• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.4
• /
• pp.965-972
• /
• 2005

To investigate the effects of Trichosanthes semen extract on the growth and apoptosis of human uterine cervical carcinoma cells. Effects of Trichosanthes semen extract on the growth of ME-180 cells were assayed by MTT assay. Apoptosis induced by Trichosanthes semen extract was detected by fluorescent microscopy, DNA fragmentation analysis and flow cytometry. Caspase-3 and caspase-8 activities were assayed. Trichosanthes semen extract induced ME-180 cells to die in a dose- and time-dependent manner. ME-180 cells treated with Trichosanthes semen extract exhibited typical characteristics of apoptosis. The population of Sub-G1 cells increased significantly, and the cells represented the reduced size, condensed chromatin and apoptotic bodies. They showed the decreased mitochondrial membrane potential and increased activities of caspase-3 and caspase-8. The results suggest that Trichosanthes semen extract induced ME-180 cell apoptosis and the activation of caspase and mitochondrial pathway were involved in the process of Trichosanthes semen extract-induced apoptosis.

• Korean Journal of Acupuncture
• /
• v.27 no.4
• /
• pp.15-23
• /
• 2010

Objectives : In order to confirm the anti-cancer effect of Trichosanthes kirilowii pharmacopuncture fluid, this study was proceeded. Methods : A549 lung cancer cells were cultured to be treated by Trichosanthes kirilowii pharmacopuncture fluid as dose dependent manner for 72 hours. And then the cell viability, nucleus fragmentaion, p21 and p53 protein expression, Bcl-2 and Bax protein expression, procaspase-3 PARP protein expression. Results : 1. Trichosanthes kirilowii pharmacopuncture fluid decrease A549 cell viability as dose dependent manner. 2. Trichosanthes kirilowii pharmacopuncture fluid induced the nucleus fragmentation in A549 lung cancer cells as dose dependent manner. 3. Trichosanthes kirilowii pharmacopuncture fluid increase the p21 and p53 protein expression. 4. Trichosanthes kirilowii pharmacopuncture fluid decrease the Bcl-2 protein expression but cannot affect the Bax protein expression. 5. Trichosanthes kirilowii pharmacopuncture fluid increase the activation of caspase-3 and PARP protein. Conclusions : As the above results, it was conclused the Trichosanthes kirilowii pharmacopuncture fluid had the anti-cancer effects to induce apoptosis.

• Korean Journal of Plant Taxonomy
• /
• v.50 no.3
• /
• pp.356-360
• /
• 2020

A new distribution of Trichosanthes cucumeroides (Ser.) Maxim. ex Franch. & Sav. is discovered in Korea. This species was collected from forest margins on Ando Island, Ando-ri, Nam-myeon, Yeosu-si, in Jeollanam-do. T. cucumeroides is clearly distinguished from other species of the genus by having three- or five-lobed leaf blades, linear-lanceolate and minute bracts with entire margins, and oblong to triangular-ovoid seeds. Here, we provide precise description, a taxonomic key to the Korean Trichosanthes species, illustrations, and photographs of its habitat. The Korean name for the species is established as 'Bul-geun-ha-neul-ta-ri' considering the reddish color of its fruit.

• Journal of Nutrition and Health
• /
• v.30 no.1
• /
• pp.25-31
• /
• 1997

The effects of Trichosanthes kirilowii Max. subfractions, which has long been used as a wild vegetable and folk medicine, on insulin activity and acute toxicity were investigated. Diabetes melitus was induced in male Sprague-Dawley rats by the injection of streptozotocin(STZ) into the tail vein at a dose of 45mg/kg. The hexane fraction of the plant was subfractioned by silica gel column chromatography and were administered orally for 14 days and the normal and STZ-control group were orally administered with tween 80. The body weight gain was monitored and plasma levels of glucose, cholesterol, triglyceride, free fatty acid HDL-cholesterol were determined. The plasma activities of aspartate aminotransferase(AST) and alanine aminotransferase (ALT) were analysed. The subjraction A of hexane fraction lowered plasma glucose levels significantly and increased insulin activity compared to that of STZ-control. The activities of AST and ALT were decreased by administration of subfractions A and C of hexane fraction. The intakes of the hexane fraction of Trichosanthes kirilowii Max. did not showed the acute toxicity. It is suggested from the results that the subfraction A of the hexane fraction of Trichosanthes kirilowii Max. showed a hypoglycemic effect on diabetic rats and that the subfraction may be beneficial for insulin secreation.

• Advances in Traditional Medicine
• /
• v.10 no.3
• /
• pp.222-230
• /
• 2010

Trichosanthes cucumerina Linn. (cucurbitaceae) is widely used in Indian folk medicine for variety of disease conditions. The aim of present study was to evaluate the antiulcer activity of 50% ethanolic extract of fruits of Trichosanthes cucumerina Linn. (TCFE) using various experimental models of gastric and duodenal ulceration in rats. Oral administration of 50% ethanolic extract of fruits of Trichosanthes cucumerina Linn. was evaluated in rats against ethanol, aspirin and pylorus ligated gastric ulcers as well as cysteamine-induced duodenal ulcers. In all the models studied, the antiulcer activity of TCFE compared with that of cimetidine (100 mg/kg, p.o.), an $H_2$ receptor antagonist. TCFE showed significant antiulcer activity in ethanol-induced and aspirin-induced gastric ulcer models. In 19 h pylorus ligated rats, significant reduction in ulcer index, total acidity and pepsin activity was observed with TCFE, when compared with the control group. Mucosal defensive factors such as pH, mucin activity and gastric wall mucous content was found to be increased with TCFE. TCFE was also, afforded remarkable protection in cysteamine-induced duodenal lesions. The antiulcer activity of TCFE was comparable with that of cimetidine. Thus, TCFE possess significant antiulcer activity against both gastric and duodenal ulcers in rats. The antiulcer activity may be attributed to its cytoprotective action and inhibition of acid secretary parameters.

• Natural Product Sciences
• /
• v.7 no.2
• /
• pp.27-32
• /
• 2001

In addition to known cucurbitacins, a glucosphingosine type cerebroside and amino acids were isolated from the roots of Trichosanthes kirilowii. The structure of cerebroside was determined as soya-cerebroside I by means of spectroscopic methods. Fifteen amino acids were identified as aspartic acid, glutamic acid, serine, glycine, histidine, citrulline, threonine, alanine, proline, tyrosine, valine, isoleucine, leucine, phenylalanine and tryptophan, among which the major components such as citrulline, phenylalanine, leucine/isoleucine and valine were isolated.

• Archives of Pharmacal Research
• /
• v.13 no.3
• /
• pp.285-288
• /
• 1990

The polysaccharide fraction from the rhizome of Trichosanthes kirilowii (Cu-curbitaceae) showed marked antitumor and cytotoxic activity with immunopotentiating activity. It was evidenced by the increase in the number of circulating leucocytes and peritoneal exudate cells and the recovery of lowered antibody forming activity in mice. The polysaccharide was mainly composed of glucose, galactose, fructose, manmose and xylose and a small amount of protein.

• YAKHAK HOEJI
• /
• v.39 no.5
• /
• pp.528-534
• /
• 1995

This investigation was aimed at the study of the antidiabetic activity and effect on hepatic glutathione metabolism of polysaccharide from Trichosanthes kirilowii in hyperglycemic rats with aboxan (175 mg/Kg, i.p.). As the results, the polysaccharide inhibited the increase of blood glucose, triglyceride level and lactate dehydrogenase activity, but cholesterol not changed. And it increased protein bound-SH, nonprotein bound-SH, glutathione level and inhibited the decrease of glutathione S-transferase.

• Korean Journal of Medicinal Crop Science
• /
• v.22 no.1
• /
• pp.53-59
• /
• 2014

This study was conducted to determine the effects of seed storage temperature and pre-treatment on seed germination, seedling quality and vigor index of wild Trichosanthes kirilowii and Trichosanthes kirilowii var. japonica. As a result the highest germination rate of T. kirilowii was the 95% at seed stored in room temperature and then soaking for 24 hours in $dH_2O$. And the highest germination rate of T. kirilowii var. japonica was 96% at seed stored in $2^{\circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). But the seed germination rate was non-significance in pre-treatment at seed stored in room temperature $2^{\circ}C$. Seedlings of T. kirilowii and T. kirilowii var. japonica showed not only the best seedling quality but also seedling vigor index in seed stored at $2^{\circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). In this research, T. kirilowii and T. kirilowii var. japonica seed were stored in room temperature or $2^{\circ}C$, and then sown in peat moss, seed germination rate was more than 90% and production of superior quality seedlings.

• Journal of Society of Preventive Korean Medicine
• /
• v.18 no.1
• /
• pp.83-92
• /
• 2014

Objective : This study was performed to investigate the antineoplastic effect of Astragalus membranaceus, Angelica gigas, Rhus verniciflua Stokes and Trichosanthes kirilowii on SNU-80 anaplastic thyroid carcinoma cell line. Method : We examined whether our herbal medicines decreases cell growth rate of SNU-80 using MTT assay. We performed western blot analysis to verify that our herbal medicines induces apoptosis via caspase-dependent mechanism. We also performed wound healing assay and transwell invasion assay to investigate whether our herbal medicines affects the migration and invasion of anaplastic cancer cells, SNU-80. We also carried out ELISA assay to know our herbal medicines suppresses the expression of proinvasive molecules, such as VEGF and MMP-2 secreted from SNU-80. Results : MTT assay demonstrates that Angelica gigas, Rhus verniciflua Stokes, and Trichosanthes kirilowii suppressed strongly the growth of SNU-80. Western blot analysis demonstrates that Trichosanthes kirilowii induces apoptosis activating the cleavages of caspases (caspase-8, caspase-3) and PARP. Wound healing assay demonstrates that Rhus verniciflua Stokes, and Trichosanthes kirilowii inhibited the migration of SNU-80. Transwell invasion assay demonstrates that Rhus verniciflua Stokes, and Trichosanthes kirilowii inhibited the invasion of SNU-80. Elisa assay demonstrates that Astragalus membranaceus, Angelica gigas, Rhus verniciflua Stokes, and Trichosanthes kirilowii suppressed the expression of VEGF and MMP-2. Conclusion : We could conclude that several herbal medicines suppresses the growth and inhibits the migration and invasion of SNU-80 which is anaplastic thyroid cancer cells. Especially, Rhus verniciflua Stokes, Trichosanthes kirilowii had stronger anti-cancer effect suggesting that we can apply them to treat anaplastic thyroid cancer.