• 제목/요약/키워드: TypeScript

검색결과 70건 처리시간 0.029초

Synthesis and base pairing properties of DNA-RNA heteroduplex containing 5-hydroxyuridine

  • Cui, Song;Kim, Yong-Hoon;Jin, Cheng-Hao;Kim, Sang-Kook;Rhee, Man-hee;Kwon, Oh-Shin;Moon, Byung-Jo
    • BMB Reports
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    • 제42권6호
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    • pp.373-379
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    • 2009
  • 5-Hydroxyuridine (5-OHU) is a major lesion of uridine and cytosine produced in RNA by various chemical oxidants. To elucidate its biochemical and biophysical effects on RNA replication, the site-specifically modified oligoribonucleotides containing 5-OHU were synthesized with C5-hydroxy-5'-ODMTr-2'-TBDMS-uridine phosphoramidite using automated solid phase synthesis. The base-pairing properties of nucleotides opposite 5-OHU in 24 mer oligoribonulcleotides with dNTP were studied using three reverse transcriptases (Super-$Script^{TM}II$-, AMV-, MMLV-RT) in cDNA synthesis. Adenine as well as guanine was incorporated preferentially by all reverse transcriptases. In the UV-melting temperature experiment, the results from the relative stabilities of the base pairs were A : 5-OHU > G : 5-OHU > T : 5-OHU $\approx$ C : 5-OHU. Circular Dichroism (CD) studies showed that DNA-RNA containing 5- OHU heteroduplexes exhibit a similar conformation between the A-type RNA and B-type DNA. These results suggest that 5- OHU from oxidative damage was mainly influenced by adenine mismatch.

Construction of web-based nutrition education contents and searching engine for usage of healthy menu of children

  • Hong, Soon-Myung;Lee, Tae-Kyong;Chung, Hea-Jung;Park, Hye-Kyung;Lee, Eun-Ju;Nam, Hye-Seon;Jung, Soon-Im;Cho, Jee-Ye;Lee, Jin-Hee;Kim, Gon;Kim, Min-Chan
    • Nutrition Research and Practice
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    • 제2권2호
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    • pp.114-120
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    • 2008
  • A diet habit, which is developed in childhood, lasts for a life time. In this sense, nutrition education and early exposure to healthy menus in childhood is important. Children these days have easy access to the internet. Thus, a web-based nutrition education program for children is an effective tool for nutrition education of children. This site provides the material of the nutrition education for children with characters which are personified nutrients. The 151 menus are stored in the site together with video script of the cooking process. The menus are classified by the criteria based on age, menu type and the ethnic origin of the menu. The site provides a search function. There are three kinds of search conditions which are key words, menu type and "between" expression of nutrients such as calorie and other nutrients. The site is developed with the operating system Windows 2003 Server, the web server ZEUS 5, development language JSP, and database management system Oracle 10 g.

Macrophage Colony-Stimulating Factor와 Osteoclast Differentiation Factor로 분화 유도된 생쥐 파골세포에서 Vitamin D 및 수종의 싸이토카인 수용체의 발현 (Expression of receptors of Vitamin D and cytokines in osteoclasts differentiated by M-CSF and ODF)

  • 성수미;엄흥식;고성희;우경미;장범석
    • Journal of Periodontal and Implant Science
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    • 제32권4호
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    • pp.865-873
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    • 2002
  • The primary cause of tooth loss after 30 years of age is periodontal disease. Destruction of alveolar bone by periodontal disease is done by bone resorbing activity of osteoclasts. Understanding differentiation and activation mechanism of osteoclasts is essential for controling periodontal disease. The purpose of this study is to identify the possible effects of Vitamin D and cytokines affecting osteoclasts and its precursor cells. Four to six week-old mice were killed and humerus, radius, tibia and femur were removed aseptically and washed two times with Hank's solution containing penicillin-streptomycin and then soft tissue were removed. Bone marrow cells were collected by 22 gauge needle. Cells were cultured in Hank's solution containing 1 mg/ml type II collagenase, 0.05% trypsin, 41mM EDTA. Supernatant solution was removed 5 times after 15 minutes of digestion with above mentioned enzyme solution, and remained bone particles were maintained in alpha-MEM for 15 minutes and $4^{\circ}C$ temperature. Bone particles were agitated for 1 minute and supernatant solution containing osteoclast precursor cells were filtrated with cell stainer. These separated osteoclast precursor cells were dispensed with 100-mm culture dish by $1{\times}10^7$ cells unit and cultured in ${\alpha}$- MEM containing 20 ng/ml recombinant human M-CSF, 30 ng/ml recombinant human soluble osteoclast differentiation factor and 10% fetal calf serum for 2 and 7 days. Total RNA of osteoclast precursor cells were extracted using RNeasy kit. One ${\mu}g$ of total RNA was reverse transcribed in $42^{\circ}C$ for 30 minutes using SuperScriptII reverse transcriptase. Expression of transcribed receptors of each hormone and cytokine were traced with 1 ${\mu}l$ of cDNA solution by PCR amplification. Vitamin D receptor WAS found in cells cultured for 7 days. TNF-${\alpha}$ receptor was found in cells cultured for 2 days and amount of receptors were increased by 7 days. IL-1 type I receptor was not found in cells cultured 2 and 7 days. But, IL-1 receptor type II was found in cells cultured for 2 days. TGF-${\alpha},{\beta}$type I receptor was found in cells cultured 2 and 7 days, and amount of receptors were increased by 7 days of culture. These results implies Vitamin D and cytokines can affect osteoclasts directly, and affecting period in differentiation cycle of osteoclasts is different by Vitamin D and cytokines.

페이로드 임베딩 사전학습 기반의 웹 공격 분류 모델 (Web Attack Classification Model Based on Payload Embedding Pre-Training)

  • 김연수;고영훈;엄익채;김경백
    • 정보보호학회논문지
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    • 제30권4호
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    • pp.669-677
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    • 2020
  • 인터넷 사용자가 폭발적으로 늘어나면서 웹을 이용한 공격이 증가했다. 뿐만 아니라 기존의 방어 기법들을 우회하기 위해 공격 패턴이 다양해졌다. 전통적인 웹 방화벽은 알져지지 않은 패턴의 공격을 탐지하기 어렵다. 따라서 인공지능으로 비정상을 탐지하는 방식이 대안으로 연구되고 있다. 특히 공격에 악용되는 스크립트나 쿼리가 텍스트로 이루어져 있다는 이유로 자연어 처리 기법을 적용하는 시도가 일어나고 있다. 하지만 스크립트나 쿼리는 미등록 단어(Unknown word)가 다량 발생하기 때문에 자연어 처리와는 다른 방식의 접근이 필요하다. 본 논문에서는 BPE(Byte Pair Encoding)기법으로 웹 공격 페이로드에 자주 사용되는 토큰 집합을 추출하여 임베딩 벡터를 학습시키고, 주의 메커니즘 기반의 Bi-GRU 신경망으로 토큰의 순서와 중요도를 학습하여 웹 공격을 분류하는 모델을 제안한다. 주요 웹 공격인 SQL 삽입 공격, 크로스 사이트 스크립팅, 명령 삽입 공격에 대하여 분류 평가 결과 약 0.9990의 정확도를 얻었으며, 기존 연구에서 제안한 모델의 성능을 상회하는 결과를 도출하였다.

Estimating USLE Soil Erosion through GIS-based Decision Support System

  • Her, Y.G.;Kang, M.S.;Park, S.W.
    • 한국농공학회논문집
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    • 제48권7호
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    • pp.3-14
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    • 2006
  • The objective of this study was to develop a GIS-based decision support system (GIS-USLE system) to estimate soil erosion and evaluate its effect on concentrated upland plots in Godang district, Korea. This system was developed for the ArcView environment using A VENUE script. Three modules were used in the GIS-USLE system, namely pre-processing, the USLE factors calculator module, and post-processing. This system benefits from a user friendly environment that allows users with limited computer knowledge to use it. This system was applied to 1,285 individual upland plots ranging from 0.005 to 1.347 ha in size with an average slope steepness of 14 %. The rainfall distributions were estimated using the three methods, namely Mononobe and Yen-Chow with Triangle and with Trapezoid type, and then used to calculate the rainfall erosivity factor. The soil erosion amounts from the 1,285 individual plots in the study area by 2 year return period with a 24h maximum rainfall amount of 154.6 mm were estimated at 5 tons/ha on average. Slope appeared to be the most important factor affecting soil erosion estimation, as expected. The prototype model was applied to the project area, and the results appeared to support the practical applications. By examining many fields simultaneously, this system can easily provide fast estimation of soil erosion and thus reveal the spatial pattern of erosion from fields in a region. This study will help estimate and evaluate soil erosion in concentrated upland districts and identify the best management practices.

디지털 타이포그래피에 관한 연구 (A Study of Digital Typography)

  • 홍석일
    • 디자인학연구
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    • 제21권
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    • pp.149-158
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    • 1997
  • 1984년 매킨토시 컴퓨터의 등장이래 나타난 몇 가지 중요한 기술적 성과는 컴퓨터 그래픽스 분야 뿐만 아니라 그래픽 디자인 프로세스와 그 미적 가치의 판단에도 큰 영향을 미쳐왔다. 우리 나라에서도 매킨토시 컴퓨터가 도입된 이래로 컴퓨터 서체의 개발이라든가 컴퓨터 디자인 프로세스 의한 출판업무가 활발하게 도입되었고, 그 영역이 확장되어 왔다. 현재 컴퓨터를 이용하여 서체를 전문적으로 개발하는 서체 디자인 회사도 많이 생겼고, 한글 서체 디자인만도 500여가지가 넘지만, 아직 컴퓨터에서 한글을 완벽하게 구현하는데 있어서는 여러 기술적인 제약뿐만 아니라 미적인 부분에 있어서도 몇 가지 문제점을 안고 있다. 따라서 지금까지의 컴퓨터를 이용한 서체디자인뿐만 아니라 전반적인 디지털 타이포그래피 분야를 살펴보고 새로운 가능성과 방향을 모색해 보고자 한다. 본 연구는 컴퓨터가 디자인분야에 도입된 이래 중요한 변화를 보여온 타이포그래피에 있어 컴퓨터의 역할과 그 기능을 고찰하고, 컴퓨터를 통하여 표현되는 타이포그래피의 조형적인 성격들을 전통적인 타이포그래피와 비교, 분석함으로써 컴퓨터의 기술적인 표현이 가져다주는 새로운 조형적 특성을 고찰하는데 그 목적이 있다.

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방송 구성작가의 업무 정체성과 노동경험: 구성작가들의 체험이 반영된 자기기술지 분석을 중심으로 (The Work Identity and Labor Experience of the Broadcasting Scriptwriters : Focusing on the Auto-ethnography that Reflects the Experiences of the Scriptwriters)

  • 김미숙
    • 한국콘텐츠학회논문지
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    • 제21권12호
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    • pp.645-661
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    • 2021
  • 구성작가가 우리나라 방송 제작시스템에 등장하여 핵심적인 생산 주체가 된 지 40년이 넘었다. 이 연구에서는 비드라마인 교양·예능·뉴스·라디오 등 다양한 방송 장르에서 프로그램을 기획·구성하고 자료조사와 섭외, 대본 집필에 이르기까지 무수한 역할을 해온 구성작가의 업무 정체성과 노동 경험에 구체적으로 알아보았다. 현업에서 일하는 20명의 구성작가의 자기기술지를 바탕으로 구성작가의 업무 정체성과 노동 경험에 대해 알아본 결과, 구성작가들은 '없어서는 안 될' 프로그램 생산 주체이자 미디어 문화생산자로서의 정체성을 가지고 있었으며 동시에 명확하지 않은 업무 분담으로 PD가 해야 할 일들을 떠맡고 있다고 느끼고 있었다. 이러한 불평등의 원인은 제작시스템과 고용형태의 문제라고 느끼고 있었으나 개별적으로 해결할 수 없다는 것을 인식하고 자신의 능력을 키워나가거나 일을 얻기 위한 인맥을 쌓고, 무조건 최선을 다하는 태도와 자신의 영역을 확장시키는 방법으로 각자도생의 생존전략을 펼치는 것으로 나타났다.

한글 단어 재인에 영향을 미치는 변인: 음절 형태를 중심으로 (Variables affecting Korean word recognition: focusing on syllable shape)

  • 민수영;이창환
    • 인지과학
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    • 제29권4호
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    • pp.193-220
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    • 2018
  • 시각 단어 재인에 영향을 미치는 변인으로는 단어 빈도, 단어 길이, 이웃단어, 단어 형태 등이 밝혀진 바 있다. 단어 형태 변인은 한글이 영어와는 글자 체계가 다르기 때문에 형태 정보가 단어 처리에 다른 방식으로 영향을 미칠 수 있다. 본 연구는 게스탈트의 연속성 원리를 한글에 적용하여 한글 단어의 처리 단위를 알아보고, 음절 형태가 한글 단어 재인에 영향을 미치는지 검증하고자 하였다. 실험 1에서는 3음절 단어를 사용하여 음절 유형(평소 읽기방향이 연속성 원리에 부합하는 '가로집자'형(예: "가"), 부합하지 않는 '세로집자'형(예: "고")과 글자 제시 방향(가로, 세로)을 조작하였다. 어휘 판단 시간의 분석 결과, 제시 방향에 상관없이 '가로집자'형의 처리 속도가 '세로집자'형보다 빨랐다. 실험 2에서는 차폐 점화를 사용하여 음절유형('가로집자'와 '세로집자'형) 및 점화 자극과 목표 자극의 시각적 관계(동일, 유사, 상이)를 조작하였다. 점화자극과 목표자극의 음절집자 형태에 따라서 수행에 차이가 있었으며 이는 음절 형태가 단어 재인에 미친다는 것을 시사한다.

중학교용 에너지 교육 자료 개발 연구 (A Study on Development of Energy Education Materials for Middle School Students)

  • 최돈형;이양락
    • 한국환경교육학회지:환경교육
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    • 제7권1호
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    • pp.46-87
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    • 1994
  • Our country has been consuming a huge amount of energy in the course of industrialization and its demand is expected to increase enormously in the future. However, the deposits of energy resources are so limited that the settlement of energy problem comes up the essential subject. To solve the energy problem, it is requested that new resources to gain energy stably should be developed and also energy should be economized and used effectively. The effective use of energy and an the wisdom of economy in energy are requested to everybody and these things should be habitualized from very young age through education. Nevertheless, almost every school in our country hasn’t been concerned about energy education. Even though they have a concern, they are very short of the energy education materials and the quality of the materials is not so good. Therefore it is very meaningful to the settlement of energy problem of the country to make the students who will lead our country to make the students who will lead our country in the future realize the seriousness of energy problem and to provide them the necessary knowledge and methods to solve this problem so that they practice those things in everyday life. Having these necessities, this research, supported by The Korea Energy Management Corporation(KEMCO), was performed for 8 months from April 17, 1994 to December 17, 1994. Many peoples participated in this study such as 30 staffs of researchers and authors, 5 staffs of photographers and illustrators, and 3 VCR program producers developing an energy education material set for middle school students that includes a printed material for student, a diskette for computer simulation, a teacher's guidebook, VCR material and its guidebook. The following main development direction was established : First, the material for student should be consisted of units that let students know the seriousness of energy problem. Second, the focus should be put on the necessary method and practice to economize energy actually in real life based on the basic knowledge learned in elementary school. Third, material for student should be consisted of modules to be student activity-oriented teaching-learning rather than lecture-oriented one. The activity, to maximize student's interests, should be presented in various forms such as experiments, investigation, play, data interpretation, computer simulation, visits, expression and appreciation, etc. To develop the energy education materials for middle school students, a research plan was made first. After literature review about domestic and foreign energy education materials, several research trips home and abroad, and discussion meetings, the basic theory of energy education such as the principle, objective, contents, teaching-learning method, and evaluation method was established. Material for student was developed through the following procedures : The activities in the existing energy education materials were analysed and were divided into four categories related to energy using places of home, school, community, and country, and which were again divided into three categories related to time of past, present, and future, Considering these division, nine modules which are structure units of material for student were chosen, Each module comprises 2-4 activities. Totally 31 activities were designed in this way. The syllabi were made out for each activity and writing was asked for to experts related to each activity after several discussions and revision. To complement the draft, another several discussions and revision were also made on it and then pictures and illustrations were asked for. All these procedures complete the material for student, titled ; Energy Inquiry of Middle School Students', which totals 129 pages and is all in color. As the manuscript of material for student was fixed, writing for teacher's guidebook was asked for to the same writers. The draft of teacher's guidebook was also complemented through the several concentrated works and discussions. Teacher's guidebook focused on the teaching-learning principle and methods of energy education and on the concrete instruction cases for effective instruction of material for student. It is organized with two parts : the one is 'general outline' which introduces theoretical contents and the other is 'details' which are practically helpful to teaching-learning. It is totally 131 pages including both 'general outline' and 'details'. The VCR material and its guidebook consist of contents that cultivate the good attitude trying to economize energy and raise student's interests with a purpose of strong motivation to recognize the necessity of economy and practice it. After establishing development direction of VCR material through discussion meetings and research trips, its script was made by relevant experts. Then the script was also reviewed two times. The drafted VCR material made by a video material developing expert was examined and modified by previews twice. After completion of VCR material, the VCR guidebook was made. All these procedures led to the development of VCR material which runs 20 minutes in VHS type. The VCR guidebook shows a production purpose of the program, structure of contents, evaluation methods, and contents of the program in detail to give help to instructors when they use this VCR material, When these energy education materials are used, it is desirable that the VCR material should be presented first to induce student's motive, and then material for student is introduced Since the material for student is composed of activity-oriented modules and each module is independent one another in general, and each activity is, too. the necessary module or activity can be chosen and utilized in any order according to school or class conditions. This energy education materials will contribute to the development of student's ability to solve energy problem in everyday life and teacher's ability to teach the fundamental knowledge and method in solving energy problem.

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네트노그라피를 이용한 공개 소프트웨어의 개발 및 확산 패턴 분석에 관한 연구 - 자바스크립트 프레임워크 사례를 중심으로 - (Tracing the Development and Spread Patterns of OSS using the Method of Netnography - The Case of JavaScript Frameworks -)

  • 강희숙;윤인환;이희상
    • 경영과정보연구
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    • 제36권3호
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    • pp.131-150
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    • 2017
  • 본 연구의 목적은 공개 소프트웨어(Open Source Software, 이하 OSS)가 운영 기간 내 주변의 행위자들과 관계를 수립하는 동안 OSS의 개발 및 확산 패턴을 확인하는 것으로, OSS 참여자들의 변화 패턴을 조사하기 위해 OSS 통과시간을 기반으로 그 변화 양상을 추적할 수 있는 온라인 데이터와 네트노그라피 방법을 이용하였다. 이를 위해 대표적인 OSS 자바스크립트 프레임워크인 jQuery, MooTools, YUI 등 이상 세 가지 사례에 대하여 블로그, 웹 서치와 함께 GitHub 공개 API(Application Programming Interface)로 수집된 데이터를 활용하였다. 본 연구에서는 OSS 변형 과정의 변화 패턴을 분류하기 위하여 행위자-네트워크 이론의 전환(translation) 과정을 적용하였으며, 관찰된 OSS 변형 과정을 살펴보면 다음과 같다. 먼저, '프로젝트 개시' 단계에서 소스 코드, 프로젝트 책임자 및 관계자, 내부 참여자 등과 같은 세 가지 유형의 OSS 관련 행위자들을 확인하였고, 그들 사이의 관계성을 개념화 하였다. 이후 프로젝트 책임자가 최초로 프로젝트를 착수하는 '프로젝트 성장' 단계는 관계자들에 의해 소스 코드가 유지 보수되는 과정을 통해 개선된다. 마지막으로 OSS는 홍보 활동을 통해 참여자들의 관찰기를 갖고, 소스 코드 사용을 통해 학습기를 거친 사용자가 본격적으로 등장함으로써 '참여자의 도약' 단계로 진입한다. 이 시기에는 기업과 외부 관계자들도 출현하는 모습도 살펴볼 수 있다. 본 연구결과는 OSS 참여자들이 OSS를 선택하는데 있어 홍보 과정의 중요성을 강조하고, OSS의 급속한 개발속도가 오히려 참여자의 출현을 지연시키는 구축 효과(crowding-out effec)가 발생하는 것을 확인하였다. 본 연구는 행위자-네트워크 이론을 토대로 주요 OSS 사례를 네트노그라피를 활용하여 종단적인 관점에서 분석함으로써 OSS의 발전 과정을 일반화시키기 위한 노력을 시도했다는 점에서 학술적인 의의가 있으며, OSS가 지배적인 위치에 오르기 위한 단계별 영향 요인, 세부적인 변화 양상 등을 확인함으로써 OSS 개발자와 관리자들에게 다양한 시사점을 제공할 것으로 기대된다.

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