of the surface properties, such as the optical aspect of asteroid surface at near zero phase angle (i.e. Sun-asteroid-observer's angle is nearly zero), light scattering on the surface, and surface roughness. However, only little information of the phase angle dependences of the reflectance of the asteroid is known so far. In this study, we investigated the phase angle dependences of Itokawa's surface to understand the surface properties in the solar phase angle of $0^{\circ}-40^{\circ}$ using AMICA images. About 700 images at the Hayabusa rendezvous phase were used for this study. In addition, we compared our result with those of several photometry models, Minnaert model, Lommel-Seeliger model, and Hapke model. At this conference, we focus on the AMICA's v-band data to compare with previous ground-based observation researches.

• 한국해양학회지
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• v.30 no.5
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• pp.458-466
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• 1995

To clarify the role of protozooplankton in the microbial food web of Inchon coastal ecosystem, the grazing capacities and growth rates of heterotrophic flagellated (HFL) and ciliates were investigated. We measured the growth rates of bacteria, HFL, autotrophic flagellated (AFL) and ciliates using reverse flow (RF) filter set and obtained the clearance rates and ingestion rates of HFL and dilates by fluorescent labelled bacteria (FLB) method and isolation culture method from natural seawater of Inchon coastal area. The standing crops of bacteria, HFL, AFL and ciliates in this study area were 0.7∼1.5${\times}$10/SUP 6/ cells$.$ml/SUP -1/ 1,680∼2,180 cells$.$ml/SUP -1/, 1,090∼2,180 cells$.$ml, and 3,600∼5,700 cells$.$l/SUP -1/, respectively. The mean growth rates of bacteria, HFL, AFL and ciliates were 0.039 h/SUP -1/, 0.032∼0.033h/SUP -1/, 0.015∼ 0.025h/SUP -1/and 0.021h/SUP -1/, respectively. The growth rates of protozooplankton of Inchon coastal waters were relatively low. Mean grazing rate of HFL, AFL and ciliates were0.039 h/SUP -1/, 0.032∼0.033h/SUP -1/,0.015∼ 0.025h/SUP -1/and 0.021h/SUP -1/, respectively. The growth rates of protozooplankton of Inchon coastal waters were relatively low. Mean grazing rate of HFL on bacteria was 0.055h/SUP -1/. Mean grazing rates of ciliates on HFL was relatively high of 0.057 h/SUP -1/, while the grazing rate of ciliates on AFL was low of 0.019h/SUP -1/. The clearance rates and ingestion rates of HFL on bacteria were 3.6∼61.8nl$.$HFL$.$h/SUP -1/ and 6∼52 BAC. HFL$.$h/SUP -1/ respectively. FLB grazing experiment showed low values, while natural bacteria experiment showed high value meaning high grazing pressure on total bacteria. The clearance rates and ingestion rates of Tintinnopsis sp., dominant ciliates in Inchon coastal waters, were 3.2∼4.6 ul$.$CIL/SUP -1/$.$h/SUP -1/ and 5∼6 cells$.$CIL/SUP -1/$.$h/SUP -1/ respectively, showing higher values in the high tide than low tide. The ingestion rates of ciliates implies that ciliate took up 17∼20% of total nanoflagellates of Inchon coastal waters.

• The Korean Journal of Pesticide Science
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• v.18 no.3
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• pp.202-209
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• 2014

RNA interference (RNAi) is the method which controls phenotypes of gene in live cells. Chitinase is the enzyme helping digestion and absorption of old cuticles during the ecdysis of insects. In order to investigate molting-inhibition effect with the chitinase related gene in Spodoptera litura, RNA was extracted from the $5^{th}$ instars. cDNA was synthesized and then we obtained about 700 bp size chitinase. After PCR products were cloned into a pGEM T-easy vector, colonies were picked. DNA was extracted from the colony cultures. EcoR I enzyme was used to check whether PCR products were inserted or not. And then we confirmed vector band of about 3 kb and insert band of about 700 bp. To synthesize the dsRNA, each DNA was cut with Spe I and Nco I enzymes (Circular DNA became lineared DNA). After synthesis of dsRNA, approximately 5 ul dsRNA was injected into the $3^{rd}$ abdominal segment of S. litura $4^{th}$ larvae. The concentration of dsRNA was about $10{\mu}g/{\mu}l$. We confirmed larval-larval molting : there were phenotypically abnormal individuals - for instance malformation, molting inhibition and change of integument color. Pupaadult molting : there were phenotypically abnormal individuals - for instance molting inhibition, change of wings and malformation. Also we could investigate the pupation, emergence and variation about noninjection, treated with DW and dsRNA. Each pupation was non-injection 83.3%, DW 78.3% and dsRNA 66.7%. Each emergence was non-injection 90.0%, DW 72.3% and dsRNA 65.0%. So we considered that chitinase dsRNA induced molting inhibition effect. But each variation was non-injection 8.9%, DW 2.9% and dsRNA 19.2%. Therefore dsRNA group showed the highest variation value. When 18 hours after injecting dsRNA, we could obtain abnormal individual.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.84-84
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• 2003

DNA methylation is involved in epigenetic processes such as X-chromosome inactivation, imprinting and silencing of transposons. DNA methylation is a highly plastic and critical component of mammalian development The DNA methyltransferases (Dnmts) are responsible for the generation of genomic methylation patterns, which lead to transcriptional silencing. The maintenance DNA methyltransferase enzyme, Dnmt 1, and the de novo methyltransferase, Dnmt3a and Dnmt3b, are indispensable for development because mice homozygous for the targeted disruption of any of these genes are not viable. The occurrence of DNA methylation is not random, and it can result in gene silencing The mechanisms underlying these processes are poorly understood. It is well established that DNA methylation and histone deacetylation operate along a common mechanistic pathway to repress transcription through the action of methyl-binding domain proteins (MBDs), which are components of, or recruit, histone deacetylase (HDAC) complexes to methylated DNA. As a basis for future studies on the role of the DNA-methyl-transferase in porcine development, we have isolated and characterized a partial cDNA coding for the porcine Dnmt1. Total RNA of testis, lung and ovary was isolated with TRlzol according to the manufacture's specifications. 5 ug of total RNA was reverse transcribed with Super Script II in the presence of porcine Dnmt 1 specific primers. Standard PCRs were performed in a total volume of 50 ul with cDNA as template. Two DNA fragmenets in different position were produced about 700bp, 1500bp and were cloned into pCR II-TOPO according to the manufacture's specification. Assembly of all sequences resulted in a cDNA from 158bp of 5'to 4861bp of 3'compare with the known human maintenance methyltransferase. Now, we are cloning the unknown Dnmt 1 region by 5'-RACE method and expression of Dnmt 1 in tissues from adult porcine animals.