• BMB Reports
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• v.33 no.6
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• pp.519-524
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• 2000

NF1 proteins are a family of DNA binding proteins which consist of two separate domains, N-terminal DNA binding domain and C-terminal transcription activation domain. The N-terminal 220 amino acids are highly conserved and are also known to mediate dimerization of NF1 proteins. The C-terminal regions of different type of NF1 proteins are heterogeneous and responsible for transcriptional activation. In this study, we tested the interaction between different domains of rat NF1-A protein by yeast two hybrid analysis and observed the interaction between C-terminal regions of NF1-A which do not contain the N-terminal dimerization domain. Our results showed that the C-terminal region of rat NF1-A between residues 231 and 509 strongly interacted not only with itself, but also with human NF1/CTF1 which is a different type of NF1. When the C-terminal region was divided into two fragments, one from residue 231 to 447 and the other from 448 to 509, the two fragments were able to interact with the C-terminal region of NF1-A significantly. This indicates that both fragments contain independent interaction domains. Analysis of the interactions with alanine substituted fragments showed that substitutions of the heptasequence, SPTSPTY of NF1-A, affected interaction between NF1 proteins. Our results strongly suggest that C-terminal regions may also be important for the formation of homo- and heterodimers in addition to the N-terminal dimerization domain. Also, the heptasequence motif may play some roles in dimer formation.

• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1303-1307
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• 2007

A tumor suppressor, merlin is a member of ERM family proteins. It consists of N-terminal FERM domain, ${\alpha}-helical$ region, and C-terminal domain. Alternative splicing of merlin's mRNA generates two isotypes of merlin. Isotype I, which has exon17 at the C-terminus instead of exon16 in isotype II, is known to have tumor suppressor activity. Like other ERM proteins, the C-terminal domain of merlin isotype I interacts to its FERM domain. That of isotype II, however, was reported not to bind FERM domain despite the large common part of C-terminal domain, which possibly binds FERM domain. Here, we show the binding of FERM domain to both C-terminal domains of merlin's two isotypes by isothermal titration calorimetry. These results support that merlin isotype II also can form a closed conformation or a multimer by intramolecular or intermolecular interactions using their FERM domain and C-terminal domain.

• Nuclear Engineering and Technology
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• v.46 no.5
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• pp.681-688
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• 2014

Hydride reorientation behaviors of PWR cladding tubes under typical interim dry storage conditions were investigated with the use of as-received 250 and 485ppm hydrogen-charged Zr-Nb alloy cladding tubes. In order to evaluate the effect of typical cool-down processes on the radial hydride precipitation, two terminal heat-up temperatures of 300 and $400^{\circ}C$, as well as two terminal cool-down temperatures of 200 and $300^{\circ}C$, were considered. In addition, two cooling rates of 2.5 and $8.0^{\circ}C/min$ during the cool-down processes were taken into account along with zero stress or a tensile hoop stress of 150MPa. It was found that the 250ppm hydrogen-charged specimen experiencing the higher terminal heat-up temperature and the lower terminal cool-down temperature generated the highest number of radial hydrides during the cool-down process under 150MPa hoop tensile stress, which may be explained by terminal solid hydrogen solubilities for precipitation, and dissolution and remaining circumferential hydrides at the terminal heat-up temperatures. In addition, the slower cool-down rate generates the larger number of radial hydrides due to a cooling rate-dependent, longer residence time at a relatively high temperature that can accelerate the radial hydride nucleation and growth.

• Geomechanics and Engineering
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• v.23 no.5
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• pp.493-502
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• 2020

The prediction of soil response under repetitive mechanical loadings remains challenging in geotechnical engineering applications. Modeling the cyclic soil response requires a robust model validation with an experimental dataset. This study proposes a unique method adopting linearity of model constant with the number of cycles. The model allows the prediction of the terminal density of sediments when subjected to repetitive changes in pore-fluid pressure based on the two-surface plasticity. Model simulations are analyzed in combination with an experimental dataset of sandy sediments when subjected to repetitive changes in pore fluid pressure under constant deviatoric stress conditions. The results show that the modified plastic moduli in the two-surface plasticity model appear to be critical for determining the terminal density. The methodology introduced in this study is expected to contribute to the prediction of the terminal density and the evolution of shear strain at given repetitive loading conditions.

• The Journal of the Korea Contents Association
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• v.13 no.5
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• pp.408-419
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• 2013

The terminals of e-Book(electronic Book) are classified into two types which consist of special-purpose terminal equipment and general-purpose terminal equipment. Although the terminal products inr the two types have had a strong race in the e-Book market each other, both enterprises and consumers want the two type to remain persistently with competition in good faith. Therefore, it is necessary for each type camps to find blue ocean by establishing differentiation strategies of each type. In this paper, we designed the research model that independent variables consist of hardware quality and software quality, dependent variable is user satisfaction, moderator variable is the terminal type. Through the research model, we investigated factors having a influence on the user satisfaction of the e-Book terminal that consists of hardware and software. In particular, we investigated if the degree of the influence is different between the two types. As a result, we got conclusions as followings. First, the quality of hardware and software in the terminal had significant influences on the user satisfaction. Second, the degree which the hardware quality has influenced user satisfaction was not different between the two types, but the degree which the software quality has influence on user satisfaction was different between the two types. As a implication of this result, we found that special-purpose terminal provider need to establish a strategies for raising the quality of e-Book viewer to provide different product from general-purpose terminal.

• Biomedical Science Letters
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• v.9 no.2
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• pp.105-110
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• 2003

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. Canonical ankyrins are 190-220 kDa proteins expressed in most tissues and cell types and comprise a membrane-binding domain (MBD) of 24 ANK repeats, a spectrin-binding domain, a death domain and a C-terminal domain. Rescue studies with ankyrin-B/G chimeras have identified the C-terminal domain of ankyrin-B as the defining domain in specifying ankyrin-B activity, but the function of C-terminal domain of ankyrin-B is, however, not known. We report here that the C-terminal domain of ankyrin-B is capable of interacting with the C-terminal Region of Hsp40. The Hsps are induced not only by heat shock but also by various other environmental stresses. Hsps are also expressed constitutively at normal growth temperatures and have basic and indispensable functions in the life cycle of proteins as molecular chaperones, as well as playing a role in protecting cells from the deleterious stresses. The binding sites required in the interaction between C-terminal domain of ankyrin-B and C-terminal region of Hsp40 were characterized using the yeast two-hybrid system and GST-pull down assay. The interaction between ankyrin-B and Hsp40 represents the first direct evidence of ankyrin's role as chaperones.

• BMB Reports
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• v.34 no.1
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• pp.15-20
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• 2001

One of the innate immune reactions in invertebrates is the pro-phenoloxidase (pro-PO) activation system that is involved in the generation of superoxide, melanin synthesis, and the subsequent sequestration of foreign matter entering the hemocoel of the invertebrates. However, the molecular mechanism of this biological reaction is still obscure. To expand our understanding of the biological roles of the pro-PO activation system in invertebrates, we performed a yeast two-hybrid screening by using three regions of pro-PO as bait and a yeast two-hybrid cDNA library from Tenebrio molitor larvae as prey We isolated a novel partial cDNA clone that encodes a glycine-rich protein that interacted with the active phenoloxidase (termed phenoloxidase interacting protein, POIP). POIP consists of two domains: One is an N-terminal unique domain and the other is a C-terminal glycine-rich domain. The C-terminal glycine-rich domain showed sequential homology with those of insect antifungal proteins. Also, the yeast two-hybrid screen in a reverse orientation (using POIP as bait) yielded PO, suggesting that the PO-POIP interaction is specific. By using a 315 bP PCR fragment of the N-terminal unique region of POIP, we cloned the full-length cDNA of POIP from the Tenebruo cDNA library constructed by using E. coli injected larvae. The interaction analysis between PO, and a truncated fragment lacking the N-terminal unique region of POIP, indicated that the N-terminal unique region is necessary for interaction between PO and POIP. The expression level of the POIP mRNA is increased by bacterial injection into T. molitor larvae. This suggests that POIP might be engaged in the humoral defense reaction.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1991.10a
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• pp.48-51
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• 1991

Short channel Nonvolatile EEPROM memory devices were fabricated to CMOS 1M bit design rule, and reviews the characteristics and applications of SNOSFET. Application of SNOS field effect transistors have been proposed for both logic circuits and nonvolatile memory arrays, and operating characteristics with write and erase were investigated. As a results, memory window size of four terminal devices and two terminal devices was established low conductance stage and high conductance state, which was operated in “1” state and “0”state with write and erase respectively. And the operating characteristics of unit cell in matrix array were investigated with implementing the composition method of four and two terminal nonvolatile memory cells. It was shown that four terminal 2${\times}$2 matrix array was operated bipolar, and two termineal 2${\times}$2 matrix array was operated unipolar.

• Journal of the Korean Operations Research and Management Science Society
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• v.14 no.1
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• pp.88-96
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• 1989

General measure in the reliability is the k-terminal reliability, which is the probability that the specified vertices are connected by the working edges. To compute the k-terminal reliability components are usually assumed to be statistically independent. In this study the modeling and analysis of the k-terminal reliability are investigated when dependency among components is considered. As the size of the network increases, the number of the joint probability parameter to represent the dependency among components is increasing exponentially. To avoid such a difficulty the structured-event-based-reliability model (SERM) is presented. This model uses the combination of the network topology (physical representation) and reliability block diagram (logical representation). This enables us to represent the dependency among components in a network form. Computational algorithms for the k-terminal reliability in SERM are based on the factoring algorithm Two features of the ractoring algorithm are the reliability preserving reduction and the privoting edge selection strategy. The pivoting edge selction strategy is modified by two different ways to tackle the replicated edges occuring in SERM. Two algorithms are presented according to each modified pivoting strategy and illustrated by numerical example.

• Journal of the Korean Home Economics Association
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• v.41 no.7
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• pp.25-38
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• 2003

This study explored the terminal values(Rokeach's Value) and 5-money spending styles of girls in Yanbian, China and those in South Korea. The subjects were 466 school girls in Yanbian, China and 498 high school girls in South Korea. We used Cronbach' α, t-test, and Pearson correlation analysis as statistical analysis. The results were summarized as follows : 1) at the top of the terminal value hierarchy of girls in the two countries were family security and happiness. 2) resulting from the t-test, there found significant differences between the 5-money spending styles of the Yanbian girls and that of the Korean girls. 3) resoling from the Pearson correlation analysis, there found similarities and differences in relationship between terminal values and money spending styles in two groups.