• Title/Summary/Keyword: Turbidity control

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Cryptosporidium Oocyst Detection in Water Samples: Floatation Technique Enhanced with Immunofluorescence Is as Effective as Immunomagnetic Separation Method

  • Koompapong, Khuanchai;Sutthikornchai, Chantira;Sukthana, Yowalark
    • Parasites, Hosts and Diseases
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    • v.47 no.4
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    • pp.353-357
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    • 2009
  • Cryptosporidium can cause gastrointestinal diseases worldwide, consequently posing public health problems and economic burden. Effective techniques for detecting contaminated oocysts in water are important to prevent and control the contamination. Immunomagnetic separation (IMS) method has been widely employed recently due to its efficiency, but, it is costly. Sucrose floatation technique is generally used for separating organisms by using their different specific gravity. It is effective and cheap but time consuming as well as requiring highly skilled personnel. Water turbidity and parasite load in water sample are additional factors affecting to the recovery rate of those 2 methods. We compared the efficiency of IMS and sucrose floatation methods to recover the spiked Cryptosporidium oocysts in various turbidity water samples. Cryptosporidium oocysts concentration at 1, $10^1$, $10^2$, and $10^3$ per $10{\mu}l$ were spiked into 3 sets of 10 ml-water turbidity (5, 50, and 500 NTU). The recovery rate of the 2 methods was not different. Oocyst load at the concentration < $10^2$ per 10 ml yielded unreliable results. Water turbidity at 500 NTU decreased the recovery rate of both techniques. The combination of sucrose floatation and immunofluorescense assay techniques (SF-FA) showed higher recovery rate than IMS and immunofluorescense assay (IMS-FA). We used this SF-FA to detect Cryptosporidium and Giardia from the river water samples and found 9 and 19 out of 30 (30% and 63.3%) positive, respectively. Our results favored sucrose floatation technique enhanced with immunofluorescense assay for detecting contaminated protozoa in water samples in general laboratories and in the real practical setting.

Operation evaluation of DAF pilot plant for water treatment process in Hoedong Reservoir (회동수원지의 정수처리 공정을 위한 DAF pilot plant 운영 성능평가)

  • Maeng, Minsoo;Shahi, Nirmal Kumar;Kim, Donghyeun;Shin, Gwyam;Dockko, Seok
    • Journal of Korean Society of Water and Wastewater
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    • v.34 no.6
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    • pp.463-471
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    • 2020
  • A 1,000 ㎥/d DAF(dissolved air flotation) pilot plant was installed to evaluate the performance of the floating process using the Nakdong River. Efficiency of various DAF operations under different conditions, such as hydraulic loading rate, coagulant concentration was evaluated in the current research. The operation conditions were evaluated, based on the removal or turbidity, TOC(total organic carbon), THMFP(trihalomethane formation potential), Mn(manganese), and Al(aluminum). Also, particle size analysis of treated water by DAF was performed to examine the characteristics of particles existing in the treated water. The turbidity removal was higher than 90%, and it could be operated at 0.5 NTU or less, which is suitable for the drinking water quality standard. Turbidity, TOC, and THMFP resulted in stable water quality when replacing the coagulant from alum to PAC(poly aluminum chloride). A 100% removal of Chl-a was recorded during the summer period of the DAF operations. Mn removal was not as effective as where the removal did not satisfy the water quality standards for the majority of the operation period. Hydraulic loading of 10 m/h, and coagulant concentrations of 40 mg/L was determined to be the optimal operating conditions for turbidity and TOC removal. When the coagulant concentration increases, the Al concentration of the DAF treated water also increases, so coagulant injection control is required according to the raw water quality. Particle size distribution results indicated that particles larger than 25 ㎛ showed higher removal rates than smaller particles. The total particel count in the treated water was 2,214.7 counts/ml under the operation conditions of 10 m/h of hydraulic loading rate and coagulant concentrations of 60 mg/L.

Influences of Forest Environmental Factors on Turbidity of Stream Water (산림환경인자가 계류수의 탁수화에 미치는 영향)

  • Ma, Ho-Seop;Kang, Won-Seok;Kang, Eun-Min
    • Journal of Korean Society of Forest Science
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    • v.101 no.4
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    • pp.574-578
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    • 2012
  • This study was carried out to clarify the influences of forest environmental factors on turbidity of Stream water on three stands (Castanea crenata, Pinus densiflora and Plantation Land) of small watershed in Samgyeri Naedong-myeon Jinju-si Gyeongsangnam-do. The relationship between turbidity and forest environmental factors was a positive correlation at 1% level with chromaticity, suspended solid, sediment runoff erosion, slope, rainfall intensity, preceding dry days, watershed area and stream length and at 5% level with accumulative rainfall. The important factors that affected turbidity in small watershed showed in order of preceding dry days, rainfall intensity, stream length, chromaticity and suspended solid. In the stepwise regression between turbidity and forest environmental factors, the estimation equation is as follow; Y=-28.125+0.047x (suspended solid)+0.058x (chromaticity)+1.518x (rainfall intensity)+0.264x (stream length)+1.837x (preceding dry days). The results indicates that dangerous areas of landslide and soil runoff by land use could be applied to the mitigation measures such as afforestation, erosion check dam and revetment for erosion control and water quality management in small watershed.

Physicochemical and Sensory Characteristics of Dongchimi Added with Soybean-Curd Whey (두부순물 첨가 동치미의 이화학적.관능적 특성)

  • 김미리;김민정;백종연
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.6
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    • pp.1068-1075
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    • 2001
  • Effect of soybean-curd whey on the dongchimi fermentation was investigated by measuring physicochemical and sensory characteristics during fermentation at 1$0^{\circ}C$. Dongchimi was prepared with various levels (0, 2, 5, and 10%) of soybean-curd whey. During fermentation, whereas salt concentrations of dongchimi liquid (1.1 ~ 2.0%) were similar among treatments, greater decrease in pH, but greater increase of total acidity, reducing sugar content, number of lactic acid bacteria and turbidity were observed in dongchimi with soybean-curd whey than those of control from the 2nd to the 9th day of fermentation. But at the 16th day of fermentation, acidity, turbidity and number of lactic acid bacteria of dongchimi with soybean-curd whey were similar to those of control. Reducing sugar content of soybean-curd whey addition groups maintained higher than that of control. The Hunter color L value decreased gradually and that of dongchimi with soybean-curd whey was lower than that of control. b value of control increased, but soybean-curd whey addition groups decreased during fermentation. Hardness and fracturability, determined by texture analyser, were higher in soybean-curd whey addition groups than control. Sensory evaluation showed that the scores of turbidity, sweet taste, savory taste, hardness and over-all preference were significantly higher in soybean-curd whey addition groups than control (p<0.05) ; the highest with 5% soybean-curd whey addition followed by 2% addition. dongchimi with 5% soybean-curd whey addition was the most preferable one with the score of 6.5.

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Water Quality Analysis of In-stream and Reservoir Water in Erosion Control Dams in the Nakdong River Basin (낙동강유역 불투과형 사방댐의 계류수 및 저류수 수질 특성 분석)

  • Li, Qiwen;Eu, Song;Lee, Eun Jai;Lee, Ye Eun;Kim, Min Sik;Im, Sang Jun
    • Journal of Korean Society of Forest Science
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    • v.108 no.3
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    • pp.329-340
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    • 2019
  • Water pollution in erosion control dams is a major issue for forest watershed management, but the effects of erosion control dams on water quality remain poorly understood. In this study, water quality data from streams and dam reservoirs were collected over the Nakdong river basin. Monitored data were further analyzed to examine the relationship between watershed characteristics and water quality. Of the total 43 erosion control dams, less than 10% had lower water quality that was not suitable for agricultural use. TOC and oxygen demand, SS and turbidity, SS and Chl-a, and Chl-a and turbidity showed high correlations (p < 0.01) both in stream water and in detained water. BOD and SS, BOD and Chl-a, BOD and turbidity, and TOC and Chl-a showed high correlations only in stream water. Overall, the results demonstrated that for most erosion control dams the water quality was relatively good. In addition, the quality of downstream water is somewhat affected by the water detained by erosion control dams, in limited ways.

Distribution of Pathogenic Vibrios in the Aquatic Environment Adjacent to Coastal Areas of South Korea and Analysis of the Environmental Factors Affecting Their Occurrence (2016년도 국내 해양환경내 병원성 비브리오균의 분포 및 해양환경인자간의 상관성 분석)

  • Jeong, Young-Il;Myung, Go-Eun;Choi, Eun-Jin;Soh, Sang-Moon;Park, Gi-Jun;Son, Tae-Jong
    • Journal of Environmental Health Sciences
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    • v.44 no.2
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    • pp.133-142
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    • 2018
  • Objectives: The pathogenic Vibrios genus denotes halophilic bacteria that are distributed in aquatic environments, including both sea and freshwater. Vibrio cholerae, Vibrio vulnificus, and Vibrio parahaemolyticus are the most important species since they can be potent human pathogens and leading causes of septicemia, wound infections, and seafood borne gastroenteritis. The recent emergence of a potential pandemic clone, V. cholera serotype O1 and the cholera outbreak in South Korea in 2016 indicates the importance of consistent surveillance of pathogenic Vibrio genus within coastal areas. Methods: The present study was undertaken to determine where and how vibrios live in the aquatic environment adjacent to coastal areas of South Korea. For this survey, a total of 838 samples were obtained at 35 different sites in South Korean coastal areas during the period from January 2016 to December 2016. Pathogenic vibrios was determined using the real-time PCR method, and its clones were isolated using three selective plating media. We also monitored changes in seawater and atmospheric temperature, salinity, turbidity, and hydrogen ion concentration at the collection points. Results: The total isolation rates of V. vulnificus, V. cholera (non-pathogenic, non-O1, non-O139 serogroups), and V. parahaemolyticus from seawater specimens in 2016 were 14.2, 13.48, and 67.06%, respectively. Conclusions: The isolation rates of pathogenic vibrios genus showed a positive correlation with temperature of seawater and atmosphere but were negatively correlated with salinity and turbidity.

Optimization of Wet Noodle with Onion Juice using Response Surface Methodology (양파즙 첨가 생면의 최적화)

  • Shin, Weon-Sun;Shin, Eun-Soo;Lyu, Eun-Soon
    • Korean journal of food and cookery science
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    • v.25 no.1
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    • pp.31-38
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    • 2009
  • The noodle is one of the most popular meal as a main dish in Korea and onions are reported to contain high levels of flavonoids such as quercetin, aglycone, and quercetin 4-glycoside. This study was conducted to develop the wet noodle with onion juice and to examine the cooking quality, textural properties of it. Five sensory attributes such as gloss, chewiness, adhesiveness, odor, and taste were used for sensory evaluations. Optimal amount of onion(149.5 g) and salt (6.5 g) satisfied target sensory score(7.0) was suggested by response surface methodology. The wet noodle with optimal amount of onion juice and salt was tasted by 100 consumer and showed high score(6.67) in overall acceptability. Cooking quality was measured through absorption of water, volume, degree of turbidity. The volume, water absorption, and cooking water turbidity of cooked wet noodle with onion juice(experimental noodle) were significantly(p<0.01) higher than the control one. Texture profile analysis of experimental noodle showed a lower score of hardness, adhesiveness, springiness, and chewiness. However, it appeared that the experimental noodle showed higher score for cohesiveness than the control noodle.

A study on dissolved air flotation (DAF) process control using decision algorithm (의사결정 알고리즘을 이용한 DAF 공정 제어에 관한 연구)

  • Jung, Woosik;An, Ju-Suk;Park, Ji-Young;Oh, Hyun-Je
    • Journal of Korean Society of Water and Wastewater
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    • v.31 no.5
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    • pp.409-414
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    • 2017
  • In this study, we divided the process operation scenarios into three categories based on raw water temperature and turbidity. We will select and operate the process operation scenario according to the characteristics of the raw water. The number of algae in the DAF treated water has been analyzed to be less than 100 cells/mL. These results indicated that the DAF process is effective in removing the algae. In addition, the scenario of the integrated management decision algorithm of the DAF process was developed. DAF pilot plants ($500m^3/day$) process has shown a constantly sound performance for the treatment of raw water, yielding a significantly low level of turbidity (DAF treated water, 0.21~1.56 NTU).

Influence of Turbidity of Submerged Water on Photosynthetic Rate of Rice Plants (관수 탁도가 벼 광합성능에 미치는 영향)

  • 강양순;정근식;손양;김재철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.4
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    • pp.350-352
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    • 1988
  • This study was carried out to know the influence of turbidity of submerged water on photosynthetic rate of rice plant after water submerging treatment. Rice plants were transplanted in the pot at maximum tillering stage and they were sumberged for 3days at meiotic stage, 20days after transplanting, in the plastic container which had the clear and turbid water temperature adjusted around 24 to $25^{\circ}C$. Photosynthetic rate at 6 hours recovery after submergence was 41.5 to 54.2% compared to the control, but in the case of cultivar 'Nagdongbyeo', it was rapidly increased by 97.3 to 104.6% in the clear water and by 68.6 to 77.5% in the turbid water at 2 to 4 days recovery after submergence. Photosynthetic rates per plants at 6 hours to 4 days recovery after submergence were 128.3 to 245.5 $C_2$ mg.hr.$^{-1}$, in 'Samgamgbyeo' and 71.1 to 162.4 $CO_2$, mgㆍhr.$^{-1}$ in 'Nagdongbyeo' Photosynthetic rate to respiration ratio of rice plant recovery after submergence was lower compared to control plant and it was lower in 'Nagdongbyeo' than that in 'Samgangbyeo'.ngbyeo' than that in 'Samgangbyeo'.gangbyeo'.pos;.

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