• Title/Summary/Keyword: U1chin

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Separation Performance and Application of Sericin Protein in Silk Degumming Solution(2) (실크 정련 세리신 단백질의 분리특성과 응용(2))

  • Cha, Chin-U;Park, In-Woo;Bae, Kie-Seo;Hong, Young-Ki;Lee, Seo-Hee;Kim, Yong-Duck
    • Textile Coloration and Finishing
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    • v.22 no.2
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    • pp.132-139
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    • 2010
  • Sericin pulverization process was applied by freezing-thawing of sericin protein concentration solution and physicochemical properties of sericin/chitosan blended films were investigated. In sericin pulverization process by freezing-thawing method, the refrigeration storage at $4^{\circ}C$ maximized gelling between sericin molecules, which increased 10% of recovery ratio from sericin concentration solution that using ultrafiltration procedure. In physicochemical properties of sericin/chitosan blended films, the maximum load of chitosan (6.7kgf) had higher than that of sericin (1.2kgf), and the elongation of sericin and chitosan had 96% and 34%, respectively. Also FT-IR analysis of sericin/chitosan blended films showed that both sericin and chitosan films had amide I peak (N-H bond) in $1,521cm^{-1}$ and amide II peak (C=O bond) in $1,630cm^{-1}$. In addition, it could confirm compatibility between both materials as indicated by the decrease in the amide I peak's absorption value as chitosan content increases.

The Improvement and Completion of Outcome index: A new assessment system for quality of orthodontic treatment

  • Hong, Mihee;Kook, Yoon-Ah;Kim, Myeng-Ki;Lee, Jae-Il;Kim, Hong-Gee;Baek, Seung-Hak
    • The korean journal of orthodontics
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    • v.46 no.4
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    • pp.199-211
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    • 2016
  • Objective: Given the considerable disagreement between the Peer Assessment Rating (PAR) index and the American Board of Orthodontics Cast-Radiograph Evaluation, we aimed to develop a novel assessment system-the Improvement and Completion of Outcome (ICO) index-to evaluate the outcome of orthodontic treatment. Methods: Sixteen criteria from 4 major categories were established to represent the pretreatment malocclusion status, as well as the degree of improvement and level of completion of outcome during/after treatment: dental relationship (arch length discrepancy, irregularity, U1-SN, and IMPA); anteroposterior relationship (overjet, right and left molar position, ANB); vertical relationship (anterior overbite, anterior open-bite, lateral open-bite, SN-MP); and transverse relationship (dental midline discrepancy, chin point deviation, posterior cross-bite, occlusal plane cant). The score for each criterion was defined from 0 or -1 (worst) to 5 (ideal value or normal occlusion) in gradations of 1. The sum of the scores in each category indicates the area and extent of the problems. Improvement and completion percentages were estimated based on the pre- and post-treatment total scores and the maximum total score. If the completion percentage exceeded 80%, treatment outcome was considered successful. Results: Two cases, Class I malocclusion and skeletal Class III malocclusion, are presented to represent the assessment procedure using the ICO index. The difference in the level of improvement and completion of treatment outcome can be clearly explained by using 2 percentage values. Conclusions: Thus, the ICO index enables the evaluation of the quality of orthodontic treatment objectively and consecutively throughout the entire treatment process.

A Study on the Alkaline Protease Produced from Bacillus subtilis (Bacillus subtilis가 생산하는 Alkaline Protease에 관한 연구)

  • Chang, Shin-Jae;Kim, Yoon-Sook;Sung, Ha-Chin;Choi, Yong-Jin;Yang, Han-Chul
    • Applied Biological Chemistry
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    • v.31 no.4
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    • pp.356-360
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    • 1988
  • The alkaline protease producing bacteria isolated from soil and identified as Bacillus subtilis. The optimum medium for alkaline protease production from the microorganism was as follows; soluble starch, 1.5% ; proteose peptone, 0.5% ; $K_2HPO_4$, 0.1% ; $MgSO_4{\cdot}7H_2O$, 0.02% and sodium carbonate, 1.0%. The optimum temperature for alkaline protease production was $35^{\circ}C$, and the initial pH of medium was pH 10.5. The alkaline protease activity was about 2,300 U per ml of culture broth by Casein-Folin Method. A 9.2 fold purification of alkaline protease was obtained from culture broth. The recovery was 14% and purified enzyme was identified as single band, and its molecular weight was about 19,000. The optimum temperature for enzyme reaction was $70^{\circ}C$, and optimum pH was 12. The activity of purified enzyme was inhibited by metal ion ($Fe^{++}$), and Phenylmethylsulfonyl Fluoride, a serine protease inhibitor.

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Analysis on the Pigment Composition of Phytoplankton Assemblages using HPLC (High Performance Liquid Chromatography) in the Adjacent Waters of Nuclear Power Plants in Spring

  • Choi, Hyu-Chang;Kang, Yeon-Shik;Choi, Joong-Ki;Song, Tae-Yoon;Yoo, Man-Ho
    • Journal of the korean society of oceanography
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    • v.39 no.4
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    • pp.234-242
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    • 2004
  • The pigment composition and concentration of phytoplankton assemblages using HPLC in the adjacent waters of four nuclear power plants (Yonggwang, Kori, Wolsong and Ulchin) were investigated during the spring blooming in 2004. The mean concentration of chlorophyll a ranged from 563.8 to 2,949.0ng $l^{-1}$, with the lowest concentration at Kori and the highest concentration at Wolsong. Among the carotenoids, the amounts of fucoxanthin and chlorophyll $C_2$ were relatively higher than those of other pigments in the study site. As minor pigments, zeaxanthin, chlorophyll b, 19'-butanoyloxyfucoxanthin, diadinoxanthin, 19'-hexanoyloxyfucoxanthin, chlorophyll $C_3$ and peridinin were detected. The results of pigment composition and concentration showed that diatoms had an important proportion of phytoplankton community when a spring bloom occurred. Cyanobacteria was present relatively low density at the Wolsong and the green alga such as chlorophytes and prasinophytes were abundant at the Yonggwang and Kori, while dinoflagellates characterized by peridinin were common at Ulchin and Kori. The pigment composition and concentration of phytoplankton after passing through the cooling-water system of nuclear power plant were highly variable. No distinct trend of the change of each pigment composition and amount was detected but the variation of fucoxanthin and chlorophyll $C_2$ highly coupled with that of chlorophyll a. We pointed out that the diatom controlled the overall variation of phytoplankton biomass during the spring season.

An Optimization Algorithm of Gypsum Board Loss for Wall Finishing in Modular Construction System (모듈러건축 벽체마감 석고보드 손실 최적화 알고리즘 개발)

  • Lee, Dong-Min;Chin, Sangyoon
    • Korean Journal of Construction Engineering and Management
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    • v.15 no.1
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    • pp.101-110
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    • 2014
  • The ministry of Land, Infrastructure and Transport announced that they launched the pilot project to offer Happy House built with modular construction as part of the major projects of incumbent government in 2013. The market size of modular construction is getting increasingly enlarged together with strong will of government. The major challenges that current modular construction encounters can be summed up as lack of standardization of material on the stage of design and fabrication. The portion of material costs in modular construction marks 16 to 17% higher than the existing other construction method, and account for 60 % of total construction cost, which is why material management is the most important factor. However, the imperfect standardization and specification on design causes high loss of materials on fabrication, which makes the construction wastes and total construction cost increasing in accordance with the increase of material costs. This study has been conducted to verify major modules by developing optimization algorithm on gypsum board material among wall finishing materials. It is expected that this paper contributes not only to eco-friendly construction by minimizing the waste factors of materials through these efforts, but also to removing high cost issues which had been recognized as a setback of current modular construction.

Effects Of $Interferon-{\gamma}$ On The Biological Activity Of Mouse Osteoblast MC3T3/E1 Cells In Culture (($Interferon-{\gamma}$)가 마우스 조골세포의 생물학적 활성에 미치는 영향에 관한 연구)

  • Lee, Kwan-Hoon;Kim, Jung-Geun;Chung, Chin-Hyung
    • Journal of Periodontal and Implant Science
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    • v.26 no.1
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    • pp.216-229
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    • 1996
  • Interferon(IFN) is a sort of glycoproteins that are produced by activated lymphocyte, monocyte and fibroblast. IFN has anti-viral effects, immuno-defensive mechanism and regulating properties to the several kinds of cells that includes affect on the bone formation and resorption. The effect of IFN on the osteoclast & other tissue cells has been studied in a number of researchers with the limited reports on the osteoblast. The purpose of this study was to evaluate the effects of IFN on the osteoblastic function. The MC3T3/El cell(Mouse osteoblast) was incubated in ${\alpha}-minimum$ essential medium containing 10% FBS. To detect the cytotoxic effect of $IFN-{\gamma}$ on osteoblast, the cells were cultured in 96-well plate to which $IFN-{\gamma}$ of various concentrations were added for 2 days. After staining with trypan blue, total cells and living cells were counted under microscope. To determine the activity of alkaline phosphataset(ALP), various concentrations of $IFN-{\gamma}$ were treated to culture medium, and biochemical assay was performed. $IFN-{\gamma}$ and $IFN-{\gamma}$ plus cycloheximide were added to culture medium separately and then ALP activity were determined. To detect the effect of the $IFN-{\gamma}$ on the bone formation of osteoblast, long-term culture was performed, and calcified nodule formation were observed using von Kossa's staining. After the addition of $IFN-{\gamma}$ with various concentrations to the medium, no cytotoxic effect of $IFN-{\gamma}$ was detected at any concentration. The significant increase in ALP activity of osteoblast were found the concentration of $IFN-{\gamma}$ 500-2500U/ml and the culture time of 24-48 hours respectively. The enhancement of ALP activity by $IFN-{\gamma}$ of osteoblast was decreased significantly by the treatment of cycloheximide. After long-term culture of osteoblast, the nodule formation was found to be increased in number and density by the addition of 500 U/ml $IFN-{\gamma}$. These results suggest that $IFN-{\gamma}$ was affected on the bone formation of osteoblast. Forthemore this kind of study or $IFN-{\gamma}$ to osteoblast will be held continuously.

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Comparison of Nutrient Intakes of Elderly Korean and American Women Using Diet Quality Index

  • Chung, Chin-Eun;Sungsoo Cho
    • Nutritional Sciences
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    • v.2 no.2
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    • pp.107-112
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    • 1999
  • Diet quality index DQI) offers a new way of comparing eating habits across populations and across countries. Nutrients and food consumption data from 100 elderly Korean women aged 65 and older were collected in Seoul or Kyunggi-do, Korea by the 24 hour recall method. Diet quality index (DQI) was computed for 1049 elderly women (65 and older) from the 1994-1996 Continuing Survey of Food Intakes by Individuals (CSFII) in the US according to US dietary guidelines, and applied to the diet of elderly Korean women for purposes of comparison. A modified 16-point DQI awarded 2 points each for moderate consumption of fat ($\leq$ 30% energy), saturated fat ($\leq$ 10%), cholesterol ($\leq$ 300 mg/day), sodium ($\leq$ 2400 mg/day), and protein ($\leq$ 100% RDA), adequate intakes of carbohydrate ($\qeq$50% energy) and calcium ($\qeq$ 100% RDA), and plenty of fruits and vegetables ($\qeq$ 5 servings). Criteria were based on US dietary guidelines. Partial scores were given if subjects were close to meeting these cutoff points. Diets with $\leq$ 300 mg cholesterol/day were reported by 97% or the Korean sample and 82% of the American sample, while 90% of the Koreans and 42% of the Americans met the goal of $\leq$ 30% of energy from fat, and 98% of the Koreans and 47% of American met the recommendation of $\leq$ 10% of energy from saturated fat. In contrast, only 8% of the Korean sample met the sodium recommendation of $\leq$ 2400 mg sodium per day, whereas 54% of the American subjects met this goal. The mean DQI scores were 10.1 for the elderly American women and 11.3 for the elderly Korean women. Overall, the elderly Korean diet was more consistent with the US dietary guidelines than the elderly American diet.

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ROLE OF NF${\kappa}B$ IN TOLL-LIKE RECEPTOR 9-MEDIATED MATRIX METALLOPROTEINASE-9 EXPRESSION (Toll-like receptor 9-매개에 의한 matrix metalloproteinase-9 발현에서 NF${\kappa}B$의 역할)

  • Lee, Sang-Hoon;Chin, Byung-Rho;Baek, Suk-Hwan
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.33 no.6
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    • pp.636-642
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    • 2007
  • Background: CpG DNA plays an important role in immune cell function. This study examined whether the temporal control of toll-like receptor (TLR)9 by CpG DNA can regulate the expression of matrix metalloproteinase-9(MMP-9). Methods and materials: Macrophages were cultured in the presence of 10% FBS. For the various MMP genes analysis, RT-PCR and real-time PCR were performed. In addition, zymography assay performed for the MMP activity. The phosphorylation assay did for the ERK1/2 and NF${\kappa}B$ activation, and luciferase promoter assay was for the NF${\kappa}B$ activity. Results: CpG DNA induced the mRNA expression of MMP-2, MMP-9, and MMP-13, but not of MMP-7, MMP-8, and MMP-12, in a time-dependent manner. Especially, the mRNA expression of MMP-9 was strongly induced by CpG DNA using real-time RT-PCR. The TLR9 inhibitor, chloroquine, suppressed CpG DNA-induced MMP-9 expression and its activity. Moreover, CpG DNA induced the phosphorylation of ERK and the inhibition of ERK by U0126 suppressed CpG DNA-induced MMP-9 expression and its activity. CpG DNA stimulated $I{\kappa}B-{\alpha}$ degradation and luciferase activity. In addition, pretreatment of SN-50, the inhibitor of NF${\kappa}B$, strongly blocked the CpG DNA-induced MMP-9 expression and activity. Conclusion: These observations suggest that CpG DNA may play important roles in the activation of macrophages by regulating the production of MMP-9 via the sequential TLR9-ERK-NF${\kappa}B$ signaling pathway.

COMPASIRON OF BEHAVIOR PROBLEMS AMONG CHILDREN OF KOREAN AND CHINESE ANCESTRY IN YUNBYUN REGION (중국 연변지구 한족과 조선민족 아동행동문제 비교분석)

  • Kim, Pong-Chin;Kim, Chul-Koo;Pyo, Mi-Ja;Choi, Soon
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.2 no.1
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    • pp.150-154
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    • 1991
  • 1012 Yunbyun children (500 of chinese and 512 of Korean ancestry) in kindergarten through sophomore in highschool were assessed using CBCL parental form and their data were compared to study differences in behavior problems among children of korean and chinese ancestry in Yunbyun. The results indicated that social withdrawal, depressive, somatic complaints and aggressive syndromes were fairey common among Yunbyun children of both korean and chinese ancestry and that compared to children in the u. s. and shanghai. Yunbyun children showed more social withdrawal but less aggressive behaviors. Internalizing syndromes such as uncommunicative, schizoid, obsessive and anxious syndromes were more prevalent among children of chinese ancestry, while hyperactive and aggressive syndromes were more prevalent among children of korean ancestry.

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Geochemical Data Analysis of the Granitic Rocks Potentially Related to Fluorite Mineralization in the Geumsan District (금산지역 형석광화작용과 관련된 화강암질암의 지구화학적 자료 해석)

  • Chin, Ho-Ill;Chon, Hyo-Taek;Min, Kyoung-Won
    • Economic and Environmental Geology
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    • v.28 no.4
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    • pp.369-379
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    • 1995
  • About forty ore deposits of $CaF_2{\pm}Au{\pm}Ag{\pm}Cu{\pm}Pb{\pm}Zn$ are widely distributed in the Geumsan district and are believed to be genetically related to the Mesozoic Geumsan granitic rocks. Based on their petrogeochemistry and isotopic dating data, the granitic rocks in this district can be classified into two groups ; the Jurassic granitic rocks(equigranular leucocratic granite, porphyritic biotite granite, porphyritic pink-feldspar granite, seriate leucocratic granite) and the Cretaceous granitic rocks(seriate pink-feldspar granite, equigranular alkali-feldspar granite, equigranular pink-feldspar granite, miarolitic pink-feldspar granite, equigranular biotite granite). Spatial distribution of fluorite ore deposits, fluorine contents of granitic rocks and fracture patterns in this district suggest that three granitic rocks(equigranular biotite granite, equigranular pink-feldspar granite, miarolitic pink-feldspar granite) of the Cretaceous period be genetically related to the fluorite mineralization. In these fluorite-related granitic rocks, fluorine is most highly correlated with Cs(correlation coefficient(r)>0.9), and also highly with MnO, U, Sm, Yb, Lu, Zn, Y, Li(r>0.7). Statistically the variation of fluorine in the fluorite-related granitic rocks can be explained in terros of only three elements, such as Lu, CaO and Cs, and the fluorite-related granitic rocks can be discriminated from the fluorite-nonrelated granitic rocks by a linear functional equation of La, Ce, Cs and F($Z_{Ust}=-1.38341-0.00231F-0.19878Ce+0.38169La+0.54720Cs$). Also, equigranular alkali-feldspar granite is classified into the fluorite-related granitic rocks by means of the linear functional equation($Z_{Ust}$).

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