• Journal of Life Science
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• v.15 no.2 s.69
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• pp.273-278
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• 2005

Trifolium pratense (red clover, Fabaceae) is a short-lived herbaceous species and the species is introduced from Europe or North America to Korea approximately 60 years ago. Allozyme variability was examined in populations representing this species. A high level of genetic variation was found in T. pratense populations. Ten of 19 loci $(52.6\%)$ showed detectable polymorphism. Genetic diversity was 0.220. The sexual reproduction, high fecundity, and colonization process are proposed as possible factors contributing to high genetic diversity. Genetic diversity (0.220) was lower than that (0.285) of North American red clover, T. pratense. Korean populations of red clover may be founded by a small sample of larger or moderate populations. An indirect estimate of the number of migrants per generation (Nm = 4.20) indicated that gene flow was extensive among Korean populations of this species.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.6
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• pp.123-132
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• 2016

The study was to introduce the revegetation measures for the improvement of the landscape in October, 2012 on the slope; two and four years after the introduction, the slope was examined to see the status of the revegetation. The result is as follows. The result from examining the soil on the slope, the soil was Loamy sand; which infers that the soil is in a poor condition for improving the scenery. Examining the characteristics of the vegetation-base materials, the soil acidity was pH 6.4 to 6.7; and the soil hardness was 21 to 24mm (hardness quotient of soil), this can be considered a favourable condition, which has no effect on the growth and development of plants. When it comes to the discovery of the species in the area, Lotus corniculatus var., Silene armeria L., Papaver rhoeas, Trifolium pratense L. developed and grew in spring after two years. And in the summer, Lotus corniculatus var., Dianthus chinensis L., Cosmos bipinnatus, Coreopsis drummondii L., Trifolium pratense were founded. And there were Lotus corniculatus var., Dianthus chinensis L., Aster yomena, Coreopsis tinctoria, Trifolium pratense L. in the autumn. According to the examination conducting after four years, Lotus corniculatus var., Dianthus chinensis L., Silene armeria, Cosmos bipinnatus, Coreopsis drummondii L., Papaver rhoeas, Trifolium pratense L. were founded in the spring, and Lotus corniculatus var., Dianthus chinensis L., Cosmos bipinnatus, Coreopsis drummondii L., Trifolium pratense grew in the summer. Lotus corniculatus var., Dianthus chinensis L., Astragalus sinicus, Aster yomena, Coreopsis tinctoria, Trifolium pratense L. were discovered in the autumn. Among the exotic species founded in the area, were Festuca arundinacea, Lolium perenne, Festuca rubra. When it comes to the protection of vegetation and dominance, Lotus corniculatus var., Metaplexis japonica, Coreopsis drummondii L. are the dominant species among the spring plants; and Lotus corniculatus var., Trifolium repens L., Erigeron annuus (L.) Pers. were the dominant plants in the summer. In the autumn, Lotus corniculatus var., Coreopsis drummondii L., Poa pratensis appear to be the dominant plants. The colonies of Pinus densiflora and Castanea crenata var. were formed around the South Sejong IC. Part of the area is occupied by the colony of Alnus hirsuta Turcz. ex Rupr. In terms of shrub, there were Indigofera pseudo-tinctoria, Robinia pseudoacacia, and Rosa polyantha var. genuina NAKA. As ground cover plants, Sasa borealis, Rubus crataegifolius Bunge were founded.

• International Journal of Oral Biology
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• v.44 no.3
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• pp.81-88
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• 2019

Trifolium pratense leaves (red clover) has been used in Oriental and European folk medicine for the treatment of whooping cough, asthma, and eczema, and is now being used to treat and alleviate the symptoms, such as hot flushes, cardiovascular health effects that occur in postmenopausal women. However, relatively little scientific data is available on the physiological activity of this plant. Therefore, in this study, we investigated the anti-cancer activity of T. pratense leaves using methanol extract of T. pratense leaves (MeTP) on human FaDu hypopharyngeal squamous carcinoma cells. MeTP inhibited the viability of FaDu cells by inducing apoptosis through the cleavage of procaspase-3, -7, and -9 and poly (adenosine diphosphate ribose-ribose) polymerase (PARP), downregulation of Bcl-2, and upregulation of Bax, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Live & dead assay, 4'6-diamidino-2-phenylindole stain, fluorescence-activated cell sorting analysis, and Western blot analysis. In addition, colony formation was slightly inhibited when FaDu cells were treated with a non-cytotoxic concentration (0.125 mg/mL) of MeTP and almost completely inhibited when cells were treated with 0.25 mg/mL MeTP. Collectively, these results indicate that MeTP induced cell apoptosis via caspase- and mitochondrial-dependent apoptotic pathways, and inhibited colony formation of cancer cells in FaDu human hypopharyngeal squamous carcinoma cells. These findings suggest MeTP should be considered for clinical development as a chemotherapeutic option in oral cancer.

• Proceedings of the Korean Society of Crop Science Conference
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• 1991.05a
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• pp.42-43
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• 1991

• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.1
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• pp.8-17
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• 1987

Four foreign pasture cultivars such as Lotus corniculatus cv. Maitland, Trifolium pratense cv. Deben, Lolium perenne cv. $S_{23}$ and .Dactylis glomerata cv. $S_{26}$ were tested with "Kwisan" series, a common upland soil in Southern part of Korea. The cultivars in single or mixture were grown in pot with two levels of light intensity, i.e. a full sun light condition and 50% interception. Dry matter yield of top and root and their seasonal changes, the number of tillers or branches per plant and finally competative effects of mixtures were evaluated by estimating the expected yield, relative yield totals and compensation index. The results are summarized as follows: 1. Maitland showed a significant decrease in dry matter yield in shade condition, but the number of branches of Maitland was higher than those of Deben. 2. Maitland showed higher dry matter yield than Deben during the mid-summer period, while $S_{23}$, showed higher dry matter yield than $S_{26}$ during early season. 3. Mixtures of $S_{23}$ and Maitland, and $S_{26}$ and Deben gave better response to grasses by increasing the number of tillers in shade condition. 4. Combinations of Lotus corniculatus cv. Maitland and Dactylis glomerata cv. $S_{26}$ and Trifolium pratense cv. Deben and Dactylis glomerata cv. $S_{26}$ gave higher relative yield totals and higher compensation index, which envisages that the pasture establishment rates can be enhanced when those mixtures were sown by the oversowing method in the upland soil where phosphate level is very low and infertile in general.n general.

• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.2
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• pp.97-102
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• 1987

To find out the nutrient contents of temperate grasses on the south and north slope, a field experiment was conducted with five forage species of Dactylis glomerata, Festuca arundinacea, Phleum pratense, Agrostis gigantea and Trifolium repens. It was treated by randomized block design with 4 replications and lasted from September, 1983 to October, 1986 in Suweon. The results obtained are summarized as follows: 1. Mean chemical compositions except NFE and crude fiber of the forage species on the south slope were a little more contented than that of north. But all crude components were significant difference among the species on the south and north exposure. 2. Production of TDN (total digestible nutrient), StE (starch equivalent) ME (metabolizable energy) and NEL (net energy lactation) of different grass species on the north slope were much more increased by 5-30% per ha (Dactylis glomerata by 5-876, Festuca arundinacea by 5%, Phleum pratense by 24-26% and Agrostis gigantea by 25-30%) than that of same grasses on the south, however, those of Trifolium repens were much more increased by 4 1-42% per ha in the south. 3. Mineral contents, Ca/P ratio and K/ (Ca + Mg) equivalent ratio of the species were slightly different between the exposures. But considering the factors of occuring grass tetany, those of the forage species on the north slope were better than that of same species on the south.

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.437-442
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• 2004

Forage legumes are being used as an important source of protein for dairy farm as well as nitrogen fertilizer. This experiment was conducted to evaluate forage production and quality of five legumes at Cheonan Yonam College Experimental Livestock Farm for 1 year(2001-2002). Five legumes were crimson clover (Trifolium incarnatum L.) 'Tibbee', hairy vetch(Vicia villosa Roth) 'Common', red clover(Trifolium pratense L.) 'Kenland', persian clover(Trifolium resupinatum L.) 'Leeton', alfalfa(Medicago sativa L.) 'Vernal'. All legumes were shown cold hardiness of 8 rating or higher except crimson clover(7 rating). Flowering stage was observed 23th of April for crimson clover, 13th of May for hairy vetch, 3rd of May for red clover, and 12th of May for alfalfa. But persian clover didn't at harvest. Dry rnatter(DM) of crimson clover(17.6%) was the highest among legumes. In DM and total digestible nutrients(TDN) yields, crimson clover and hairy vetch were higher than those of other legumes. The crude protein of hairy vetch(20.5%) was the highest among legumes. In neutral detergent fiber(NDF) and acid detergent fiber(ADF), persian clover was the lowest(35.0% and 25.0%). Calculations of relative feed value(RFV) based on NDF and ADF of legumes were over 125(Prime degree) except hairy vetch. RFV and TDN of persian clover were the highest among legumes. According to the results of this study, crimson clover and hairy vetch are excellent in forage yield. Persian clover is superior in forage quality among legumes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.61-70
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• 2008

The object of this experiment was to investigate the food habits of sika deer fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources; 5 species(Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass), legume sources; 5 species(Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources; 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources; 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea(Roxb.) Wight and Rumex crispus), and native grass sources; 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Five sika deer were selected which had nearly the same body weight(average 92.5kg) as experimental animal. Experimental roughages were prepared by 0.5kg(DM basis) per 1 species fed to sika deer, and the experiment was carried out from 08:00 to the next day 06:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. The sika deer ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species ranking by sika deer was observed like this: Quercus aliena Blume, Prunus jamasakura Sieb, Quercus serrata Thunb. and Trifolium pratense, respectively, and the lower intake species was Phragmites comunis Trin. On the other hand, compared to each roughage source, total intake amount by sika deer was showed as browse(36.5%), Legumes(34.2%), grasses(14.0%), native grasses(13.5%) and weeds(1.8%), respectively. Based on the result, the food habits of sika deer seems to be closer to typically browser type.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.127-132
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• 1999

No comprehensive study of yield and quality losses of forage legumes harvested and cured in spring has been conducted in Korea, therefore, this experiment was carried out to gain information on yield and quality, losses of alfalfa(Medicgo sativa L.), crimson clover (Trifolium incarnatum L.), and red clover(Trifolium pratense L.) during field curing in spring(mid-May). Alfalfa was highest in dry matter content of 15% at harvest, while crimson clover and red clover had 12.4 and 11.5%, respectively. Species differences for the dry matter content were maintained and consistent trends were observed during the field curing. Alfalfa took seven days to reach dry matter content over 80%, while both crimson clover and red clover took eight days. Crimson clover and alfalfa tended to show a higher leaf-stem ratio than red clover based on dry matter, but red clover showed a higher leaf-stem ratio than alfalfa and crimson clover based on crude protein content. Losses in dry matter of alfalfa from leaf shattering were higher than crimson clover and red clover, and similar trends were detected losses in crude protein for three different legumes. Fresh legume forages averaged higher in clude protein(CP), total digestible nutrients(TDN), and relative feed value(RFV), while dry legume forages averaged lower in acid detergent fiber(ADF) and neutral detergent fiber(NDF). Results of the experiment indicate that hay curing in spring affects crude protein concentration more than ADF and NDF, and this is due, in part, to leaf shattering caused by field operations.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.3
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• pp.199-207
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• 1996

This experiment was canied out to determine the effects of seeding method and seeding ratio in mixed cropping of forage rye and red clover on dry matter yield, nutritional yield and chemical characteristics of soil. The main treatment was two seeding methods(drilling and broadcasting) and the sub treatment was six seeding ratios between rye and red clover(T1; 150kg : 0 kg/ha, T2; 120 : 3, T3; 90 : 6, T4; 60 : 9, T5; 30 : 12, T6; 0 : 15). The experiment was performed at the College of Natural Science of Kon-Kuk University in Chungju in 1992. The results were summarized as follows; 1. Protein content was increased by increasing the seeding ratio of red clover, but content of NDF and ADF were decreased. 2. The seeding method did not affects significantly the dry matter yield, but broadcasting showed higher dry matter yield than drilling in the same seeding ratio. Especially, T3 of broadcasting was the highest yield of 13, 172 kg/ha The T3 of broadcasting and T4 of drilling showed hlghly crude protein yield of 1, 268 kgha and 1, 252 kg/ha, respectively. 3. Comparing the each seeding ratio of two seeding method, total nitrogen yield of shoot and root of drilling were higher than broadcasting. Especially, T2 and T4 of drilling were the highest in total nitrogen yield. 4. There were more increase in organic matter and nitrogen contents but decrease in K, Ca of soil by increasing the seeding ratio of red clover than soil before experiment. In the seeding methods, broadcasting was an effect of soil improvement.