• Biomolecules & Therapeutics
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• 제28권5호
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• pp.405-413
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• 2020

Fibroblast-like synoviocytes (FLS) play a crucial role in initiating rheumatoid arthritis. B-cell activating factor (BAFF) plays a role in FLS survival as well as in B cell maturation and maintenance. Here, we investigated whether tumor necrosis factor (TNF)-α-induced BAFF expression controls FLS migration and whether BAFF expression in FLS could be regulated by KR33426 which is the inhibitor of BAFF binding to BAFF receptors (BAFF-R) by using MH7A synovial cells transfected with the SV40 T antigen. More TNF-α-treated cells migrated compared to the control. TNF-α increased BAFF expression in FLS, significantly. FLS migration was inhibited by the transfection with BAFF-siRNA. KR33426 also inhibited BAFF expression increased by TNF-α treatment in FLS as judged by western blotting, PCR, and transcriptional activity assay. Kinases including JNK, p38 and Erk were activated by TNF-α treatment. While JNK and p38 were inhibited by KR33426 treatment, no changes in Erk were observed. Transcription factors including p65, c-Fos, CREB and SP1 were enhanced by TNF-α treatment. Among them, c-Fos was inhibited by KR33426 treatment. Small interference(si)-RNA of c-fos decreased BAFF transcriptional activity. FLS migration induced by TNF-α was inhibited by the transfection with BAFF-siRNA. KR33426 increased Twist, Snail, Cadherin-11 and N-Cadherin. In contrast, KR33426 decreased E-cadherin and TNF-α-enhanced CCL2. Taken together, our results demonstrate that synovial cell migration via CCL2 expression could be regulated by BAFF expression which is decreased by KR33426 and c-Fos-siRNA. It suggests for the first time that the role of BAFF-siRNA on FLS migration might be matched in the effect of KR33426 on BAFF expression.

• Advances in nano research
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• 제12권5호
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• pp.529-547
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• 2022

Graphene is one of the strongest, stiffest, and lightest nanoscale materials known to date, making it a potentially viable and attractive candidate for developing lightweight structural composites to prevent high-velocity ballistic impact, as commonly encountered in defense and space sectors. In-plane twist in bilayer graphene has recently revealed unprecedented electronic properties like superconductivity, which has now started attracting the attention for other multi-physical properties of such twisted structures. For example, the latest studies show that twisting can enhance the strength and stiffness of graphene by many folds, which in turn creates a strong rationale for their prospective exploitation in high-velocity impact. The present article investigates the ballistic performance of twisted bilayer graphene (tBLG) nanostructures. We have employed molecular dynamics (MD) simulations, augmented further by coupling gaussian process-based machine learning, for the nanoscale characterization of various tBLG structures with varying relative rotation angle (RRA). Spherical diamond impactors (with a diameter of 25Å) are enforced with high initial velocity (V_i) in the range of 1 km/s to 6.5 km/s to observe the ballistic performance of tBLG nanostructures. The specific penetration energy (E_p^*) of the impacted nanostructures and residual velocity (V_r) of the impactor are considered as the quantities of interest, wherein the effect of stochastic system parameters is computationally captured based on an efficient Gaussian process regression (GPR) based Monte Carlo simulation approach. A data-driven sensitivity analysis is carried out to quantify the relative importance of different critical system parameters. As an integral part of this study, we have deterministically investigated the resonant behaviour of graphene nanostructures, wherein the high-velocity impact is used as the initial actuation mechanism. The comprehensive dynamic investigation of bilayer graphene under the ballistic impact, as presented in this paper including the effect of twisting and random disorder for their prospective exploitation, would lead to the development of improved impact-resistant lightweight materials.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1245-1252
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• 2022

Induced pluripotent stem cells (iPSCs) can be generated from somatic cells using Oct4, Sox2, Klf4, and c-Myc (OSKM). Small molecules can enhance reprogramming. Licochalcone D (LCD), a flavonoid compound present mainly in the roots of Glycyrrhiza inflata, acts on known signaling pathways involved in transcriptional activity and signal transduction, including the PGC1-α and MAPK families. In this study, we demonstrated that LCD improved reprogramming efficiency. LCD-treated iPSCs (LCD-iPSCs) expressed pluripotency-related genes Oct4, Sox2, Nanog, and Prdm14. Moreover, LCD-iPSCs differentiated into all three germ layers in vitro and formed chimeras. The mesenchymal-to-epithelial transition (MET) is critical for somatic cell reprogramming. We found that the expression levels of mesenchymal genes (Snail2 and Twist) decreased and those of epithelial genes (DSP, Cldn3, Crb3, and Ocln) dramatically increased in OR-MEF (OG2^+/+/ROSA26^+/+) cells treated with LCD for 3 days, indicating that MET effectively occurred in LCD-treated OR-MEF cells. Thus, LCD enhanced the generation of iPSCs from somatic cells by promoting MET at the early stages of reprogramming.

• 대한통합의학회지
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• 제10권4호
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• pp.93-102
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• 2022

Purpose : The purpose of this study was to analyze the change in thickness of transvers abdominis, internal oblique, and external oblique when virtual reality based ring fit adventure is applied to young adults in order to investigate the effect of ring fit adventure on core stabilization. Methods : 30 subjects participated in the experiment. Subjects were randomly assigned to two groups. 15 subjects performed ring fit adventure core exercise (experimental group) and 15 subjects bridge and dead bug exercise (control group). The ring fit adventure core exercise program consists of 6 types, 1) bow pull, 2) overhead lunge twist, 3) pendulum bend, 4) seated ring raise, 5) plank, 6) warrior III pose. Each exercise was performed for 5 minutes, for a total of 30 minutes. The bridege and dead bug exercise were performed for 15 minutes each for a total of 30 minutes. All interventions were performed 3 times a week for 4 weeks. Thickness of the abdominal muscles was measured with a ultrasound. The paired t-test was used to compare the thickness of the transverse abdominis, internal oblique, and external oblique before and after intervention, and the comparison between groups was analyzed using the independent t-test. Results : As a result, in the experimental group, thickness of transverse abdominis and internal oblique increased significantly (p<.05), but external oblique decreased significantly (p<.05), and in the control group, thickness of transverse abdominis, internal oblique, and external oblique increased significantly (p<.05). There was a significant difference in external oblique in the difference between groups (p<.05). Conclusion : These study results showed that core exercise using ring fit adventure can reduce external oblique and increased selective muscle activity of transverse abdominis and internal oblique of the deep abdominal muscles, so it is meaningful as an effective intervention for core stabilization.

• 복식문화연구
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• 제31권5호
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• pp.640-650
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• 2023

This study aimed to suggest a suitable collar pattern by visually evaluating the appearance of the amount of collar drape by the starting position of the lapel line of a double-breasted tailored jacket using a 3d virtual fitting program. It created an avatar based on the mean size of women in their 20s (the 8th Size Korea) using clo network (double fastening: 10cm, collar width: 4.5cm, collar stand: 3cm, and lapel width: 8.5cm). The starting of the lapel twist line was waistline level, the 1/2 level of bustline and waistline, or bustline level, and collar laying amount was 4.5, 5.5, 6.5, or 7.5cm. It was evaluated by garment construction experts using 5, 6, and 4 items on the front, sides, and back, respectively. Descriptive statistics, F-test, Duncan-test, and reliability analysis were conducted using SPSS 22. When collar laying amount was 6.5cm, it was best rated regardless of the starting point. Under waist line, when collar laying amount was 6.5cm, it was best rated regardless of the starting point. When collar laying amount was large, the collar's outline length increased, resulting in unnecessary wrinkles from the neckline to the lapel, affecting the overall collar appearance. When collar laying amount was the smallest, the collar was lifted and the width was narrowed, exposing the seam connecting the collar and neckline. The length of the collar's outline varied depending on collar laying amount, which was important to make the outline sit comfortably on the body.

• 대한치과교정학회지
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• 제33권2호
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• pp.131-140
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• 2003

본 연구는 전기도금을 이용하여 각형 선재의 굵기를 증가시킨 후 기존의 선재와 물성을 비교함으로써 이의 임상적 적용 가능성을 알아보고자 시행되었다. 한 변의 길이가 0.016 인치인 정사각형 스테인레스 스틸 교정용 선재에 니켈 전기도금을 시행하여 굵기를 0.001 인치 증가시켜 한 변의 길이가 0.017인치인 정사각형 선재로 만들었고 도금층의 밀착성을 증진시키기 위해 $400^{\circ}C$의 전기로에서 10분간 열처리를 시행하였다 시편의 물성변화를 알아보기 위하여 전기도금을 이용하여 제작된 15개의 시편을 실험군(016P군)으로, 기존의 0.016 인치 (016군), 0.017 인치 (017군) 스테인레스 스틸 교정용 선재를 대조군으로 설정한 후 3점 굴곡 시험과 비틀림 시험을 시행하여 다음과 같은 결과를 얻었다. 1. 3점 굴곡 시험 결과 0l6P군이 016군에 비해 강성과 항복강도, 극한강도 모두 증가하는 경향을 보였으며, 강성과 극한강도는 통계적으로 유의한 차이가 있었다(p<0.05). 2. 3점 굴곡 시험 결과 0l6P군이 017군보다 강성과 항복강도, 극한강도 모두 낮은 경 향을 보였으며, 강성은 통계적으로 유의한 차이가 있었다(p<0.05). 3. 비틀림 시험 결과 016P군이 016군에 비해 비틀림 강성과 항복 비틀림 모멘트, 최대 비틀림 모멘트 모두 높았으며, 통계적으로 유의한 차이가 있었다(p<0.05). 4. 비틀림 시험 결과 016P군이 017군에 비해 비틀림 강성과 항복 비틀림 모멘트, 최대 비틀림 모멘트 모두 낮은 경향을 보였으며, 이 중 항복 비틀림 모멘트와 최대 비틀림 모멘트에서 통계적으로 유의한 차이가 있었다(p<0.05). 이상의 결과를 요약해 보면 각형 선재에 도금을 시행하여 굵기를 증가시키고 물성이 증가될 수 있음을 알았다. 비록 도금을 시행한 016P군이 기존의 017군보다 강성이 낮았으나 이는 반대로 016P군이 017군보다 탄성이 높음을 나타내며 토크조절이 부족한 경우 브라켓 슬롯에 쉽게 삽입되어 교정력을 발휘할 것으로 기대된다.

• 한국잡초학회지
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• 제15권1호
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• pp.30-38
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• 1995

재배양식별(栽培樣式別)로 발생전토양표면처리(發生前土壤表面處理)된 pyrazolate는 처리후(處理後) 10일째 피에서는 건답조건(乾畓條件)의 경우 twist현상(現像)이, 담수조건(湛水條件)에서는 백화현상(白化現像)이 현저하게 나타났다. 처리후(處理後) 20일째 표면직파(表面直播) 벼의 초장(草長) 및 근장(根長)은 무처리(無處理)와 유사하였으나, 토중(土中) 직파(直播)벼는 근장(根長)보다 초장(草長)이 더 억제(抑制)되었으며 피는 벼보다 심하게 억제(抑制)되었다. 담수조건(湛水條件)에서는 건답조건(乾畓條件)보다 더 심한 억제경향(抑制傾向)을 보였으나 벼와 피간의 뚜렷한 차이(差異)는 인정(認定)되지 않았다. 한편 이앙(移秧)벼의 초장(草長)과 근장(根長)은 무처리(無處理)에 비해 증가(增加)되는 생장(生長)을 보였다. 표면직파(表面直播)벼의 지상부(地上部) 및 지하부(地下部) 생체중(生體重)은 무처리(無處理)에 비해 오히려 증가(增加)되었고, 토중직파(土中直播)벼는 무처리(無處理)와 유사한 생장(生長)을 보였으나 피는 무처리(無處理)에 비해 각각 42% 및 41% 억제(抑制)되었다. 그러나 담수조건(湛水條件)의 표면직파(表面直播)벼에서는 지하부생장(地下部生長)이 치명적으로 억제(抑制)되었고 피는 완전히 생장(生長)이 억제(抑制)되었다. 이앙(移秧)벼는 무처리(無處理)와 유사한 생장(生長)을 보였다. 해부학적(解剖學的) 반응(反應)에 있어서 건답직파조건(乾畓直播條件)의 피에서만 엽록소(葉綠素) 파괴(破壞)에 의한 엽육조직(葉肉組織)의 위축(萎縮)과 엽초 및 엽시원체(葉始原體)의 위축(萎縮)으로 인한 두께감소가 관찰되었으나, 담수(湛水) 및 이앙조건(移秧條件)에서 벼와 피는 뚜렷한 해부학적(解剖學的) 변화(變化)는 관찰되지 않았다.

• BMB Reports
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• 제52권10호
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• pp.595-600
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• 2019

Cardiac fibrosis is a common feature in chronic hypertension patients with advanced heart failure, and endothelial-to-mesenchymal transition (EndMT) is known to promote Angiotensin II (Ang II)-mediated cardiac fibrosis. Previous studies have suggested a potential role for the transcription factor, ETS-1, in Ang II-mediated cardiac remodeling, however the mechanism are not well defined. In this study, we found that mice with endothelial Ets-1 deletion showed reduced cardiac fibrosis and hypertrophy following Ang II infusion. The reduced cardiac fibrosis was accompanied by decreased expression of fibrotic matrix genes, reduced EndMT with decreased Snail, Slug, Twist, and ZEB1 expression, as well as reduced cardiac hypertrophy and expression of hypertrophy-associated genes was observed. In vitro studies using cultured H5V cells further confirmed that ETS-1 knockdown inhibited $TGF-{\beta}1$-induced EndMT. This study revealed that deletion of endothelial Ets-1 attenuated Ang II-induced cardiac fibrosis via inhibition of EndMT, indicating an important ETS-1 function in mediating EndMT. Inhibition of ETS-1 could be a potential therapeutic strategy for treatment of heart failure secondary to chronic hypertension.

• 수산해양기술연구
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• 제36권2호
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• pp.73-88
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• 2000

쌍끌이중층망은 1993년에 우리 나라에 처음으로 도입된 이후 그 보급이 급속하게 확산되어 현재는 대부분의 온 쌍끌이 기선저인망어선이 중층망을 사용하고 있다. 그런데, 이 어구를 운용함에 있어서 망목의 크기가 12,800mm의 것으로 커지면서 어구현황 및 전개성능에 대한 충분한 연구가 이루어지지 않아서 대상어종 및 해양환경에 따른 어구어법과 운용기술의 정립이 미흡하고, 그물목줄의 가닥수가 많으며 뜸이 그물코에 끼여서 파망이나 안전사고의 원인이 된다는 점이 문제점으로 지적되고 있다. 이와 같은 문제점을 해결하기 위하여 현재 가장 많이 사용되고 있는 최대망목 12,800mm인 중층망의 구조적인 결함을 보완하여 설계한 개량형의 모형망을 제작하여 회류수조에서 그물목줄의 길이와 가닥수 및 침강력의 변화에 따른 어구의 전개성능을 실험한 결과를 요약하면 다음과 같다. 1. 그물목줄의 길이는 옆줄에 연결된 2번과 3번 목줄의 길이를 현행의 것에 비하여 약 30%를 더 짧게 한 어구의 전개성능이 약 7% 향상되었다. 2. 그물목줄의 가닥수는 4가닥형이 가장 우수하며, 3가닥형, 2가닥형의 순으로 전개성능이 저하되었다. 특히, 2가닥형의 경우에는 유속이 빨라짐에 따라 옆판의 중앙부가 뒤쪽으로 뚜렷하게 처지는 것이 관찰되었다. 3. 침강력을 크게 할수록 어구의 전개성능은 우수해지며, 그물목줄을 3가닥형, 2가닥형으로 하는 경우는 침강력을 4가닥형일 때의 각각 약 1.5배, 1.6∼l.7배 정도로 크게 해 주어야 기존어구와 같은 전개성능을 나타낼 수 있었다.

• 복식
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• 제62권3호
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• pp.137-151
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• 2012

In 1966, $Seokgatap$ pagoda in $Bulguksa$ temple was damaged by the tomb robbers and was dismantled to fix the damage. In the process, many offerings to Buddha and containers for Sarira(the cremated remains) were found in $Sarigong$(specially designated space for the Sarira casket) inside the second floor of the pagoda. Many fabrics like $Geum$, $Neung$(twill), $Rha$(complex gauze), silk tabby and linen were also excavated. In this study, $Geum$ fabric from the $Seokgatap$ was closely examined. $Geum$ of $seokgatap$ is weft-faced compound weave according to the analysis of its weaving pattern which was wrongly presumed as warp-faced compound weave for some time. Technical analysis of $Geum$: Main: silk, Binding: silk, Proportion: 1 main warp to 1 binding warp, Count: 15 main warps and 15 binding warps per centimeter, Weft: polychrome silk without apparent twist, Colors: yellow, mustard yellow, deep blue, green and purple, Weave: weft-faced compound twill, 1/2 S. $Geum$ of $Seokgatap$ was made in the $8^{th}$ century, since it was weaved in weft-faced compound weave twill which was popular in the $8-9^{th}$ century. And also, the arrangement of the colors was done in the same way of gradation $Geum$ silk which was popular in the $7-8^{th}$ C in China and Japan. Third, we restored the pattern of $Geum$ of the Unified Shilla Dynasty for the first time. It was very difficult to figure out the shape and the size of pattern since the fabric was partially lost and ruined. We tried to draw the diagram of structure with the cross point of the warp and the weft to restore the pattern. By doing so, we could identify two kinds of small flower pattern, palmette and the pattern of repeating vines. Fourth, we could infer that the $Geum$ of $Seokgatap$ was used for $geumdae$(a pouch made of $geum$) by analyzing all the documents and the characteristics of the fabric.