• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.33-41
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• 1984

This study was conducted to examine the physiology of pine mushroom mycelia cultured with various media for artificial culture of pine mushroom. The results obtained were as follows: 1) Among the various media, the medium composed of honey, boiled pine mushroom and soil extract fluid, fibrous root extract fluid, dry yeast, $KH_2PO_4$ inositol, folic acid, and biotin was the best for the growth of pine mushroom mycelium. 2) The optimum temperature for germinating pine mushroom spore and for culturing pine mushroom mycelium, was $24^{\circ}C$ and the optimum pH was 4.5. 3) There was no significant difference in growth between the mycelium separated from the tissue of pine mushroom sporophore and that separated from the spore. 4) No noticeable effect was found on the growth if such salts as $ZnSO_4$, $MnSO_4$, $MgSO_4$, $CaCl_2$ and ferric citrate were added to the Hamada's medium. 5) The addition of fibrous root extract promoted the growth of pine mushroom mycelium. 6) As a carbon source of artificial media, honey was more effective than glucose. 7) The culture infiltration of Mortierlla growing often in Fairy Ring was good for the growth of mycelium compared with the control. 8) The addition of fibrous root extract, inositol, biotin, and folic acid to artificial culture media was greatly effective in growth. When the temperature was lowered $19^{\circ}C$ after mycelium has appeared, the formation of primordium was observed.

• The Korea Journal of Herbology
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• v.23 no.1
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• pp.1-8
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• 2008

Objectives : The purpose of this study was to investigate extracts from mixed culture of Oriental medicines and cereal medium and mycelia of Trichloma matsutake and Cordyceps militaris by fermentation to develop new material for pharmaceutical products and medicinal food, Methods : To evaluate physiological activities of OCM extracts, we examined antioxidant activity(total polyphenol contents, electronic donating ability, SOD-like activity), ${\beta}$-glucan contents, nitric oxide production and cytotoxicity by MTT assay. Results : Total polyphenol contents of fermented OCM(UF) and non-fermented OCM(UM) extracts were more than 40% UM and UF of DPPH radical scavenging activity was 25.67%, 23.43% respectively. Total polyphenol content of non-fermented extract (UME) was 12.57%, while that of fermented extract(UFE) was 7.05%. SOD like activity showed UM 85.35%, UF 76.18%, UME 58.42%, UFE 72.21%. UME, and UFE 31.43%, ${\beta}$-glucan contents of UME and UFE were more than 40%. NO productions of UME, and UFE showed a LPS dose dependent tendency. Cytotoxicity on Raw 264.7 cell showed more than 90% viability. Inhibitory effect of UFE on HT1080 cell growth was higher than UME. Conclusions : These results showed that extracts from mixed culture of mushroom mycelium and OCM have physiological activities which can be used in pharmaceutical products and medicinal food.

• The Korean Journal of Ecology
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• v.21 no.4
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• pp.389-399
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• 1998

this study was focused on the effects of fire on spatial change of vegetation landscape in rural region. Fire types recognized as erown fire, severe surface fire and light surface fire in order of increasing intensity were described in a fire map. GIS was introduced to understand the relationship between fire types and topographic conditions or vegetation types. We also investigated land-use type and regeneration strategies after burning. Fire intensity depended on topographic conditions and vegetation types. Special land-use type in this area was collection of edible mushroom (Tricholoma matsutake). Mushrooms had been obtained from Pinus densiflora forests existing as edaphic climax or managed artificially. Regeneration strategy in burned areas was to make sprouts from burned oak stumps. A higher density and growth rate of sprouts, as compared to those on unburned areas, facilitated vegetation succession from P. densiflora forest to oak forest and consequently led to change of landscape pattern.

• Mycobiology
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• v.43 no.3
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• pp.327-332
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• 2015

Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to be explored. To understand its expression in mushroom fungi, the PAL gene of the edible mushroom Flammulina velutipes (Fvpal) was cloned and characterized. The cloned Fvpal consists of 2,175 bp, coding for a polypeptide containing 724 amino acids and having 11 introns. The translated amino acid sequence of Fvpal shares a high identity (66%) with that of ectomycorrhizal fungus Tricholoma matsutake. Distinctively, the Fvpal expression in the mycelium was higher in minimal medium supplemented with L-tyrosine than with other aromatic amino acids. During cultivation of the mushroom on sawdust medium, Fvpal expression in the fruit body correspondingly increased as the mushroom grew. In the fruiting body, Fvpal was expressed more in the stipe than in the pileus. These results suggest that F. velutipes PAL activity differs in the different organs of the mushroom. Overall, this is first report to show that the PAL gene expression is associated with mushroom growth in fungi.

• The Korean Journal of Mycology
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• v.42 no.2
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• pp.150-158
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• 2014

Four ectomycorrhizal fungi were tested for their ability to utilize seven carbon sources in modified Melin-Norkrans medium. After 60 days of mycelial culture, the highest mycelial growth in Hygrophorus russula (KFRI 1987), Sarcodon aspratus, Leccinum extremiorientale (KFRI 1194), and Tricholoma matsutake (KFRI 1256) was observed with use of dextrin used as a carbon source. H. russula, S. aspratus (KFRI 1676), and L. extremiorientale showed the lowest mycelial growth on nutrient medium with pectin. The utilization of homoglycans (starch, dextrin) in seven strains (except for T. matsutake KFRI 1256) was higher than that of heteroglycan (pectin). The final pH values of all culture media were decreased by pH 1.1~3.0 compared with the initial pH values of culture media. The dominant color of mycelia was white and varied according to the carbon sources (yellow, brown, and purple) in some strains. A single colony was observed in L. extremiorientale cultured in liquid media containing four or five different types of carbon sources, whereas multiple colonies were formed in liquid media containing six different types of carbon sources by six strains.

• Journal of Korean Society of Forest Science
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• v.86 no.3
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• pp.399-404
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• 1997

This paper is presenting a practical result of environmental manipulation effect on pine mushroom Tricholoma matsutake yield and a discussion of key factor seeking for improving pine mushroom production by analyzing the effects on mushroom yield for 10 years with applying five kinds of environmental control at the pine stands located in Namwon, Chollabuk-do, Korea. The environmental controls included density control and forest floor manipulations, and the treatments were applied during early summer of 1983. The mushroom yield itself did not show statistically significant differences among the treatments. But, we could manifest the treatment effects by calculating the relative yield in percent on the basis of pretreatment yield collected in 1982. The forest floor manipulation with density control may affect pine mushroom yield in short term, and continuous management should be applied to keep and improve the mushroom production. The fine root activity was the most important factor of pine mushroom production at the Namwon research site since the floor raking resulted in the largest effect on the mushroom yield although the environmental condition for the growth of fungi is important for pine mushroom production. In addition, the pine mushroom forest with sandy soils demands adequate litter layer since the litter removal showed relatively detrimental effects on pine mushroom yield compared to that in litter covered plot at the research site. That is, soil texture should be considered for forest floor manipulation, and it is reconfirmed that the environmental control to improve pine mushroom production should be applied differently by each region.

• Journal of Korean Society of Forest Science
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• v.113 no.3
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• pp.282-291
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• 2024

In Korea, pine forests are continuously declining as they mature. Along with vegetation succession, mushroom succession also occurs. The productivity of pine mushroom, a mycorrhizal species that was introduced as a source of short-term forest income in the late 20th century, is also declining. Although the forest fires was originally blamed as the main cause of pine forests decline, it has not been identified as a significant factor in the decline of pine mushroom productivity. Pine mushrooms are mainly produced in pine-dominant stands ranging in age classes from 3 to 8, with the highest productivity occurring between 4 and 6 classes. Accordingly, the aging of pine forests and their succession to mixed forests were evaluated as the most important factors contributing to the decrease in pine mushroom productivity. Since tending practices in pine mushroom-producing forests contribute to maintaining and increasing the productivity of pine mushrooms in mature pine forests, production can be sustained through proactive forest/soil management. In recent years, attempts have been made to cultivate pine mushrooms. The method of producing a mycorrhizae-infected pine tree by planting a pine in front of a fairy ring of Tricholoma matsutake and then transplanting it to create a new pine mushroom-producing stand has been successful. However, the method may cause damage to the fairy ring and has difficulties to preserve the viability of mycorrhizal fungus during transplantation. Mycorrhizae-inoculated seedling production is proposed as an alternative technology, but difficulties remain in keeping the fungus in the seedling during transplantation. Research on the microbiome of the fairy ring of T. matsutake is being conducted to solve this problem. Bacteria and fungi that contribute to the mycelial growth of T. matsutake have been discovered. It is expected that the era of industrial cultivation of pine mushroom will soon be possible through a microbial ecological approach.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.3
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• pp.206-213
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• 2008

We observed the physical and chemical properties of a soil on pine mushroom picking areas where were located in the most upper and lower parts showing a comparative climatic characteristics in Korea. The slope gradients within the investigation areas which were divided into 100 quadrates of $1m^2$ ranged from $5.7{\sim}8.6{\beta}$ to $24{\sim}22.7{\beta}$ (left to right) and $4.5{\sim}6.8{\beta}$ to $13.5{\sim}17.8{\beta}$ (top to bottom) for Ponghwa and Gansung, respectively. The amount of clay and thickness of organic matter were significantly decreased with increasing slope gradient, resulting in decrease of the soil moisture content around a fairly ring-colony of Tricholoma matsutake which was observed under the relatively thicker organic matter layer beyond 3 cm depth. Soil pHswere weak acid and average EC was $0.44dS\;m^{-1}$ in both areas. The cations were in the order of Fe K > Na > Mg > Ca and Fe > K > Na > Ca > Mg for the upper(Gansung) and the lower (Ponghwa) part. And the amount of Fe was approximately $80dS\;m^{-1}$ or greater in the pine mushroom picking soil. From this, we could assume that the growth of the pine mushroom was closely related not only with iron but also soil moisture content.

• Korean Journal of Environment and Ecology
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• v.15 no.4
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• pp.379-393
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• 2002

Thirty-six plots (each size 100m2) have been set up and surveyed to investigate the plant community structure and the ecological density of Pinus densiflora for. eracta(Chunyang-type) community in Seobyeok-ri, Chungyang-myeon, bonghwa-gun, Kyeongsangbuk-do. Four communities, Community I (large DBH class), Community II (large DBH class), Community III (middle DBH class), Community IV(small DBH class), were classified into by mean DBH and mean height. Pinus densiflora for. eracta dominate in canopy layer, Fraxinus sieboldiana and Quercus mongolica dominate in understory layer, Rhododendron Schlippenbachii and R. mucronulatum dominate in shrub layer. It turned out that thefour communiteis had low species diversity(0.4320~0.9487; unit: 400$m^2$) and high similarity. By the result of ecological density analysis. the mean basal area was proportionated to mean DBH (cm) size. By the result of simple regression analysis between mean DBH(cm), mean distance(m), and the number of individual were as follow: Ecological distance(m) = 0.0934$\times$ DBH(cm) +0.6117, Number of individual=242.47$\times$ DBH(cm)$^{-1.009}$, Ecological distance=9.643$\times$No. of individua $l^{-0.7016}$. In addition to four communities were suitable to the growth of Tricholoma matsutake because average species were about 30~50 years old, litter layer was 0.5~2.5cm and the ratio of coverage shrub was 20% .