Kim, Myung-Suk;Sim, Sang-Soo;Yoon, Shin-Hee;Han, Sang-Jun;Kim, Chung-Chin;Choi, Hyun
The Korean Journal of Physiology
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v.21
no.2
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pp.259-272
/
1987
This was study carried out to investigate the effect of calcium on spontaneous contraction and electrical activity induced by ethanol in gastric smooth muscle. After peeling off the mucous membrane from the isolated whole stomach of 102 cats, two kinds of small muscle preparations $(2.0{\times}0.2\;cm)$, one longitudinal and the other circular, were excised from the fundus, the corpus and the antrum portion of each whole stomach specimen. The isometric contraction of the small muscle preparation was measured in a cylinder-shaped chamber filled with Krebs-Ringer-dextrose solution (pH 7.4, temperature $36{\pm}0.5^{\circ}C$) bubbling with 5% $CO_2$ in $O_2$. A large muscle preparation $(5.0{\times}1.2\;cm)$ was excised from the anterior wall of the corpus-antrum portion of the same specimen in 72 of 102 cats. The gastric electrical activity (slow wave and spike potential) was monopolarly recorded by four capillary electrodes (Ag-AgCl), of which two were placed on the corpus and two on the antrum, in a muscle chamber filled with the same solution as described above. Changes in the amplitude of the contraction, frequency of the gastric slow wave and the production of the spike potential were observed after adding ethanol and/or under the treatments with verapamil, $CaCl_2$ and Ca-free Krebs-Ringer-dextrose solution. The results were as follows: 1) After adding ethanol, the spontaneous phasic contraction of the corpus was reduced dose-dependently (0.125-2.0%), which was totally abolished by higher concentrations (2.0-8.0%) of ethanol. 2) The corporal phasic contraction was also completely abolished by verapamil $(3{\times}10^{-5}\;M)$ or Ca-free Krebs-Ringer-dextrose solution. The contraction was increased by $CaCl_2\;(1.8{\times}10^{-3}\;M)$, but the inhibitory effect of ethanol on the contraction persisted even under the treatment with $CaCl_2$. 3) At higher concentrations, ethanol caused tonic contraction of both preparations from the fundus, the corpus and the antrum in a dose-dependent manner. The tonic contraction of the fundus produced by ethanol was not influenced by $CaCl_2$ or verapamil, whereas the tonic contraction was not produced by ethanol in tile Ca-free solution. 4) Frequency of gastric slow wave was decreased dose-dependently by the addition of ethanol (0.25-1.0%), and tile slow wave was not produced by higher concentration of ethanol (2.0%). 5) The frequency of slow wave was significantly reduced by verapamil only and the inhibitory influence of ethanol on the slow wave frequency was reinforced by verapamil. 6) The treatment of $CaCl_2$ increased significantly the slow wave frequency, and attenuated the inhibitory effect of ethanol on the frequency. It is therefore suggested that ethanol regulates the phasic contraction and the production of slow wave by interfering with the transport of calcium in the stomach muscle of the cat.
Objective: The aim of this experimental study was to evaluate the effects of direct electrical current stimulation (DECS) on bone regeneration in response to an expansion of the inter-premaxillary suture in the rat. Methods: Sixteen 50 - 60 days old Wistar male rats were separated into two equal groups (control and experimental). Both groups were subjected to expansion, and 30-gram of force was applied to the maxillary incisors with helical-spring. In the experimental group, two metallic-screws were placed at lateral parts of the maxillary segments. Electrodes were connected to the screws. The device was activated with current adjustment to measure $10{\mu}A$ continuously and the current was monitored daily during the expansion and early-retention phase. Bone regeneration in the sutural area was histomorphometrically evaluated including new-bone area (${\mu}m^2$), bone perimeter (${\mu}m$), feret's diameter (${\mu}m$) and newly formed bone (%) parameters. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p < 0.05 level. Results: Statistical analysis showed significant differences between groups for all investigated histomorphometric parameters. New bone area (p = 0.002), bone perimeter (p = 0.004), feret's diameter (p = 0.002) and newly formed bone percentage (p = 0.002) measurements were significantly higher in the experimental group than the control group. Bone histomorphometric measurements revealed that bone architecture in the DECS group was improved. Conclusions: The application of DECS to an orthopedically expanded inter-premaxillary suture area during the early retention phase stimulated the formation of new bone.
This research was designed to investigate how the exercise program affects paraplegic standing and walking employing functional electrical stimulation(FES). Emphasis was also given to fatigue of major lower extremity muscles induced by different types of electrical stimulation. We applied continuous and intermittent rectangular pulse trains to quadriceps of 10 normal subjects and 4 complete paraplegic patients. The frequencies were 20Hz and 80Hz, and the knee angle was fixed at 90$^{\circ}$and 150$^{\circ}$to investigate how muscle fatigue is related to muscle length. The knee extensor torque was measured and monitored. We have been training quadriceps and gastrocnemius of a male paraplegic patient by means of electrical stimulation for the past two year. FES standing was initiated when the knee extensors became strong enough to support the body weight, and then the patient started FES walking utilizing parallel bars and a walker. We used an 8-channel constant-voltage stimulator and surface electrodes. The experimental results indicated that paralyzed muscles fatigued rapidly around the optimal length contrary to normal muscles and confirmed that low frequency and intermittent stimulation delayed fatigue. Our exercise program increased muscle force by approximately 10 folds and decreased the fatigue index to half of the initial value. In addition, the exercise enabled the patient to voluntarily lift each leg up to 10cm, which was of great help to the swing phase of FES walking. Both muscle force and resistance to fatigue were significantly enhanced right after the exercise was applied every day instead of 6 days a week. Up to date, the patient can walk for more than two and half minutes at 10m/min while controlling the on/off time of the stimulator by pushing the toggle switch attached to the walker handle.
Journal of Korean Society of Environmental Engineers
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v.36
no.4
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pp.295-302
/
2014
This study demonstrates a double-path CDI as an alternative of advanced wastewater treatment process. While the CDI typically consists of many pairs of electrodes connected in parallel, the new double-path CDI is designed to have series flow path by dividing the module into two stages. The CFD model showed that the double-path had uniform flow distribution with higher velocity and less dead zone compared with the single-path. However, the double-path was predicted to have higher pressure drop(0.7 bar) compared the single-path (0.4 bar). From the unit cell test, the highest TDS removal efficiencies of single- and double-path were up to 88% and 91%, respectively. The rate of increase in pressure drop with an increase of flow rate was higher in double-path than single-path. At 70 mL/min of flow rate, the pressure drop of double-path was 1.67 bar, which was two times higher than single-path. When the electrode spacing was increased from 100 to $200{\mu}m$, the pressure drop of double-path decreased from 1.67 to 0.87 bar, while there was little difference in TDS removal. When proto type double-path CDI was operated using sewage water, TDS, $NH_4{^+}$-N, $NO_3{^-}$-N and $PO_4{^{3-}}$-P removal efficiencies were up to 78%, 50%, 93% and 50%, respectively.
Objective: The purpose of this study was to investigate the local stability of the lower extremity joints and muscle activation patterns of the lower extremity during walking between falling and non-falling group in the elderly women. Method: Forty women, heel strikers, were recruited for this study. Twenty subjects (age:72.55±5.42yrs; height:154.40±4.26cm; mass:57.40±6.21kg; preference walking speed:0.52±0.17m/s; fall frequency=1.70±1.26 times) had a history falls(fall group) within two years and Twenty subjects (71.90±2..90yrs; height:155.28±4.73cm; mass:56.70±5.241kg; preference walking speed: 0.56±0.13m/s) had no history falls(non-fall group). While they were walking on a instrumented treadmill at their preference speed for a long while, kinematic and EMG signals were obtained using 3-D motion capture and wireless EMG electrodes, respectively. Local stability of the ankle and knee joint were calculated using Lyapunov Exponent (LyE) and muscles activation and their co-contraction index were also quantified. Hypotheses were tested using one-way ANOVA and Mann-Whitey. Spearman rank was also used to determine the correlation coefficients between variables. Level of significance was set at p<.05. Results: Local stability in the knee joint adduction-abduction was significantly greater in fall group than non-fall group(p<.05). Activation of anterior tibials that acts on the foot segment dorsal flexion was greater in non-fall group than fall group(p<.05). CI between gastrocnemius and anterior tibials was found to be significantly different between two groups(p<.05). In addition, there was significant correlation between CI of the leg and LyE of the ankle joint flexion-extention in the fall group(p<.05). Conclusion: In conclusion, muscles that act on the knee joint abduction-adduction as well as gastrocnemius and anterior tibials that act on the ankle joint flexion-extention need to be strengthened to prevent from potential fall during walking.
Shin, Kook-Sik;Lim, Woo-Jin;Lee, Sang Eun;Lee, Jae Seon;Cha, Geun Sig
Korean Journal of Soil Science and Fertilizer
/
v.42
no.6
/
pp.513-521
/
2009
The primary goal of this research was to develop an optimized analytical procedure for soil analysis based on ion-selective microelectrodes for agricultural purposes, which can perform on-site measurement of various ions in soil easily and rapidly. For the simple and rapid on-site diagnosis, an analysis of soil chemicals was performed employing a multicomponent-in-situ-extractant and an evaluation of ionselective microelectrodes were conducted through the regressive correlation method with a standard analytical approach widely employed in this area. Examination of sensor responses between various soil nutrient extractants revealed that 0.01M HCl and 1M LiCl provided the most ideal Nernstian response. However, 1M LiCl deteriorated the selective response for analytes due to high concentration (1M) of lithium cation. Thus, employing either 0.1M HCl as an extractant followed by 10 times dilution, or 0.01M HCl as an extractant without further dilution was chosen as the optimal extractant composition. A study of regressive correlation between results from ion-selective microelectrodes and those from the standard analytical procedure showed that analyses of $K^+$, $Na^+$, $Ca^{2+}$, and $NO_3{^-}$ showed the excellent consistency between two methods. However, the response for $NH_4{^+}$ suffered the severe interference from $K^+$. In addition, the selectivity for $Mg^{2+}$ over $Ca^{2+}$ was not sufficient enough since available ionophores developed so far do not provide such a high selectivity for $Mg^{2+}$. Therefore, as an agricultural on-site diagnostic instrument, the device in development requires further research on $NH_4{^+}$ analysis in the soil sample, development of $Mg^{2+}$-selective ionophore, and more detailed study focused on potassium, one of the most important plant nutrients.
A U.S EPA Synthetic soil matrix was used for reference neat soil and pyrene contaminated soil. For the contaminated soil, 4.79 wt.% pyrene was dissolved completely into the djchlorornethane, and the soil was evenly soaked with the pyrene solution. The contaminated soil samples(50$\pm$0.5mg) were heated in a modified electrical screen heater reactor which consisted of a thin stainless foil (3.5cm$\times$13cm$\times$0.00254cm, 302 stainless steel shim), two electrodes, and a 20cm dia. $\times$30cm tall cylindrical Pyrex chamber sealed at both ends by aluminum flanges. The heating rate and time conditions were selected as $455^{\circ}C$ @ $1137^{\circ}C$ /s, $760^{\circ}C$ @ $950^{\circ}C$ /s and $977^{\circ}C$ @ $977^{\circ}C$/s. Tar samples after heating the soils were collected on the aluminum foil funnel and a glass filter paper (25mm dia. filter paper) The tar sample and remnant soil on the reactor were extracted with dichloromethane covering the filters, foils and soil by sonicating each in the waterbath for 10 minutes. The extractions were run on a HPLC. At the low peak temperature(about $455^{\circ}C$ @ $1137^{\circ}C$/s) the color of tar was "white", at the middle peak temperature (about 76$0^{\circ}C$ @ 95$0^{\circ}C$/s) the color of tar was "pink brown", at the high peak temperature (about 977$^{\circ}C$ @ 977$^{\circ}C$/s) the color of tar was "dark brown". Cyclopeta(cd)pyrene (CPEP) , which is an interesting species due to mutagenic effect on human cells, was detected in tar samples only above the middle peak temperature. This species was not detected at the low peak temperature. Six isomers of bipyrene were detected. Phenanthrene(C$_{14}$$H_{10}$) and cyclopenta(def)phenanthrene(C$_{15}$$H_{10}$) were also detected, but their content was very small relative to the other listed compounds.to the other listed compounds.
Suh Chang Ok;Loh. John J.K.;Shin Hyun Soo;Lee Hyung Sik;Moon Sun Rock;Seong Jin Sil;Chu Sung Sil;Kim Gwi Eon;Han Eun Kyung;Park Chan Il
Radiation Oncology Journal
/
v.9
no.1
/
pp.37-45
/
1991
In order to assess the effects of radiofrequency-induced local hyperthermia on the normal liver, histopathologic findings and biochemical changes after localized hyperthermia in canine liver were studied. Hyperthermia was externally adminsitered using the Thermotron RF-8 (Yamamoto Vinyter Co., Japan; Capacitive type heating machine) with parallel opposed electrodes. Thirteen dogs were used and allocated into one control group (N=3) and two treatment groups according to the treatment temperature. Group I (N=5) was heated with $42.5\pm0.5^{\circ}C$ 30 minutes, and Group II (N=5) was heated with $45\pm0.5^{\circ}C$ for 15-30 minutes. Samples of liver tissue were obtained through a needle biopsy immediately after hyperthermia and T,14, and 28 days after treatment. Blood samples were obtained before treatment and W, 3,5, 7,14 and 28 days after treatment and examined for SGOT, SGPT and alkaline phosphatase. Although SGOT and SGPT were elevated after hyperthermia in both groups (three of five in each group), there was no liver cell necrosis or hyperthermia related mortality in Group 1. A hydropic swelling of hepatocytes was prominent histologic finding. Hyperthermia with $45^{\circ}C$ for 30 minutes was fatal and showed extensive liver cell necrosis. In conclusion, liverdamage dy heat of $42.5\pm0.5^{\circ}C$ for 30 minutes is reversible, and liver damage by heat of $45\pm0.5^{\circ}C$ for 30 minutes can be fatal or irreversible. However, these results cannot be applied directly to human trial. Therefore, in erder to apply hyperthermic treatment on human liver tumor safely, close obsewation of temperature with proper thermometry is mandatory. Hyperthermic treatment should be confined to the tumor area while sparing a normal liver as much as possible.
Artificial joint replacement is one of the major surgical advances of the 21th century. The primary purpose of a TKA (Total Knee Arthroplasty) is to restore normal knee Auction. Therefore, ideally, a TKA should: (a) maintain the natural leverage of the knee joint muscles to ensure generating adequate knee muscle moments to accomplish daily tasks such as rising from a chair or climbing stairs;(b) allow the same range of motion as an complete knee; and (c) provide adequate knee joint stability. Four individuals (2 peoples after surgery one year and 2 peoples after surgery three years) participated in this study. All they were prescreened for health and functional status by the same surgeon who performed the operations. Two days of accommodation practice occurred prior to the actual strength testing. The isometric strength (KIN-COM III) of the quadriceps and hamstring were measured at 60$^\circ$ and 30$^\circ$ of knee flexion, respectively. During isokinetic concentric testing, the range of motion was between 10$^\circ$ to 80$^\circ$ of knee flexion (stand-to-sit) and extension (sit-to-stand). for a given test, the trial exhibiting maximum torque was analyzed. A 16-channel MYOPACTM EMG system (Run Technologies, Inc.) was used to collect the differential input surface electromyographic (EMG) signals of the vastus medialis (VM), vastus lateralis(VL), rectus femoris (RF) during sit-to-stand and stand-to-sit tests. Disposable electrodes (Blue SensorTM, Medicotest, Inc.) were used to collect the EMG signals. The results were as follows; 1. Less maximum concentric (16% and 21% less for 1 yew man and 3 years mm, respectively) and isometric (12% and 29%, respectively) quadriceps torque for both participants. 2.14% less maximum hamstrings concentric torque for 1 year man but 16% greater torque for 3 years mm. However, 1 year man had similar hamstring isometric peak torque for both knees. 3. Less quadriceps co-contraction by 1 year man except for the VM at 10$^\circ$-20$^\circ$ and 30$^\circ$-50$^\circ$ range of knee flexion.
Kim, Chul;Choi, Hyun;Kim, Chung-Chin;Kim, Jong-Kyu;Kim, Myung-Suk
The Korean Journal of Physiology
/
v.2
no.1
/
pp.79-88
/
1968
For the purpose of assessing the influence of the mamillary bodies on the general activity and stress response, mamillary body·damaged rats(mamillary body group), operated control rats, and normal control animals were prepared and 2 experiments were carried out. The mamillary bodies. were damaged electrolytically by passing 0.3 ma d.c. current through stereotaxically implanted electrodes. The operated control group received the same treatment except passage of current. In the 1st experiment, each subject belonging to the mamillary body group .(9 rats) or the operated control group (13 animals) was put individually into an activity cage across which an infrared beam traversed. The number of beam interruptions by the movement of the subject in the cage was. recorded hourly for 48 hours and was regarded as an index of general activity. In the 2nd experiment, each of the mamillary body group (52 animals), the operated control group (45 rats), and the normal control group (37 animals) was further divided into 4 subgroups and the adrenal ascorbic acid content of one of the 4 subgroups was measured before, another subgroup immediately after, a third subgroup 2 hours after, and the remaining subgroup 4 hours after termination of a cold stress ($-10^{\circ}C$ for 1 hour). Following results were obtained: 1. Though the total activities in 48 hours of the mamillary body group and the operated control group were not significantly different, the activities of the malnillary body group for a few hours. at the beginning of the measurement and also in the evenings of both the 1st and the 2nd experimental days were significantly greater than those of the operated control group. 2. Judged by the significant reduction in adrenal ascorbic acid content, the stress response of the mamillary body group was as marked as that of the operated control or the normal control group-immediately after termination of cold exposure. Recovery from the stress was accelerated significantly in the mamillary body group and insignificantly in the operated control group compared with that of the normal control group, the intergroup difference of the former two groups being insignificant. It was inferred from the above results that the mamillary bodies may exert an inconspicuous inhibitory influence upon the central mechanism of general activity, and that the mamillaryies bodies may not be the main seat of the stress mechanism, although more works are desirable to confirm the results.
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