• Korean Journal of Plant Resources
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• v.16 no.1
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• pp.61-73
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• 2003

External and anatomical characters such as stem, leaf, ovary, calyx lobe, ultrastructure of stigma epidermis of leaf blade and midvein, corolla lobe, seed coat and pollen were examined on Pterygocalyx volubilis and Tripterospermum japonicum of Korean Gentianaceae. Fruit characters(capsule and berry), absent or not of corona and pollen characters(reticulate and striate) has been used by identification characters of two taxa. But study results, external characters(root, leaf, calyx lobe, stigma, anther), internal characters(calyx love, ovary) and ultrastructure characters(epidermis o leaf blade and midvein, corolla lobe, seed coat) were useful for species identification. Taxonomic relationships among problematic taxa were discussed.

• Journal of Plant Biology
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• v.24 no.1
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• pp.27-30
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• 1981

This paper is proposed to describe three new species, two varieties and one forma of flowering plants from central Korea and the Island of Cheju in southern Korea. The taxa of new plants are as follows: Astragalus koraiensis Y. Lee, sp. nov.; Astragalus nakaianus Y. Lee, sp. nov.; Commelina minor Y. Lee & Oh Y., sp. nov.; Cirsium rhinoceros Nakai var. albiflorum Y. Lee, var. nov.; Tripterospermum japonicum (Sieb. & Zucc.) Max. var. albiflorum Y. Lee, var. nov.; Adenophora remotiflora (Sieb. & Zucc.) Miq. form. albiflora Y. Lee, form. nov.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.178-178
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• 2004

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.13-17
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• 2009

Various plant growth regulators were tested for shoot proliferation of Tripterospermum japonicum, a rare and endangered species. Among the six different media tested, MS medium was the best for the shoot growth. Whereas BA, upto 3 mg/L, significantly increased shoot proliferation rate, it suppressed the rate at higher levels. Neither kinetin nor TDZ was so effective in proliferating shoots as BA. As for rooting, TDZ strongly inhibited it even at very low concentration though spontaneous rooting was frequently observed from the proliferated shoots during culture of lower concentration BA or kinetin. In contrast, shoot elongation was significantly promoted by $GA_3$. More than 90% of the proliferated plantlets could be transplanted via cuttings into pots containing artificial soil mixture where they rooted and resumed normal growth. Most of the plants bloomed to bear fruits in the following year.

• Journal of Environmental Science International
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• v.12 no.2
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• pp.125-132
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• 2003

This study was executed by China along with the Tumen river, which formed border of North Korea and China. As a result 91 family, 282 genera, 363 species, 57 variety 4 species, total 424 taxa of vascular plants were identified. The examination area was include the region that had various vegetation such as upper swampy land, seashore dune, a river, a high mountain grassy plain, deciduous tree zone, evergreen coniferous forest zone. The followings are representative plants, which found in an examination area but not in South Korea. Picea koraiensis, Larix gmelini var. principis-ruprechtii, Betula playphylla, Ulmus pumila, Populus koreana, Ledum palustre var. diversipilosum, Rhododendron parvifolium, Salix brachypoda, Phacelurus latifolius, Carex gotoi, Carex capricornis, Calla palustris, Aquilegia flabellata var. pumila, Lilium davuricum, Astragalus membranaceus, Trifolium lupinaster, Ledebourieila seselodeds, Polemonium racemosum, Scutellaria baicalensis, Linnaea borealis, Thladiantha dubia, Inula helenium, Trollius hondoensis, Hypocaeris ciliata, Aconitum kirinense, Aconitum arcuatum, Potentilla viscosa var. macrophylla. And there are 21 rare and endangered species, which Korean Forest Sevice designated, such as Aconitum koreanum, Acorus calamus var. angustatus, Polygonatum stenophyllum, Drosera rotundifolia, Iris setosa, Jeffersonia dubia, Lilium davuricum, Lilium cernum, Paeonia obovata, Penthorum chinense, Phacelurus latifolius, Rosa marretii, Sparganium stoloniferum, Thymus quinquecostatus, Trillium tschonoskii, Utricularia japonica, Tripterospermum japonicum, Vaccinium uliginosum, Vaccinium vitis-idaea, etc. Especially, plants like, Acanthopanax senticosus, Jeffersonia dubia and Lillum cernum which have high values of ornamental, and medical use are in an extinction crisis. High swampy land was formed to upper stream of Tumen river. It showed representative vegitation, like Carex sp., Scirpus radirans, Eriophorum latifolium. Wetlands plant and a dune plant were mixed in down stream area of Tumen river. On a dune, seashore plants, such as Carex kobomugi, Salsola collina, Raphanus sativus var. raphanistroides, Rosa rugosa, Scutellaria strigillosa, Linaria japonica, Phacelurus latifolius were appeared frequently.

• Journal of Plant Biotechnology
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• v.35 no.3
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• pp.215-221
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• 2008

Effects of light generated by LEDs on shoot growth and rooting of Tsuru-rindo(Tripterospermum japonicum) were evaluated. Apical shoots(one or two node with 3-4 leaves) were cultured on MS basal medium with 3% sucrose and maintained for four weeks under five different light qualities: fluorescent lamp(F), 100% red LED(R), 70% red LED+30% blue LED(R7B3), 50% red LED+50% blue(R5B5), or 100% blue LED(B). Rooting was promoted by both red light and fluorescent lamp, and the effect was further promoted under the ventilation. Red light enhanced shoot node elongation, whereas blue light appeared to suppress it. Growth of shoots and leaves were enhanced under the ventilation irrespective of the different light qualities. Under the ventilated condition, total fresh weight of plants was highest in R7B3 LED as 257.7 mg per plant. Dry matters, which are used for index of plant growth, were lowest under red light, whereas it was highest under blue light. The dry matter was inclined to getting higher by ascending the ratio of blue light and red light. Total chlorophyll content was highest in both R7B3 LED and R5B5 LED under ventilation as 29.5 and 31.2, respectively. Above results suggest that light quality optimization could be an important factor to foster in vitro growth of the species. Ventilation treatment appeared to be another important factor to induce normal shoot growth and rooting.