• Journal of Species Research
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• v.9 no.1
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• pp.11-18
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• 2020

Larva and pupae of Prosimulium kiotoense and larvae of Twinnia japonensis were collected and reported from Korea for the first time. The genus Prosimulium, previously unknown from Korea, contains 80 species worldwide and 8 species are known in Japan. Species are separated into 3 species groups; hirtipes species-group (47 spp.), macropyga species-group (14 spp.) and magnum species-group (19 spp.), with which P. kiotoense belongs to the hirtipes species-group. The genus Twinnia, also previously unknown from Korea, contains only 10 species worldwide. In this study, I provide detailed descriptions and photographs of larvae and pupae P. kiotoense and larvae T. japonensis. Prosimulium kiotoense is characterized by pupa with gill of 16 filaments, cocoon amorphous and entirely covers the pupal body, head and thorax densely covered with microtubercles, larva with postgenal cleft apically straight, and hypostomal teeth with median tooth prominent. On the other hand, larva of T. japonensis can be characterized by labral fan absent, gill histoblast with 16 filaments, and hypostomal teeth with median tooth distinctly shorter than other teeth. In addition to descriptions for each species, a key to Korean Prosimulium and Twinnia is provided, with reference to the Japanese species and habitat information.

• Animal Systematics, Evolution and Diversity
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• v.36 no.4
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• pp.285-295
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• 2020

Adults of Prosimulium kiotoense and Twinnia japonensis were collected and reported from Korea for the first time. Since adult black flies are notorious for morphological homogeneity particularly in female, larval and pupal characters have been mainly used to identify them. Adults of P. kiotoense can be identified by the following combination of characteristics: Adult, wing with radial sector (Rs) branched into R²⁺³ and R⁴⁺⁵; hind leg basitarsus without calcipala; first tarsomere without pedisulcus. Female, claw without basal thumblike lobe; hypogenial valve elongate, convex, heavily sclerotized medially, posterior end touching each other, space between valves rhomboid. Male, claw with basal thumblike lobe; ventral plate keel shaped; gonostylus with 2-3 spinules. Adults and pupa of T. japonensis can be identified by the following combination of characteristics: Adult, antenna with 7 flagellomeres. Female, hypogenial valve broad, posterior end of valve not touching each other; cercus elongate, subquadrate; spermatheca slightly wider and long, round. Male, claw with basal thumblike lobe; gonostylus with 1 spinule; ventral plate flat. Pupa, gill of 16 filaments, arising from 3 swollen stalks; abdominal tergites without spine combs except tergites III and IV with small recurved hooks; terminal spine well developed, wavy shaped.