• Title/Summary/Keyword: Trscability

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Analysis and Selection of Microsatellites Markers for Individual Traceability System in Hanwoo (한우 생산이력제에 활용 가능한 Microsatellite의 분석과 선발)

  • Lim, H.T.;Min, H.S.;Moon, W.G.;Lee, J.B.;Kim, J.H.;Cho, I.C.;Lee, H.K.;Lee, Y.W.;Lee, J.G.;Jeon, J.T.
    • Journal of Animal Science and Technology
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    • v.47 no.4
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    • pp.491-500
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    • 2005
  • To test applicability to the Hanwoo traceability system, twenty microsatellite markers were selected and analyzed. MSA, CERVUS, FSTAT, GENEPOP, API_CALC and PHYLIP software was employed serially to estimate heterozygosity, polymorphic information content, F-statistics, identity probability, exclusion probability and genetic distance. Eleven microsatellite markers(TGLA53, TGLA227, ETH185, TGLA122, BM4305, INRA23, ILSTS013, BMS1747, BM2113, BL1009, and ETH3) were selected based on their high heterozygosity values. Identity probability using these markers is one hundred times higher than when using StockMakersTM of Applied Biosystems. This indicates the selected microsatellite markers are appropriate and effective for use in the Hanwoo traceability system. Additionally, estimates of DA genetic distance and pairwise-FST can be utilized to identify genetic relationships between adjacent farms.