• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.111-122
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• 2009

Compound fertilizer contained slow release nitrogen was used at golf course management because of promoting turfgrass growth and decreasing fertilizing labor. But, it was hardly published about turf grass growth by its effect and form like isobutylidene diurea(IBDU) and methylene urea(MU) in golf course management of Korea. This study was conducted to investigate the effect of compound fertilizer contained slow release nitrogen such as IBDU and MU on creeping bentgrasss and the change in nitrogen in the root zone after application of fertilizers at AM turfgrass research institute with soil analysis, chlorophyll content index, leaf area index and dry weight during 5 months from July to december in 2007 year. Fertilizer treatments were designed as following; non-fertilizer(NF), straight fertilizer(CF), treatment 1(MU), treatment 2(IBDU 1), and treatment 3(IBDU 2). The every treatments were arranged a randomized complete block design with three replications. Results obtained were summarized as follows: As relative to time after N fertilizers application, ammonium and nitrate in soil were increased, but T-N was decrease. $NO_3$-N and T-N content of slow release fertilizer(SRF), the treatment MU, IBDU 1 and IBDU 2, in soil was higher than that of CF, and the change of ratio of available N and T-N by elapsed time after fertilizing SRFs and CF was higher about 5% in root zone of SF to l5days, but in root zone of SRF from 30days about $5{\sim}10%$. Compared with NF, the turf color index was increased 6.5%, 6.7%, 5.9% and 5.5%, respectively, the chlorophyll content 33.2%, 38.4%, 35.1% and 37.1%, the dry weight 76.2%, 77.7%, 69.5 and 72.3% and shoot number 28%, 52%, 56% and 51%. The chlorophyll content index of MD, IBDU 1 and IBDU 2 was increased $2.6{\sim}5.2%$ than that of CF, shoot number $18{\sim}22%$ but turf color index and dry weight was analogous to that of CF. These results showed that the SRF application was supplied nitrogen for a long time in root zone by increasing an available nitrogen, and so bring turf growth such as chlorophyll content and shoot number in creeping bentgrass.

• Journal of Plant Biotechnology
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• v.43 no.2
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• pp.213-222
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• 2016

This study aims to establish a system for the rapid discrimination of Zoysia species using metabolite fingerprinting of FT-IR spectroscopy combined with multivariate analysis. Whole cell extracts from leaves of 19 identified Zoysia japonica, 6 identified Zoysia sinica, and 38 different unidentified Zoysia species were subjected to Fourier transform infrared spectroscopy (FT-IR). PCA (principle component analysis) and PLS-DA (partial least square discriminant analysis) from FT-IR spectral data successfully divided the 25 identified turf grasses into two groups, representing good agreement with species identification using molecular markers. PC (principal component) loading values show that the $1,100{\sim}950cm^{-1}$ region of the FT-IR spectra are important for the discrimination of Zoysia species. A dendrogram based on hierarchical clustering analysis (HCA) from the PCA and PLS-DA data of turf grasses showed that turf grass samples were divided into Zoysia japonica and Zoysia sinica in a species-dependent manner. PCA and PLS-DA from FT-IR spectral data of Zoysia species identified and unidentified by molecular markers successfully divided the 49 turf grasses into Z. japonica and Z. sinica. In particular, PLS-DA and the HCA dendrogram could mostly discriminate the 47 Z. japonica grasses into two groups depending on their origins (mountainous areas and island area). Considering these results, we suggest that FT-IR fingerprinting combined with multivariate analysis could be applied to discriminate between Zoysia species as well as their geographical origins of various Zoysia species.

• Weed & Turfgrass Science
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• v.2 no.1
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• pp.62-69
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• 2013

The black cutworm, Agrotis ipsilon (Hufnagel), damages various cultivated crops and it can also be a serious pest of turfgrass, especially on golf courses. Essential oils have potential as alternative control agents for insect pests. Sixteen essential oils (anise, camphor, cinnamon, citronella, clove, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, thyme, turpentine and tea saponin) and paraffin oil were assessed in the laboratory, the green house and field trials for their efficacy against black cutworms in turf. Treatment of potted cores of perennial ryegrass turf with anise, cinnamon, neem, paraffin or turpentine reduced black cutworm damage in a greenhouse trial, and in a similar trial, applying neem oil at 4000, 2000 and 1000 ppm resulted in 100, 100 and 64% mortality, respectively, of black cutworms. Weight of survivors at the 1000 ppm rate was 5- fold less than weight of comparably-aged controls. Neem oil (2000 ppm) reduced growth of black cutworms feeding on treated clippings. A high rate of neem oil followed by irrigation (0.1 L of 20000 ppm neem oil with 0.9 L watering/$m^2$) was more effective than a lower concentration (1 L of 2000 ppm neem oil/$m^2$) against $2^{nd}$ and $3^{rd}$ instars in potted turf cores and field plots, respectively. However, not even the aforementioned higher rate effectively controlled $4^{th}$ instars in the field.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.100-105
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• 2011

This study was conducted to evaluate the effect of developed SCB(DSCB) produced by adding N, P and K to SCB liquid fertilizer on the growth of creeping bentgrass. Fertilizer treatments were designed as follows; non-fertilizer (NF), control (CF; chemical fertilizer), 100 DSCB (250 $ml{\cdot}m^{-2}$DSCB), 80DSCB (200 $ml{\cdot}m^{-2}$DSCB) and CF+SCB (CF+250 $ml{\cdot}m^{-2}$SCB). Every treatment was arranged in a randomized complete block design with three replications. In creeping bentgrass, turf color index, chlorophyll index, dry weight, shoot number and nutrient content were measured. The results were as follows; Chemical properties of soil was hardly affected by DSCB and SCB applications. Turf color index and chlorophyll index in DSCB and SCB treatment were increased by 2~3% and 14~19% than those in NF, respectively, and similar to those of CF treatment. As applied to DSCB and SCB, shoot number was increased by 7%, 21%, 36% in 100 DSCB, 80 DSCB and CF+SCB than NF, respectively, and by 19% in CF+SCB than in CF. Supplying DSCB and SCB increased dry weight of creeping bentgrass, compared to CF treatment. Compared with CF, nitrogen and P content in tissue was increased in CF+SCB and in 80DSCB, respectively. These results suggested that applications of DSCB and SCB promoted turf quality and growth of creeping bentgrass by enhancing N and P uptake and shoot number.

• Asian Journal of Turfgrass Science
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• v.21 no.1
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• pp.69-79
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• 2007

Incorporating herbicides application into a fertilization program has several benefits including saving time and reducing traffics on the lawn. Premixed products of fertilizers and herbicides are commonly known as Weed & Feed in the lawn-care industry. To compare Weed & Feed with separate applications of fertilizers and herbicides on a Kentucky bluegrass (Poa pratensis L.) lawn, a Weed & Feed 28-3-3, containing 0.64% 2,4-D, 0.31% MCPP, and 0.03% dicamba of active ingredients, was used in this study. The first application was in May, with the second in June or Sept. Herbicides in forms of 2,4-D (LV-4, 4EC), MCPP (4EC), and dicamba (Clarity, 4EC) were applied at rates equal to the amounts in Weed & Feed or at half of the rates. The dominant weed in both locations was common dandelion (Taraxacum officinale Weber.) in 2005 and 2004. A secondary weed was Canada thistle (Cirsium arvense (L.) Scop.) in 2004 and broadleaf plantain (Plantago major L.) in 2005. When applied in May and June, fertilizer plus full rate of herbicides treatment achieved 112.3 and 83.7 days of acceptable turf quality in 2004 and 2005, respectively. During the same period, Weed & Feed resulted in 58.7 and 24.3 days of acceptable turf quality, respectively. Our study showed that Weed & Feed was generally as effective in weed control as the same amount of fertilizer plus half rates of herbicides sprayed although results may vary due to the timing of application. Fertilizer plus full rates of herbicides provided the same or better results of weed control than Weed & Feed.

• Asian Journal of Turfgrass Science
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• v.25 no.2
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• pp.153-159
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• 2011

This study was carried out to know the insect fauna of turf grass area in forest. In Korea, graves and public cemetery in mountain areas are usually covered by turf grass which were mainly examined. There were 86 species belonging to 77 genera of 39 families in 11 orders. Among them, site 3 had highest occurrence (38%), second was site 2 (36%) and in the last place was site 1 (26%). Hemiptera revealed best species composition (29.1%) and came Coleoptera (19.8%), Orthoptera (15.1%), Lepidoptera (12.8%) and Odonata (5.8%) etc. The reason why Hemiptera and Orthoptera are mainly found in these areas is because they usually feed on short plants that grow in there. Taxonomic species composition, occurrence ratio by site and species and comparison analysis were provided.

• Weed & Turfgrass Science
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• v.2 no.4
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• pp.402-407
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• 2013

Bermudagrass (Cynodon dactylon.) is one of the most difficult weedy species to control in turfgrass because it's high tolerant to various environmental and management stresses. This experiment was performed to find the integrated weed management including cultural practices to suppress bermudagrass in Zoysiagrass matrella (L) Merr. As results, two sequential applications of Fluazifop-P-butyl 0.05 ml $m^{-2}$ + Triclopyr-TEA 0.5 ml $m^{-2}$ and Fenoxaprop-P-ethyl 0.1 ml $m^{-2}$ + Triclopyr-TEA 0.5 ml $m^{-2}$ applied on 20 days intervals were evaluated the primary option for bermudagrass suppression and turfgrass injury was acceptable in zoysiagrass. In both treatments, turf injury was observed during 30days after the first application and almost recovered at 40days. While Fenoxaprop-Pethyl 0.1 ml $m^{-2}$ + Triclopyr-TEA 0.5 ml $m^{-22}$ were lightly phytotoxic to zoysiagrass in chlorophyll content test, there was no growth inhibition of zoysiagrass. Verticut practice (4 mm depth) just before herbicides application where zoyisagrass is contaminated with bermudagrass was not helpful to reduce turf injury in this experiment. However, alone verticut management was utilized to decrease about 12-14% bermudagrass population. Thus the application of Fenoxaprop-P-ethyl 0.1 ml $m^{-2}$ + Triclopyr-TEA 0.5 ml $m^{-2}$ which are permitted for turfgrass after zoysiagrass is perfectly recovered from turf injury by verticut practice should be utilized for bermudagrass reduction in zoysiagrass.

• Weed & Turfgrass Science
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• v.3 no.1
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• pp.41-49
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• 2014

Four preparations of liquid turfgrass fertilizers were tested cool season turfgrasses during fall season. The preparations of PS-A, PS-B, PH-C and PH-D for commercial products were contained with essential nutrient elements, seaweed extract, amino acids, and humus substance. Growth and quality of creeping bentgrass (Agrostis palustris Huds cv. Penn-A1) and Kentucky bluegrass (Poa pratensis L.) mixture (Midnight 33%, Moonlight 33%, Prosperity 33%) were evaluated by normalized difference vegetation index (NDVI), root length and fresh weight, shoot density, turf color, and chlorophyll content. Three foliar sprays of 4 preparations with an interval of 7 days were made in the fall of 2013 in the sod production field at Hapchun, Korea. No significant difference among 3 preparations was found in NDVI of creeping bentgrass. However, PS-A was significantly increased NDVI of Kentucky bluegrass. Two to three applications of PS-A significantly increased chlorophyll content and turf color. Three foliar sprays of PS-A and PS-B were significantly increased the shoot density of Kentucky bluegrass on 20 days after final application. These results may indicate that the use of some preparation is beneficial in producing higher quality turfgrass sod with better color and chlorophyll content during fall season.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.56-61
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• 2010

This study was conducted to evaluate the effect of application of SCB liquid fertilizer produced after slurry composting and biofiltration (SCB) on turfgrass growth in golf course during 6 month period from May to October in 2008. The change of soil properties by applying CF and SCB was unaffected respective treatments. As compared with NF, turf color index of CF, S-1 and S-2 increased by 1.8%, 2.5%, and 3.4%, respectively and chlorophyll content by 13%, 19%, and 25%, respectively. Dry weight of S-1 and S-2 was increased by 15% and 26% than that of CF. As compared with CF, N uptake rate in S-1 and S-2 was increased by 21% and 37%, P uptake rate 57% and 28%, and K uptake rate 16% and 27%, respectively. S-2 showed the best effect for turf color index, chlorophyll content, dry weigh and nutrient uptake rate in creeping bentgrass. These results suggested that an mixed application of SCB and compound fertilizer was improved turf quality and growth of creeping bentgrass by increasing nutrient uptake rate of turfgrass.

• Weed & Turfgrass Science
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• v.1 no.4
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• pp.57-63
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• 2012

This study was carried our to examine the genetic relationship of 13 commercial turfgrass cultivars using Random Amplified Polymorphic DNA to provide genetic informations more efficient golf course management. Analysis of 56 random hexamer primers generated 13 to 54 polymorphic bands among the 13 cultivars with an average of 30.7 bands per primer. The results of cluster analysis based on RAPDs revealed that three major variety groups: Group I - 'Shadow II', 'Aurora Gold', 'Little Bighorn Blue', 'PennA-1', and 'PennA-4'; Group II - 'Midnight II', 'Prosperity', 'Moon light SLT', 'Bright star SLT', and 'Silver dollar'; and Group III - 'Olympic Gold', 'Silver Star', and 'Tar Heel II'. The genetic similarity coefficients among 13 turfgrass cultivars ranged from 0.039 to 1.0 with highest coefficient in Group III. Studies on morphological characters and the effective molecular markers such as sequence characterized amplified regions are further needed to identify relationships and genetic diversities within species and among species.