• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.1
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• pp.64-71
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• 2011

Health beneficial properties of soybean [Glycine max (L.) Merr.] isoflavones are well known. The objectives of this study were to determine and compare the isoflavone composition and concentrations of soybean varieties grown in different cultivated regions of Vietnam (i.e., upland and lowland). Total and individual isoflavone composition and concentrations were determined by high-performance liquid chromatography (HPLC). Total isoflavone concentrations varied from 1153 to $6604{\mu}g\;g^{-1}$ and averaging $3354{\mu}g\;g^{-1}$ across environments and varieties. In the lowland region, the highest total isoflavones concentration was observed in M103 cultivar ($5653{\mu}g\;g^{-1}$) and the lowest in VX9-3 ($1153{\mu}g\;g^{-1}$), whereas in the upland region the highest and lowest concentrations were in M103 ($6604{\mu}g\;g^{-1}$) and DT93 ($1938{\mu}g\;g^{-1}$), respectively. Across varieties, average total isoflavones concentration was higher in the upland than lowland region (3728 vs. $2980{\mu}g\;g^{-1}$). The malonylglucosides and acetylglucosides concentrations in upland soybean varieties were higher than those from the lowland region. Despite the presence of Genotype (G) x Environment (E) interactions, varieties with consistently high (M103) and low (VX9-3, DT93) isoflavone concentrations across environments were identified. This is the first report of isoflavones in Vietnamese soybean varieties, revealing large variation in isoflavones concentration and profile among different varieties and cultivated regions. Results will be useful in selecting high-isoflavones soybean varieties for growth in tropical regions.

• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.124-129
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• 2015

We have previously characterized the complete fibroin light chain gene from one of the silkworm race Baegokjam (Bombyx mori) and found two variable regions (FibL1, intron 2 ~ 3; FibL2, intron 6) with the primer sets designed to cover these variable regions. In this study, we tested the utility of these variable regions as genetic markers for classifying silkworm races. For the purpose, Europian races (Q, PK), Chinese races (C26, C31), Japanese races (N15, N9), and tropical races (SA2, SA5) were used in this experiment. The polymorphism of the FibL1 and FibL2 were divided into two and three types, respectively. The combination of the FibL1 and FibL2 polymorphisms were constant within the same races. The result suggest that the primer sets designed from two variable regions of fibroin light chain gene may be useful as the genetic markers for silkworm races.

• Parasites, Hosts and Diseases
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• v.62 no.1
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• pp.64-74
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• 2024

This pilot study aimed to investigate the effects of regional and seasonal variations on the prevalence of Theileria orientalis and the hematological profile of non-grazed dairy cows in Korea. A total of 365 clinically healthy lactating Holstein Friesian cows from 26 dairy farms in 7 provinces that were categorized into northern, central, and southern regions were sampled during the warm period from July to August and the cold period from October to December. The detection of T. orientalis major piroplasm surface protein gene and the hematology non-grazed dairy cows were analyzed using peripheral blood samples. The T. orientalis prevalence was 20.0% (73/365). The prevalence in the southern region was 35.9%, which was significantly higher than that in the central (21.6%) and northern (12.9%) regions (P<0.05). The prevalence during warm period was higher (43.0%) than that during the cold season (13.5%). The infected cows showed significantly lower erythrocyte counts in the southern region (5.8±0.6 M/㎕) and during the warm period (5.8±0.7 M/㎕) compared with those in the central and northern regions and during the cold season, which affected the extended RBC parameters, including hematocrit and hemoglobin concentrations. Our findings revealed the prevalence of T. orientalis in Korea, highlighting its high occurrence during warm periods and in certain geographical regions. Climatic factors could contribute to the health and productivity of cattle, as evidenced by the prevalence of T. orientalis and its negative impact on animals.

• Journal of Environmental Science International
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• v.4 no.5
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• pp.28-28
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• 1995

The aim of the present study is to investigate the interannual variabilities of the East Asia monsoon rainfall associated with the global sea surface temperature anomaly(SSTA). For this study, the summer rainfall(from June to August) over the twenty-eight period of 1961-1988 were analyzed with being divided by nine-subregions over East Asia including Korea, China and Japan. From the analysis of the principal modes explaining the interannual variation, the interannual variabilities of summer rainfalls in South Japan and Korea are larger than those of the other subregions of the East Asia. There is a strong negative correlation between the summer rainfalls of south China and Korea. In this study, the relationship between the summer monsoon of each subregion and SSTs of the tropical NINO regions, of western Pacific warm pool, and of the subtropical ocean were investigated. The longitudinal sections of the lagged cross correlations of the summer rainfal1 anomaly in (a) Korea and (b) south China, and the monthly SSTA in the equatorial(averaged from 65 to 6N) Pacific were analyzed. The negative maximum correlation pattems of Korea''s stammer rainfal1 and SSTs over the eastern Pacific is transfered to positive maximum conrlation over central Pacific region with a biennial periodicity. In South China, the significant positive correlations are found at -12 month lag over the eastern Pacific and maximum negative correlation at 16 month lag over the central Pacific with the quasi-biennial oscillation. But the correlation coefficient reverses completely to that in Korea. In order to investigate the most prevailing interannual variability of rainfall related to the favored SSTA region, the lagged cross correlations between East Asia rainfall and SSTs over the moO regions(NINO 1+2(0-105, 90W-80W), NINO 3(5N-5S, 150W-90W), NINO 4(5N-5S, 160E-l50W) and the western Pacific worm pool (5N-5S, 120E-l60E) were analyzed. Among the lagged cross-correlation cycles in NINO regions, the maximum correlations for the negative lagged months prevail in NINO 1+2 and NINO 3, and the cross correlations for the positive lagged months NINO 4. It is noteworthy that correlation between the western Pacific warm pool SSTA and the monsoon rainfall in Korea and South China have the maximum value at negative 4 month lag. The evolution of the correlation between the East Asia monsoon rainfall and SSTA is linked to the equatorial convective cluster and related to northward propagating situation, and raising the possibility that the East Asia monsoon precipitation may be more fundamentally related to the interaction of intraseasonal oscillations and the sub-regional characteristics including the surface boundary conditions and the behavior of climatological air mass.

• Journal of Environmental Science International
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• v.4 no.5
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• pp.413-426
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• 1995

The aim of the present study is to investigate the interannual variabilities of the East Asia monsoon rainfall associated with the global sea surface temperature anomaly(SSTA). For this study, the summer rainfall(from June to August) over the twenty-eight period of 1961-1988 were analyzed with being divided by nine-subregions over East Asia including Korea, China and Japan. From the analysis of the principal modes explaining the interannual variation, the interannual variabilities of summer rainfalls in South Japan and Korea are larger than those of the other subregions of the East Asia. There is a strong negative correlation between the summer rainfalls of south China and Korea. In this study, the relationship between the summer monsoon of each subregion and SSTs of the tropical NINO regions, of western Pacific warm pool, and of the subtropical ocean were investigated. The longitudinal sections of the lagged cross correlations of the summer rainfal1 anomaly in (a) Korea and (b) south China, and the monthly SSTA in the equatorial(averaged from 65 to 6N) Pacific were analyzed. The negative maximum correlation pattems of Korea's stammer rainfal1 and SSTs over the eastern Pacific is transfered to positive maximum conrlation over central Pacific region with a biennial periodicity. In South China, the significant positive correlations are found at -12 month lag over the eastern Pacific and maximum negative correlation at 16 month lag over the central Pacific with the quasi-biennial oscillation. But the correlation coefficient reverses completely to that in Korea. In order to investigate the most prevailing interannual variability of rainfall related to the favored SSTA region, the lagged cross correlations between East Asia rainfall and SSTs over the moO regions(NINO 1+2(0-105, 90W-80W), NINO 3(5N-5S, 150W-90W), NINO 4(5N-5S, 160E-l50W) and the western Pacific worm pool (5N-5S, 120E-l60E) were analyzed. Among the lagged cross-correlation cycles in NINO regions, the maximum correlations for the negative lagged months prevail in NINO 1+2 and NINO 3, and the cross correlations for the positive lagged months NINO 4. It is noteworthy that correlation between the western Pacific warm pool SSTA and the monsoon rainfall in Korea and South China have the maximum value at negative 4 month lag. The evolution of the correlation between the East Asia monsoon rainfall and SSTA is linked to the equatorial convective cluster and related to northward propagating situation, and raising the possibility that the East Asia monsoon precipitation may be more fundamentally related to the interaction of intraseasonal oscillations and the sub-regional characteristics including the surface boundary conditions and the behavior of climatological air mass.

• Parasites, Hosts and Diseases
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• v.39 no.2
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• pp.143-150
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• 2001

The present study was designed to investigate polymorphism in Duffy binding protein (DBP) gene of Plasmodium vivax isolates of Korea. Thirty samples were obtained from P. vivax patients in Yonchon-gun, Kyonggi-do in 1998. The PCR products of the samples were subjected to sequencing and hybridization analyses of the regions II and IV of P. vivax DBP gene. Two genotypes, SK-1 and SK-2, were identified on the basis of amino acid substitution and deletion. The genotype of 10 isolates was SK-1 and that of 20 isolates was SK-2. Most of the predicted amino acids in the region ll of DBP gene were conserved between the Korean isolates and Belem strain except for 4-5 amino acid substitutions. In the region W of DBP, a 6-bp insert that was shown in the Sal-1 allele type was found in SK-1, and a 27-bp insert that was shown in the Papua New Guinea allele type was found in SK-2. In conclusion, the present findings suggest that two genotypes of P. vivax coexist in the endemic area of Korea.

• Parasites, Hosts and Diseases
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• v.54 no.3
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• pp.369-373
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• 2016

The 2 species of the genus Anoplocephala (Anoplocephalidae), A. perfoliata and A. magna, are among the most important equine cestode parasites. However, there is little information about their differences at the molecular level. The present study revealed that the mitochondrial (mt) genome of A. magna was 13,759 bp in size and 700 bp shorter than that of A. perfoliata. The 2 species includes 2 rRNA, 22 tRNA, and 12 protein-coding genes each. The size of each of the 36 genes was the same as that of A. perfoliata, except for cox1, rrnL, trnC, trnS2(UCN), trnG, trnH, trnQ, and trnP. In the full mitochondrial genome, the sequence similarity was 87.1%. The divergence in the nucleotide and amino acid sequences of individual protein-coding genes ranged from 11.1% to 16% and 6.8% to 16.4%, respectively. The 2 non-coding regions of the mt genome of A. magna were 199 bp and 271 bp in length, while the equivalent regions in A. perfoliata were 875 bp and 276 bp, respectively. The results of this study support the proposal that A. magna and A. perfoliata are separate species, consistent with previous morphological analyses.

• Parasites, Hosts and Diseases
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• v.56 no.5
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• pp.495-500
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• 2018

Trichuris suis infection in pigs is ubiquitous in intensive and extensive farms, which causes potential threat to human health. The objective of this research was to investigate the prevalence of T. suis in pigs in Hunan province. Total 2,267 fresh fecal samples distributed in 28 pig farms from 7 different administrative regions (Hunan province) were evaluated for the existence of T. suis eggs using saturated NaCl floating method. The average infection rate of T. suis in pigs was 8.91% in Hunan province. To determine genetic variation of the gained T. suis isolates in the present study, the internal transcribed spacer (ITS) regions from nuclear ribosomal DNA (rDNA) of 7 T. suis isolates were cloned and analyzed. Nucleotide diversities were 1.0-3.5% and 0-3.8% for ITS-1 and ITS-2, respectively. Phylogenetic analyses indicated that all isolates collected in the present study and T. suis available in Genbank generated a monophyletic clade. The present investigation revealed high infection rates of T. suis in pigs in Hunan province, which shed light on making effective measures to prevent and control T. suis infection in pigs in Hunan province.

• Parasites, Hosts and Diseases
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• v.56 no.2
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• pp.175-181
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• 2018

The giant roundworm Ascaris infects pigs and people worldwide and causes serious diseases. The taxonomic relationship between Ascaris suum and Ascaris lumbricoides is still unclear. The purpose of the present study was to investigate the genetic diversity and population genetic structure of 258 Ascaris specimens from humans and pigs from 6 sympatric regions in Ascaris-endemic regions of China using existing simple sequence repeat data. The microsatellite markers showed a high level of allelic richness and genetic diversity in the samples. Each of the populations demonstrated excess homozygosity (Ho0). According to a genetic differentiation index (Fst=0.0593), there was a high-level of gene flow in the Ascaris populations. A hierarchical analysis on molecular variance revealed remarkably high levels of variation within the populations. Moreover, a population structure analysis indicated that Ascaris populations fell into 3 main genetic clusters, interpreted as A. suum, A. lumbricoides, and a hybrid of the species. We speculated that humans can be infected with A. lumbricoides, A. suum, and the hybrid, but pigs were mainly infected with A. suum. This study provided new information on the genetic diversity and population structure of Ascaris from human and pigs in China, which can be used for designing Ascaris control strategies. It can also be beneficial to understand the introgression of host affiliation.

• Parasites, Hosts and Diseases
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• v.54 no.4
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• pp.431-437
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• 2016

The study of antigenic epitopes from Toxoplasma gondii has not only enhanced our understanding of the structure and function of antigens, the reactions between antigens and antibodies, and many other aspects of immunology, but it also plays a significant role in the development of new diagnostic reagents and vaccines. In the present study, T. gondii GRA6 epitopes were identified using bioinformatics tools and a synthetic peptide technique. The potential B cell epitopes of GRA6 predicted by bioinformatics tools concentrated upon 3 regions of GRA6, 1-20 aa, 44-103 aa, and 172-221 aa. Ten shorter peptides from the 3 regions were synthesized and assessed by ELISA using pig sera from different time points after infection. Three of the 10 peptides (amino acids 44-63, 172-191, and 192-211) tested were recognized by all sera and determined to be immunodominant B-cell epitopes of GRA6. The results indicated that we precisely and accurately located the T. gondii GRA6 epitopes using pig sera collected at different time points after infection. The identified epitopes may be very useful for further studies of epitope-based vaccines and diagnostic reagents.