• Journal of The Korean Society of Grassland and Forage Science
• /
• v.18 no.3
• /
• pp.195-204
• /
• 1998

This study was designed to investigate the effects of antagonistic microorganism, Bacillus subtilis, on the growth of forage seedlings in repeated cultivation soils and unrepeated cultivation soils. The field experiment was wnducted in pots in a vinyl house using repeated and unrepeated cultivation soils. Forage types were 'Suwon 19' wrn(Zea mqs L.), 'Califbmia' white clover(Tr~oIium repens L.) and 'Fawn' tall fescue (Festuca arundianacea Schreb.). Samples of white clover and tall fescue were taken h m each pot at 36 days after seeding. Samples of wm were examined at 50 days after seeding. The most active antagonistic bacterium was isolated h m forage rhizosphere soil, and selected by reference to it's antagonistic ability on the growth of pathogenic fungi, Rhizoctonia solmi and Fusarium oxyspomm, and it was identified as Bacillus subtilis. This strain strongly suppressed the growth of fungal pathogens among isolated rhizobacteria. The dry weight of forage shoots and roots cultivated in unrepeated cultivation soils was higher than that cultivated in repeated cultivation soils. The dry weight of forage was positively affected by the inoculation of the antagonistic bacterium, Bacillus subtilis, in both repeated cultivation soils and unrepeated cultivation soils. In conclusion, the growth of forage was more affected by the inoculation of the antagonistic bacterium in unrepeated cultivation soils than that in repeated cultivation soils, and bacterization of forage with B. subtilis resulted in an inrreased yield.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.28 no.2
• /
• pp.119-128
• /
• 2008

The object of this experiment was to investigate the food habits of Korean native goats fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources: 5 species (Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass, legume sources: 5 species (Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources: 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources: 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea (Roxb.) Wight and Rumex crispus), and native grass sources: 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Ten Korean native goats were selected which had nearly the same body weight(average 30.6kg, 남) as experimental animals. Experimental roughages were prepared by 0.5kg per 1 species fed to Korean native goats, and the experiment was carried out from 08:00 to 20:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period (p<0.05). The Korean native goats ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species order by Korean native goats was observed like this: Quercus serrata Thunb., Quercus aliena Blume, Trifolium repens, and Pinus densiflora and Artemisia princeps Pampanini which was lower intake compared to other domestic herbivores. On the other hand, compared to each roughage source, total intake amount by Korean native goats was showed as browse(29.9%), legumes(23.0%), weeds(21.6%), grasses(21.5%) and native grasses(4.0%), respectively. Based on the result, the food habit of Korean native goats seems to be closer to browser type.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.31 no.3
• /
• pp.336-349
• /
• 1986

This study was conducted to examine the plant succession on the improved pasture of the mountain districts around Mt. Halla in Cheju Island. For this study, the researcher had investigated for ten years the improved pasture which had been used for grazing from 1976 to 1985. The pasture was reclaimed from native grassland. The mixed seeds of 17kgs' Dactylis glomerata, 7kgs' Festuca arundinacea, 2kgs' Lolium multiflorum and 2kgs' Trifolium repens were sowed per ha. The results of this study are the followings; The annual changes in the number of plant species were observed. 37 weed species were found in 1976 and increased year after year to 151 species in 1985. The changes in the distribution of annual and perennial plant, and one species of arbor were found in 1976 and increased respectively year by year to 56 species (annual plant), 95 species (perennial plant) and 9 species (arbor) in 1985. The average plant height of introduced grasses by year increased from 38.05cm (1976) to 47.30cm (1978) and decreased from 40.50cm (1979) to 10.36cm in 1985 (y =-0.501x$^2$+1.609x + 41.946). While the average plant height of invading weeds increased from 26.61cm to 42.84cm (y=-0.80$\chi$$^2$＋2.540$\chi$＋27.570) between 1976 and 1985. The density of introduced grasses was 70.90% in 1976 and was reduced to 0.49% in 1985 (y =-0.501$\chi$$^2$+1.609$\chi$+41.946); while that of introduing weeds was 29.10% in 1976 and was increased to 99.51% in 1985 (y=-0.080$\chi$$^2$＋2.540$\chi$＋27.570). The coverage of introduced grasses by year increased gradually from 72.8% (1976) to 74.86% (1978) and decreased from 43.01% (1979) to 1.21% in 1986 while that of intruding weeds developed a tendency to increase every year. Their coverage in 1976 was 22.09% and increased to 98.78% in 1985. The weight of introduced grasses by year increased from 2,808kg (1976) to 3,535kg (1978) per l0a and after 1979 decreased gradually from 2,326kg (1978) to 35kg per l0a in 1985. That of intruding weeds increased yearly from 308kg in 1976 to 3,178kg in 1985. The type of annual vegetation were changed as follows; Year Type 1976-1978 Dactylis glomerata / Trifolium repens type 1979 Trifolium repens / Imperata cylindrica type 1980-1982 Imperata cylindrica / Zoysia Japonica type 1983 Imperata cylindrica / Pteridium aquilinum type 1984-1985 Imperata cylindrica / Miscanthus sinensis type The plants whose plant height, coverage, density, and weight increased year after year were Imperate cylind-rica, Zoysia japonica, Pteridium equilinum, Miscanthus sinensis, Cirsium japonicum, Erigeron canadensis, Artemisia japonica, Lespedeza cuneata, Spondiopogon cotuUfer. Cymbopogon tortilis, Plantago asiatica, Rumex acetosella, etc. The vegetation of Digitaria sanguinalis, Hydrocotyl japonica, Artemisia asiatica, etc. was com-paratively remarkable in the beginning.

• Journal of Ecology and Environment
• /
• v.44 no.1
• /
• pp.26-32
• /
• 2020

Background: Pollen is an important source of protein and lipids for many animals including honey bees. In order to understand the foraging behaviour of honey bee colonies and preference among the available floral resources, pollen collections from three experimental healthy colonies of honey bees were analysed in the month of June. Results: The amount of pollen collections were related to the colony's need which was indicated by the number of larval and adult bees present in the hive. Interesting was the sequence of pollen collection from different floral sources. All honey bee colonies collected pollens from Trifolium repens first, then Erigeron annus and the third choice was Coreopsis drummondii and Oenothera biennis flowers. Total protein content of Trifolium pollen was the highest (20.0 g/100 g DM), and the others were in the range of 8.9-11.4 g/100 g DM. Conclusion: The results indicated that the first criteria for honey bee foraging preference of pollens would be the nutritional contents of protein and the resource availability of the lesser nutritious floral sources. This information can help pollinator protection programmes of habitat manipulation using flowering plants for nectar and pollen sources.

• Journal of Plant Biology
• /
• v.34 no.2
• /
• pp.129-136
• /
• 1991

Two cell types, tip cells and hyphal cells, were found at the front of Cuscuta australis endophyte growing into the stem parenchyma of the host plant, Trifolium repens. Each tip cell developed into an elongate, filamentous hypha. The cells of both types possessed a dense cytoplasm including abundant organelles and enlarged nuclei with the deeply lobed envelope. The unevenly thick walls were observed in certain tip cells. The wall penetrated through the middle lamellae of the host cells and engulfed the debris of broken host cells. Some front cells had the plasmalemma-wall invaginations, which increased the surface area and would facilitate material uptake from the host No plasmodesmata between the host and parasite cells were found; instead, an apoplastic continuity was established by fused cell walls at the interface of the two partners. The apoplast was thought to be the main route for water and nutrients transport.nsport.

• Korean Journal of Weed Science
• /
• v.32 no.2
• /
• pp.139-143
• /
• 2012

This experiment was carried out to investigate the growth responses of white clover (Trifolium repens L.) as affected by prohexadione-calcium foliar application for the growth restraint in the lawn yard, field and golf course etc. The leaf length and leaf weight of white clover were significantly retarded compared to the untreated plot with foliar application of prohexadione-calcium after mowing within 1 day, and the effect was continued up to 50 days. The leaf area of clover with prohexadione-calcium treatment was to be narrowed as compared to untreated plot. The SPAD value of the leaf colour was increased in the treated plots and was to be continued for 40 days more. The coverage rate of clover was decreased with prohexadione-calcium foliar application after mowing in the lawn ground.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.42 no.3
• /
• pp.195-200
• /
• 2022

Sulfur is an essential element in plants, including amino acids, vitamin synthesis, and acting as an antioxidant. However, the interaction between endogenous sulfur and proline synthesis has not been yet fully documented. White clover (Trifolium repens L.) is known as a species highly sensitive to sulfate supply. Therefore, this study aimed to elucidate the role of sulfur in regulating proline metabolism in relation to ammonia detoxification and hydrogen peroxide (H₂O₂) accumulation in white clover. The detached leaves of white clover were immersed in solution containing different concentration of sulfate (0, 10, 100, and 1000 mM MgSO₄). As MgSO₄ concentrations were increased, the concentration of H₂O₂ increased up to 2.5-fold compared to control, accompanied with H₂O₂ detection in leaves. Amino acid concentrations significantly increased only at higher levels (100 and 1000 mM MgSO₄). No significant difference was observed in protein concentration. Proline and ∆^1-pyrroline-5-carboxylate (P5C) concentrations slightly decreased at 10 and 100 mM MgSO₄ treatments, whereas it rapidly increased over 1.9-fold at 1000 mM MgSO₄ treatment. Ammonia concentrations gradually increased up to 8.6-fold. These results indicate that exogenous sulfur levels are closely related to H₂O₂ and ammonia synthesis but affect proline biosynthesis only at a higher level.

• The Korean Journal of Pesticide Science
• /
• v.4 no.3
• /
• pp.68-74
• /
• 2000

Penicillium oxalicum (PENOX) has shown the potential as a biocontrol agent(5CA) for control of a weed, clover(Trifolium repens L.) in grass plots. The bioherbicidal activity may be due to germinative and growth capacities and substrate availability of the agent over a range of environmental factors. The influences of different water activities($0.94{\sim}0.995\;a_w$) and temperatures($18{\sim}30^{\circ}C$) on mycelial growth, conidial germination, sporulation oil 2% MEA(malt extract agar) adjusted to different water activities with glycerol, and carbon source utilization using BIOLOG GN MicroPlate were determined in vitro. Decreases in $a_w$ on MEA caused a reduction in mycelial growth and conidial germination depending on temperature. The mycelial growth of PENOX was greatest at $30^{\circ}C/0.995\;a_w$. At some lowered water activity($0.97\;a_w$), the growth was similar between 25 and $30^{\circ}C$, and considerably decreased at lowered temperature($20^{\circ}C$). The germination rate was also greatest at $30^{\circ}C/0.995\;a_w$. Lag phase times for PENOX at $18^{\circ}C$ on MEA were >6hrs at tile whole $a_w$ level tested, and at 18 and $25^{\circ}C$ they were >18hrs and >12hrs at $0.94\;a_w$, respectively. However, its sporulation was some better at $0.97\;a_w$ than $0.995\;a_w$ or $0.94\;a_w$, and better at $20^{\circ}C$ than $30^{\circ}C$. In contrast, the number of carbon sources(niche size) utilized by PENOX varied with $a_w$ and temperature. Under some water stress condition($0.95\;a_w$), the agent utilized smaller number of carbon sources than $0.995\;a_w$ depending on temperature. The niche size at 0.995 and $0.95\;a_w$ were highest at $25^{\circ}C$, and showed 86 and 65, respectively. At $30^{\circ}C$, the niche size at 0.995 and $0.95\;a_w$ showed 84 and 50, respectively. There was no carbon source utilized by PENOX at $0.90\;a_w$ regardless of temperature. These information of tile fungal ecophysiology will be useful for the effective development of BCA.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.19 no.6
• /
• pp.123-132
• /
• 2016

The study was to introduce the revegetation measures for the improvement of the landscape in October, 2012 on the slope; two and four years after the introduction, the slope was examined to see the status of the revegetation. The result is as follows. The result from examining the soil on the slope, the soil was Loamy sand; which infers that the soil is in a poor condition for improving the scenery. Examining the characteristics of the vegetation-base materials, the soil acidity was pH 6.4 to 6.7; and the soil hardness was 21 to 24mm (hardness quotient of soil), this can be considered a favourable condition, which has no effect on the growth and development of plants. When it comes to the discovery of the species in the area, Lotus corniculatus var., Silene armeria L., Papaver rhoeas, Trifolium pratense L. developed and grew in spring after two years. And in the summer, Lotus corniculatus var., Dianthus chinensis L., Cosmos bipinnatus, Coreopsis drummondii L., Trifolium pratense were founded. And there were Lotus corniculatus var., Dianthus chinensis L., Aster yomena, Coreopsis tinctoria, Trifolium pratense L. in the autumn. According to the examination conducting after four years, Lotus corniculatus var., Dianthus chinensis L., Silene armeria, Cosmos bipinnatus, Coreopsis drummondii L., Papaver rhoeas, Trifolium pratense L. were founded in the spring, and Lotus corniculatus var., Dianthus chinensis L., Cosmos bipinnatus, Coreopsis drummondii L., Trifolium pratense grew in the summer. Lotus corniculatus var., Dianthus chinensis L., Astragalus sinicus, Aster yomena, Coreopsis tinctoria, Trifolium pratense L. were discovered in the autumn. Among the exotic species founded in the area, were Festuca arundinacea, Lolium perenne, Festuca rubra. When it comes to the protection of vegetation and dominance, Lotus corniculatus var., Metaplexis japonica, Coreopsis drummondii L. are the dominant species among the spring plants; and Lotus corniculatus var., Trifolium repens L., Erigeron annuus (L.) Pers. were the dominant plants in the summer. In the autumn, Lotus corniculatus var., Coreopsis drummondii L., Poa pratensis appear to be the dominant plants. The colonies of Pinus densiflora and Castanea crenata var. were formed around the South Sejong IC. Part of the area is occupied by the colony of Alnus hirsuta Turcz. ex Rupr. In terms of shrub, there were Indigofera pseudo-tinctoria, Robinia pseudoacacia, and Rosa polyantha var. genuina NAKA. As ground cover plants, Sasa borealis, Rubus crataegifolius Bunge were founded.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.18 no.3
• /
• pp.187-194
• /
• 1998

This study was conducted to investigate the effect of antagonistic bacteria and pathogenic fungi on growth and yields of white clover(Trifo1iurn repens L.) in continuous cropping soil(CCS) and non-continuous cropping soil(NCCS). The gowth experiment of white clover was conducted at pots in a vinyl house. White clover was established by seeding into pots of 12 cm in diameter and 9 cm in depth containing 1:l mixture of soil and vermiculite with antagonistic bacteria and pathogenic fungi. In dark culture experiment, white clover lived longer in treatment of antagonistic bacteria than in treatment of control, but lived shorter in CCS than in NCCS. Dry weight of white clover was increased by the inoculation of the antagonistic bacteria(p< 0.05), but decreased by the inoculation of the pathogenic fungi(p< 0.05) both CCS and NCCS. In conclusion, bacterization of white clover with antagonistic bacteria enhances the growth and yield of white clover.