• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.1
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• pp.117-125
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• 2007

Neuronal ischemia is a pathological process caused by a lack of oxygen (anoxia) and glucose (hypoglycemia), resulting in neuronal death. It is believed that apoptosis is one of the mechanisms involved in ischemic cell death. Neuronal apoptosis is a process characterized by nuclear DNA fragmentation, changes of plasma membrane organization. To elucidate the mechanism of neuronal death following ischemic insult and to develop neuroprotective effects of Daebowonjeon(DBWJ) against ischemic damage, in vitro models are used. In vitro models of cell death have been devloped with pheochromocytoma (PC12) cell, which have become widely used as neuronal models of oxidative stress, trophic factor, serum deprivation and chemical hypoxia. Using a special ischemic device and PC12 cultures, we investigated an in vitro model of ischemia based on combined Oxygen and Glucose Deprivation (OGD) insult, followed by reoxygenation, mimicking the pathological conditions of ischemia. In this study, Daebowonjeon rescued PC12 cells from Oxygen-Glucose Deprivation (OGD)-induced cell death in a dose-dependent manner The nuclear staining of PC12 cells clearly showed that DBWJ attenuated nuclear condensation and fragmentation which represent typical neuronal apoptotic characteristics. DBWJ also prevents the LDH release and induction of Hypoxia Inducing Factor (HIF)-1 by OGD-exposed PC12 cells. Furthermore, DBWJ reduced the activation of polyADP-ribose polymerase (PARP) by OGO-exposed PC12 cells. These results suggest that apoptosis is an important characteristic of OGD-induced neuronal death and that oriental medicine, such as DBWJ, may prevent PC12 cell from OG D-induced neuronal death by inhibiting the apoptotic process.

• Journal of Korean Society on Water Environment
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• v.28 no.4
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• pp.551-560
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• 2012

The objectives of this study were to elucidate spatio-temporal heterogeneity of water chemistry and develop empirical models using trophic variables in Daechung Reservoir during 2005-2010 along with in situ tests of nutrient enrichment bioassays (NEB). The relations of water quality parameters in regard to precipitation showed that seasonal and interannual fluctuations of biological oxygen demand (BOD), total nitrogen (TN) and pH were minor, whereas conductivity, suspended solids (SS), and total phosphorus (TP) were largely varied in response to the magnitude of rainfall. The CHL maxima occurred immediately after the spate of TP during the high flow, indicating that phytoplankton growth was directly controlled by phosphorus. Empirical linear models of CHL-TP indicated that the variation of CHL in premonsoon was accounted 60% ($R^2$ = 0.60, p < 0.05, n = 54) by TP. In the mean time, empirical models of annual CHL-TN showed that the variation of CHL was weakly accounted ($R^2$ = 0.16, p < 0.001) by TN and more strongly ($R^2$ = 0.44, p < 0.001) by TP. Thus, the variation of CHL was more explained by the variation of TP than TN. In situ tests of Nutrient Enrichment Bioassays (NEBs) showed that the growth of CHL was greater in the P-treatments (as $PO_4-P$) than the control and N-treatment (as $NO_3-P$). Overall, our results suggest that phosphorus was aprimary limiting nutrient controlling the seasonal phytoplankton growth, based on the in situ experiments of NEBs.

• Journal of Ecology and Environment
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• v.39 no.1
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• pp.99-110
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• 2016

The objective of this study was to analyze chemical water quality, ecological characteristics of fish compositions, and ecosystem health before- (B_wc; 2008-2009) and after-the weir construction (A_wc; 2011-2012) at Juksan Weir and Seungchon Weir of Yeongsan River watershed. Suspended solids (SS) and chlorophyll-a (Chl-a) in Juksan Weir increased, whereas nutrients such as total nitrogen (TN) and total phosphorus (TP) decreased in the epilimnetic water. In Juksan and Seungchon weirs, fish species distribution analysis in the periods of B_wc and A_wc showed that sensitive species were rare and tolerant species were dominant in the community. In the analysis of trophic guild, relative abundance of carnivore species are increased to 22% and 12%, respectively, after the constructions of Seungchon Weir and Juksan Weir. Mann-Whitney U-tests of nonparametric statistical analysis indicated that omnivore and carnivore species had significant differences (p < 0.05) between the B_wc and A_wc. The massive population growth of an exotic species, Micropterus salmoides, was evident in Seungchon Weir to influence on the structures of fish communities. The model values of mean Index of Biological Integrity (IBI), based on fish assemblages, were < 15, which indicates "poor" condition in the river health, and the significant difference of IBI values was not found between the B_wc and A_wc.

• Korean Journal of Ecology and Environment
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• v.40 no.4
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• pp.527-536
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• 2007

In this study, we applied 10-metric health assessment model, based on the Index of Biological Integrity (IBI) during 2006 in the Changwon Stream, which is located in the Changwon city, Gyeongnam province, S. Korea, and then compared with water quality data. The Index of Biological Integrity (IBI) in the Changwon Stream varied from 18 to 38 in the watershed depending on the sampling location and averaged 30.3 (n=6) during the study. Analysis of tolerance guilds showed that the proportion of sensitive species was 13%, but tolerant and intermediate species were 34% and 53%, respectively. Qualitative Habitat Evaluation Index (QHEI) averaged 43.3 (range: 65-104, n=6) indicating non-supporting condition, based on the criteria of U.S. EPA (1993). Values of QHEI showed a typical longitudinal decreases from the headwater reach to the downstream location, except for Site 1 with a low QHEI value by artificial habitat by concrete construction. Minimum QHEI was found in Site 4 where fish diversity was minimal. Conductivity increased continuously along the gradients and especially showed abrupt increases in the downstream sites along with turbidity. Stream ecosystem health of IBI matched to the values of QHEI except for S6. Low IBI values in the sites 4 and 5 was considered to be a result of combined effects of chemical pollutions and habitat degradations. Our results support the hypotheses of Plafkin et ai. (1989) that physical habitat quality directly influences the trophic structure and species richness, and is closely associated with IBI values.

• Journal of Ecology and Environment
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• v.38 no.2
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• pp.195-211
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• 2015

The objectives of this research were to determine mean and maximum tolerance ranges of Carassius auratus ($C_a$) and Cyprinus carpio ($C_c$) populations on various physico-chemical parameters and ecological indicator metrics. Little is known about chemical tolerance ranges of the two species, even though these species are widely distributed species in aquatic ecosystems. Maximum tolerance ranges of $C_a$-population to total nitrogen (TN) and total phosphorus (TP) were $20.3mgL^{-1}$ and $2.0mgL^{-1}$, respectively. Optimal ranges of TN and TP in the $C_a$-population were $1.7-5.0mgL^{-1}$ and $0.06-0.30mgL^{-1}$, respectively. Such nutrient regimes of the $C_a$-population were evaluated as hypereutrophy, indicating high tolerance limits. The $C_c$-population had similar ecological characteristics to $C_a$-population, but the mean tolerance ranges of TN, TP, BOD, and COD were significantly (p < 0.05) greater than the $C_a$-population. Ecological patterns of trophic composition and tolerance guilds in the $C_a$-population were similar to those of the $C_c$-population. The model value of Index of Biological Integrity (IBI) of the habitat where C. auratus and C. carpio co-occurred averaged $15.0{\pm}4.3$ and $12.9{\pm}3.6$, respectively. Based on the modified criteria of the United States Environmental Protection Agency (Klemm et al. 1993), it indicated poor ecological health of both species. These results suggest that both species are highly tolerant to chemical and physical habitat conditions of waterbodies, and that the chemical tolerance range of $C_c$-population was higher than $C_a$-population.

• Journal of Korean Society on Water Environment
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• v.20 no.6
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• pp.589-596
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• 2004

In this study, the reduction rates of nutrients were suggested to prevent eutrophication on the Hwaong reservoir in the year of 2008 and 2012. With EPA's WASP6 model, future water quality were simulated. In 2008, T-N would be 1.36mg/L and T-P 0.100mg/L on average. ; In 2012, T-N 2.66mg/L and T-P 0.128mg/L. With all the water quality management plans that the government authorities are carrying out, these results indicate that the reservoir would be reach the eutrophic or hypertrophic state according to the Vollenweider's trophic states. Therefore, the Hwaong reservoir requires additional plans for nutrients management. Here, the target water quality to prevent eutrophication of the reservoir sets into mesotrophic state ; T-N 0.475mg/L and T-P 0.02mg/L.(median of Vollenweider index for mesotropphic state) The reduction rates of nutrients on Namyang and Eoeun streams were estimated with uniform treatment method to meet the goal. The results showed that nutrients from two streams should be reduced up to 78% in 2008, and 84% in 2012. Since the ratio of T-N/T-P would be higher than 16 at target years, T-N was not considered as the limiting factor and was not reduced.

• Korean Journal of Ecology and Environment
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• v.42 no.4
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• pp.452-464
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• 2009

The objectives of this study were to evaluate spatial and temporal variability of water quality in 10 reservoirs and identify the key nutrients (N, P) influencing chlorophyll-a (CHL) along with analysis of empirical models and zonal patterns of total phosphorus (TP) and CHL. We analyzed total nitrogen (TN), TP, CHL, water clarity (Secchi depth, SD), and evaluated potential limiting nutrient using ambient N:P ratios and previous criteria of ambient nutrients. Water clarity and CHL varied largely depending on the seasonal monsoon and type of reservoir, but trophic state was diagnosed as eutrophy, base on mean CHL in most reservoirs. The peak of TP did not match the contents of CHL due to rapid flushing during the high run-off period. In the reservoir of DR, regression coefficient in the $P_r$ was 0.510 but was 0.159 in the $M_o$, while the TP-CHL relation in the YR increased during the monsoon compared to the premonsoon. The regression coefficient in the $P_r$ was not statistically significant but the value of $M_o$ was 0.250. TP showed similar longitudinal zonal gradients among the reservoirs of DR, YR and JR. Empirical models of TP-CHL, based on overall data, showed that CHL was determined by phosphorus($R^2=0.244$, p=0.0019). Regression analysis of CHL-SD showed a stronger linear fit ($R^2=0.638$, p<0.001) than the TP-CHL model.

• Journal of Korean Society on Water Environment
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• v.34 no.5
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• pp.470-486
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• 2018

This study is to investigate the allochthonous load and water quality of a typical river-reservoir, Paldang during spring (March ~ May) of 17 years (2001 ~ 2017). Phosphorus loading from point sources seems to have been reduced by 74 % in the 2010s. As a result, trophic state of the Paldang reservoir, eutrophic during the 2000s, has returned to the lmesotrophic state. Along with decrease in phosphorus concentration, standing crops of algae (Chl.a) decreased, and concentration of biodegradable organic material decreased to the past level. Concentration of total suspended solids has decreased, and it is due to the decrease of phytoplankton standing crops since the mid-2000s. As transparency increased, it is estimated that euphotic area increased by 22 % and euphotic capacity expanded by 27 %. In the river/transition zone of Paldang, concentration of organic matter increases slightly due to algal growth, but concentration of all water quality items decreases in the lacustrine zone. Although algal growth rate revealed positive correlation with concentration of phosphorus, it was insignificant. Algal growth appeared to be dependent on renewal of phosphorus by flow, than either flow rate or phosphorus concentration. The empirical model including inflow phytoplankton concentration fit well with observed values, and indicates the Paldang reservoir is greatly influenced by allochthonous loads.

• Fisheries and Aquatic Sciences
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• v.26 no.4
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• pp.268-281
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• 2023

Coral reef fisheries are prominent for the archipelagic countries' food sufficiency and security. Studies showed that fish abundance and biomass are affected by biophysical variables. The present study determines which biophysical variables are important predictors of fish diversity, abundance, and biomass. The study used available monitoring data from the Indonesian Research Center for Oceanography, the National Board for Research and Innovation. Data were collected from 245 transects in 19 locations distributed across the Indonesian Archipelago, including the eastern Indian Ocean, Sunda Shelf (Karimata Sea), Wallacea (Flores and Banda Seas), and the western Pacific Ocean. Principal component analysis and multiple regression model were administered to 13 biophysical metrics against 11 variables of coral reef fishes, i.e., diversity, abundance, and biomass of coral reef fishes at three trophic levels. The results showed for the first time that the covers of other fauna, coral rubbles, and soft corals were the three most important predictor variables for nearly all coral reef fish variables. Other fauna cover was the important predictor for all 11 coral reef fish variables. Coral rubble cover was the predictor for ten variables, but carnivore fish abundance. Soft coral cover was a good predictor for corallivore, carnivore, and targeted fishes. Despite important predictors for corallivore and carnivore fish variables, hard coral cover was not the critical predictor for herbivore fish variables. The other important predictor variables with a consistent pattern were dead coral covered with algae and rocks. Dead coral covered with algae was an important predictor for herbivore fishes, while the rock was good for only carnivore fishes.

• Korean Journal of Environment and Ecology
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• v.25 no.5
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• pp.747-759
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• 2011

The purpose of this study was to compare the trophic structures and the energy flows in the Lake Namyang and the lower reaches of the Nakdong River using the Ecopath model. The sampling and analyses were carried out at 6 sampling sites of the Lake Namyang and the lower reaches of the Nakdong River respectively on March and November in 2007. As a result, the Lake Namyang was consisted of producers(Detritus, Macrophytes, Phytoplankton), primary consumers(Zooplankton, Zoobenthos, Carassius cuvieri, Carassius auratus, Other fishes) and secondary consumer(Cyprinus carpio, Pseudobagrus fulvidraco) and the lower reaches of the Nakdong River was consisted of producers(Detritus, Macrophytes, Phytoplankton), primary consumers (Zooplankton, Zoobenthos, Cyprinus carpio, Hemibarbus labeo, Other fishes) and secondary consumer (Micropterus salmoides). The food-chain length of the Lake Namyang was relatively short when compared with the lower reaches of the Nakdong River. The shortness of food-chain length in the Lake Namyang could be attributed to the low biomass of the top predators. The total system throughput of the lake Namyang was estimated at 14.3 kg $m^{-2}\;year^{-1}$ including a consumption of 39.0%, exports of 21.0%, respiratory flows of 12.0% and flows into detritus of 28.0% and the total system throughput of the lower reaches of the Nakdong River was estimated at 2.8 kg $m^{-2}\;year^{-1}$ including a consumption of 52.0%, exports of 9.1%, respiratory flows of 18.0% and flows into detritus of 20.9% in the lower reaches of the Nakdong River.