• 현장농수산연구지
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• 제8권1호
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• pp.55-62
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• 2006

Tuber melanosporum(Perigord black truffle)와 T. aestivum(Burgundy truffle) 등 유럽의 트러플은 아주 고귀한 식품이며 세계적으로 kg당 수백 달러에 팔리고 있다. 이들 지하경 버섯은 미남중부의 산림과 공생하면서 공익을 끼친다. Tuber melanosporum(Perigord black truffle)와 T. aestivum(Burgundy truffle)은 뉴질랜드와 미국의 혼합기술결과로 제한된 범위에서 재배되어 왔다. 튜버종은 상호적으로 백색과 상록 참나무류와 헤즐럿의 기주체의 뿌리에서 외생균근으로 자란다. Tuber melanosporum(Perigord black truffle)와 T. aestivum(Burgundy truffle)은 미남중부 지역에서 환경적인 필요조건을 가지고 있다. T. melanosporum은 재배에 있어서 기후적 토양적 조건이 더욱 인위적인 조치를 많이 취하여 하므로 훨씬 고가이다.

• 현장농수산연구지
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• 제8권1호
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• pp.63-70
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• 2006

트러플은 적정한 살균배지에 포자 균이 자랄 수 있는 수종의 묘목이 자람에 따라 재배가 된다. 온실에서 접종된 묘목을 기르면서 균근관계가 형성되고 증명된다. 준비된 적정지역에 감염묘를 식재하여 지하덩이를 유도하고 트러피어를 조성해 나가야한다. 트러플 재배를 위한 가장 좋은 관리방법과 각 단계별 과정을 어떻게 해야 최선의 방법인지는 우리 모두의 숙제이다.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.28-28
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• 2007

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• Mycobiology
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• 제49권6호
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• pp.527-533
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• 2021

The truffle and ectomycorrhizal roots formed by Tuber sp. were collected from the rhizosphere of Quercus aliena in Korea. The morphological characteristics of the ascoma, and molecular phylogenetic analysis using sequences from the internal transcribed spacer (ITS) and large subunit (LSU) of ribosomal DNA, translation elongation factor 1-alpha (TEF), and RNA polymerase second largest subunit (RPB2) regions confirmed the distinct morphology of the truffle. This truffle belongs to a monophyletic clade among the other Tuber species in the phylogeny. This study describes the truffle, Tuber koreanum, as a new species reported from Korea.

• 한국미생물·생명공학회지
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• 제50권1호
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• pp.40-50
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• 2022

The Périgord black truffle (Tuber melanosporum) is an edible fungus and among the most expensive foods worldwide. It is the basis of a multimillion-dollar bio-business. Truffle farming does not exist in Mexico, and no formal studies have been conducted on its culture. This report describes the mycorrhizal synthesis (i.e., artificial union of fungus with host) of T. melanosporum with oak species native to Mexico (Quercus polymorpha, Q. fusiformis, and Q. canbyi). The mycorrhizal association was successful in Q. polymorpha and Q. fusiformis, as confirmed morphologically and using T. melanosporum molecular primers (ITSML/ITS4LNG). The effect of the ectomycorrhizal fungus on host growth (stem diameter) was statistically significant. Illustrations of the study are presented.

• Mycobiology
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• 제49권3호
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• pp.201-212
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• 2021

Truffles are the fruiting bodies of ascomycete fungi that form underground. Truffles are globally valued, culturally celebrated as aphrodisiacs, and highly sought-after delicacies in the culinary world. For centuries, naturalists have speculated about their mode of formation, and in cultures surrounding the Mediterranean Sea, many species have been prized as a delectable food source. Truffle fruiting bodies form underground and emit a variety of volatile organic compounds (VOCs). Truffle volatiles are believed to have evolved to attract animals that disperse their spores. The main VOCs identified from truffles include sulfur compounds, such as dimethyl sulfide (DMS) and dimethyl disulfide (DMDS); in addition, 1-octen-3-ol and 2-methyl-1-propanol have been found in most truffle species. Humans use pigs and dogs trained to detect truffle VOCs in order to find these prized subterranean macrofungi. Truffles have pharmacological potential, but until more reliable cultivation methods become available their high price means they are unlikely to see widespread use as medicinals.

• Mycobiology
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• 제46권1호
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• pp.64-71
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• 2018

The aim of this study was to investigate the pattern of distribution of mating type (MAT) genes of Tuber indicum in ectomycorhizosphere soils from natural T. indicum-producing areas and cultivated truffle orchards and ascocarp samples from different regions. Quantitative real-time PCR and multiplex PCR were used to weight the copy numbers of MAT1-1-1 and MAT1-2-1 in natural truffle soils and cultivated orchard soils. The effect of limestone on the pattern of truffle MAT genes and the correlation between soil properties and the proportion of MAT genes were also assessed. These results indicated that an uneven and nonrandom distribution of MAT genes was common in truffle-producing areas, cultivated truffle orchards, and ascocarps gleba. The competition between the two mating type genes and the expansion of unbalanced distribution was found to be closely related to truffle fructification. Limestone treatments failed to alter the proportion of the two mating type genes in the soil. The content of available phosphorus in soil was significantly correlated with the value of MAT1-1-1/MAT1-2-1 in cultivated and natural ectomycorhizosphere soils. The application of real-time quantitative PCR can provide reference for monitoring the dynamic changes of mating type genes in soil. This study investigates the distributional pattern of T. indicum MAT genes in the ectomycorhizosphere soil and ascocarp gleba from different regions, which may provide a foundation for the cultivation of T. indicum.

• Mycobiology
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• 제50권2호
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• pp.104-109
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• 2022

Fungi of the genus Tuber are ectomycorrhizal fungi that form a symbiotic relationship mainly with oak and hazel trees. Tuber spp. exhibit a highly selective host plant preference; thus, for cultivation purposes it is important to select an appropriate host plant for successful mycorrhization. In addition, as mycorrhizal characteristics differ according to Tuber spp., it is necessary to understand the differences in mycorrhizae according to the fungal species. Tuber huidongense and Tuber himalayense were recently discovered in Korea; therefore, we used spore suspensions from these two species to inoculate two species of oak trees, Quercus acutissima and Quercus dentata, to compare colonization rates and morphologies of the mycorrhizae. The colonization rates demonstrated that the different Tuber spp. favored different host plant species. In addition, unique morphological and anatomical characteristics were observed for T. huidongense and T. himalayense depending on the host species. These findings can lead to new economically important agricultural activities related to truffle cultivation in Korea.

• 한국균학회지
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• 제51권2호
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• pp.121-125
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• 2023

In this study, we examined the ectomycorrhiza (ECM) formed by a species of the genus Tuber, T. koreanum, with two indigenous Korean oak species, Quercus acutissima and Q. dentata. T. koreanum showed a high colonization rate for both oak species, with a higher mean colonization rate observed for Q. acutissima. The mycorrhizal characteristics of T. koreanum were described, revealing distinct morphological and anatomical differences between the ECM formed with the two host plants. This study is significant because it is the first to elucidate ECM formations of T. koreanum in indigenous oak trees. However, further studies are required to differentiate the ECM associations formed by T. koreanum from those of other Tuber species.

• Mycobiology
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• 제50권4호
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• pp.238-243
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• 2022

Members of the genus Tuber are ectomycorrhizal fungi; this genus includes more than 180 species worldwide. In the present study, the optimal pH, temperature, and medium suitable for the mycelial growth of the Korean truffle, Tuber koreanum, were determined. Mycelium of T. koreanum, isolated from fruiting bodies collected in Korea, was used to investigate the effects of these environmental factors. The results showed that malt extract agar and potato dextrose agar were the most suitable for the mycelial growth of T. koreanum when cultured at a pH of 6.0 at 25 ℃ for 30 days.