• Journal of Information Management
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• v.30 no.4
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• pp.39-60
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• 1999

Digital library research has attracted much attention. While many research projects are funded by government agencies and national and international bodies, some are run by specific academic and research institutions and libraries. While some digital library projects, such as the ELINOR project in the UK, the first two phases of the eLib(Electronic Libraries) programme in the UK, and the first phase of the DLI(Digital Library Initiative) in the USA, are now over, a number of other projects are currently under-way in different parts of the world. Beginning with the definitions and characteristics of digital libraries proposed by various researchers, this paper provides brief accounts of some major digital library projects that are currently in progress, or are just completed, in different parts of the world. There follows a review of digital library research under 16 major headings. Literature for this review has been identified through a search on the LISA CD-ROM database and a Dialog search on library and information science databases, and the resulting output has been supplemented by a scan of the various Issues of D-Lib Magazine and Ariadne, and the Web sites of various organizations and institutions engaged in digital library research. The review indicates that we have learned a lot through digital library research within a short span of time. However, a number of issues are yet to be resolved. The paper ends with an indication of the research issues that need to be addressed and resolved in the near future in order to bring the digital library from the researcher's laboratory to the real life environment.

• Journal of the Korean Institute of Gas
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• v.7 no.1 s.18
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• pp.19-23
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• 2003

The purpose of this thesis is to investigate the BLEVE probability of LP gas storage tanks which are relatively more dangerous, by the deductive calculating method using the results of Birk's pilot tank test and the required heat capacity of BLEVE. The result that BLEVEs can occur in only above 43.68 percent of liquid filling level under $600^{\circ}C$ of tank pate temperature and $53^{\circ}C$ of inner liquid temperature, was obtained and will be useful for preventing the BLEVE of LP gas storage tanks in fire sites. In addition, this research showed conditions of external leak and fire causing BLEVE, based on 15ton capacity of LP gas tank which has the same specifications as those in Puchon LP gas filling station accident. The result of the calculation is that the minimum pool fire conditions of BLEVE are above 7.2mm equivalent diameter under a liquid release condition and above 17.6mm equivalent diameter under a two-phase release condition. In the end, the result of calculating the pool size corresponding the above conditions using EFFECTS version 2.1, concludes that a minimum of 3.3 meters of diameter and 10.4 meters of height should be needed for BLEVE outbreak.

• The Korean Journal of Pain
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• v.21 no.2
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• pp.119-125
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• 2008

Background: Cerebral blood vessels are innervated by sympathetic nerves from the superior cervical ganglia (SCG), and these nerves may influence the cerebral blood flow. The purpose of the present study was to evaluate the neuroprotective effect of superior cervical sympathetic ganglion block in rats that were subjected to focal cerebral ischemia/reperfusion injury. Methods: Eighty male Sprague-Dawley rats (270-320 g) were randomly assigned to one of two groups (the ropivacaine group and a control group). In all the animals, brain injury was induced by middle cerebral artery (MCA) reperfusion that followed MCA occlusion for 2 hours. The animals of the ropivacaine group received $30{\mu}l$ of 0.75% ropivacaine, and their SCG. Neurologic score was assessed at 1, 3, 7 and 14 days after brain injury. Brain tissue samples were then collected. The infarct ratio was measured by 2.3.5-triphenyltetrazolium chloride staining. The terminal deoxynucleotidyl transferase mediated dUTP-biotin nick-end labeled (TUNEL) reactive cells and the cells showing caspase-3 activity were counted as markers of apoptosis at the caudoputamen and frontoparietal cortex. Results: The death rate, the neurologic score and the infarction ratio were significantly less in the ropivacaine group 24 hr after ischemia/reperfusion injury. The number of TUNEL positive cells in the ropivacaine group was significantly lower than those values of the control group in the frontoparietal cortex at 3 days after injury, but the caspase-3 activity was higher in the ropivacaine group than that in the control group at 1 day after injury. Conclusions: The study data indicated that a superior cervical sympathetic ganglion block may reduce the neuronal injury caused by focal cerebral ischemia/reperfusion, but it may not prevent the delayed damage.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.29 no.2
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• pp.92-108
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• 2017

Present work shows a numerical method to analysis of interaction analysis between solitary wave and onshore bridge. Numerical simulation is carried out by TWOPM-3D (three-dimensional one-field model for immiscible two-phase flows), which is based on Navier-Stokes solver. To do this, the solitary wave is generated numerically in numerical wave channel, and numerical results and experimental results were compared and analyzed in order to verify the applicability of force acting on an onshore bridge. From this, we discussed precisely the characteristics of horizontal and vertical forces (uplift and downward forces) changes including water level and velocity changes due to the variation of solitary wave height, water depth, onshore bridge's location and type, and number of girder. Furthermore, It is revealed that the maximum horizontal and vertical forces acting on the girder bridge show different varying properties according to the number of girder, although each maximum force acting on the girder bridge is proportional to the increasement of incident solitary wave height, and the entrained air in the fluid flow affects the vertical force highly.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.739-747
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• 1997

This study was performed to clarify various conditions on the test of lipoxygenase-l and to establish the application of new test method for varietal improvement of soybeans in order to decrease beany flavors. Potassium borate and Tris were used as buffer and O.1M potassium borate solution showed the best result for the lipoxygenase-l test. In the range of pH 8.5~9.0 of the buffer, 2mM linoleic acid as substrate was effective. For color development, 100$\mu$l of two solutions(KI and starch) were added to the half soybean seed, successively. The substrate solution included linoleic acid was stored safely for 10 days at 4$^{\circ}C$ in refrigerator and for 4 days at room temperature. The best result was as follows; the 1ml of substrate solution[0.1M potassium borate(pH 9.0), 0.1％ Tween-20, 2mM linoleic acid] was added to the chipped half soybean seed in l.5ml plastic tube, waited for 15 minutes, and 100$\mu$l of color development solutions(5％ saturated KI in 15％ acetic acid, 1％ starch) were added to the tube, successively. After 4 hours, the purple color was observed in the upper phase of the plastic tube in the presence of lipoxygenase-1 and milky color in absence of lipoxygenase-1. The purple color was stable from 4 to 24 hours. There was no interfering effect by lipoxygenase-2 and -3. The plastic tube should be placed in the tube stand without shaking during the lipoxygenase-l test.

• Journal of Microbiology and Biotechnology
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• v.16 no.10
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• pp.1583-1590
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• 2006

T4 endonuclease V (T4 endo V) [EC 3. 1. 25. 1], found in bacteriophage T4, is responsible for excision repair of damaged DNA. The enzyme possesses two activities: a cyclobutane pyrimidine dimer DNA glycosylase (CPD glycosylase) and an apyrimidic/apurinic endonuclease (AP lyase). T4 denV (414 bp cDNA) encoding T4 en do V (138 amino acid) was synthesized and expressed using either an expression vector, pTriEx-4, in E. coli or a baculovirus AcNPV vector, pBacPAK8, in insect cells. The recombinant His-Tag/T4 endo V (rHis-Tag/T4 endo V) protein expressed from bacteria was purified using one-step affinity chromatography with a HiTrap Chelating HP column and used to make rabbit anti-His-Tag/T4 endo V polyclonal antibody for detection of recombinant T4 endo V (rT4 endo V) expressed in insect cells. In the meantime, the recombinant baculovirus was obtained by cotransfection of BacPAK6 viral DNA and pBP/T4 endo V in Spodoptera frugiperda (Sf21) insect cells, and used to infect Sf21 cells to overexpress T4 endo V protein. The level of rT4 endo V protein expressed in Sf21 cells was optimized by varying the virus titers and time course of infection. The optimal expression condition was set as follows; infection of the cells at a MOI of 10 and harvest at 96 h post-infection. Under these conditions, we estimated the amount of rT4 endo V produced in the baculovirus expression vector system to be 125 mg/l. The rT4 endo V was purified to homogeneity by a rapid procedure, consisting of ion-exchange, affinity, and reversed phase chromatographies, based on FPLC. The rT4 endo V positively reacted to an antiserum made against rHis-Tag/T4 endo V and showed a residual nicking activity against CPD-containing DNA caused by UV. This is the first report to have T4 endo V expressed in an insect system to exclude the toxic effect of a bacterial expression system, retaining enzymatic activity.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.5
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• pp.548-555
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• 2007

The control of wastewater treatment has two merits; one is to regulate water quality of effluent and the other is to reduce the cost of wastewater treatment. The purpose of this study was to control the ammonium nitrogen in effluent that is known to cause eutrophication. The control was based on simplified ASM No. 1 which had 3 component materials and 8 coefficients, and the control method was as following. Firstly the ammonium concentration of inflow was measured and the optimal aeration time in effluent was determined according to simplified ASM No. 1 to be 1.0 mg/L. If ammonium concentration of effluent was not equal to 1.0 mg/L, the influent ammonium was corrected by adaptive control. These processes above were repeatedly performed. The SBR running aerobic-anoxic phase had been controlled for 1 month with this method. As a result, the ammonium concentration of the effluent showed in the range of $0.22\sim3.1$ mg/L with an average concentration of 1.1 mg/L. The adaptive control method used in this study was found very useful to control and predict the effluent concentration of ammonium.

• Economic and Environmental Geology
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• v.29 no.6
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• pp.677-687
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• 1996

The Buckchang deposits which is located in the Ockcheon metamorphic zone, are emplaced along $N20-30^{\circ}E$ trending fissure sets. So it is a sort of fissure-filling ore deposits. The results of mineral paragenetic studies suggest two stages of hydrothermal mineralization; stage I: base-metal sulfides stage, stage II: late base-metal sulfides, electrum and silver-bearing sulfosalts stage. The silver-bearing sulfosalts occured as the Buckchang mine are mainly argentite and, minor of canfieldite, tetrahedrite, etc. Au:Ag ratios of the electrums show a highly limited range of nearly 1:1 in atomic %. The temperature, salinity and pressure of the Buckchang deposits estimated from fluid inclusion and sulfur isotope studies are as follows; stage I: $174{\sim}250^{\circ}C$, 0.35~4.01 NaCl eq. wt.%, 0.40~1.00 Kbar, stage II: $138{\sim}222^{\circ}C$, 1.9~8.4 NaCl eq. wt.%, 0.22~0.53 Kbar. The estimated oxygen and sulfur fugacity during stage I mineralization, based on phase relation of associated minerals, range from $10^{-39.7}{\sim}10^{-44.7}$ atm. and $10^{-13.4}{\sim}10^{-18.1}$ atm., respectively. All these evidences suggest that the Buckchang deposits are polymetallic epithermal ore deposits.

• Journal of Animal Science and Technology
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• v.46 no.5
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• pp.871-878
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• 2004

Pulsed electric fields(PEF) technology is one of the latest nonthermal methods of food processing for obtaining safe and minimally processed foods. This technology can be effectively explored for obtaining safe food with minimum effect on nutritional, flavor, rheological and sensory qualities of food products. The process involves the application of high voltage(typically 20 ${\sim}$ 80 kv/cm) to foods placed between two electrodes. The mode of inactivation of microorganism; by PEP processing has been postulated in term; of electric breakdown and electroporation. The extent of destruction of microorganisms in PEF processing depends mainly on the electric field strength of the pulses and treatment time. For each cell types, a specific critical electric field strength and specific critical treatment time are required depending on the cell characteristics and the type and strength of the medium where they have been present. The effect also depends on the types of microorganisms and their phase of growth. A careful combination of processing parameters has to be selected for effective processing. The potential applications of PEF technology are numerous ranging from biotechnology to food preservation. With respect to food processing, it has already been established that, the technology is non-thermal in nature, economical and energy efficient, besides providing minimally processed foods. This article gives a brief overview of this technology for food processing applications.

• The Korean Journal of Pharmacology
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• v.20 no.2
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• pp.35-47
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• 1984

Contents of immunoreactive ${\beta}-endorphin$ and maximum of $^3H-morphine$ binding was measured in the rat midbrain homogenates from different subgroups at 24 hour interval over 24 hours. Animals were adapted to the light-dark cycle(L : D, 12: 12) or constant darkness (D : D, 12 : 12) for 3 weeks. After the adaptation, 0.5ml of physiologic saline or drug was administered twice a day for 2 weeks. A highly significant circadian rhythm with the peak$(94.8{\pm}7.7\;fmole/mg\;protein)$ at 06:00 and the nadir $(27.6{\pm}2.4\;fmole/mg\;protein)$ at 18:00 was observed in constant of group. Constant dark or treatment of reserpine, pargyline, imipramine, amphetamine and chlorpromazine modified the diurnal rhythm in the time of peak and nadir, shape, phase amplitude and 24 hour mean of ${\beta}-endorphin$ contents. Opiate receptor binding by $^3H-morphine$ also showed highly significant diurnal change in control and constant dark adapted rats. Statistical analysis by one-way analysis of variance and two-way analysis of variance indicates that the·re are highly significant differences between the diurnal change of ${\beta}-endorphin$ in control and those constant dark adapted and drug treated groups. However diurnal change of Maximum $^3H-morphine$ binding is closely related to the change of ${\beta}-endorphin$ contents. The results are interpreted with regard to the circadian rhythm of beta-endorphin contents, its modification by psychoactive drugs and possible mechanism of diurnal change of opiate receptor in brain.