Vasan Palanisamy;Sakthivel PC;Lane Pineda;Yanming Han
Animal Bioscience
/
v.36
no.11
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pp.1709-1717
/
2023
Objective: A pivotal study was designed to investigate the effect of Hydroxy (HYC) Cu, Zn, and Mn on egg quality and laying performance of chickens under tropical conditions. Methods: A total of 1,260 Babcock White laying hens (20-wk-old) were randomly assigned to one of 4 treatments with 15 replicates of 21 hens each in a Randomized Complete Block Design. The birds were reared for 16 weeks and were fed the corn-soybean meal diets supplemented with one of the following mineral treatments: T1, inorganic (INO, 15 ppm CuSO4, 80ppm MnSO4 and 80 ppm ZnO); T2, Hydroxy-nutritional level (HYC-Nut, 15 ppm Cu, 80 ppm Mn, 80 ppm Zn from Hydroxy); T3, Hydroxy-Low (HYC-Low, 15 ppm Cu, 60 ppm Mn, 60 ppm Zn from Hydroxy); T4, Hydroxy plus inorganic (HYC+INO, 7.5 ppm HYC Cu+7.5 ppm CuSO4, 40 ppm HYC ZnO+40 ppm ZnSO4, 40 ppm HYC Mn+40 ppm MnSO4). The egg production was recorded daily, while the feed consumption, feed conversion ratio (FCR) and egg mass were determined at the end of each laying period. The egg quality parameters were assayed in eggs collected over 48 h in each laying period. Results: Overall, no significant effect of treatments was observed on percent egg production, egg weight and FCR (p>0.05). Feed intake was significantly lower in birds fed Hydroxy plus inorganic (p<0.05) diet. The supplementation of HYC-Low significantly increased the egg mass compared to the other treatments (p<0.05). HYC supplementation alone or in combination with INO elicited a positive effect on shell thickness, shell weight, shell weight per unit surface area, yolk colour, albumen and yolk index for a certain period (p<0.05), but not throughout the whole laying period. Conclusion: Dietary supplementation of HYC-Low (15-60-60 mg/kg) showed similar effects on production performance and egg quality characteristics in laying hens as compared to 15-80-80 mg/kg of Cu-Zn-Mn from inorganic sources. This indicates that sulphate based inorganic trace minerals can effectively be substituted by lower concentration of hydroxyl minerals.
For the two-dimensional observation on the growth and development of CLonorchis sinensis, an image analysis system (IBAS 2000, Kontron, Germany) was used in this study. On 3, 6, 9, 12, 15, 21, 30 and 90 days of infection, 474 worms were collected from rats infected with 50 metacercariae each. The overall recovery rate was 31.6%. The worms grew rapidly in their length and width up to 30 days of infection and then, did slowly to 90 days of infection. However, the growth pattern of body area was not similar to that of body length and width, because the body area increased continuously even after 30 days of infection. With the image analysis system, the sizes of irregular-shaped genital organs were measured easily, which showed sigmoidal growth patterns . The growth rate of genital organs increased rapidly. until 21 days of infection when the uterus was filled with numerous eggs, and then gradually slowed down to 90 days. There was no difference in growth pattern between the anterior and posterior testis. The seminal receptacle , however, showed an abrupt increase in size between 15 to 21 days of infection when it was filled with condensed semen. Therefore, the growth pattern of seminal receptacle might be used as another criteria to estimate the extent of sexual maturation of C. sinensis. From this study, it is suggested that image analysis system is very useful to reveal the growth and development pattern of C. sinensis, especially of their internal organs.
Riboldi, Emeline;Carvalho, Flavio;Romao, Pedro Roosevelt Torres;Barcellos, Regina Bones;Bello, Graziele Lima;Ramos, Raquel Rocha;de Oliveira, Rosemari Terezinha;Araujo, Joao Pessoa Junior;Rossetti, Maria Lucia;Dallegrave, Eliane
Parasites, Hosts and Diseases
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v.56
no.1
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pp.11-19
/
2018
In Brazil, visceral leishmaniasis (VL) is expanding and becoming urbanized, especially in non-endemic areas such as the State of Rio Grande do Sul. Considering that infected dogs are the main reservoir for zoonotic VL, this study evaluated the prevalence of canine visceral leishmaniasis (CVL) in the metropolitan area of Porto Alegre, a new area of expansion of VL in Brazil. Serum and plasma from 405 asymptomatic dogs from the municipalities of Canoas (n=107), $S\tilde{a}o$ Leopoldo (n=216), and Novo Hamburgo (n=82) were tested for CVL using immunochromatographic ($DPP^{(R)}$) and ELISA $EIE^{(R)}$ assays (2 assays officially adopted by the Brazilian government for the diagnosis of CVL) and real-time PCR to confirm the results. There was no agreement among serological and real-time PCR results, indicating that the Leishmania infection in asymptomatic animals with low parasite load, confirmed by negative parasitological tests (smears and parasite culture), need to be evaluated by molecular methods. The prevalence of LVC in the metropolitan region of Porto Alegre, confirmed by real-time PCR was 4% (5.6% in Canoas and 4.6% in $S\tilde{a}o$ Leopoldo). The use of molecular method is essential for accurate diagnosis of CVL, especially in asymptomatic dogs in non-endemic areas.
Proceedings of the Korea Water Resources Association Conference
/
2002.05a
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pp.43-50
/
2002
Accurate quantitative forecasting of rainfall for basins with a short response time is essential to predict streamflow and flash floods. Previously, neural networks were used to develop a Quantitative Precipitation Forecasting (QPF) model that highly improved forecasting skill at specific locations in Pennsylvania, using both Numerical Weather Prediction (NWP) output and rainfall and radiosonde data. The objective of this study was to improve an existing artificial neural network model and incorporate the evolving structure and frequency of intense weather systems in the mid-Atlantic region of the United States for improved flood forecasting. Besides using radiosonde and rainfall data, the model also used the satellite-derived characteristics of storm systems such as tropical cyclones, mesoscale convective complex systems and convective cloud clusters as input. The convective classification and tracking system (CCATS) was used to identify and quantify storm properties such as life time, area, eccentricity, and track. As in standard expert prediction systems, the fundamental structure of the neural network model was learned from the hydroclimatology of the relationships between weather system, rainfall production and streamflow response in the study area. The new Quantitative Flood Forecasting (QFF) model was applied to predict streamflow peaks with lead-times of 18 and 24 hours over a five year period in 4 watersheds on the leeward side of the Appalachian mountains in the mid-Atlantic region. Threat scores consistently above .6 and close to 0.8 ∼ 0.9 were obtained fur 18 hour lead-time forecasts, and skill scores of at least 4% and up to 6% were attained for the 24 hour lead-time forecasts. This work demonstrates that multisensor data cast into an expert information system such as neural networks, if built upon scientific understanding of regional hydrometeorology, can lead to significant gains in the forecast skill of extreme rainfall and associated floods. In particular, this study validates our hypothesis that accurate and extended flood forecast lead-times can be attained by taking into consideration the synoptic evolution of atmospheric conditions extracted from the analysis of large-area remotely sensed imagery While physically-based numerical weather prediction and river routing models cannot accurately depict complex natural non-linear processes, and thus have difficulty in simulating extreme events such as heavy rainfall and floods, data-driven approaches should be viewed as a strong alternative in operational hydrology. This is especially more pertinent at a time when the diversity of sensors in satellites and ground-based operational weather monitoring systems provide large volumes of data on a real-time basis.
Ecological studies of Aedes togoi, the vector of malayan filariasis, were ci,wind out at Tolsando, Yosu and Sokcho area in 1991. The adult population of Aedes togoi was continuously appeared from the first week of April to the end of November showing the highest density in .truly. The larvae of Aedes togoi were found at rock pools from March to December in Sokcho area and the density was highest in July and August, whereas in the southern coastal area (Yosu), the larvae were found throughout the year and the density was the highest in Tune. The rate of larvae inhabited below 0.5% salinity was 45.7% in Sokcho and 51.7% in Yosu. The feeding activity of Aedes togoi was nocturnal, with the peak period of 01 :00-03:00 hours. Indoor feeding activities were slightly higher than outdoors showing the biting ratio of 1 :0.8 (indoor: outdoor). The average number of Aedes togoi attracted to CO2 gas was 8.5 whereas 117 Anophelei sinensis was attracted. The result indicates that CO2 is not an effective attractant for host seeking of Aedes togoi compared to Anopheles sineteis. The most common place was bedroom with 54.5% of total collections and next to stock place (18.2%), floor(9.1%) and kitchen (9.1%).
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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v.4
no.1
/
pp.50-62
/
1999
Sediment core samples recovered from the Korea Deep Ocean Study (KODOS)-97 area were divided into two or three units according to their distinct changes in sediment colors and chemical and physical properties. Analyses of radiolarian faunas in the sediments and $^{10}Be$ ratios in each unit were performed to reveal stratigraphic and paleoceanographic history of the study area. In the upper part of the sediments, Tertiary radiolarians were mixed at various proportions with Quaternary assemblages probably by reworking process of bottom current and benthic animals. Dissolution of radiolarians was severe in deeper depth and in the Unit III, only few of the fragments of corroded Tertiary radiolarians were detectable. The mid layer of the Unit I belonged to Collosphaera invaginata Zone, the time period of 0.21 Ma. The Unit II belonged to Collosphaera tuberosa Zone with the time period younger than 0.42 Ma which was observed above the Stylatractus universus Zone. The Unit III is assigned to Tertiary, which is younger than the Late Eocene. Composition analyses of radiolarian assemblage and $^{10}Be$ ratio data indicated hiatus periods of more than 3 My between late of Middle Miocene and Pliocene resulting from erosion and dissolution caused by Antarctic Bottom Water. Stratigraphic evidence from radiolaria was well correlated with $^{10}Be$ data. Sedimentation rate during Quaternary can be suggested as 0.15-0.5 mm per 1000 years. Dominance of warm-water radiolaria species and the results reflected minimum climatic changes of tropical conditions.
Journal of the Korean Association of Geographic Information Studies
/
v.20
no.2
/
pp.1-16
/
2017
This study was conducted to analyze the urban heat island(UHI) intensity of South Korea by using Moderate Resolution Imaging Spectroradiometer(MODIS) satellite imagery. For this purpose, the metropolitan area was spatially divided according to land cover classification into urban and non-urban land. From the analysis of land surface temperature(LST) in South Korea in the summer of 2009 which was calculated from MODIS satellite imagery it was determined that the highest temperature recorded nationwide was $36.0^{\circ}C$, lowest $16.2^{\circ}C$, and that the mean was $24.3^{\circ}C$, with a standard deviation of $2.4^{\circ}C$. In order to analyze UHI by cities and counties, UHI intensity was defined as the difference in average temperature between urban and non-urban land, and was calculated through RST1 and RST2. The RST1 calculation showed scattered distribution in areas of high UHI intensity, whereas the RST2 calculation showed that areas of high UHI intensity were concentrated around major cities. In order to find an effective method for analyzing UHI by cities and counties, analysis was conducted of the correlation between the urbanization ratio, number of tropical heat nights, and number of heat-wave days. Although UHI intensity derived through RST1 showed barely any correlation, that derived through RST2 showed significant correlation. The RST2 method is deemed as a more suitable analytical method for measuring the UHI of urban land in cities and counties across the country. In cities and counties with an urbanization ratio of < 20%, the rate of increase for UHI intensity in proportion to increases in urbanization ratio, was very high; whereas this rate gradually declined when the urbanization ratio was > 20%. With an increase of $1^{\circ}C$ in RST2 UHI intensity, the number of tropical heat nights and heat wave days was predicted to increase by approximately five and 0.5, respectively. These results can be used for reference when predicting the effects of increased urbanization on UHI intensity.
Pre-school children were examined for the presence of Enterobius vermicularis egg by perianal swab method. We visited 67 nurseries and 25 kindergartens between January, 1999 and April, 1999 in Chunchon, Korea. Of the 4,711 children examined, 434 (9.2%) were found to be positive for E. vermicularis egg. The egg positive rates of boys and girls were 10.1% and 8.1%, respectively. The rates were 7.8% in kindergartens and 9.7% in nurseries. Positive rate of 50 institutions was less than 10%. Rate in 35 institutions was from 10% to less than 20%. Rate in 7 institutions was equal to or over 20%. Out of 1,113 children examined twice, 28 (2.5%) children were positive consecutively, 53 (4.8%) were positive with negative conversion, 47 (4.2%) were negative with positive conversion, and 985 (88.5%) were consecutively negative. It means that in the low endemic area of enterobiasis with around 10% positive rate, the two consecutive examinations may increase the egg detection rate of 4.2-4.8%. The small number of consecutive egg positive children also suggests that the worm burden of the positive children might be low. Since the egg positive rate of that a9e group in this City increased from 1.85% (1997), and 3.0% (1998), the more intensive regular control should be executed.
A small-scale epidemiological survey was undertaken during 1997-1998 on the residents along the Osib-chon (Stream), Samchok-shi (City), Kangwon-do (Province), to evaluate the status of Metagonimus yokogawai infection. A total of 165 fecal samples was collected and examined by cellophane thick smear and formalin-ether sedimentation techniques. The egg Positive rate of M. yokogawai was 29.7%, showing a remarkable difference between males (46.6%) and females (16.3%). To obtain the adult flukes of M. yokogawai, 11 egg positive persons were treated with praziquantel and purged with magnesium sulfate. A total of 242,119 adult flukes (average 22,010 per person, 367 119,650 in range) was collected from diarrheic stools, all of which were identified as M. yokogawai. The results show that M. yokogawai is still highly endemic in this area.
Park, Jae-Won;Cheong, Hae-Kwan;Honda, Yasushi;Ha, Mina;Kim, Ho;Kolam, Joel;Inape, Kasis;Mueller, Ivo
Environmental Analysis Health and Toxicology
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v.31
/
pp.3.1-3.11
/
2016
Objectives This study was conducted to describe the regional malaria incidence in relation to the geographic and climatic conditions and describe the effect of altitude on the expansion of malaria over the last decade in Papua New Guinea. Methods Malaria incidence was estimated in five provinces from 1996 to 2008 using national health surveillance data. Time trend of malaria incidence was compared with rainfall and minimum/maximum temperature. In the Eastern Highland Province, time trend of malaria incidence over the study period was stratified by altitude. Spatio-temporal pattern of malaria was analyzed. Results Nationwide, malaria incidence was stationary. Regionally, the incidence increased markedly in the highland region (292.0/100000/yr, p =0.021), and remained stationary in the other regions. Seasonality of the malaria incidence was related with rainfall. Decreasing incidence of malaria was associated with decreasing rainfall in the southern coastal region, whereas it was not evident in the northern coastal region. In the Eastern Highland Province, malaria incidence increased in areas below 1700 m, with the rate of increase being steeper at higher altitudes. Conclusions Increasing trend of malaria incidence was prominent in the highland region of Papua New Guinea, while long-term trend was dependent upon baseline level of rainfall in coastal regions.
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