• Bulletin of the Korean Chemical Society
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• 제31권3호
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• pp.599-605
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• 2010

A two-photon fluorescent probe (ACaCL) that can detect near-membrane $Ca^{2+}$ is reported. This probe can be excited by 780 nm fs pulses, shows high photostability and negligible toxicity, and can visualize near-membrane $Ca^{2+}$ in live cells and deep inside live tissues by two-photon microscopy.

• Bulletin of the Korean Chemical Society
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• 제32권spc8호
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• pp.2889-2890
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• 2011

• Bulletin of the Korean Chemical Society
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• 제28권12호
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• pp.2253-2260
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• 2007

A series of tetra donor substituted [2.2]paracyclophane-based two-photon absorption (TPA) fluorophores were synthesized in neutral and cationic forms. The imaging activity of overall set of fluorophores was studied by the two-photon induced fluorescence (TPIF) method in a range of solvents. We also measured a clear progression toward a longer photoluminescence lifetime with increasing solvent polarity (intrinsic photoluminescence lifetime, τi: ~2 ns in toluene → 12-16 ns in water). The paracyclophane fluorophores with this unique property can be utilized as an optical polarity probe for the biomolecular substrates. The combined measurement of the two-photon fluorescence microscopy (TPM) cell image and TPIF lifetime can give us a better understanding of the biological processes and local environments in the cells.

• Journal of the Optical Society of Korea
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• 제1권2호
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• pp.100-103
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• 1997

We perform degenerate and nondegenerate pump-probe experiments on bulk GaAs at 100 K below the band gap. We mostly observe a negative differential transmission signal both in the degenerate and nondegenerate experiments. We interpret our signal as due to two-photon absorption. This negative signal has a different origin from the normally considered band gap renormalization for resonant excitations.

• 대한의용생체공학회:의공학회지
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• 제32권2호
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• pp.85-92
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• 2011

Bioluminescence imaging (BLI) is the most sensitive animal imaging technique for molecular imaging research. Generally, highly sensitive CCD is used to detect an optical probe introduced in a living mouse. However, in many cases, the light signal emitted from a probe is too small to detect because it is scattered and attenuated by the tissue prior to being detected. The problem is that scattering and attenuation not only inhibit accurate measurement but also make image quality down. Thus we introduced a new method to reduce noise by using property of CCD and method to improve image quality of bioluminescence image by using two steps Gaussian blurring.

• Journal of the Optical Society of Korea
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• 제14권4호
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• pp.431-437
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• 2010

A stack of gradient-index (GRIN) rod lenses cannot be used for coherent anti-Stokes Raman scattering (CARS) microendoscopy for insertion to internal organs through a surgical keyhole with minimal invasiveness. That's because GRIN lens has large amount of inherent chromatic aberrations in spite of absolutely requiring a common focus for pump and Stokes beam with each frequency of ${\omega}_p$ and ${\omega}_S$. For this endoscopic purpose, we need to develop a long slender probe-type objective, namely probe-type microscope objective (PMO). In this paper, we introduce the structure, the working principle, and the design techniques of PMO which is composed of a probe-type lens module (PLM) and an adaptor lens module (ALM). PLM is first designed for a long slender type and ALM is successively designed by using several design parameters from PLM for eliminating optical discords between scanning unit and PLM. A combined module is optimized again to eliminate some coupling disparities between PLM and ALM for the best PMO. As a result, we can obtain a long slender PMO with perfectly diffraction-limited performance for pump beam of 817 nm and Stokes beam of 1064 nm.

• Bulletin of the Korean Chemical Society
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• 제34권8호
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• pp.2241-2242
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• 2013

• 한국광학회지
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• 제17권4호
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• pp.305-311
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• 2006

우리는 포화흡수 분광에서 이광자 결맞음 효과를 고려한 7준위 원자 모델을 제안하고 이를 이용하여 Nakayama이론으로 설명할 수 없었던 레이저의 세기에 따른 스펙트럼의 변화와 일부 교차공진선에서 Nakayama이론과의 불일치 현상을 설명하였다. 우리는 Zeeman 부준위에서 펌프광과 조사광의 편광이 $\pi-\pi$일 때를 모두 고려하여 상준위 2개 하준위 5개로 구성된 7준위의 모델을 만들었고, $^{85}$Rb원자의 5S$_{1/2}$ - 5P$_{1/2}$ 전이선에 대하여 4준위 Nakayama이론과 7준위 원자 모델을 비교하였다. 또한 레이저의 세기에 따른 포화흡수 분광 스펙트럼과 7준위 원자모델로 계산한 이론결과가 잘 일치함을 보였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.179-179
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• 2012

Low pressure radio-frequency glow discharges are investigated using theoretical modeling and various experimental diagnostic methods. In the calculations, global models and transformer models are developed to understand the chemical kinetics as well as the electrical properties such as the effective collision frequency, the heating mechanism and the power transferred to the plasma electrons. In addition, Boltzmann equation solver is used to compensate the effect of the electron energy distribution function (EEDF) shape in the global model, and the general expression of energy balance for non-Maxwellian electrons is developed. In the experiments, a number of traditional plasma diagnostic methods are used to compare with calculated results such as Langmuir probe, optical emission spectroscopy (OES), optical absorption spectroscopy (OAS) and two-photon absorption laser-induced fluorescence (TALIF). These theoretical and experimental methods are applied to understand several interesting phenomena in low pressure ICP discharges. The chemical and physical properties of low pressure ICP discharges are described and the applications of these methods are discussed.