• The Korean Journal of Physiology and Pharmacology
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• v.22 no.6
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• pp.705-712
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• 2018

The tube formation assay is a widely used in vitro experiment model to evaluate angiogenic properties by measuring the formation of tubular structures from vascular endothelial cells (ECs). In vitro experimental results are crucial when considered the advisability of moving forward to in vivo studies. Thus, the additional attentions to the in vitro assay is necessary to improve the quality of the pre-clinical data, leading to better decision-making for successful drug discovery. In this study, we improved the tube formation assay system in three aspects. First, we used human endothelial colony forming cells (ECFCs), which are endothelial precursors that have a robust proliferative capacity and more defined angiogenic characteristics compared to mature ECs. Second, we utilized a real-time cell recorder to track the progression of tube formation for 48 hours. Third, to minimize analysis error due to the limited observation area, we used image-stitching software to increase the microscope field of view to a $2{\times}2$ stitched area from the $4{\times}$ object lens. Our advanced tube formation assay system successfully demonstrated the time-dependent dynamic progression of tube formation in the presence and absence of VEGF and FGF-2. Vatalanib, VEGF inhibitor, was tested by our assay system. Of note, $IC_{50}$ values of vatalanib was different at each observation time point. Collectively, these results indicate that our advanced tube formation assay system replicates the dynamic progression of tube formation in response to angiogenic modulators. Therefore, this new system provides a sensitive and versatile assay model for evaluating pro- or anti-angiogenic drugs.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.21 no.11
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• pp.1552-1561
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• 1997

In this numerical study, it is investigated for the ice-formation phenomena for water flow in a concentric tube. The freezing layers of ice in both the inner and outer wall of a concentric tube are simultaneously considered. In the solution strategy, the complete set of governing equations in both the solid and liquid regions are resolved. Numerical results are obtained by varying the inner/outer wall temperatures and Reynolds number. The results show that the inner/outer wall temperatures have the great effect on the thickness of the solidification layer thereof. The shapes of ice layer in both the inner and outer wall can be expressed as a function of inverse Graetz number. As the wall temperature in inner or outer tube decreases, the heat transfer coefficients in both inner and outer ice layer surfaces increase absolutely.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.10 no.1
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• pp.11-21
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• 1998

A numerical study is made on the ice-formation for water flow inside a stenotic tube. The study takes into account the interaction existing between the laminar flow and the stenotic port in the circular tube. In the solution strategy, the present study is substantially distinguished from the existing works In that the complete set of governing equations in both the solid and liquid regions are resolved. In a channel flow between parallel plates, the agreement of predictions and available experimental data is very good. Numerical results are mainly obtained by varying the height and length of a stenotic shape and additionally for several temperatures of the wall and inlet of tube. The results show that the shape of stenotic port has the great effect on the thickness of the solidification layer in the tube. As the height of a stenosis grows and the length of a stenosis decreases, the ice layer thickness near the stenotic port is thinner due to backward flow caused by the sudden expansion of water tunnel. It is also found that the ice layer becomes more fat In accordance with Reynolds number and the temperature of the wall and inlet of tube decreased.

• Journal of the Korean Wood Science and Technology
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• v.28 no.4
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• pp.10-16
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• 2000

Two wood decay ascomycetes fungi identified as Hypoxylon mammatum and H. truncatum were isolated from backyard of Korea Research Institute of Chemical Technology (KRICT) in Korea. Hypoxylon truncatum is newly recorded as a wood degrader in Korea. Unusual germination mechanisms of ascospores in H. mammatum and H. truncatum are described and illustrated. The differences between two species were noticed on the process of germ tube formation. In the process of germ tube formation, the fast movement to pigmented ascospores activated from their perispores was termed as spore eclosion that was only found in H. mammatum. This sophisticated recognition mechanism indicated the existence of specific eclosion and germ tube formation due to the composition of cell wall layers and their preferable host derive, based on examined two species under a genus. The observation on present study postulates different composition of wall layers of ascospore and different nutrient composition for germination.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.3
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• pp.357-366
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• 2003

An experimental study is performed to investigate characteristics of near of wakes of circular cylinders with serrated fins using a hot-wire anemometer for various freestream velocities. The main focus of this paper is to investigate a reason why a vortex formation length is increased suddenly. Velocity of the fluid which flow through fins decreases as fin's height and freestream velocity increases and fin pitch decreases, and a thickness of boundary layer increases. The finned tube has a lower velocity gradient when the higher boundary layer grows. This velocity gradient on finned tube makes a weak shear force in the wake and moves to downstream in a state of lower momentum transfer between the freestream and the wake. The phenomenon makes a vortex formation length increased suddenly. The fluctuations of the velocity distributions on the finned tube and (equation omitted) = 1.0 contour line in the vortex formation region decreases when the fin height increases and the pitch decreases.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2012.05a
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• pp.309-317
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• 2012

Recent years have witnessed the use of micro shock tube in various engineering applications like micro combustion, micro propulsion, particle delivery systems etc. The flow characteristics occurring in the micro shock tube shows a considerable deviation from that of well established conventional macro shock tube due to very low Reynolds number and high Knudsen number effects. Also the diaphragm rupture process, which is considered to be instantaneous process in many of the conventional shock tubes, will be crucial for micro shock tubes in determining the near diaphragm flow field and shock formation. In the present study, an axi-symmetric CFD method has been applied to simulate the micro shock tube, with Maxwell's slip velocity and temperature jump boundary conditions. The effects of finite diaphragm rupture process on the flow field and the shock formation was investigated, in detail. The results show that the shock strength attenuates rapidly as it propagates through micro shock tubes.

• Journal of Microbiology and Biotechnology
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• v.16 no.3
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• pp.475-479
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• 2006

Gentisyl alcohol isolated from Penicillium sp. has an antioxidative activity, protecting cells from oxidative stresses. From our in vitro angiogenesis assays with bovine aortic endothelial cells (BAECs), gentisyl alcohol was newly identified as a pro-angiogenic small molecule that induces new blood vessel formation of the cells. Gentisyl alcohol stimulated the proliferation of BAECs in a dose-dependent manner. Moreover, it induced in vitro angiogenesis of BAECs such as invasiveness, migration, and tube formation of the endothelial cells. Effects of gentisyl alcohol on invasion and tube formation were also dose-dependent. These results demonstrate that gentisyl alcohol could affect the angiogenic phenotypes of endothelial cells and be developed as a new small molecule with pro-angiogenic activity.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.11 no.3
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• pp.377-383
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• 1999

In this study, the behavior of frost formation according to surface characteristics in the fin-tube heat exchanger has been examined experimentally. The results show that the thickness of the frost which is attached to the hydrophilic heat exchanger becomes thin and the air pressure drop is smaller than that of bare aluminium heat exchanger However, the frost mass of hydrophilic heat exchanger is more than the bare one. Hence, high density frost is attached to hydrophilic heat exchanger. The sensible and latent heat flux of hydrophilic heat exchanger is bigger than that of bare one, but the increasing amount is very small and the improvement of thermal performance is also very small. The variation of fin-pitch of heat exchanger shows little influence on frost formation and hydrophilic heat exchanger loses its surface characteristics rapidly with increasing relative humidity.

• Toxicological Research
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• v.28 no.1
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• pp.33-38
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• 2012

In this study, we investigated the effect of methanolic extract isolated from the root of Lycoris aurea (LA) on the growth of cancer cells and the tube formation activity of endothelial cells. Various cancer cells were treated with LA at doses of 0.3, 1, 3, 10 or 30 ${\mu}g/ml$ and LA significantly suppressed the growth of several cancer cell lines, including ACHN, HCT-15, K-562, MCF-7, PC-3 and SK-OV-3, in a dose-dependent manner. We also found that LA induced cell cycle arrest at G2/M phase in ACHN renal cell adenocarcinoma cells. Further study demonstrated that LA concentration-dependently inhibited the tube formation, which is a widely used in vitro model of reorganization stage of angiogenesis, in human umbilical vein endothelial cells. Collectively, these results show that LA inhibits the growth of cancer cells and tube formation of endothelial cells and the growth-inhibitory effect of LA might be mediated, at least in part, by blocking cell cycle progression.

• Journal of the Korean Society of Propulsion Engineers
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• v.17 no.3
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• pp.37-46
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• 2013

Recent years have witnessed the use of micro shock tube in various engineering applications like micro combustion, micro propulsion, particle delivery systems etc. The flow characteristics occurring in the micro shock tube shows a considerable deviation from that of well established conventional macro shock tube due to very low Reynolds number and high Knudsen number effects. Also the diaphragm rupture process, which is considered to be instantaneous process in many of the conventional shock tubes, will be crucial for micro shock tubes in determining the near diaphragm flow field and shock formation. In the present study, an axi-symmetric CFD method has been applied to simulate the micro shock tube, with Maxwell's slip velocity and temperature jump boundary conditions. The effects of finite diaphragm rupture process on the flow field and the shock formation was investigated, in detail. The results show that the shock strength attenuates rapidly as it propagates through micro shock tubes.