• Journal of Nutrition and Health
• /
• 제35권10호
• /
• pp.1053-1059
• /
• 2002

In post-genome period, the technique for identifying gene expression has been changed to high throughput screening. In the field of molecular nutrition, the need for this technique to clarify molecular function of the specific nutrient is essential. In this study, we have tested the zinc-regulated gene expression in zinc-deficient U937 cells, using cDNA microarray which is the cutting-edge technique to screen large numbers of gene expression simultaneously. The study result can be used for the preliminary gene screening data for clarifying, using monocyte U937 cell line, molecular Zn aspect in atherosclerosis. U937 cells were cultured in Zn-adequate (control, 12 $\mu$M Zn) or Zn-deficient (experimental, 0 $\mu$M Zn) ESMI media during 2 days, respectively. Cells were harvested and RNA was extracted. Total RNA was reverse-transcriptinized and synthesized cDNA probe labeled with Cy-3. fluorescent labeled cDNA probe was applied to microarray slide for hybridization slide, and after then, the slide was scanned using fluorescence scanner. ‘Highly expressed genes’ in Zn-deficient U937 cells, comparing to Zn-adequate group, are mainly about the genes for motility protein, immune system protein, oncogene and tumor suppressor and ‘Less highly expressed genes’ are about the genes for transcription, apoptosis associated protein, cell cycle, and several basic transcription factors. The results of this preliminary study imply the effectiveness of cDNA microarray for expression profiling of a singly nutrient deficiency, specially Zn. Furthur study, using tailored-cDNA array and capillary endothelial cell lines, would be beneficial to clarify molecular Zn function, more in detail.

• 재활복지
• /
• 제15권4호
• /
• pp.351-375
• /
• 2011

본 연구의 목적은 u-Learning 학습매체인 IT 기기와 웹 기반의 e-Learning 교육을 서로 호환할 수 있는 IT 직업교육과정을 개발하기 위해 청각장애인의 접근성에 대한 문제점과 개선 방안, 그리고 u-Learning 기반 차세대 IT 학습 매체로서의 교육적 활용 가능성에 대한 기초자료를 제공하는데 그 목적이 있다. 이 연구를 수행하기 위해서 장애인 기관협회의 수화통역사 사전교육을 통하여 경북·대구광역시에 거주하는 100명의 청각장애인을 대상으로 설문조사를 실시하였다. 이 연구를 통해 나타난 결론은 다음과 같다. 첫째, 오프라인 컴퓨터 교육과 웹 기반의 e-Learning 교육에 대한 청각장애인에게 고려되어야 할 요소로는 청각장애인들을 위한 수화 동영상 및 자막 서비스를 제공해 주지 않는 점과 더불어 강의내용에 대한 청각장애인의 의미파악에 어려움으로 인해 학습효과가 낮아 수화통역이 가능한 교수와 꾸준한 상호협력 및 멘토링이 필요하며, 또한 자막의 크기와 색상 및 배치, 수화동영상의 크기와 배치 등의 청각장애인을 위한 사용자환경 설정 및 강의 내용과 자막서비스를 동시에 텍스트를 제공이 중요한 것으로 나타났다. 둘째, u-Learning 학습매체인 IT 기기와 웹 기반의 e-Learning 교육을 서로 호환할 수 있는 IT 직업교육과정 개설 시 수업참여 여부가 74%로 나타나 청각장애인에게 높은 기대감을 보여 IT 직업교육과정 개발이 필요한 것으로 나타났다. 셋째, IT 직업교육과정을 수강하기 위한 선호하는 IT 기기로는 태블릿 PC가 가장 높았으며, IT직업 교육과정의 개설과목으로 IT 관련 자격증 과목개설을 가장 많이 선호하여 취업을 연계하기 위해서 컴퓨터 중급부터 고급과정까지 체계적으로 습득할 수 있는 IT 직업교육과정의 다양한 콘텐츠가 필요할 것이다.

• 대한골관절종양학회지
• /
• 제13권2호
• /
• pp.88-95
• /
• 2007

목적: wild-type p53 단백질을 가지고 있는 U2OS 인간 골육종 세포주의 세포자멸사에서 MMP억제제와 doxorubicin의 작용을 비교하고자 하였다. 대상 및 방법: 배양한U2OS 세포주에 Doxorubicin, MMP억제제(MMPI III)를 따로 투여한 것과 두 약물을 동시에 투여한 것을 비교하였다. 또한 Doxorubicin의 작용이 Fas/FasL 경로를 통해 유발되는지 알아보기 위해 Fas 중화항체를 doxorubicin과 함께 투여하였다. 약물을 처리하여 배양한 세포에서 발생한 세포자멸사와 세포괴사를 확인하기 위해서 유세포 검사를 시행하였다. 결과: Doxorubicin을 처리한 세포가 처리하지 않은 세포보다 약물 농도에서 더 많은 세포괴사(p=0.000)를 보였다. 이에 반해 MMPI III를 처리한 세포는 약물 농도와 반응시간에 따른 세포자멸사 및 세포괴사에서 차이를 보이지 않았다. 두 약물을 동시에 투여한 군은 그렇지 않은 군에 비해 세포 자멸사 및 세포괴사에서 차이가 없었다. Doxorubicin에 Fas 중화항체를 추가한 군과 doxorubicin 단독 투여 군 사이에 세포자멸사 및 세포괴사에서 차이가 없었다. 결론: Doxorubicin과 같이 MMP 억제제를 골육종 치료에 사용하려면 wild-type p53 뿐만 아니라 wild-type p14를 가지고 있는 골육종 세포에 대한 검사가 필요할 것으로 사료된다.

• 생약학회지
• /
• 제30권1호
• /
• pp.7-11
• /
• 1999

The present work was carried out to examine the effect of costunolide on the growth of several cells and characteristics of U-937 human leukemia-derived cell line. Costunolide produced a potent antitumor activity in vitro dependent on concentration against several tumor cells such as P-388, L-1210 leukemia and SNU-5 stomach cancer cells. However, it showed less cytotoxicity on normal cells such as Maccaccus rheus monkey kidney cells (MA-104) up to 200 ${\mu}M$ concentration. An effect of cell differentiation by costunolide was assessed by its ability to reduce nitroblue tetrazolium (NBT), and to induce phagocytosis of latex particles. In order to establish whether costunolide induces U-937 cells to differentiate toward macrophage or granulocyte, esterase activities was measured. Based on these results, we found that costunolide having cytotoxicity on U-937 human leukemia cells was explained through differentiation inducing activity.

• 동의생리병리학회지
• /
• 제20권2호
• /
• pp.358-364
• /
• 2006

To investigate the possible mechanism of Drynariae Rhizoma extracts as a candidate of anti-cancer drug, I examined the effects of Drynariae Rhizoma extracts on the apoptosis of U937 cell line. MTT assay, flow cytometric analysis, SDS-polyacrylamide gel electrophoresis, Western blot analysis, and RT-PCR were performed. Drynariae Rhizoma extracts treatment reduced the cell viablilty of U937 cells in a dose-dependent manner, which was associated with induction of apoptotic cell death. Drynariae Rhizoma extracts treatment also reduced the levels of Bcl-xL anti-apoptotic protein expression and increased the levels of caspase-3, p53, pro-apoptotic protein, in U937 cells. RT-PCR data revealed that the level of bcl-2, bcl-xL mRNA expressions decreased in a dose-dependent manner. These findings suggest that Drynariae Rhizoma extracts may have induction of apoptotic cell death via regulation of several growth regulatory gene products. The abbreviations used are: FBS, fetal bovine serum; PBS, phosphate buffered saline; PI, propidium iodide; OD, optical density; DiOC6, 3,3-dihexyloxa carbcyanine iodide; MTT, 3 [4-5-dimethylthiazol-2-yl] -2-diphenyltetrazolium bromide.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1996년도 하계학술대회 논문집 C
• /
• pp.1787-1790
• /
• 1996

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권2호
• /
• pp.795-799
• /
• 2013

Ku70/80 heterodimer is a central element in the nonhomologous end joining (NHEJ) DNA repair pathway, Ku80 playing a key role in regulating the multiple functions of Ku proteins. It has been found that the Ku80 protein located at telomeres is a major contributor to radiosensitivity in some telomerase positive human cancer cells. However, in ALT human osteosarcoma cells, the precise function in radiosensitivity and telomere maintenance is still unknown. The aim of this study was to investigate the effects of Ku80 depletion in the U2OS ALT cell line cell line. Suppression of Ku80 expression was performed using a vector-based shRNA and stable Ku80 knockdown in cells was verified by Western blotting. U2OS cells treated with shRNA-Ku80 showed lower radiobiological parameters (D0, Dq and SF2) in clonogenic assays. Furthermore, shRNA-Ku80 vector transfected cells displayed shortening of the telomere length and showed less expression of TRF2 protein. These results demonstrated that down-regulation of Ku80 can sensitize ALT cells U2OS to radiation, and this radiosensitization is related to telomere length shortening.

• 수산해양기술연구
• /
• 제19권1호
• /
• pp.51-56
• /
• 1983

경계요소법에 의하여 U자형 홈(groove)을 가진 평판이 측면 분포하중을 받을 때 끝부에 위치할 원형 Hole의 최적 위치의 해석을 행하고 동일한 모델(model)에 대하여 실험결과를 비교하여 다음과 같은 결론을 얻게 되었다. 1. 원형 Hole이 U자형 홈(groove)에 가까이 접근하게 됨에 따라 초기의 원형 Hole은 c.-c.선에 수직방향이 그 장축이 되는 타원형으로 변화됨을 알 수 있다. 2. 원형 Hole이 U-groove에 접근함에 따라 Hole 과 Groove 사이의 Neck부는 그 횡단면응력이 인장 및 압축이 동시에 존재하다가 점점 전체가 인장역이 됨을 알 수 있다. 3. 원형 Hole이 Groove에 접근함에 따라 판재경계와 Hole 사이의 목(neck)부분은 계속적인 압축상태를 유지한다. 4. U-groove쪽의 Neck area의 응력분포는 그 최대응력치가 Hole로 접근함에 따라 가속적으로 증가하고 Hole의 찌그러짐(distortion)의 방지를 위한 Hole의 최적중심의 위치는 Neck부의 Hole 측면의 압축응력치가 0이 되지 않는 범위가 한계임을 알 수 있다.

• 대한두경부종양학회지
• /
• 제22권1호
• /
• pp.29-32
• /
• 2006

Background: The location of parotid gland tumors can influence the duration and the difficulty of the operation. If the information about tumor location was available preoperatively, it would allow accurate operative planning and counseling of patients in terms of the length of the operation and the potential morbidity. Methods: This study was based on a retrospective review of 100 patients with parotid gland tumors underwent parotidectomy from January 2000 to October 2005 at Yong-Dong Severance Hospital. Based on computed tomographic(CT) scan findings, 4 landmarks such as facial nerve(FN) line, Utrecht(U) line, Conn's are(CA), and retromandibular vein (RV) were drawn on the scans in same plane. The location of tumors were determined by the landmarks and confirmed by the operative findings. The accuracy of each landmarks was evaluated. To find out the accuracies according to tumor size, the tumors were divided into 2 groups; less than 2 cm and larger than 2 cm in diameter. Results: U line was the most accurate(94%), sensitive(89.3%) and specific(97.7%) in predicting tumor location of the parotid gland. However, in small tumors less than 2cm, FN line (p=0.022) and RV criteria (p=0.028) were more reliable in accuracy. Conclusion: CA, FN line, U line, and RV are all useful landmarks in preoperative prediction for the location of parotid gland tumors. However, U line was the most accurate, but we must consider that proper landmark should be used in prediction according to the size of tumor because the accuracy of landmark may change.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2005년도 추계종합학술대회
• /
• pp.179-184
• /
• 2005

Computer Education form is rapidly growing and has been developed in a variety of forms such as off-line. on-line, multimedia, ICT education . These forms are changed to E-Learning, Blended-Learning and Ubiquitous-Learning. Ubiquitous-Learning model schools have already been running on a national scale according to the policy of the Ministry of Education. In this study, to understand the necessity for the Ubiquitous-Learning and Ubiquitous-Environment, the awareness of the Ubiquitous environment and U-learning in model schools and general schools is analyzed and compared. Through this study, a desirable model about Ubiquitous-Learning and Environment will be explored.