• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.6
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• pp.599-602
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• 2005

Truncated form of UBP1, an ubiquitin-specific protease of Saccharomyces cerevisiae, was overexpressed in Escherichia coli. The hexahistidine residue $(His_6)$ was fused to the N-terminus of truncated UBP1 and the corresponding recombinant protein was purified with high yield by immobilized metal affinity chromatography. The truncated form of UBP1 protein was functional to cleave ubiquitinated human growth hormone as substrate. Effects of pH and temperature were investigated in order to optimize deubiquitinating reactions for the truncated UBP1. Optimum temperature and pH for the cleavage reaction were $40^{\circ}C$ and pH 8.0, respectively.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.554-556
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• 2003

This research was focused on expression, purification and characterization of ubiquitin-specific protease 1 (UBP1) expressed in recombinant Escherichia coli. Various systems were constructed by fusing polycationic fusion tails or fusion partners to the C- or N-terminus of the product protein. In particular, UBP1 containing 6 histidine residues at the N-terminal end showed best results in terms of expression level and purification efficiency. The N-terminal $6{\times}His-tagged$ UBP1 was overproduced in recombinant E. coli using high cell density cultivation technology and purified using immobilized metal affinity chromatography. The molecular weight of UBP1 was found to be 83,500 daltons. The optimum temperature and pH for the enzyme reaction when ubiquitin-human growth hormone (hGH) was used as a substrate were $40^{\circ}C$ and pH 8.0, respectively.

• International Journal of Control, Automation, and Systems
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• v.3 no.1
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• pp.15-31
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• 2005

Nowadays, the road safety is an important research field. One of the principal research topics in this field is the vehicle localization in the road network. This article presents an approach of multi sensor fusion able to locate a vehicle with a decimeter precision. The different informations used in this method come from the following sensors: a low cost GPS, a numeric camera, an odometer and a steer angle sensor. Taking into account a complete model of errors on GPS data (bias on position and nonwhite errors) as well as the data provided by an original approach coupling a vision algorithm with a precise numerical map allow us to get this precision.

• Journal of the Korea Organic Resources Recycling Association
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• v.16 no.2
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• pp.81-87
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• 2008

We have investigated the efficacy and efficiency of the used briquets for the sewage treatment in this study. The effluent of wastewater treated by Used Briquets Process (UBP) showed very low values of $BOD_5$, CODcr, and SS (1.1 mg/L, 9.5 mg/L, and 3.8mg/L, respectively), which corresponded to 99%, 92.7%, and 96.3% of removal efficiency to the raw wastewater. The number of total bacteria and E. coli in the effluent water treated by UBP was found to be 9 and 1.2 cfu/ml, respectively, showing the remarkable decrease in number, when compared to that of other sewage treatment plants. In the long period of experiment over 1 year, UBP has also shown the high removal efficiency with less sludge production, and low seasonal fluctuation of the values. This UBP is very environmentally-friendly process in the aspects of recycling used briquets and reducing the sludge production. We think that this process will be useful for both the small-scale sewage treatment and reuse of the effluent water.

• Journal of Korea Foresty Energy
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• v.19 no.1
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• pp.13-19
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• 2000

This study was aimed to investigate the effect of several stimulatory trements of flower and seed growth on the reproductive and vegetative growth of Tilia amurensis RUBP. The effects of mounded planting, girdling, root-pruning, bending and foliar fertilizer application on the fifteen to forty yead-old tress growing in the experimental plantation at the Konkuk University and the National forest at the Daegwanryung were measured. The following results were obtained: (1) The number and the longitydinal growth of internode were reduced by the treatments. The xylem growth of the longitudited more greatly than the bark growth by all the treatments. (2)The increasong rate of the brand angle as compared to that of the control was 2.11% in the girdling treatment, 31.61% in the mounded planting treatment, and 22.14% in root-pruning treatment. (3) The foliar application of fertilizer(N, P, K, and B) before flowering increased leaf and stem growth, that one after pollen fertilization increased the fruit growth. (4)The foliar application of fertilizer before flowering increased the xylem growth while that after pollen fertilization increased the bark growth. (5)The stimulatory effects of root pruning and branch bending on the flowering and seed growth were shown relatively less than that of mounded planting. The effect of the moinded planting on the yield of the seeds has remained continuously every year since planting the trees.

• Korean Chemical Engineering Research
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• v.55 no.3
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• pp.369-378
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• 2017

Vascular endotherial growth factor (VEGF) is a potent mitogen that stimulates vascular permeability and angiogenesis and has a potential in therapeutic applications. An industrial production method that provides high yield as well as purity is needed. Researches for various factors of mild solubilization with combination of ubiquitin fusion protein to increase solubility were carried out as well as by changing pH and denaturant concentration. Usage of pET28-a bacteral expression vector in BL21 (DE3) host cell was capable of producing approximately 14 g/L VEGF fusion protein in 20L fermentor. A purification process consisting of four chromatography steps including refolding and digestion with UBP1 resulted in mild solublization under the conditions of 2M urea and pH 10.0 due to ubiquitin fusion tag protein that increases in solubility of target protein VEGF. High yield of refolding and dimerization could be obtained between two step Ni-affinity chromatography. Multimeric and misfolded proteins and endotoxin were removed by DEAE anion exchange chromatography. Final monomers were removed from dimers by gel filtration chromatography. Characterization analysis of purified dimeric VEGF was performed using SDS-PAGE and RP-HPLC with a purity of 97%.