• Title/Summary/Keyword: Twig

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Anti Inflammatory Activity of Viburnum dilatatum Thunb. Extract as Cosmetic Ingredient (화장품 소재로서 가막살나무 추출물의 항염증 효능)

  • Kwon, Yoo-Bin;Yoo, Byoung-Sam;Kim, Dae-Shin;Moon, Seong-Joon;Yoon, Moung-Seok;Park, Soo-Nam
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.36 no.3
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    • pp.183-191
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    • 2010
  • The crude ethanol extracts and their solvent-partitioned fractions derived from the leaf and twig of Viburnum dilatatum Thunb. were investigated for their 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging efficacy. The results showed that the butanol-soluble fraction ($SC_{50}\;=\;110.30\;{\mu}g/mL$) exhibited higher anti-oxidant activity than the crude ethanol extract ($SC_{50}\;=\;117.03\;{\mu}g/mL$) in the DPPH assay model. Then, the effects of the same extract samples on the production of nitric oxide were examined in LPS-stimulated RAW264.7 cells. Although the hexane and methylene chloride-soluble fraction showed a weak anti-oxidant activity, they exhibited potent inhibitory activity of NO production above 50 % at a concentration of $10\;{\mu}g/mL$. The hexane-soluble fraction also showed the inhibitory effect on mRNA expression of pro-inflammatory mediators such an TNF-$\alpha$, IL-$1{\beta}$, IL-6, iNOS and COX-2 in LPS-stimulated RAW264.7. These results suggest that the solvent extracts of Viburnum dilatatum Thunb. could be used as an anti-irritation ingredient.

Field records of Dr. Tchang-Bok Lee based on herbarium specimens deposited at SNUA (서울대학교 농업생명과학대학 수목원 수우(樹友)표본관(SNUA)에 소장된 채집표본을 근간으로 한 이창복교수의 채집기록)

  • Chang, Chin-Sung;Kim, Hui;Jeon, Jeong-Ill
    • Korean Journal of Plant Taxonomy
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    • v.33 no.4
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    • pp.455-472
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    • 2003
  • The period from 1946 until his retirement from Department of Forest Resources, Seoul National University was a time of research and professional activity on many fronts for Korean Plant Taxonomy. From 1952 to 1984, he travelled south Korea and collected more than 70,000 specimens. Working out of this project, Dr. Lee developed the SNUA herbarium (Herbarium of The Arboretum of Seoul National University) as the best University herbarium in Korea for the modem floristic work. Dr. Lee's first interest rests on his contributions to our understanding of the twig identification in early 1950. Asian deciduous oak taxa have attracted particular focus to him late 1950 and early 1960. Accordingly Dr. Lee has been one of the specialists on systematic study of Asian oak which is a basis of identification in eastern Asia now. Dr. Lee's contributions concerning rare and endangered plants in Korea provide a tremendous information after a Japanese taxonomist, T. Nakai did in early 1920 to late 1930 in Korea. During his fourteen year career from 1970 to 1984, Dr. Lee spent time to investigate flora of many local and National Park sites. Much of his effort was directed to the voucher specimens for which he worked for the Illustrated Flora of Korea and also served as a field collector. These collections at SNUA enabled us to extend our educational outreach from the local to the national flora research. Although Dr. Lee has been and continues to be the soul of SNUA, collection records about his purpose and aim for each trip are not well documented except date and field studied sites. This lesson enables us to tackle the same issue of balancing quantity and quality with well documented specimens now.

Studies on the White rot and Blister Canker in Apple Trees caused by Botryosphaeria berengeriana (사과나무의 겹무늬병(윤문병) 및 사마귀병 (우피병)의 병원균과 병원성에 관한 연구)

  • Lee Du Hyung;Yang Jang Suck
    • Korean journal of applied entomology
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    • v.23 no.2 s.59
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    • pp.82-88
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    • 1984
  • Fruit rot and blister canker, a disease of apple occurring severely in Korea has been studied for correct identification of the syndrome In fruit and apple trees. Among the fungi isolated from blister cankers, rough barks or fruits showing rotting of 7 different host species were Botryosphaeria berengeriana (pycnidial stage. Dethiorella mali), Penicillium expansum and Alternaria sp. from apple rots and Phomopsis sp. from pear fruit rots. The most dominant isolates were B. berengeriana. Ten isolates of D. mali were grouped in to two conidial types based up mycelial growth rate, growth habits and mycelial coloration on PDA. None of 10 isolates was chromogenic. Pycnidia in apple stems, stromatic, dark brown, globose or subglobose and the measuring were $103.5-287.5{\mu}\times92.0-287.5\mu$. The pycnidia contained hyaline, nonseptate, fusiform conidia. The sizes of pycnidiospore of isolates obtained from apple twig were $4.3-7.2{\mu}\times20.0-31.5{\mu}(average\;5.9\times25.4\mu)$. Some conidia of this fungus from apple, pear, peach and ornamental cherry showed 1-,2-,3-septate before or during germination. Microconidia were observed in pycnidia on PDA and fruit lesion of inoculated host. Symptoms on leaves and fruits were contoured brown spots when inoculated. Wart-like protuberance were formed on the surface of apple and pear. Canker appeared on branches of peach and ornamental cherry inoculated.

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Evaluation of Cold Tolerance of Blueberry (Vaccinium corymbosum L.) and Diagnosis of Freezing Injury Using Timber Moisture Meter (블루베리의 내한성 평가 및 목재수분계측기를 이용한 동해피해 진단)

  • Kim, Ki-Deog;Lee, Jun-Gu;Ryu, Myeong-Sang;Yoo, Dong-Lim;Kwon, Young-Seok;Lee, Jong-Nam
    • Journal of Bio-Environment Control
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    • v.21 no.4
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    • pp.354-361
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    • 2012
  • This study was conducted to evaluate on the freezing tolerance of introduced blueberry cultivars in Korea and to investigate availability of portable timber moisture meter for simple and rapid diagnosis of blueberry-shoot damage by freezing during wintering. Frost tolerance of blueberry cultivars showed big difference that rates of blueberry-shoot death were widely distributed from about 0% to 100% after wintering. Optical density in TTC reduction of blueberry twig treated low temperature was low in order of $-40^{\circ}C$ < $-21^{\circ}C$ < $4^{\circ}C$. Hardiness evaluation of visible injury in the cross-sectional surface color did not agree with that of rates of blueberry-shoot death during wintering. Lowest water content of blueberry stem measured by timber moisture tester during wintering was about 15%. During wintering, water contents of blueberry stems were higher at lower part of tree, but were low at end part of stems, and then when the blueberry grew again for spring, the water content gradually increased to 20~40%. Water content of blueberry stem with freezing injury during wintering decreased to under 5% by desiccation. Therefore it is assummed that the moisture content of blueberry stem injured by freezing during wintering was about under 14%, and it is expected that portable timber moisture meter could be available for rapid diagnosis of blueberry freezing injury in field.

Numerical analysis of Quercus L. subgenus Quercus (Fagaceae) in Korea (한국산 참나무속 참나무아속(너도밤나무과)의 수리분류학적 분석)

  • Park, Jin Hee;Chung, Myong Gi;Sun, Byung-Yun;Kim, Ki-Joong;Park, Jae-Hong;Park, Chong-Wook
    • Korean Journal of Plant Taxonomy
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    • v.35 no.1
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    • pp.57-80
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    • 2005
  • Morphological variation of Quercus L. subgen. Quercus in Korea was examined by numerical methods including principal components analysis of major morphological characters. Principal components analysis revealed the presence of five major species groups of the subgen. Quercus in Korea; these include 1) a group consisiting of Q. acutissima and Q. variabilis individuals, 2) Q. dentata, 3) Q. aliena, 4) Q. mongolica, and 5) Q. serrata. The putative interspecific hybrid individuals occupied intermediate position between the putative parent species in the PCA plot. The analysis also strongly suggested that size and shape of leaf blade and teeth or lobes, twig diameter, size and shape of nuts, and length and width of scales are very useful in distinguishing the species and the putative hybrids of the subgen. Quercus.

Postprandial hypoglycemic effects of mulberry twig and root bark in vivo and in vitro (In vivo와 in vitro에서 상지 및 상백피 에탄올추출물이 식후 혈당 상승 억제 조절에 미치는 영향)

  • Park, Soo Yeon;Jin, Bo Ra;Lee, Yu Rim;Kim, You Jin;Park, Jeong Bin;Jeon, Young Hee;Choi, Sang Won;Kwon, Oran
    • Journal of Nutrition and Health
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    • v.49 no.1
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    • pp.18-27
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    • 2016
  • Purpose: Our previous study demonstrated the hypoglycemic effects of mulberry (Morus alba L.) leaf and the underlying mechanisms. Here we explored the potency of mulberry twigs (TW) and root barks (RB) in postprandial hypoglycemic effects in vitro and in vivo. Methods: The major components of TW and RB were determined by high performance liquid chromatography (HPLC). Alpha-glucosidase inhibition and glucose/fructose uptake inhibition in Caco-2 cells were determined for TW, RB, and their major components, followed by an oral sugar tolerance test (OSTT) in streptozotocin-induced diabetic rats. Male Wistar rats were fed a high-fat diet for 2 weeks and then a single dose of streptozotocin (35 mg/kg B.W) was administered by intraperitoneal injection. Rats with fasting blood glucose levels above 126 mg/dL were randomly divided into 5 groups (n = 8/group) for the following treatments by gavage for 4 weeks: vehicle (normal control and diabetic control), 200 mg/kg B.W of TW or RB or 100 mg/kg B.W of oxyresveratrol (OXY). Results: OXY and mulberroside A were identified as the major components of TW and OXY, mongolicin, and kuwanon H for RB. A significant inhibitory activity on ${\alpha}-glucosidase$ was found for TW, RB, and OXY (p = 0.0099). There was a dose-dependent inhibition of TW and RB on the intestinal sugar uptakes in Caco-2 cells, showing a greater impact on fructose compared to glucose. The OSTT showed that TW and RB significantly delayed time to maximal concentration (p = 0.0088) and decreased maximal concentration (p = 0.0043) compared to the control group. Conclusion: These results suggest that TW and RB may have a postprandial hypoglycemic effect, particularly in the case of high fructose or sucrose intake. OXY was suggested as a contributor to the hypoglycemic effect of TW and RB. Further studies are needed for the systemic effect of TW and RB in circulation.

Evaluation of Potential of Mandarin Hybrid 'Shiranuhi' against inoculation of Bacterial Canker Disease Pathogen (Xanthomonas axonopodis pv. citri) in Citrus Field in Jeju Island

  • Hyun, Jae-Wook;Myung, Inn-Shik;Lee, Seong-Chan;Kim, Kwang-Sik;Lim, Han-Cheol
    • The Plant Pathology Journal
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    • v.19 no.5
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    • pp.248-252
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    • 2003
  • This study was carried out to evaluate potential of Shiranuhi cultivar against inoculation of causal pathogen of citrus canker, Xanthomonas axonopodis pv. citri in Jeju Island by comparing degree of susceptibility of fruits and leaves/twigs, and analysis of incidence of canker disease. Progression of symptom, disease incidence, and percent area of lesion were surveyed for evaluation of resistance. In greenhouse condition, symptoms of bacterial citrus canker progressed more rapidly in sweet orange, a moderately susceptible cultivar, than in other four cultivars (satsuma mandarin, 'Kiyomi', 'Shiranuhi' and 'Yuzu'). At 20 days after inoculation, disease severity was the highest in sweet orange (5.0$\pm$0.0), and all tested leaves were distorted or had dropped. On the other hand, 'Yuzu' showed the lowest disease severity (2.6$\pm$0.47), followed by 'Kiyomi' (4.0$\pm$0.0), 'Shiranuhi' (4.0$\pm$0.82), and satsuma mandarin (4.3$\pm$0.47). Percent area of lesion per leaf 30 days after inoculation was the highest in sweet orange (8.31$\pm$1.78), followed by satsuma mandarin (1.51$\pm$1.25), 'Shiranuhi' (1.39$\pm$0.94), and 'Kiyomi' (1.1$\pm$0.9), while the lowest was in 'Yuzu' (0.26$\pm$0.17). Infield condition, percentage of diseased leaf in 'Shiranuhi' was very low, 5.2$\pm$2.9, compared with sweet orange, 71.0$\pm$ 11.5, while that of satsuma mandarin and 'Kiyomi' were 6.9$\pm$7.0 and 4.3$\pm$2.0, respectively. Percentages of diseased leaf was higher (17.4$\pm$7.1) than that of diseased fruit (3.2$\pm$2.5) in severely diseased trees of Shiranuhi cultivar, and the disease was not observed on twig in open field condition. Lesion sizes on leaves and fruits in open field condition were 4.1$\pm$2.2 mm2 and 5.1$\pm$5.6 mm2, respectively, while those in greenhouse condition were 8.7$\pm$5.7 mm2, 10.4$\pm$9.2 mm2 and 5.6$\pm$2.6 mm2 on leaves, fruits and twigs, respectively. The disease was observed in 5.6% out of total 107 farmers Shiranuhi fields under polyethylene film house, and average percentages of diseased tree in 31 fields of Shiranuhi cultivar and adjacent satsuma mandarin fields were 0.02% and 14.8%, respectively. Average percentage of diseased fruit was 1.6% in satsuma mandarin which was not observed in anyone of all the 31 Shiranuhi farmers fields. Therefore, it was concluded that 'Shiranuhi' cultivar is not potential against causal pathogen of citrus canker disease in Jeju Island because the cultivar has similar resistance as satsuma mandarin which occupies over 95% of total 25,000 ha in Jeju Island in polyethylene film houses protected from outside.

Effects of the Aquatic Vascular Plants on the Lake Ecosystem in the Upper Stream Wetlands of the Namgang-Dam (남강댐 상류 습지에서 수생관속식물이 호소생태계에 미치는 영향)

  • Oh, Kyung-hwan;Lee, Jeong-Hwan;Kim, Cheol-Soo;Son, Sung-Gon;Lee, Pal-Hong
    • Journal of Wetlands Research
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    • v.1 no.1
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    • pp.29-44
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    • 1999
  • Vegetation structure and distribution of the vascular hydrophytes and hygrophytes, and the growth pattern, standing crop and amounts of nutrient uptake by Salix species were investigated in the upper stream wetlands of the Namgang-Dam, Chinju-city, Gyeongsangnam-do, Korea from April to November in 1997. The flora was composed of 43 hydrophytes and 241 hygrophytes, or total 284 vascular plants. The life forms of the hydrophytes were classified as 27 kinds of emergent plants, 4 floating-leaved plants, 3 free-floating plants, and 9 submersed plants. In the herb layer, the dominant species was Persicaria hyciropiper, and the ranges of the species diversity indices (H'), equitabilities, (J') and community similarity indices (CCs) were 1.59~1.89, 0.87~0.96, and 0.35~0.83, respectively. In the shrub and subtree layers, 17 kinds of Salix species were supposed to the pioneer plants at the early stage of the succession. The number of branches per main stem of Salix species was 5.0. The DBH class-frequency histograms of Salix species were the reverse J type, and the natural regeneration of the Salix community was expected. Basal area of Salix species per square meter was $24.87cm^2$. Volume of Salix species per square meter was $12,008cm^3$ and total phytomass of the Salix species was estimated as 12,894 ton. Biomass distribution of Salix species in the stem, the branch and twig, and the leaf was 64.1%, 28.1%, and 7.8%, respectively. The amounts of nitrogen and phosphorus absorbed by Salix species were 68,022 and 19,823 kg. It was recommended that application and conservation of the wetland and other counterplans are indispensable to reduce the adverse effects of water pollution and to preserve the wetland ecosystem.

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Effects of Basic Oxygen Furnace Slag Application on Soils and Plant Growth at a Nursery and Pine Forests (제강(製鋼)슬래그의 시용(施用)이 묘포(苗圃) 및 소나무림의 토양(土壤)과 식물생장(植物生長)에 미치는 영향(影響))

  • Park, In Hyeop;Seo, Young Kwon;Lim, June Taeg;Lee, Choong Il
    • Journal of Korean Society of Forest Science
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    • v.90 no.6
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    • pp.699-706
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    • 2001
  • This study was conducted to evaluate the effectiveness of basic oxygen furnace (BOF) slag as soil conditioner in a nursery and two natural forests. The BOF slag was applied at rates of 0, 4, 8, and 12 t/ha for seedbed nursery of three tree species-Pinus densiflora, Larix leptolepis and Amorpha fruticosa; and 0, 10, 20, and 40 t/ha for Pinus densiflora natural forests located at two sites. In case of the nursery study, the significant increase in soil pH and contents of Ca, Mg, $SiO_2$, Fe and Mn was noticed after six months of the BOF slag application. Dry weight of Pinus densiflora seedlings with BOF slag treatments was lower compared with that of control. There were no significant differences among treatments in dry weight of Larix leptolepis seedlings. Dry weight of Amorpha fruticosa seedlings, which are deciduous species and grow rapidly at seedling stage, increased significantly and T/R ratio decreased as BOF slag rate increased. The differences of effects of BOF slag application on the seedlings of tree species may be attributed to the differences of optimum pH range of the species. In Pinus densiflora natural forests, there were no significant differences in soil pH and contents of $SiO_2$, Fe and Mn among the treatments, but these values became higher as BOF slag rate increased. Contents of Ca in soil became significantly higher as BOF slag rate increased. There were no significant differences among treatments in net production of mature Pinus densiflora trees, but BOF slag rate of 10ton/ha showed the highest net production. Young Pinus densiflora trees at the plots of BOF slag rate of 10 t/ha showed significantly higher root collar diameter growth rate and twig net production than those of other treatments. It may be given as a conclusion that BOF slag application in nursery and forest soil increased soil pH and contents of Ca, $SiO_2$, Fe and Mn in soil and they showed the ability of BOF slag to be used as a soil conditioner in strongly acid soil.

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Seasonal Development and Control of Parthenolecanium corni in Blueberry Shrubs (블루베리에 발생하는 말채나무공깍지벌레의 계절적 발육과 방제)

  • Lee, Seok-Min;Chung, Bu-Keun;Kang, Dong-Wan;Park, Kyung-Mi;Han, In-Young;Kwon, Jin-Hyeuk;Lee, Heung-Su
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.403-415
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    • 2021
  • Seasonal development of Parthenolecanium corni was observed from 4 Jun. 2019 (nymph) - 25 Jun. 2020 (1st Gen. nymph) in blueberry shrub in Sacheon city, Gyeongsangnam-do. To investigate their development, more than 5 twigs sprouted in 2018 were taken from the farm nearly weekly basis. The development of each scale were examined under the stereomicroscope and chemical control was conducted in the blueberry shrub with available three insecticides. The results on the development period and Centigrade Degree-Days accumulation (DDC) obtained are as follows: egg-laying period (peak): 12 -26 May 2020 (24 May)(DDC, 110.0-188.5 (173.6)); egg-hatching period (peak): 9 - 23 June 2020 (19 June)(DDC, 325.2-480.8(435.6); egg period: 26 days; nymph movement from overwintered adult to new leaves 16-25 June 2020 (DDC, 410,5-500.4); nymph movement from leaf to twig (peak) to become adult: 4-18 Feb. 2020 (8 Feb.). Eggs no. /adult (range): 956.8 ± 73.4 (13 - 3497). Size (mm) of egg, 0.29 ± 0.020(L), 0.15 ± 0.013(W); of egg-hatched nymph, 0.35 ± 0.018(L), 0.18 ± 0.007(W), 0.09 ± 0.007(eye distance); and of adult, 4.30 ± 0.893(L), 2.64 ± 0.520(W). The egg-hatched nymphs from the overwintered adult moved to the backside leaf of new shoot in which they found about 95% until leaf is falling by early February in next year. They overwintered as 2nd instar and occurred univoltine. For the control of the 1st instar crawler, three insecticides treated on 16 and 30 July at the registered dose for Ceroplastes japonicus. Acetamiprid 8WP showed 96.9% mortality at 21 days after 1st treatment.