• 제목/요약/키워드: Two culture

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Production of Tropane Alkaloids by Two-stage Culture of Scopolia parviflora Nakai Adventitious Root

  • Kim, Won-Jung;Jung, Hee-Young;Min, Ji-Yun;Chung, Young-Gwan;Lee, Cheol-Ho;Choi, Myung-Suk
    • 한국약용작물학회지
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    • 제12권5호
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    • pp.372-377
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    • 2004
  • Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with the SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium, respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

근대 동북아시아의 서양건축문화 수용양상에 관한 연구 - 한국.중국.일본의 교회건축을 중심으로 - (A Study on the acceptance phase of western architecture culture in Modern East-North Asia - Focused on church architecture of Modern Korea.China.Japan -)

  • 김승배
    • 건축역사연구
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    • 제7권4호
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    • pp.131-150
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    • 1998
  • Main theme of this study is the acceptance phase of western architectural culture in modern east-north asia through a series of actual survey and typological analysis of existing church buildings in modern Korea. China. Japan. For this study, 126 modern church buildings of Korea, China, Japan are selected. Also, main subject of this study has deal with the side of culture - thought which background of christianity acceptance and acceptance mind of acceptance main-body, culture adaptation and transformation based on characteristic culturalization of Korea, China, Japan. Especially, I would consistently tried to see the acceptance phase of western architectural culture according to differential acceptance mind of acceptance main-body based on characteristic culturalization of three countries. Conclusively, in the point of view of western architectural culture acceptance phase, it could be said that Korean church architecture had characterized as a blending phase of the two eclectic architectural culture. And in the case of china, as a coexistance phase of the two architectural culture with more western style-oriented. On the other hand, Japanese church architecture had characterized as a syncretism phase of the both traditional and western architectural culture, though it is eclectic style-oriented. Therefore, it is thought that different acceptance mind of western culture had main factors caused of differential acceptance phase, when the two architectural culture, the traditional and the western, encounters.

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삼국시대의 견문화의 연구 -고구려와 백제를 중심으로- (A Study of Silk-Culture in the Period of Three-Nation -On point of the relation of Goguryo & Bakje dynasty-)

  • 박일록
    • 대한가정학회지
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    • 제32권3호
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    • pp.217-226
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    • 1994
  • Cloth culture is one of the basic unit of constructing man's culture in a life of human being. One of the fundamental elements to construct cloth culture is the silk-fabric from the ancient time-Sam Han period B.C 2∼4 Korea. This study is to survey the growing aspects of the silk-fabric influence Goguryo & Bakje dynasty. It is an attempt to manifest that how silk-fabric influence through a trade or as means of currency between the two nations at that time. through a trade or as means of currency between the two nations at that time. To certify I use material such as San guo zhi, Hou Han han shu from China Samkuksaki Dong-kuk-tong-gam from Korea and NiHon Shoki from Japen and I concerned the cultural relations of politics economics and socity of the two dynastics so as to prove a relations of politics economics and socity of the two dynastics so as to prove a relative relation between the two. It is reached to the conclusion that silk fabric had been a constituent role at cloth culture at that time of Goguryo & Bakje. It is pointed out that silk-fabric had been an important role to construct the culture of social economic and politic through a trade and as a precious currency.

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인사조직운영 및 문화유형에 관한 비교 연구 : 철도관련 A, B 양사의 기관사 직종을 중심으로 (A Comparative Study on Personnel, Organization Management System and Corporate Culture : Focusing on Drivers of two Railway Firms)

  • 신택현;이동갑
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2003년도 춘계학술대회 논문집
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    • pp.159-164
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    • 2003
  • The purpose of this article is to comparatively depict the organizational effectiveness of two rail firms based on the cognitive response of employees(group of drivers). To attain this purpose, several aspects of current personnel and organization management system and organizational culture are surveyed through questionaire and analyzed by SPSS. The questionaire includes two parts of items. First, it includes items on personnel-related factors such as evaluation system, job, wage, role, leadership and job satisfaction. Second, it includes items on organizational culture model suggested by Rousseau. The major findings are : 1) In general, the cognitive response of drivers in two firms concerning personnel-related aspects is to a certain degree negative. 2) The cognitive response of drivers in two firms concerning culture-related aspects is also awfully negative. 3) Job satisfaction of drivers in the firm A is highly related to role and leadership factors in terms of personnel-related aspects, on the one hand, and to achievement factor in terms of culture, on the other hand. In case of the firm B, job satisfaction of drivers is highly related to personnel evaluation factor as well as role and leadership factors in terms of personnel-related aspects, on the one hand, and to affiliation, power, and bureaucracy factors in terms of culture, on the other hand.

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아든만 연안지역의 복식문화 동질성 연구 -혼 지역과 아라비아 지역의 부르가를 중심으로 - (A Study on Homogeneity of Costume Culture in the Coastal Areas of the Gulf of Aden -Focusing on the Burga of Horn and Arabia -)

  • 김문숙
    • 복식문화연구
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    • 제9권4호
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    • pp.664-676
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    • 2001
  • This study highlights the homogeneity in the heritage of the costume cultures between the Gulf of Aden, a part of the Horn region of Africa and the Arab region of West. Asia. Specifically, a cross-cultural perspective is used to examine the similarities between the two regions based on their (1) geographic living cultures and (2) the use of a costume accessory called Burga-a face veil. The current trend in research on the traditional costume culture of Africa mostly ties art with the traditional costume culture and examines its from an aesthetic or animalism perspective rather than from a cross cultural Perspective. Compared to Previous research in this area this study used literature reviews and Pictorial analysis to analyze costume cultures from a cross-cultural perspective. The Burga, which is worn in the low lands of the Horn region, which is located between the West Asia and African continents, as well as the Arab region, shows a reciprocal cultural exchange between the two regions. Similarities can be found in the shape, design, decorative elements, and the way the Bursa was worn in two regions. Although the Burga as a face veil is only a small part of a costume, it reaffirms the similar living cultures and geographic characteristics of the two regions. As the living culture environment becomes similar around the world, this study should help with cross culture negotiations as well as forther the development of traditional costume research.

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기독병원과 일반병원의 조직문화 특성에 따른 조직성과 분석 (Analysis on Organization Performance Based on Hospital Culture)

  • 김운신;남은우
    • 한국병원경영학회지
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    • 제4권2호
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    • pp.242-265
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    • 1999
  • Our study attempt is to see organizational performance according to the differences between types of hospital cultures. To determine theoretical relationship between the organizational culture and the performance, we select two hospitals in Pusan which are different in the purposes and shapes of establishment. We surveyed their members on a questionnaire based on the type of the organizational culture of the two institutions, analyzed, and review its organizational effectiveness. For the survey with questionnaires, which had been preliminary studied to raise its validity, question forms were distributed to 528 persons in April, 1999 based on the self-responses and recollected within 48 hours. The recollection rate was high(89.96%) and the quantity of questionnaires used for our final analysis was 430(81.44%). The Cronbach Coefficient Alpha of the questionnaires was 0.742. Regarding statistical techniques for analysis of the written materials, dispersion analysis(ANOVA) was adapted to test the organizational effectiveness of the two hospitals having the different organizational cultures, and Pearson Correlation was applied to determine correlations was among all variables. T-test was performed to test organizational effectiveness based on the differences in the extent of sharing the culture, organizational committment and work satisfaction between the two health institutions. From our analysis, we obtain the following conclusions. First, concerning with organizational culture of the two hospital, one of which is a christian hospital and the other is a private foundation hospital, the former is conservative and human-oriented but the latter focuses on renovation and accomplishment. Second, the private establishment has a relatively higher organizational effectiveness that the religious hospital as a result of analyzing the extent of sharing culture, organizational committment and work satisfaction. Third, it has been found that the correlations between the extent of the sharing culture and the organizational committment, the extent and work satisfaction, and the committment and the satisfaction are respectively positive influencing organizational effectiveness, especially work satisfaction. Fourth, cultural factors by which the christian hospital is affected more positively including human relations among its members, belief, its idea of establishment, tradition, work responsibility, power, and wage. On the other hand, factors such as director's leadership, personnel management, wage, hospital regulations and department managers' management ability have been seen as negative influences in order. And fifth, for the private foundation hospital human relations among its members, wages, work responsibility, director's leadership and department managers' management ability were positive in their sequence while wages, personnel management, hospital regulations, welfare and department managers' management ability were considered as negative influences in order. As these results of this study, the higher extent of sharing organizational culture, the more increasing in both organization committment and work satisfaction, the higher the effectiveness. Although it was somewhat difficult to generalize the results whose subjects were the two hospitals only, it was obvious that organizational culture was an important influential factor of organizational effectiveness. It is questionable that the extent of sharing organizational culture, organizational committment and work satisfaction as variables affecting the effectiveness have their validity, but this study has its significance in that it provided an approaching to evaluate the organizational culture of individual hospitals making allowances for such variables related to the general activities in its hospital. We hope the results of the study could be useful for the managerial strategies of the institutions.

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중국 2차원문화 환경에서의 '탄막(弹幕)'현상 ('Barrage' phenomenon in Chinese Two-dimensional cultural environment)

  • 석향란;최은경
    • 만화애니메이션 연구
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    • 통권50호
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    • pp.23-42
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    • 2018
  • 2차원문화(二次元文化)는 현재 중국 콘텐츠 시장의 핵심 키워드이다. 중국에서는 2차원문화에 대한 이용자가 날로 증가하며 대중들의 관심이 고조되고 있다. 또한 콘텐츠 시장에서의 인기로 인해 많은 자본을 투자하고 있는 실정이다. 2차원문화가 주류문화로 부상한 이유는 주류 소비자층의 전환뿐만 아니라, 첫째로는 2차원문화가 ACGN(Animation, Comic, Game, Light Novel) 외에도 영화, 공연, 테마파크 등 많은 영역 까지 아우른 거대한 산업사슬을 형성하고 있는 것, 둘째로는 사회적 환경과 충성도가 높은 특정 수용자들로 인해 더욱 더 많은 사람들이 2차원문화 행렬에 가담하고 있는 것, 셋째로는 문화의 전파성이 강한 등 세 가지 특징으로부터 2차원문화 콘텐츠에 있어서 수용자의 역할을 찾아보았고, PEST(거시환경 분석)를 통하여 정치, 경제, 사회, 기술 등 네 가지 측면으로 중국 2차원문화 환경을 분석하였다. 또한 2차원문화 수용자들의 생태환경과 소통방식이 변화되면서 수용자는 능동적 참여자이자 콘텐츠의 재생산자로 부각되었는데, 이러한 수용자 콘텐츠 이용방식을 '탄막(弹幕)'현상으로 살펴보았다. 따라서 본 연구는 중국 2차원문화 환경에서 수용자 중심의 콘텐츠산업에 대한 적절한 발전 경로를 논의하는 시사점이 있다.

Trophoblastic Vesicle과 Estradiol-$17\beta$의 첨가가 가토배의 발달에 미치는 영향 (Effects of Trophoblastic Vesicle and Estradiol-$17\beta$ on the Development in Vitro of Rabbit Embryos)

  • 오하식;박충생
    • 한국가축번식학회지
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    • 제10권1호
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    • pp.76-82
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    • 1986
  • This experiment was conducted to determine the effects of trophoblastic vesicles (TV) and estradiol-17$\beta$ on the development in vitro of rabbit embryos. Thirty matured female rabbits were treated with PMSG followed by HCG injection and mating. Embryos were recovered with D-PBS (Dulbecco's Phosphate Buffered Saline) after superovulation, and normally developed to two-to four-cell embryos were used in the subsequent in vitro culture. Basal medium was Medium-199 su, pp.emented with 1.5% bovine serum albumin. Embryo on Day 5 after mating (Day 0) was cut into two or three pieces to remove the embryonic disc. Each piece of tissue was cultured for 24 hours at 37$^{\circ}C$ in 0.5 mlMedium-199 in 5% CO2. During culture, peices of trophoblastic tissue changed into spherical vesicles which were used for co-culture. These spheres were called trophoblstic vesicles. Two-to four-cell embryos were cultured for 4 days in Medium-199 in the absence or presence of trophoblastic vesicle, and two-to four-cell embryos cultured with varing concentration (0, 0.1, 1, 10ng/ml) of estradiol-17$\beta$ for 4 dyas. Culture vessels used were watch glass for coculture with trophoblastic vesicles and micortube for estradiol-17$\beta$ infusion. Compared with the Medium-199 alone as basal culture medium, more blastocysts (46.7% vs 15.1%; P<0.01) and morulae (84.4% vs 56.6%; P<0.05) were developed in the co-culture with trophoblastic vesicles. Estradiol-17$\beta$ infused in culture medium was not effective for embryo development to blastocysts (78.3% in control, 50.0% in 0.1ng/ml, 61.5% in 1ng/ml and 64.4% in 10ng/ml) and also to morulae (91.3% in control, 84.2% in 0.1ng/ml, 92.3% in 1ng/ml and 91.1% in 10ng/ml). Compared with the watch glass culture mehotd, more (P<0.01) blastocysts were developed in microtube culture (78.3% vs 56.6%) and more (P<0.01) morulae in microtube culture (91.3% vs 56.6%).

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전통문화축제의 내실화방안 연구 (Developing Proposals for Korean Traditional Culture Festivals)

  • 정달영;박기종
    • 한국연극학
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    • 제48호
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    • pp.549-569
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    • 2012
  • Since 1995, the Local autonomy era has opened, local festivals have been increasing throughout the country. Traditional culture festivals are also increasing. Performing traditional culture festivals is effective for maintenance and transmission of traditional culture as well as creation and nurturing of local culture. They are also effective promoting local economy. Depending on realization level of character, place and costume based on historic research, traditional culture festivals are classified as the "Representation event" or the "Reenactment event". But there are two problems. The first is unexacting classification method for festival types made by Central government yearly. The second is unable to meet one of two goals which are protecting traditional culture and stimulating local economy throughout increasing tourists. The purpose of this study is to suggest improvement of classification method for festival types, and to offer two suggestions for ensure successful local festivals. First of all, I suggest advanced classification method for festival types. For more sophisticated collection process of national festival status, local government department should ensure purpose of festivals, and central government department should add 3 steps to existing process. For example professional committee for judgement of festival types should be founded for consulting of local and central government department. The second suggestion is reinforcement of historic research for the Representation of traditional culture event. The representation of traditional culture should focus on protection of tradition, and could be perfect by continuous historic research. The last suggestion is cooperation with local governments each other for the Reenactment of traditional culture event. The Reenactment of traditional culture should focus on promoting local economy by increasing tourists. So local governments who have similar traditional events should cooperate to get preventing loss of resource and overcome weakness of promoting.

벼 약배양 1단계 및 2단계 배양을 이용한 캘러스 유도 및 식물 재부화율 비교 분석 (Comparative Analysis of Callus Induction and Plant Regeneration Rates Using One-step and Two-step Cultures for Rice Anther Cultivation)

  • 박영희
    • 생명과학회지
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    • 제31권4호
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    • pp.385-388
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    • 2021
  • 벼 육종을 위한 약 배양은 기존 육종 방법을 사용하여 새로운 품종을 개발하는데 최소 6세대에 필요한 시간을 크게 줄여 동형접합자를 신속하게 생산하는 방법이다. 이 약 배양기술은 방법론적 관점에서 더 많은 녹색 식물을 얻을 기회를 제공하며, 약 배양에서 시간과 노력을 절약하는 배양의 효율성을 높이기 때문에 중요하다. 본 연구에서는 배양액과 배양 방법이 다른 1단계와 2 단계 배양의 캘러스 유도율과 녹색 식물 재분화율로 비교하였다. 1단계 배양은 하나의 배지에서 캘러스 유도 및 식물 재생을 허용하는 반면, 2 단계 배양은 두 개의 다른 배지에서 유도 및 식물 재분화가 필요하다. 이 연구에서는 1 단계 및 2 단계 배양으로 벼 꽃밥의 캘러스 유도와 식물 재생률을 비교했다. 캘러스 형성률은 1 단계 배양의 경우 13.0 %, 2 단계 배양의 경우 8.6%로 2 단계 배양보다 1 단계 배양에서 4.4% 더 높았다. 식물 재분화율은 1 단계 배양에서 1.0%, 2 단계 배양에서 3.0 %로 1 단계 배양보다 2단계 배양에서 식물체 재분화율이 3배 더 높았다. 이것은 2 단계 배양이 반수체 생산을 위한 1 단계 배양보다 더 효율적임을 시사한다.