• Journal of the Korea Society for Simulation
• /
• v.17 no.4
• /
• pp.41-50
• /
• 2008

In this study, an improved scheme for distance-based registration (DBR) is proposed and its performance is analyzed. In the DBR, when a mobile station (MS) enters a new cell, it calculates the distance between last registered cell and current cell and registers its location if the distance reaches reference distance D. In this study, two-location DBR (TDBR) is proposed to improve the performance of the DBR. In the TDBR, an MS stores not only last registered location area (LA) but also previously registered LA, and then no registration is needed when the MS crosses two LAs stored already. However, since the TDBR may increase paging cost, trade-off is necessary between decreased registration cost and increased paging cost. In this study, the performances of two schemes are analyzed and compared using 2-dimensional random walk mobility model in hexagonal cell configuration. We show that our mathematical analysis is accurate by comparing with simulation. From the numerical results for various circumstances, it is shown that our proposed TDBR outperforms current DBR in most cases.

• Journal of Pharmacopuncture
• /
• v.14 no.1
• /
• pp.79-86
• /
• 2011

Objective : This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Cuscutae Semen(CS) extracted with two kinds of solvents, ethanol and distilled water. Methods : Two kinds of CS extractions were prepared 20, 50, 100 ${\mu}l/mg$. The cytotoxicity was measured by MTT assay in Raw 264.7 cell. The anti-inflammation effect was measured by inhibitory efficacy of NO Production in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes. Results : 1. Two kinds(100 ${\mu}l/mg$) of CS extraction groups had 50% cytotoxicity in Raw 264.7 cell. 2. All of CS extraction groups were not showed significantly inhibitory effect on NO production. 3. All of CS extracted with ethanol only showed dose-dependently significantly scavenging effect of DPPH radicals. 4. Two kinds of CS extractions did not have a inhibitory effect on Propionibactrium acnes. Conclusion : Two kinds(100 ${\mu}l/mg$) of CS extraction groups have 50% cytotoxicity. Two kinds of CS extractions have not the inhibitory effect on NO production and Propionibactrium acnes. CS groups extracted with ethanol only have a significantly scavenging ability of DPPH radicals. This study suggests that CS extracted with ethanol was effective in anti-oxidation.

• YAKHAK HOEJI
• /
• v.52 no.1
• /
• pp.48-55
• /
• 2008

CD29 $({\beta}1-integrins)$ is one of major adhesion molecules involved in regulating cell adhesion, migration and morphological changes. In this study, we investigated the regulatory role of CD29 in monocytic functions using monocytic cell line U937 cells. CD29 was found to be one of highly expressed membrane proteins in U937 cells, according to flow cytometric analysis. The activation of CD29 by agonistic antibody MEM101A and extracellular matrix protein (ECM) fibronectin strongly induced cell-cell and cell-fibronectin adhesions. However, blocking antibodies to CD98 and CD147 showed different inhibitory features in these two adhesion events. Furthermore, U0126, an ERK inhibitor, only blocked cell-cell adhesion but not cell-fibronectin adhesion, indicating that cell-cell or cell-fibronectin adhesion events may be regulated by different molecular mechanisms. Meanwhile, CD29 activation also enhanced ROS generation but not phagocytic ability, and similarly radical scavenger N-acetyl-L-cysteine strongly blocked CD29-mediated cell-cell adhesion, implying that ROS may play a critical role in up-regulating cell-cell adhesion. Therefore, our data suggest that the activation of CD29 may be critically involved in regulating monocytic cell-mediated cell-cell adhesion and ROS generation.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.9
• /
• pp.1620-1628
• /
• 2016

In this study, we investigated colistin resistance mechanisms associated with the regulation of the pbgP operon in Klebsiella pneumoniae, using four isogenic pairs of colistin-susceptible strains and their colistin-resistant derivatives and two colistin-resistant clinical isolates. Amino acid sequence alterations of PhoPQ, PmrAB, and MgrB were investigated, and mRNA expression levels of phoQ, pmrB, pmrD, and pbgP were measured using quantitative real-time PCR. The phoQ and pmrB genes were deleted from two colistin-resistant derivatives, 134R and 063R. We found that phoQ, pmrD, and pbgP were significantly upregulated in all colistin-resistant derivatives. However, pmrB was significantly upregulated in only two colistin-resistant derivatives and one clinical strain. pmrB was not overexpressed in the other strains. The minimum inhibitory concentration of colistin was drastically lower in both phoQ- and pmrB-deleted mutants from a colistin-resistant derivative (134R) that was overexpressing phoQ and pmrB. However, colistin susceptibility was restored only in a phoQ-deleted mutant from a colistin-resistant derivative (063R) without overexpression of pmrB. In conclusion, two different regulations of the pbgP operon may associate with the development of colistinresisant K. pneumoniae.

• Journal of Multimedia Information System
• /
• v.2 no.4
• /
• pp.303-310
• /
• 2015

Vulnerable road users such as bike, motorcycle, small automobiles, and etc. are easily attacked or threatened with bigger vehicles than them. So this paper suggests a new approach two-wheelers detection system riding on people based on modified histogram of oriented gradients (HOGs) which is weighted by curvature and local correlation coefficient. This correlation coefficient between two variables, in which one is the person riding a bike and other is its background, can represent correlation relation. First, we extract edge vectors using the curvature of Gaussian and Histogram of Oriented Gradients (HOG) which includes gradient information and differential magnitude as cell based. And then, the value, which is calculated by the correlation coefficient between the area of each cell and one of bike, can be used as the weighting factor in process for normalizing the HOG cell. This paper applied the Adaboost algorithm to make a strong classification from weak classification. The experimental results validate the effectiveness of our proposed algorithm show higher than that of the traditional method and under challenging, such as various two-wheeler postures, complex background, and even conclusion.

• Biomedical Science Letters
• /
• v.17 no.1
• /
• pp.69-77
• /
• 2011

In this study, we evaluated the protective effects of supercritical extracts and two step ethanol extracts after supercritical extraction from Schizandra chinensis on antioxidant activities and oxidative DNA and cell damages. Supercritical extracts removed DPPH (1,1-diphenyl-2-picryldrazyl) radical by 85.5% at 200 ${\mu}g$/ml, but showed low activities of scavenging and chelating the hydroxyl radical and ferrous iron. However, two step ethanol extracts showed low activities of scavenging the DPPH radical, but removed the hydroxyl radical by 86% at 200 ${\mu}g$/ml. In addition, we tested the activities of extracts for reducing hydroxyl radical-induced DNA and cell damage. Two step ethanol extracts showed protective effect against the oxidative DNA damage by reducing DNA segmentation, inhibiting DNA migration and decreasing the expression of phospho-H2AX. Also, two step ethanol extracts showed protective effect against the oxidative cell damage by inhibiting lipid peroxidation and increasing the expression of p21 protein. Taken together, we suggest that two step ethanol extracts from S. chinensis have a role as useful inhibitors against oxidative damages.

• Korean Parent-Child Health Journal
• /
• v.15 no.2
• /
• pp.60-70
• /
• 2012

Purpose: This study was done to examine factors influencing cell phone addiction for middle school students by gender. Methods: The participants were 228 male students and 228 female students in two middle schools. Data were collected through self-report questionnaires, and analyzed using the SPSS/WIN 19.0 program. Results: Cell phone addictions of female students are higher than those of male students. Factors influencing cell phone addiction for male students were mimicry, sending text message on weekdays, immediate self-control, grade, syntony, and monthly call charge, explaining 42.2% of variance in cell phone addiction. Factors influencing cell phone addiction for female students were internet addiction, sending and receiving text message on weekends, immediate self-control, long-term self-control, use time, main use, syntony, and monthly call charge, explaining 46.8% of variance in cell phone addiction. Conclusion: The results indicated that cell phone addiction and its influencing factors differed by gender. Therefore the approach to effective cell phone addiction management program for middle school students should consider gender differences.

• Journal of Korea Multimedia Society
• /
• v.17 no.10
• /
• pp.1150-1159
• /
• 2014

This paper proposes a method for extracting tissue cells from an organism image by an electron microscope and getting the whole cell number and the area from the cell. In general, the difference between the cell color and the background is used to extract tissue cell. However, there may be a problem when overlapped cells are seen as a single cell. To solve the problem, we split them by using cell size and curvature. This method has a 99% accuracy rate. To measure the cell area, we compute two areas, the inside and boundary of the cell. The inside is simply calculated by the number of pixels. The cell boundary is obtained by applying super sampling, linear interpolation, and cubic spline interpolation. It improves the error rate, 18%, 19%, and 120% respectively, in comparison to the counting method that counts a pixel area as 1.

• Journal of Embryo Transfer
• /
• v.11 no.3
• /
• pp.217-223
• /
• 1996

This study was conducted to investigate the effects of co-culture for the development rate to morula /blastocyst stages of early porcine embryos, derived from oocytes matured and fertilized in vitro, with porcine endometrial cell monolayers(PEM) in the two different media, respectively. The rates of embryos developed to 2-, 4-, 8~16-cell and morula /blastocyst stage were 49.6, 40.5, 28.2 and 15.3% in Ham's F-10 with PEM, and 55.3, 45.9, 32.7, and 17.6% in TCM-HEPES with PEM, respectively. The above development rates to morula /blastocyst stages were significantly higher than those of the embryos cultured in the Ham's F-10 and TGM-HEPES without PEM(P<0.05). The in vitro development rates to the morula /blastocyst stage of 1-cell embryos cultured in Ham's F-10 and TCM-HEPES without PEM were 0~1.2%. Especially, most of embryos were observed to arrest the development beyond 4-cell stages. As shown in the above results, the co-culture of in vitro produced porcine embryos with PEM in the two different media enhanced the development of fertilized eggs to morula /blastocyst stages in vitro. However, we didn't find out any differences for the in vitro development to morula /blastocyst stages between Ham's F-10 and TcM-HEPES media.

• Proceedings of the KIPE Conference
• /
• 2008.06a
• /
• pp.274-276
• /
• 2008

This paper proposes an individual charge equalization converter using selective two current paths for series connected lithium-ion battery strings. In the proposed equalizer, a central equalization converter acting as a controllable current source is sequentially connected in parallel with individual batteries through an array of cell selection switches. A flyback converter with a modified rectifier realizes a controllable current source. A central equalization converter is shared by every battery cells through the cell selection switch, instead of a dedicated charge equalizer for each cell. With this configuration, although the proposed equalizer has one dc-dc converter, individual charge equalization can be effectively achieved for the each cell in the strings. Furthermore, since the proposed equalizer would not allocate the separated dc-dc converter to each cell, such that the implementation of great size reduction and low cost can be allowed. In this paper, an optimal power rating design guide is also employed to obtain a minimal balancing size while satisfying equalization requirements. A prototype for eight lithium-ion battery cells is optimally designed and implemented. Experimental results verify that the proposed equalization method has good cell balancing performance showing small size, and low cost.