• Title/Summary/Keyword: U-Box

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Optimization of Growth Medium Composition for Overproduction of Bacillus licheniformis Amylase in Recombinant Escherichia coli (Bacillus licheniformis amylase(BLMA)의 생산성 향상을 취한 재조합 대장균의 배지 최적화)

  • Nam, Seung-Hun;Lee, Woo-Jong;Byun, Tae-Gang;Seo, Jin-Ho;Park, Kwan-Hwa
    • Korean Journal of Food Science and Technology
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    • v.26 no.4
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    • pp.411-416
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    • 1994
  • The research is concerned with optimization of growth medium composition in an attempt to improve the product yield of Bacillus licheniformis amylase (BLMA) in recombinant E. coli containing the BLMA gene. BLMA has the catalytic activity of producing branched oligosaccharides from starch. The medium optimization was performed in flask cultures based on the Box and Wilson method. The optimized medium is composed of tryptone 18.0 g/l, yeast extract 22.4 g/l, NaCl 5.3 g/l and glucose 2.1 g/l. In a jar fermenter culture with the conventional LB medium, the recombinant E. coli yielded 1.39 g/l of final dry cell mass and 5.11 U/ml of enzyme activity. In the optimized medium, however, the final cell mass was increased to 6.01 g/l and the enzyme activity to 23.2 U/ml. Medium optimization improved cell mass by 4.3 times and enzyme activity by 4.5 times. Such an increase in enzyme activity is mainly due to an enhancement of cell mass.

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Experimental study on axial compressive behavior of welded built-up CFT stub columns made by cold-formed sections with different welding lines

  • Naghipour, Morteza;Yousofizinsaz, Ghazaleh;Shariati, Mahdi
    • Steel and Composite Structures
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    • v.34 no.3
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    • pp.347-359
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    • 2020
  • The objective of this study is to experimentally scrutinize the axial performance of built-up concrete filled steel tube (CFT) columns composed of steel plates. In this case, the main parameters cross section types, compressive strength of filled concrete, and the effect of welding lines. Welded built-up steel box columns are fabricated by connecting two pieces of cold-formed U-shaped or four pieces of L-shaped thin steel plates with continuous penetration groove welding line located at mid-depth of stub column section. Furthermore, traditional square steel box sections with no welding lines are investigated for the comparison of axial behavior between the generic and build-up cross sections. Accordingly, 20 stub columns with thickness and height of 2 and 300 mm have been manufactured. As a result, welding lines in built-up specimens act as stiffeners because have higher strength and thickness in comparison to the plates. Subsequently, by increasing the welding lines, the load bearing capacity of stub columns has been increased in comparison to the traditional series. Furthermore, for specimens with the same confinement steel tubes and concrete core, increment of B/t ratio has reduced the ductility and axial strength.

Implementation of MPEG-DASH based Low-Latency Live 360 VR Tiled Video Streaming Server (MPEG-DASH 기반 저지연 라이브 360 VR 분할영상 스트리밍 서버 구현)

  • Kim, Hyun Wook;Choi, U Sung;Yang, Sung Hyun
    • Journal of Broadcast Engineering
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    • v.23 no.4
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    • pp.549-558
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    • 2018
  • We designed and implemented streaming server based on MEPG DASH, which is able to provide high quality video with low-latency live streaming service like 360 VR video on the existing cable network via low-spec media service devices such as IPTV and OTT(Over the Top) SettopBox. We also designed and applied management process which is cable of supporting services by cashing streaming video file(MPD, Segment Files) to reduce the server response delay time. Further more, we confimred that it is also able to provide high quality of tiled video streaming with over 50,000kbps bitrate and 8K@60P through the experiment.

A Field Case on the Pilot Constructions and Changes of a Braced Cut Wall in a Coastal Filled Land (해안매립지반에서의 토류가시설 시험시공 및 변경사례)

  • Hwang, Young-Chul;Kim, Ki-Rim;Kim, Yeon-Jung
    • Proceedings of the Korean Geotechical Society Conference
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    • 2006.10a
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    • pp.46-55
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    • 2006
  • There are many kinds of braced cut wall methods as the sheet pile, SCW, CIP and slurry wall which is adoptable for a deep excavation construction in a coastal filled land. The braced cut wall which has a strong stiffness is very stable but it has the weak point that the construction cost is high. Thus when a braced cut wall is designed, the geotechnical engineers choose the braced cut wall which has more safe and economic in the consideration of surrounding buildings near the construction site. Especially, when the sheet pile method as a braced cut wall is cheesed, the layer order and consistence of a coastal deposit stratum are considered and the pile driving method is also considered. This paper introduces the case that the originally box-type sheet pile wall was changed to U-type and high strength material after the pilot test at the subway construction site in a coastal filled land. This paper also introduces the case that the sheet pile's driving method was changed to special method in the section of the temporary coffer dam which had made when the present coastal filled land was formed.

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A study on differences in the humor embedded in stories between Korean and American feature-length animation (한·미 장편 애니메이션에서 스토리와 관련된 유머의 차이에 대한 고찰)

  • Kim, Yun-Jung
    • Cartoon and Animation Studies
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    • s.35
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    • pp.67-88
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    • 2014
  • Internationally, the Korean feature-length animation is not making a successful box office hit. Being a box office hit all around the world is related with entertainment and laughter cannot be excluded as a element that gives such enjoyment. Although the humor would be playing an important role in globalization of Korean animation, studies that analyzed humor in Korean and U.S. animation are still lacking. Recently, there are cases that several Korean animations aim at overseas market entrust pre-production of scenario or storyboard. This paper examines the humor embedded in stories of feature-length animations that had the most differences by analyzing frequency and contents, and through interviews. It also proposes ways to improve the humor embedded in stories of Korean animations.

Influence of granite waste aggregate on properties of binary blend self-compacting concrete

  • Jain, Abhishek;Gupta, Rajesh;Chaudhary, Sandeep
    • Advances in concrete construction
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    • v.10 no.2
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    • pp.127-140
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    • 2020
  • This study explores the feasibility of granite waste aggregate (GWA) as a partial replacement of natural fine aggregate (NFA) in binary blend self-compacting concrete (SCC) prepared with fly ash. Total of nine SCC mixtures were prepared wherein one was Ordinary Portland cement (OPC) based control SCC mixture and remaining were fly ash based binary blend SCC mixtures which included the various percentages of GWA. Fresh properties tests such as slump flow, T500, V-funnel, J-ring, L-box, U-box, segregation resistance, bleeding, fresh density, and loss of slump flow (with time) were conducted. Compressive strength and percentage of permeable voids were evaluated in the hardened state. All the SCC mixtures exhibited sufficient flowability, passing ability, and resistance to segregation. Besides, all the binary blend SCC mixtures exhibited lower fresh density and bleeding, and better residual slump (up to 50% of GWA) compared to the OPC based control SCC mixture. Binary blend SCC mixture incorporating up to 40% GWA provided higher compressive strength than binary blend control SCC mixture. The findings of this study encourage the utilization of GWA in the development of binary blend SCC mixtures with satisfactory workability characteristics as a replacement of NFA.

A Study on the factors of Quality variation for High Flowing Concrete in Site (고유동 콘크리트의 품질변동 요인에 관한 연구)

  • Kwon Yeong-Ho;Lee Hyun-Ho;Lee Hwa-Jin;Ha Jae-Dam
    • Proceedings of the Korea Concrete Institute Conference
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    • 2004.11a
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    • pp.743-746
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    • 2004
  • This research investigates experimentally an effect on the properties of the high flowing concrete according to variations of concrete materials and site conditions. Variations of sensitivity test are selected items as followings; (1)Concrete temperature, (2)Unit water(Surface moisture of fine aggregate), (3)Fineness modulus of fine aggregate, (4)Addition ratio of high-range water reducing agent. And fresh conditions of the high flowing concrete should be satisfied with required range including slump flow$(65{\pm}5cm)$, 50cm reaching time of slump flow$(4\~10sec)$, V-box flowing time$(10\~20sec)$, U-box height(min.300mm) and air content$(4{\pm}1\%)$. As results of sensitivity test, material variations and site conditions should be satisfied with the range as followings; (1)Concrete temperature is $10\~20^{\circ}C$ (below $30^{\circ}C$), (2)Surface moisture of fine aggregate is within ${\pm}0.6\%$, (3)Fineness modulus of fine aggregate is $2.6{\pm}0.2$ and (4)addition ratio of high range water reducing agent is within $1\%$ considered flow-ability, self-compaction and segregation resistance of the high flowing concrete.

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A Study on the Hangeul confusion Character Recognition Using Fractal Dimensions and Attactors (프랙탈 차원과 어트랙트를 이용한 한글 혼동 문자 인식에 관한 연구)

  • Son, Yeong-U
    • The Transactions of the Korea Information Processing Society
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    • v.6 no.7
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    • pp.1825-1831
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    • 1999
  • In this paper, to reduce misrecognized characters, we propose the new method that extract features from character to apply to the character recognition using features from character to apply to the character recognition using fractal dimensions and attractors. Firstly, to reduce the load of recognizer we classify the characters. For the classified character, we extract the features for Box-counting dimensions. Natural Measures, Information dimensions then recognize characters. With histogram, we generate attractors and calculate dimensions from attractors. Then we recognize characters with dimensions of characters and attractors. An experimental result that the overall recognition rates for the training data and testing data are 96.03% and 91.74% respectively. This result shows the effectiveness of proposed method.

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Fresh and hardened properties of rubberized concrete using fine rubber and silpozz

  • Padhi, S.;Panda, K.C.
    • Advances in concrete construction
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    • v.4 no.1
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    • pp.49-69
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    • 2016
  • This work investigates the mechanical properties of conventional concrete (CC) and self compacting concrete (SCC) using fine rubber and silpozz were accompanied by a comparative study between conventional rubberized concrete (CRC) and self compacting rubberized concrete (SCRC). Fine rubber (FR) from scrap tires has replaced the fine aggregate (FA) and Silpozz has been used as a replacement of cement at the proportions of 5, 10 and 15%. Silpozz as a partial replacement of cement in addition of superplasticiser (SP) increases the strength of concrete. Fresh concrete properties such as slump test, compaction factor test for CRC, whereas for SCRC slump flow, $T_{500}$, V-funnel, L-box, U-box, J-ring tests were conducted along with the hardened properties tests like compressive, split tensile and flexural strength test at 7, 28 and 90 days of curing. The durability and microstructural behavior for both CRC and SCRC were investigated. FR used in the present study is 4.75 mm passing with fineness modulus 4.74.M30 grade concrete is used with a mix proportion of 1:1.44:2.91 and w/c ratio as 0.43. The results indicate that as FR quantity increases, workability of both CRC and SCRC decreases. The results also show that the replacement of natural fine aggregate (NFA) with FR particles decreases the compressive strength with the increase of flexural strength observed upto 5% replacement of FR. Also replacement of cement with silpozz resulted enhancement of strength in SCRC.

Effect of metakaolin on the properties of conventional and self compacting concrete

  • Lenka, S.;Panda, K.C.
    • Advances in concrete construction
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    • v.5 no.1
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    • pp.31-48
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    • 2017
  • Supplementary cementitious materials (SCM) have turned out to be a vital portion of extraordinary strength and performance concrete. Metakaolin (MK) is one of SCM material is acquired by calcinations of kaolinite. Universally utilised as pozzolanic material in concrete to enhance mechanical and durability properties. This study investigates the fresh and hardened properties of conventional concrete (CC) and self compacting concrete (SCC) by partially replacing cement with MK in diverse percentages. In CC and SCC, partial replacement of cement with MK varies from 5-20%. Fresh concrete properties of CC are conducted by slump test and compaction factor tests and for SCC, slump flow, T500, J-Ring, L-Box, V-Funnel and U-Box tests. Hardened concrete characteristics are investigated by compressive, split tensile and flexural strengths at age of 7, 28 and 90 days of curing under water. Carbonation depth, water absorption and density of MK based CC and SCC was also computed. Fresh concrete test results indicated that increase in MK replacement increases workability of concrete in a constant w/b ratio. Also, outcomes reveal that concrete integrating MK had greater compressive, flexural and split tensile strengths. Optimum replacement level of MK for cement was 10%, which increased mechanical properties and robustness properties of concrete.