• 대한한방내과학회지
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• 제12권2호
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• pp.96-112
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• 1991

Attempts were made to see the antitumor effects of Sigbunhwan widely used in Oh-jug(五積) employing tumor cells Lines such as K562 derived from erythroleukemia, Raji from lympoma and MO-4 from blastogenic tumor. Different concentrations of Sigbunhwan and combined therapy of Sigbunhwan and Bigihwan were treated to those tumor cells lines and then live cells were counted by Trypan blue assay and $^{3}H-Thymidine$ uptake assay. The results obtained were as follows. 1. $^{3}H-Thymidine$ uptake of various tumor cells lines when treated with high concentrations of Sigbunhwan for 48hours showed that the rate of DNA synthesis decreased 76% to 90% by the treatment of 1% Sigbunhwan but this inhibition was rather decreased when Sigbunhwan concentration was increased to 10, 15 and 20%.(Fig 3) 2. When Sigbunhwan was combined with Bigihwan which was also an antitumor drug, the effectiveness of tumor cells dealth was somewhat inceased showing a generally similar pattern to that of Bigihwan alone used.(Fig 4) This combination therapy also showed that higher concentrations of antitumor agent were no more·effective or rather harmful according to the tumor cells lines having different growth rate.(Fig 5,6) 3. The antitumor effects of combined Sigbunhwan and Bigihwan was decreased if the concentrations of this combination therapy was increased to 10 times showing relatively sluggish decrease in K562 and MO-4 but a sharp inhibitory effect in Raji which grows slowly.(Fig 7). 4. When Sigbunhwan was treated at low concentrations, K562 was more inhibited by 0.75% to 1.0% of Sigbunhwan while Raji was more inhibited by 0.25% to 0.5% of that.(Fig 8) 5. When Sigbunhwan was treated together with Bigihwan at low concentrations, the tumor cells death rate was 75% to 89% in Baji, 31% to 95% in MO-4 and 41 to 89% in K562, showing this combination therapy was more effect to Raji derived from lymphoma.(Fig 9) 6. The number of live tumor cells was correlated with optical density of MTT assay when measured with 2% Sigbunhwan treatment to tumor cells lines for 24 hours.(Fig 10) 7. 7 days treatment of 0.25% Sigbunhwan was compared with one day treatment of 1% suggesting long term treatment more effective.(Fig 11)

• Journal of Chest Surgery
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• 제31권4호
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• pp.362-368
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• 1998

편평세포폐암 환자 혈청내 SCC항원(squamous cell carcinoma antigen)의 암표지자로서의 유용성을 검정하고 암종이 성장함으로써 정상조직으로 침범하는 기전을 규명하기 위하여 폐암수술후 절제해 낸 폐암조직의 중심부와 말초부 그리고 암세포가 없는 정상 폐조직을 채취하여 SCC항원 농도와 DNA합성을 통해 세포성장과 분화에 관련이 있는 것으로 알려진 EGFr(epidermal growth factor receptor)의 농도를 측정하였다. 편평세포폐암종 조직내 SCC항원의 농도는 69＋25 ng/ml로 정상 폐조직 34＋7 ng/ml, 폐선암 35＋25 ng/ml보다 높았으며(p<0.05), EGFr의 농도는 폐암조직, 즉 편평세포암 47＋6 pmol/min, 선암 69＋20 pmol/min으로 정상 폐조직 34＋5 pmol/min, 39＋8 pmol/min보다 각각 높게는 나타났으나 유의성은 없었다. 암종의 크기에 따른 부위별 SCC항원의 농도는 암종이 직경이 3cm이하인 경우는 암종의 중심부(100＋82 ng/ml)가 말초부(55＋24 ng/ml)보다 높게 나타났으나(p>0.05), 5cm이상인 경우는 암종의 말초부(324＋92 ng/ml)가 중심부(34＋18 ng/ml)보다 현저히 높았다.(p<0.05) 그러나 부위별 EGFr의 농도는 암종의 크기에 따라 차이가 없었다. 암병기에 따른 부위별 SCC항원의 농도는 암중심부에서는 1, 2병기에서 3, 4병기로 암병기가 높아짐에 따라 감소하는 경향을 보였으나 통계적 유의성은 없었고(p>0.05), 암말초부에서는 1, 2병기 68＋37 ng/ml, 87＋35 ng/ml에서 3,4병기414＋87 ng/ml, 473＋226 ng/ml로 병기가 높아짐에 따라 현저하게 증가하였다.(p<0.05) 그러나 EGFr은 암중심부에서 1병기에서 2, 3, 4병기로 병기가 높아짐에 따라 증가하는 경향을 보였으나 통계적 유의성이 없었고(p<0.05), 암말초부에서는 병기에 따른 농도의 특이한 변화를 관찰할 수 없었다. 이상의 결과로 편평세포폐암종 조직내 SCC항원의 농도는 정상 폐조직이나 폐선암조직에서 보다 높게 나타나 혈청내 SCC항원의 농도가 편평세포폐암의 진단 및 치료효과를 예측하는데 유용한 암표지자로 생각되나, 암종내 부위별 SCC항원의 농도와 EGFr의 농도가 일치하지 않음으로써 암종이 성장함으로써 주위조직으로의 침범과 SCC항원의 농도와의 관계에 대해서는 보다 더 많은 연구가 필요할 것으로 사료된다.

• 대한세포병리학회지
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• 제8권1호
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• pp.62-68
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• 1997

Acinic cell carcinoma is a slow-growing solid neoplasm of salivary gland. Although their cytological and histological finding is bland-looking, their biological behavior is unpredictable. We experienced two cases of acinic cell carcinoma of the salivary gland diagnosed by fine needle aspiration biopsy and confirmed by tissue examination. They showed different clinical courses. We compared their cytologic and histologic findings. The first case was a right preauricular mass in a 58 year-old female of 3 years duration. The cytologic smear revealed sheets or small clusters of monotonous cells mimicking normal serous acinar cells with little cellular pleomorphism. She underwent superficial parotid lobectomy. The tumor was a well demarcated 1.5cm sized nodular mass without infiltration into surrounding parenchyme. The second case was a left submandibular mass in a 23 year-old male of 4 years duration. The smear showed more severe pleomorphism of the tumor cells than those of previous case. Excisional biopsy was done. The excised tumor was $5.5{\times}3.5{\times}3cm$ sized multilobulated solid mass with invasion into surrounding parenchyme. The tumor recurred after 20months, thus total excision of the mass and modified radical neck dissection was carried out. From the above findings, cytologic atypism, infiltrative growth pattern and type of initial therapy may be correlated with biologic behavior.

• Tuberculosis and Respiratory Diseases
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• 제43권3호
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• pp.440-448
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• 1996

Bronchial carcinoid tumors are uncommon, constituting approximately 3-5% of all primary lung cancers. Classification of these tumors has evolved substantially as our understanding of the cellular, biologic, and clinical aspects of these neoplasms has improved. Initially, bronchial carcinoids were thought to be benign and therefore were classified as bronchial adenomas. Currently, however, they are well recognized as having the potential for both local invasion and distant metastatic involvement. Consequently, carcinoid tumors are frankly malignant. Thus bronchial adenoma is a misnomer that should no longer be used for bronchial carcinoids. Most investigators currently favor classifying carcinoid tumors as a type of neuroendocrine neoplasm because of their potential to secrete a variety of chemical substances found in both the central nervous system and the epithelial cells of numerous organs. Bronchial carcinoids are usually characterized by a slow growth pattern and a low incidence of metastasis, and histologically conformed by the azurophil staining and the presence of the characteristic neurosecretary granule on electron microscopy. Atypical carcinoid tumor was first defined by Arrigoni et al, who proposed the following criteria for separation of atypical carcinoid from typical carcinoid tumor : 1) increased mitotic activity with 1 mitotic figure per 1-2 high power fields(or 5-10 mitoses /10 HPF), 2) nuclear pleomorphism, hyperchromatism, and an abnormal nuclear-cytoplasmic ratio, 3) areas of increased cellularity with disorganization of the architecture, and 4) tumor necrosis. In contrast, typical carcinoid tumor may have focal cytologic pleomorphism, but necrosis is absent and mitotic figures are rare. Recently we experienced a case of atypical bronchial carcinoid with multiple distant metastasis, so we report this case with a review of the literature.

• 응용통계연구
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• 제32권3호
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• pp.331-348
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• 2019

전이성 종양의 성장패턴 차이와 변화율에 따른 종양 이질성(tumor heterogeneity)을 파악하는 것은 종양세포의 약물에 대한 민감성을 파악하고 적절한 치료법을 찾아내기 위해 중요하다. 일반적으로 N개의 표본의 집단이 구분된다면 t-test 혹은 ANOVA 분석을 통해 집단별 평균의 차이에 대한 검정이 가능하다. 그러나 본 논문에서 다루는 데이터와 같이 집단이 구분되지 않는 경우 이러한 방법들은 사용될 수 없다. 표본들 사이의 이질성을 검정하기 위한 통계적 방법들이 연구되어 왔다. 최소 조합 t-검정 방법은 그 중 하나이다. 본 논문에서는 상이한 비율로 데이터를 양분하는 조합도 고려하는 최대 조합 t-검정 방법을 제안한다. 한편, 표본의 이질성을 검정하는 것이 군집분석에서 최적의 군집의 개수가 2개 이상인지를 검정하는 것과 같음에 착안하여 새로운 방법을 제안한다. 최대 조합 t-검정과 gap통계량을 이용하면 이전에 제안된 방법보다 개선된 제1종의 오류를 범할 확률과 검정력을 갖는다는 것을 모의실험을 통해 확인하였고 실제 자료 분석을 통해 결과를 도출하였다.

• Journal of Applied Biological Chemistry
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• 제54권1호
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• pp.15-20
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• 2011

홍삼은 우리나라에서 오랜 역사동안 여러 질병을 치료하는데 사용되었다. 본 연구에서는 홍삼 에탄올 추출물에 감마선을 1~5kGy 범위에서 조사를 하는 새로운 기법을 개발하고자 한다. 감마선 조사된 홍삼 추출물(IHRG)의 진세노사이드의 조성변화를 관찰하기 위해서 HPLC 분석을 이용하였다. 그 결과, 홍삼 에탄올 추출물에 감마선 1 kGy와 5 kGy를 조사한 처리군에서 진세노사이드의 조성에 변화는 관찰되지 않았다. MTT 분석법을 이용하여 사람의 전립선암세포주인 PC-3세포에서의 IHRG의 세포독성을 살펴 본 결과, 감마선을 처리하지 않은 홍삼추출액(HRG) 보다 더 높은 세포독성을 보였다. $LD_{50}$ 농도가 IHRG-1(1 kGy)에서는 $30{\mu}g/mL$, IHRG-5 (5 kGy)에서는 $15{\mu}g/mL$로 나타났다. 이러한 세포독성이 Annexin V/PI 분석 및 핵의 염색법인 DAPI 염색을 통하여 IHRG를 처리한 군들에서 전형적인 apoptosis를 관찰할 수 있었다. 또한, 산화적 스트레스(ROS)의 유발이 IHRG 처리군에서 나타났다. BALB/c 마우스에 암세포를 이식시킨 모델에서 IHRG에 의한 암세포 증식억제 효과를 살펴 본 결과, 암세포 증식 억제율이 HRG에서 21.1%인 반면에, IHRG-1에서 56.9%, IHRG-5에서 76.1%로 나타났다. 이들 결과들 통해, HRG에 어떤 생리활성 물질이나 성분들이 감마선 조사에 의해 항암효과를 증대시킨 것으로 사료된다.

• Journal of Chest Surgery
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• 제35권8호
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• pp.638-641
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• 2002

저등급의 섬유점액성 육종은 최근에야 진단적 분류가 이루어진, 드문 연부 조직 종양이다. 젊은 성인의 심부연부 조직에서 발생하는 경향이 있으며, 국소 재발이나 원격 전이가 가능하다. 진단적 범주가 확실히 정립되어 있지 않고 특정한 질환명으로 구분되고 있지 않다. 조직학적으로, 주로 소용돌이 양상으로 자라는 방추상세포와 점액성이나 섬유성 기질이 번갈아 관찰되는 것이 특징이다. 형태학적이나 면역조직학적 특징에 대한 세심한 고려가 상기 질환의 진단에 도움이 되며, 다른 양성이나 악성 연부 조직 종양과의 감별에 도움이 된다. 흉백에 발생한 저등급의 섬유점액성 육종을 경험하였기에, 문헌 고찰과 함께 보고하는 바이다.

• Journal of Chest Surgery
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• 제33권7호
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• pp.570-575
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• 2000

Background; Surgical resection remains the mainstay of treatment for esophageal cancer. Despite recent advances in surgical therapy, i.e. en bloc resection and extended lymphadenectomy, the overall long-term prognosis of patients with esophageal carcinoma has not, however, improved during the last decades. One of the major reasons in its relatively high recurrence rate. Material and Method; A retrospective review of recurrent patte군 of cancer in 42 patients who underwent curative surgery for primary esophageal cancer was performed clinically and histo-phthologically. Result; Nineteen patients had developed recurrece during the 18 to 52 months(mean 34.2 nonths), 8 had local recurrences, 1 had both, and 11 had systemic recurrences. Twelve patients(63%) had developed recurrence within 1 year, 5 patients(26%) between 1 year to 2 patients(11%) after 2 years. The recurrence rate according to growth pattern of tumor or presence of microinvasive findings was not statistically significant, but it increased significantly in clinical tumor stage III than stage IIA, B and in patients with the number of metastatic lymph node over ten. Conclusion; Post-operative recurrences of esophageal cancer appear as a high rate even though curative wide resection was done. Several clinical and histo-pathological factors correlate with the recurrence.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2013년도 춘계 종합학술대회 논문집
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• pp.361-362
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• 2013

As one of the steps toward understanding how the plant is well adapted to strongly saline habitats, the purple pigment compound that is accumulated in Suaeda japonica was extracted and characterized. The extracted pigment compound exhibited typical characteristics of betacyanin that were represented by water solubility, pH- and temperature-dependent color changes, sensitivity to light, UV-Vis spectra, and gel electrophoretic migration pattern. LC-MS of the extracted pigment compound showed the presence of two major protonated molecular ions ($[M+H]^+$) at m/z 651.1 and m/z 827.1. According to the DPPH assay, it was found to have an antioxidant activity that is linearly increased in proportion to the reaction time for up to 30 min, and the activity was comparable to that of control BHA at 9.0 mg/ml. The cytotoxic activity against several tumor cell lines was also examined following the MTT assay. The significant growth inhibitory effect was observed on two tumor cell lines, SW-156 (human kidney carcinoma) and HEC-1B (human endometrial adenocarcinoma). Probably, the pigment compound may function as an osmolyte to uphold halotolerant physiological processes in saline environment.

• 대한세포병리학회지
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• 제18권1호
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• pp.62-68
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• 2007

Invasive micropapillary carcinoma (IMPC) of the breast is recently described rare variant of invasive ductal carcinoma. This variant has a distinctive histological features and aggressive biological behavior. We reviewed the cytologic features of eight cases of IMPC. The cytologic smears showed moderate to high cellularity and the tumor tissue was composed of atypical, angulated, cohesive clusters of neoplastic cells with a papillary to tubuloalveolar architecture, and a morular growth pattern without fibrovascular cores was seen on the histopathology. IMPC of the breast has distinctive cytologic features and it is important to make an early diagnosis via fine needle aspiration cytology due to this tumor's aggressive behavior.