• Microbiology and Biotechnology Letters
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• v.8 no.3
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• pp.173-180
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• 1980

A source of D-xylose was required for the enhanced production of D-glucose isomerase of Streptomyces sp. strain K-17. D-glucose supported the luxuriant growth of the organism as well as D-xylose, but D-glucose isomerase activity was hardly detected in the D-glucose-grown cells. When the D-glucose-grown cells were incubated aerobically for a few hours in 0.5% xylose solution in 0.05 M phosphate buffer, pH 7.0, it was found that inductive formation of D-glucose isomerase occurred in the cells without multiplication. In the non-growth phase of cells the inductive formation of D-glucose isomerase occurred because a source of nitrogen for the synthesis of enzymes was obtained from turnover of protein accumulated in cells. D-ribose, L-arabinose, D-glucose, D-mannose, citrate, succinate and tartrate could not induce the formation of D-glucose isomerase, but D-xylose could induce. Inductinn of D-glucose isomerase was repressed by D-glucose and its catabolites : glycerol, succinate and citrate. Inductive formation of the enzymes in the non-growth phase was stimulated by $Ba^{2+}$, $Mg^{2+}$ and $Co^{2+}$, and inhibited by C $u^{2+}$, C $d^{2+}$, A $g^{+}$and H $g^{2+}$. The synthesis of enzymes in the induction system composed of 0.5% xylose solution was disrupted by actinomycin D, streptomycin, chloramphenicol, kanamycin, tetracycline, p-chloromercuribenzo ate, arsenate and 2, 4-dinitrophenol, but not disrupted by mitomycin C and penicillin G.icillin G.

• Proceedings of the Microbiological Society of Korea Conference
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• 2004.05a
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• pp.141-146
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• 2004

SPIN90 was identified to farm molecular complex with $\betaPIX$, WASP and Nck. This complex shows that SPIN90 interacts with Nck in a manner dependent upon cell adhesion to extracellular matrix, but $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex was stable even in suspended cells. This suggests that SPIN90 serves as an adaptor molecule to recruit other proteins to Nck at focal adhesions. SPIN90 was phosphorylated by ERK1, which was, itself, activated by cell adhesion and platelet-derived growth factor. Such phosphorylation of SPIN90 likely promotes the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex and Nck. It thus appears that the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex with Nck is crucial for stable cell adhesion and can be dynamically modulated by SPIN90 phosphorylation that is dependent on cell adhesion and ERX activation. SPIN90 directly binds syndapin I, syndapin isoform II-1 and II-s via its PRD region in vitro, in vivo and also associates with endocytosis core components such as clathrin and dynamin. In neuron and fibroblast, SPIN90 colocalizes with syndapins as puntate form, consistent with a role for SPIN90 in clathrin-mediated endocytosis pathway. Overexpression of SPIN90 N-term inhibits receptor-mediated endocytosis. Interestingly, SPIN90 PRD, binding interface of syndapin, significantly blocks internalization of transferrin, demonstrating SPIN90 involvement in endocytosis in vivo by interacting syndapin. Depletion of endogenous SPIN90 by introducing $\alpha-SPIN90$ also blocks receptor-mediated endocytosis. Actin polymerization could generate farce facilitating the pinch-out event in endocytosis, detach newly formed endocytic vesicle from the plasma membrane or push out them via the cytosol on actin tails. Here we found that SPIN90 localizes to high actin turn over cortical area, actin-membrane interface and membrane ruffle in PDGF treated cells. Overexpression of SPIN90 has an effect on cortical actin rearrangement as filopodia induction and it is mediated by the Arp2/3 complex at cell periphery. Consistent with a role in actin organization, CFP-SPIN90 present in actin comet tail generated by PIP5 $kinase\gamma$ overexpression. Therefore this study suggests that SPIN90 is functional linker between endocytosis and actin cytoskeleton.

• Journal of Preventive Medicine and Public Health
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• v.23 no.1 s.29
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• pp.11-21
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• 1990

This study refers to the problem of long-term inpatient flow in a general hospital. In this study, a queueing simulation model was developed for the two departments in the hospital with a homogeneous case mix and relatively many long-term inpatients in order to increase the turnover rate and hospital charges. Before the simulation n, the model was verified by the Kolmogorov-Smirnov test. The following results were generated by three alternative models of the special bed policies. 1. Alternative I : When long term inpatients were admitted to the wards belonging to departments A and B without transfer to other departments and special beds, the average turn-over rate decreased by 2-4% and the average hospital charges decreased by 70 million won. 2. Alternative II : When long-term inpatients were transferred to department C but the transfer of wards was determined by department C in order of clinical need, the average turnover rate increased by 4-13% but the average hospital charges decreased by 30 million won. This result was not greatly different from the present state. 3. Alternative III : When long-term inpatients were transferred to the special wards and department C simultaneously, the increase in the average turnover rate and hospital charges was equivalent to the increase of two beds in the special wards. When the special wards were allocated 16 beds, the average turnover rate of departments A and B increased by about 55% and 20% respectively. Also, the hospital charges increased by about 0.44 billion won. As a result, transfer to department C and the use of 16 beds in the special wards for long-term inpatients of departments A and B is expected to maximize the hospital revenue. However, as the above special bed policy can not increase the turnover rate above 60%, there is a need for a more comprehensive policy to further increase the rate. The development of an elaborate model should include the number of long-term inpatients in all clinical departments, the special wards system or an increase of hospital beds to handle admission needs, and the resources of the hospital by department. When the alternatives are evaluated, a cost-benefit analysis in addition to the turnover rate and the hospital charges should be considered.

• Cartoon and Animation Studies
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• s.21
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• pp.71-84
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• 2010

The current distribution structure in Korea film industry is in the multiplex chain. As a result, most small or medium-sized theaters have been excluded from the market, and all the multiplex chain have gradually been formed into the vertical integration structure. The strike by multiplex theaters in Chuncheon who did not screen for one month led to the problem of the vertical integration, which resulted in many effects. Particularly, it caused considerable effect on the cultural desire and instigated movie-goers to turn to illegal content. This study surveys the process in which Korean multiplex theaters in vertical integration structure took over the Korean film market, and consequently, small theaters collapsed, and the theaters in vertical integration structure kick out small theaters in horizontal integration structure, and conducts a survey on the effect of the one-month strike by Chuncheon CGV from September 27 to October 30, 2008 in Chuncheon where said processes had completed so as to determine the effect of and impact of the problem of the vertical integration, monopoly structure. When major consumers of the film industry, i.e., people in their 20s, see their access to newly released films limited due to closing of multiplex theaters, they feel the impulse to access illegal contents. If they miss viewing the newly released films, they prefer to view illegal contents or films on recording media rather than visiting theaters showing those films. They are also influenced by timing of film release. Specifically, methods of illegal access to newly released films, permanent accessibility to them via recording media, and timing of film release influence their viewing patterns.

• Journal of Animal Science and Technology
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• v.44 no.3
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• pp.341-350
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• 2002

Twenty four Hanwoo bulls(mean initial body weight 162$\pm$8.1kg) were employed to induce the regression equations on body weight gain and dry matter intake. The diets were fed in a 3${\times}$4 factorial arrangement; three roughage sources(rice straw, hay, corn silage) and four roughage ratios in ration(100, 60, 40, 20%). Bulls were alloted by incomplete turn over design with four replications. Daily body weight gain was greatest in bulls fed the corn silage as a roughage source and the 20% of roughage ratio, respectively. Bulls fed hay or corn silage only as feed kept in their body weight gain during the whole experimental period, however those fed rice straw only were in a negative body gain hereafter fatteningⅠperiod(350kg of body wt.). Dry matter intake per metabolizable body weight was decreased with increasing body weight. Dry matter intake was greatest in bulls fed the corn silage as a roughage source and the 20% of roughage ratio, respectively. The regression equations on daily body weight gain(DG, kg) and dry matter intake(DMI, kg/day) according to body weight(BW, kg) of Hanwoo bulls were derived as follows; DG = －0.842 + 17.5DMI/$BWkg^{0.75}$ DMI = 4.125 + 0.013BW + 1.075DG － 0.045NDF where, NDF is the percentage of neutral detergent fiber in ration.

• Journal of Distribution Science
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• v.11 no.12
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• pp.5-11
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• 2013

Purpose - The purpose of this study was to construct a turnover estimation model by investigating research by Park et al. (2006) on the market area of domestic distribution. The study investigated distribution by using a new tool for the turnover estimation technique. This study developed and discussed the turnover estimation technique of Park et al. (2006), applying it to a large-scale retailer in "D"city that was suitable for on-the-spot distribution. It constructed the new model in accordance with test procedures keeping to this retail business location, to apply its procedures to a specific situation and improve the turn over estimation process. Further, it investigated the analysis and procedures of existing turnover estimation cases to provide problems and alternatives for turnover estimation for a large-scale retailer in "D"city. Finally, it also discussed problems and scope for further research. Research design, data, and methodology - This study was conducted on the basis of "virtue" studies. In other words, it took into account the special quality of the structure of Korea's trade zones. The researcher sought to verify a sale estimate model for use in a distribution industry's location. The main purpose was to enable the sale estimate model (that is, the individual model's presentation) to be practically used in real situations in Korea by supplementing processes and variables. Results - The sale estimate model is constructed, first, by conducting a data survey of the general trading area. Second, staying within the city's census of company operating areas, the city's total consumption expenditure is derived by applying the large-scale store index. Third, the probability of shopping is investigated. Fourth, the scale of sales is estimated using the process of singularity. The correct details need to be verified for the model construction and the new model will need to be a distinct sale estimate model, with this being a special quality for business conditions. This will need to be a subsequent research task. Conclusions - The study investigated, tested, and supplemented the turnover estimation model of Park et al. (2006) in a market area in South Korea. Supplementation of some procedures and variables could provide a turnover estimation model in South Korea that would be an independent model. The turnover estimation model is applied, first, by undertaking an investigation of the market area. Second, a census of the intercity market area is carried out to estimate the total consumption of the specific city. Consumption is estimated by applying indexes of large-scale retailers. Third, an investigation is undertaken on the probability of shopping. Fourth, the scale of turnover is estimated. Further studies should investigate each department as well as direct and indirect variables. The turnover estimation model should be tested to construct new models depending on the type of region and business. In-depth and careful discussion by researchers is also needed. An upgraded turnover estimation model could be developed for Korea's on-the-spot distribution.

• Journal of Distribution Science
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• v.9 no.4
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• pp.29-42
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• 2011

With the many advantages of the internet, online shopping has become one of the fastest growing types of retail businesses. However, internet-based firms are much more firmly required to retain existing customers rather than secure new ones, and to make them revisit the site by strengthening trust and loyalty, thereby improving profits and outrivaling competitors. Commitment is an essential part of successful long-term relationships between buyers and sellers. Although commitments by both parties in an exchange can provide the foundation for the development of relational social norms, disproportionate commitments can lead to opportunism by the less committed partner. Moreover, flow, which is characterized by intense concentration and enjoyment, was found to be significantly linked with exploratory use behavior, which in turn was linked to the extent of computer use. The level of flow was, itself, determined by the individual's sense of being in control, and the level of challenge perceived in maneuvering a website. Website attractiveness goes hand in hand with the attractiveness of an internet shopping mall, and it can be conceptualized as the persuasive effectiveness of a message by the use of familiarity, favor, similarity, etc. It occurs when information receivers try to achieve self-satisfaction when they actually or emotionally identify themselves with an information source. This study investigates the relationship between the perceived system characteristics of an internet shopping mall and the loyalty of online consumers, and it examines how perceived website attractiveness and flow play mediating roles between the perceived system characteristics of an internet shopping mall and the affective commitment in the context of a clothes internet shopping mall. For these purposes, a structural model comprising several variables was developed. That model was tested with an analysis of moment structure (AMOS) using data from respondents who had purchased clothing through the internet during the past three months. In this model, the perceived system characteristics of an internet shopping mall, such as familiarity, reputation, uniqueness, positive emotions, self-efficacy, and interactivity, were proposed to affect the website's attractiveness and flow, and lead to a higher affective commitment over time. Thus, the perceived website attractiveness and flow were proposed as core mediating variables between perceived system characteristics and affective commitment. The results of a reliability test using Cronbach's Alpha, and a confirmatory factor analysis warranted using unidimensionality for the measures for each construct. In addition, the nomological validity of the measures was warranted from the results of a correlation analysis. The results of empirical analyses indicated that systematic attributes resulting in website attractiveness and user's characteristics, thereby triggering customers' flow, play a crucial role in inducing customers' affective commitment, and a user's characteristics are twice as important as systematic attributes in this study. Moreover, familiarity, reputation, and uniqueness all have a significant effect on website attractiveness, and the research showed that uniqueness took the first place, and that familiarity and reputation followed in order of magnitude. The fact that reputation was not the most important factor that affects the attractiveness of an internet shopping mall, with uniqueness or familiarity having a greater impact, suggests much deeper implications. Finally, positive emotion, self-efficacy, and interactivity all have a significant effect on customers' flow. In particular, the fact that positive emotion, compared to self-efficacy or interactivity, has much more impact on flow is very suggestive.

• Cartoon and Animation Studies
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• s.6
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• pp.7-22
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• 2002

Animation constitutes the core of the media industry, which in turn lies at the center of the cultural industry. It is considered one of the industries where South Korea has the competitive edge over other countries. With the pool of customers getting wider, the genre of animation has become more and more diverse, forming a great market for it. Aware of this trend, this study focused on animation as a part of the pop culture, and on providing corresponding various viewpoints for future cultural studies. This researcher measured the practicality and persuasiveness of this study through Shreck, a three-dimensional C.G. animation which is acclaimed for its success in dismantling the old grammar of animation movies that represent the anti-Disney ideas. This researcher felt it imperative to heed the unique language of Shreck, which contains discourses on various cultural ideologies such as paradoxical structure that pits entertainment that is shown through dismantling of the canon, feminism and antifeminism against each other. This study analyzed the entertaining element of the animation genre by means of the Semiotics of Keith Moxey, thereby attempting to establish a legitimate social status of the genre, whose artfulness has been depreciated in the art society. In chapter II, this researcher examines the chronological development of three-dimensional C.G. animation that has shown a rapid advancement. Chapter III defines the cultural ideology of Shreck by exploring basic theories and texts employed in analysis of art works. This study started with the assumption that defines, from the viewpoint of symbology, the animation text as an aggregate of discourses on entertainment, and competitive and paradoxical ideologies. Then, this researcher analyzed the text and the generation process of meanings in Shreck. Consequently, this study has come to the following conclusions: First, Shreck induces changes of concepts about the canon by means of distorting and reversing the existing animation movies, which seems to reflect in the contemporary tendency of seeking new interpretations of entertainment. Second, Shreck shows up the cognitive changes of our age as to feminism by competing feminism against antifeminism. Although Shreck serves as a venue of competition between the two opposing ideas, it stops short of brushing off women as outsiders in society. Rather, it represents the resistance to the male chauvinism existing in the structures of animation and culture. As shown in the text analysis, Shreck presents an advent of a new ideology critical of the previous animation films. In addition, it reflects in the struggle between the pro-feminism on the part of the viewers and the anti-feminism that lies in the social and culture structure. This study, however, is limited in its scope and selection of subject. First, although this researcher has stressed the importance of understanding the animation as part of the pop culture and conducting researches within the historic paradigm, this study fails to provide an in-depth insight in the impacts that the changes in the C.G. industry and the systematic conditions may have on the three-dimensional C.G. animation genre. Furthermore, this study runs the risk of being understood as pro-American due to its selection of Shreck as its research subject.

• Journal of Navigation and Port Research
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• v.35 no.1
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• pp.93-100
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• 2011

This dissertation analysed actuality and literatures aimed at optimizing added value of Busan Port. As far as the literatures are concerned, the limits and the direction to go of Busan Port have been reached through analysing and comparing Busan Port, Antwerp Port, and Rotterdam Port by their each function. While Rotterdam and Antwerp Port are each functioning as an integrated port disposing container freight, general freight and liquid cargo, the container of Busan port contributes over 88 percent of its cargo and the function of the others like bulk is scattered to Ulsan, Masan, and Jinjae Port. Hereby, Busan Port needs to develop its function so as not to duplicate its function with the others in Korea and add value of theirs each. As a result of the local experts about analysing actuality, it turn out that it is similar with the literatures. In macroscopic view, specialization mainly with container, general cargo disposal ability enforcement and etc. are the most important. In microscopic view, rear complex of container tier and the supply base of liquid energy.

• Journal of the Korean Society of School Health
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• v.16 no.1
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• pp.149-160
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• 2003

The purpose of this study was to analyze the effects after having the obesity education with obese elementary school children. The subjects were made up of 31 obese children out of U elementary school in B town as experimental group, and also were selected 34 children out of S elementary school under the same circumstance as control group. They ranged from the third to sixth grades with over 30% body fat ratio. The proceeding of obesity education consisted of three stage; stage for preparation, stage for practice, and stage for finish. The stages mentioned above were put into practice in turn for eleven weeks beginning on Oct. 8, 2001 through Dec. 22, 2001, while two stages only, stage for preparation and finish, were put into practice on the control group. The contents of the education for eleven weeks obesity regulation contained counseling and taking regular exercises once a week by professional gym teacher. In order to evaluate the effects of the education, physical characteristics, body composition, physical fitness, knowledge of obesity, dietry habit and attitude were measured. The obtained data were analyzed using SPSS program, the effects of pre and post obesity education in experimintal group were measured by paired-t test. The conclusions of this study were as follows : 1. Body fat ratio was significantly reduced after obesity education in experimintal group(p=.003). 2. The height with experimental group got taller remarkably(p=.000), but weight showed no signeficance after obesity education(p=.258). 3. Triceps and subscapular skinfold thickness showed remarkable decrese after obesity education(p=.000), percentage body fat and body fat amount got down remarkably after obesity education(p=.000). Percentage lean body mass and lean body mass amount got down remarkably after obesity education(p=.000). 4. Body flexibility, agility and muscle endurance graded up remarkably after obesity education(p=.009, p=.000, p=.000). 5. The grade in obesity knowledge got up remarkably after obesity education(p.000), but dietry habits and its life showed no significance in figures after obesity education(p=.335, p=.112). Through the results shown above, the obesity education caused body fat ratio, physique, body composition to grade up and physical fitness elevated, while dietry habits and its life showed no significance.