• Journal of Microbiology and Biotechnology
• /
• v.24 no.4
• /
• pp.431-439
• /
• 2014

We report the construction of two Bacillus subtilis expression vectors, pBNS1/pBNS2. Both vectors are based on the strong promoter P43 and the ampicillin resistance gene expression cassette. Additionally, a fragment with the Shine-Dalgarno sequence and a multiple cloning site (BamHI, SalI, SacI, XhoI, PstI, SphI) were inserted. The coding region for the amyQ (encoding an amylase) signal peptide was fused to the promoter P43 of pBNS1 to construct the secreted expression vector pBNS2. The applicability of vectors was tested by first generating the expression vectors pBNS1-GFP/pBNS2-GFP and then detecting for green fluorescent protein gene expression. Next, the mannanase gene from B. pumilus Nsic-2 was fused to vector pBNS2 and we measured the mannanase activity in the supernatant. The mannanase total enzyme activity was 8.65 U/ml, which was 6 times higher than that of the parent strain. Our work provides a feasible way to achieve an effective transformation system for gene expression in B. subtilis and is the first report to achieve B. pumilus mannanase secretory expression in B. subtilis.

• Korean Journal of Materials Research
• /
• v.5 no.5
• /
• pp.583-597
• /
• 1995

We investigated the effect of the oxygne partial pressure on the phase stability of B $i_{2}$S $r_{2}$Ca C $u_{2}$ $O_{8+x}$ and B $i_{2}$S $r_{2}$C $a_{2}$C $u_{3}$ $O_{10+x}$ at 82$0^{\circ}C$. As the oxygen pressure decreased, B $i_{2}$Sr/sb 2/CaC $u_{2}$ $O_{8+x}$ melted at 2.2$\times$10$^{-3}$ atm $O_{2}$. In the case of the B $i_{1.7}$P $b_{0.4}$S $r_{2}$C $a_{2}$ $O_{10+x}$, it started to decompose into theree phases of B $i_{2}$S $r_{2}$Cu $O_{6+y}$, $Ca_{2}$Cu $O_{3}$ and C $u_{4}$ $O_{3}$ and C $u_{4}$ $O_{3}$ at 8.0$\times$10$^{-3}$ atm $O_{2}$ and was completely decomposed at 4.3$\times$10$^{-3}$ atm $O_{2}$ B $i_{2}$S $r_{2}$C $a_{2}$C $u_{3}$ $O_{10+x}$ phase was not formed by the solid state reaction from the mixutre of $i_{2}$S $r_{2}$CaCu.sub 2/ $O_{8+x}$, $Ca_{2}$Cu $O_{3}$ and CuO down to 2.2$\times$10.sub -3/ atm O.sub 2/ but formed by the solidifciation of the formed from the mixture of the intermediate compounds in the Bi-Sr-Ca-Cu-O system and the fromation temperature of Bi.sub 2/S $r_{2}$C $a_{2}$Cu.$_{3}$ $O_{10+x}$ can be lowered by reducing oxygen partial pressure.e.e.ure.e.e.

• Journal of the Korean Chemical Society
• /
• v.26 no.3
• /
• pp.160-164
• /
• 1982

A guanidinium salt of $[U(PW_{11}O_{39})_2]^{10-}$, the solubility of which is adequate for crystal growing, has been synthesized. Using this salt or potassium salt, we have measured the stability of $[U(PW_{11}O_{39})_2]^{10-}$as a function of pH of the solution and found that the anion is stable for the pH range 3~7. We have developed a colorimetric method for determining the concentration of $U^{4+}$. In this method$PW_{11}O_{39}^{7-}$ is added to$U^{4+}$ in such a quantity that the mole ratio $PW_{11}O_{39}^{7-}/ U^{4+}$exceeds 2 and the intensity of the 22.7kK band (${\varepsilon}$1030 M-1cm-1) is measured. In order to develop a continuous method to recover uranium, we have determined the amount of recoverd$PW_{11}O_{39}^{7-}$ after decomposing $[U(PW_{11}O_{39})_2]^{10}$- by adding either a base or an oxidizing agent. The percentage of $PW_{11}O_{39}^{7-}$recovered was approximately 70% when a base was used and approximately 80% when$K_2S_2O_8$ was used. A colorimetric method for determining $PW_{11}O_{39}^{7-}$ has also been developed.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.25 no.5
• /
• pp.925-932
• /
• 2001

The influence of ultraviolet(UV)-ray in sun light on human skin has been noted. Textiles can provide protection against harmful UV-radiation. Normally UV-absorbing finishes are used to get better protection. The purpose of this study is to evaluate the UV-cut properties of cotton fabrics treated with UV-absorber. 2,2-dihydroxy-4,4-dimethoxbenzophenone, as UV-absorber was applied to 100% cotton fabric. Reagents added in finishing solution were Triton X-100, polyethylene glycol 400, and $MgCl_2{\cdot}6H_2O$, and C.I. Direct Red 81. Both untreated and treated cotton fabrics were exposed to a xenon arc lamp for 20 and 80 hours. UV absorption spectra of finishing solutions and UV transmission spectra of fabrics were measured by the UV/VIS spectrophotometer. The results of this study can be summarized as follows. The results of this study can be summarized as follows. Absorption and the related transmission spectra were modified in a controlled way with UV-absorber. Absorption effect of UV-absorber was improved by adding Triton X-100, PEG 400, and $MgCl_2{\cdot}6H_2O$ in finishing solution. The UV absorption of finishing solution was in the following order: U/D/T/P/M>D/T/P/M> D/T> D/P, D>U/T/P/M>U/T>T/P/M>T. The UV transmittance of cotton fabrics was remarkably decreased by the application of UV-absorber and additives. The UV-cut properties were most improved by the application of U/D/T/P/M.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
• /
• v.9 no.1
• /
• pp.38-42
• /
• 1998

It has been reported that Tonsillectomy ＆ Adenoidectomy(T & A) resulted in the change of voice by structural changes directly to the vocal track. We studied the effect of T & A on the voice of patients comparing the pre-operative to the post-operative voice. It was performed using a Computerized Speech Lab(CSL50) which is currently used as a method for voice analysis. Forty-five patients who had T＆A, aging from 3 to 42 years old, took part in studies and wert evaluated for voice changes and the degree of formant changes of four basic vowels, /a/, /i/, /o/, and /u/. They were evaluated pre-operatively and post-operatively one month later using MDVP, CSL program of CSL50. The results obtained were as follows ; In using MDVP, there were some differences between pre-operative and post-operative shimmer measures within the normal range but other acoustic measures(Fo, jitter, NHR) show no significant differences(p>0.05). F3 of /a/ and /o/ were significantly decreased(p<0.05) and F2, F3 of /i/ were increased(p>0.05) in patients who only had Tonsillectomy in doing CSL spectrogram. For the patients who had T & A, Fl and F3 of /a/, F3 of /i/, Fl, F2 and F3 of /o/ were decreased with significant increase in F1 and F2 of /i/(p<0.05).

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2004.07b
• /
• pp.1211-1213
• /
• 2004

In this paper, in other the study shift a degradation and electrical properties on ZnO based grain beurdry layer, we mearured thermally stmulated current. Also the TSC was investigated for understanding of GBL's interfacial carrier shift on bias voltage, bias time, bias temperature. as a result, the two peahs of $p_1$, $p_2$ was observed by conduction of the trapped carrier of border between the oxidation layer and the grains $P_3$ and $P_4$ Peaks observed to the ionization excition excitation in the grain.

• Bulletin of the Korean Mathematical Society
• /
• v.60 no.4
• /
• pp.1085-1100
• /
• 2023

For any given function f, we focus on the so-called prescribed mean curvature problem for the measure e^-f(|x|2)dx provided thate^-f(|x|2) ∈ L¹(ℝⁿ⁺¹). More precisely, we prove that there exists a smooth hypersurface M whose metric is ds² = dρ² + ρ²d𝜉² and whose mean curvature function is ${\frac{1}{n}}(\frac{u^p}{{\rho}^{\beta}})e^{f({\rho}^2)}{\psi}(\xi)$ for any given real constants p, β and functions f and ψ where u and ρ are the support function and radial function of M, respectively. Equivalently, we get the existence of a smooth solution to the following quasilinear equation on the unit sphere 𝕊ⁿ, $${\sum_{i,j}}({{\delta}_{ij}-{\frac{{\rho}_i{\rho}_j}{{\rho}^2+|{\nabla}{\rho}|^2}})(-{\rho}ji+{\frac{2}{{\rho}}}{\rho}j{\rho}i+{\rho}{\delta}_{ji})={\psi}{\frac{{\rho}^{2p+2-n-{\beta}}e^{f({\rho}^2)}}{({\rho}^2+|{\nabla}{\rho}|^2)^{\frac{p}{2}}}}$$ under some conditions. Our proof is based on the powerful method of continuity. In particular, if we take $f(t)={\frac{t}{2}}$, this may be prescribed mean curvature problem in Gauss measure space and it can be seen as an embedded result in Gauss measure space which will be needed in our forthcoming papers on the differential geometric analysis in Gauss measure space, such as Gauss-Bonnet-Chern theorem and its application on positive mass theorem and the Steiner-Weyl type formula, the Plateau problem and so on.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1996.04a
• /
• pp.178-178
• /
• 1996

Hepatitis B virus (HBV) infection is one of the serious problems in Southeast Asia including Korea because it causes chronic hepatitis, which can easily be transformed In fatal conditions such as cirrhosis and hepatoma. Even though lots of informations on structural characteristics and gene expression mechanisms have been accumulated, the mechanism for HBV-induced hepatocellular injury which is believed to be the consequences of the immunological response is not well understood. In order tn perform immunopathological studies for prevention and treatment of HBV infection, we designed transgenic mice as a disease model which can mimic HBV infection, In this study, a promoter-HBV DNA fragment for the preparation of HBV transgenic mice has been constructed. To add a proper enzyme site on 5' end of HBV gene, total HBV (subtype adr) gene was inserted into BamHI site of pBluescript SK vector and reextracted by PstI-SacI treatment A liver-specific promoter, rat ${\alpha}$ 2u globulin gene promoter, was insrted to pBluescript SK vector and reextracted by BamHI-PstI treatment, Promoter-HBV DNA was constructed by ligation of two fragments using identical PstI sites. For large scale production of promoter-HBV DNA, it was inserted to BamHI-SacI site of pBluescript SK vector.

• Journal of the Korean Society for information Management
• /
• v.6 no.1
• /
• pp.3-14
• /
• 1989

The purpose of t h i s paper i s to i n v e s t i g a t e the development of academic magazines and i t s Charac t e r i s t ~ c s i n o r d e r to determine i t s function a s a means of p r e s e n t i n g t h e i r r e s u l t s of reasearch in Korea.

• Journal of Chest Surgery
• /
• v.26 no.6
• /
• pp.427-440
• /
• 1993

The paucity of donor hearts for transplantation can be remedied by distant heart procurement. Prolonging donor heart preservation is essential for successful clinical cardiac transplantation. Thirty-two isolated rat hearts were perfused with Krebs-Henseleit buffer solution for 15 minutes, arrested and preserved at 4 oC for 4 hours, and then reperfused for 25 minutes. The following three groups were prepared and hemodynamic changes, creatine kinase-MB isoenzyme levels and ultrastructural changes of the myocardium were analysed before and after cardiac arrest. ; Group I : the heart was arrested with the cardioplegic solution [Plegisol, potassium : 16 mM, sodium : 120 mM] and then stored in a solution with ionic compositions of the extracellular fluid [Hartman, potassium : 4 mM, sodium : 130 mM] ; Group II : the heart was arrested with the cardioplegic solution and stored in a solution with ionic compositions of the intracellular fluid [Modified Euro-Collins, potassium : 108 mM, sodium : 10 mM] ; Group III : the heart was arrested with the cardioplegic solution containing adenosine 20 uM, and then stored in a solution with ionic compositions of the intracellular fluid [Modified University of Wisconsin solution, potassium : 119 mM, sodium: 23 mM]. Left ventricular developed pressure at 20 minutes of the reperfusion was significantly higher in group III [64.3 $\pm$ 3.12 mmHg, p<0.01] and group II [58.3 $\pm$ 1.55 mmHg, p<0.05] as compared with group I [51.4$\pm$ 2.78 mmHg]. The time to induce cardiac arrest after infusion of cardioplegic solution with adenosine 20 uM [5.3 $\pm$ 0.30 second, p<0.005] was significantly shorter than without adenosine [10.6$\pm$ 0.55 second]. Coronary flow at 20 minutes of the reperfusion was augmented significantly in group III [9.6$\pm$ 0.50 ml/min, p<0.05, p<0.05] as compared with group I [8.0 $\pm$ 0.41 ml/min] and group II [8.1$\pm$ 0.51 ml/min]. Percentage recovery of left ventricular developed pressure at 20 minutes of the reperfusion was significantly higher in group III [94.6$\pm$ 2.51 %, p<0.005] as compared with group II and in group II [83.1 $\pm$ 1.22 %, p<0.005] as compared with group I [69.9 $\pm$ 1.73 %], and also percentage recovery of coronary flow at 20 minutes of the reperfusion was significantly higher in group III [82.3 $\pm$ 3.86 %, p<0.05] as compared with group II [71.4 $\pm$ 3.46 %] but there was no significant difference between group I and group II. Measured level of creatine kinase-MB isoenzyme at 15 minutes of the reperfusion was significantly lower in group III [1.23 $\pm$ 0.16 ng/ml, p<0.025] and group II [1.42$\pm$ 0.10 ng/ml, p<0.05] as compared with group I [1.79 0.14 ng/ml]. In the semiquantitative evaluation of the ultrastructural changes of the myocardium, mitochondrial score was lower in group III [0.7 $\pm$ 0.21] than in group I [3.1$\pm$ 0.28] and group II [1.7 $\pm$ 0.19], and also the other structural score was lower in group III [2.7$\pm$ 0.99] than in group I [7.9 $\pm$ 0.89] and group II [5.0 $\pm$ 1.22]. In conclusion, the solution with ionic compositions of the intracellular fluid is appropriate for prolonged cardiac preservation, and it appears to be better preserving method for distant procurement when the donor heart is rapidly arrested with cardioplegic solution containing adenosine 20 uM, and then stored with Modified University of Wisconsin solution.