• The Korean Journal of Mycology
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• v.33 no.1
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• pp.30-34
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• 2005

Fifteen PGPF (plant growth promoting fungi) isolates were selected from 728 fungal isolates collected from rhizosphere of zoysiagrass in Korea. Identification of the 15 isolates was based on their morphological characteristics. They were classified as Gliocladium sp. (n=1), Penicillium sp. (n=5), Trichoderma sp. (n=3), Fusarium sp. (n=3), and unidentifed species (n=3). Of the 15 isolates, six (PF-31, PF-136, PF-238, PF-255, PF-395, PF-420) significantly promoted the growth of tomato seedlings, and three (PF-31, PF-101, PF-255) also promoted the growth of hot pepper and two (PF-31, PF-225) also promoted the growth of cucumber, The 15 PGPF isolates were divided into 4 groups based on root colonizing ability. Isolates PF-17, PF-101 and PF-225 were included in the group 1, which had high root colonizing ability.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.648-653
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• 2017

The aim of this study was to select various fungal strains indigenous to Korea that have the potential to produce cellulases, including filter paper activity (FPase), $endo-{\beta}$-1,4-glucanase (EG), and ${\beta}-glucosidase$ (BGL). Among the 25 species of Ascomycetes and the 32 species of Basidiomycetes tested in this study, the Bjerkandera adusta KUC10565, Heterobasidion orientale KUC10556, Hyphoderma praetermissum KUC10609, and Trichoderma harzianum KUC1716 all exhibited remarkably high FPase activity. In addition, the T. harzianum KUC1716 showed high levels of EG and BGL activity. This strain has been selected for further study because of their enzymatic potential.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.12
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• pp.1913-1922
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• 2018

Objective: To find out ways of improving fermentation quality of silage, the comparative analysis of fermentation characteristics and in vitro digestibility of tropical grasses silage applied with cellulases produced from Acremonium or Tricoderma species were studied in Thailand. Methods: Fresh and wilted Guinea grass and Napier grass silages were prepared with cellulases from Acremonium (AC) or Trichoderma (TC) at 0.0025%, 0.005%, and 0.01% on a fresh matter (FM), and their fermentation quality, chemical composition and in vitro digestibility were analyzed. Results: All silages of fresh Napier grass were good quality with lower pH, butyric acid, and ammonia nitrogen, but higher lactic acid content than wilted Napier grass and Guinea grass silage. Silages treated with AC 0.01% had the best result in terms of fermentation quality. They also had higher in vitro dry matter digestibility and in vitro organic matter digestibility at 6 and 48 h after incubation than other silages. Silages treated with lower levels at 0.005% or 0.0025% of AC and all levels of TC did not improve silage fermentation. Conclusion: The AC could improve silage fermentation and in vitro degradation of Guinea grass and Napier grass silages, and the suitable addition ration is 0.01% (73.5 U) of FM for tropical silage preparation.

• Journal of Mushroom
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• v.14 no.4
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• pp.127-135
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• 2016

Cellulose is the most abundant organic polymer constituent of the cell wall of green plants and of various forms of algae. The complexity of lignocellulosic biomass is a major challenge in industrial research. Most mushroom species that naturally grow on soil or wood possess cellulases and the corresponding enzymatic system and, potential candidates for the direct bioconversion of softwood polysaccharides into fermentable sugars. However, there have been fewer studies on mushroom cellulases than on fungi such as Trichoderma spp., exploit the full potential of mushroom cellulases. This review will focus on the current status ofmushroom cellulase research and applications and will provide insight into promising future prospects.

• Journal of Practical Agriculture & Fisheries Research
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• v.5 no.1
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• pp.8-12
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• 2003

The optimal log conditions of cultivation are as follows. 1) Log-length, 100~120cm 2) Hole-depth, 1~1.5cm 3) It takes 55~67 days for primodia induction is possible to harvest fruiting bodies for 6~7 months. Meanwhile, the total of 10 species pest including Trichoderma sp. occurred during the cultivation.

• Mycobiology
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• v.47 no.2
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• pp.230-241
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• 2019

The Great Sebkha of Oran is a closed depression located in northwestern of Algeria. Despite the ranking of this sebkha among the wetlands of global importance by Ramsar Convention in 2002, no studies on the fungal community in this area have been carried out. In our study, samples were collected from two different regions. The first region is characterized by halophilic vegetation and cereal crops and the second by a total absence of vegetation. The isolated strains were identified morphologically then by molecular analysis. The biotechnological interest of the strains was evaluated by testing their ability to grow at different concentration of NaCl and to produce extracellular enzymes (i.e., lipase, amylase, protease, and cellulase) on solid medium. The results showed that the soil of sebkha is alkaline, with the exception of the soil of cereal crops that is neutral, and extremely saline. In this work, the species Gymnoascus halophilus, Trichoderma gamsii, the two phytopathogenic fungi, Fusarium brachygibbosum and Penicillium allii, and the teleomorphic form of P. longicatenatum observed for the first time in this species, were isolated for the first time in Algeria. The halotolerance test revealed that the majority of the isolated are halotolerant. Wallemia sp. and two strains of G. halophilus are the only obligate halophilic strains. All strains are capable to secrete at least one of the four tested enzymes. The most interesting species presenting the highest enzymatic index were Aspergillus sp. strain A4, Chaetomium sp. strain H1, P. vinaceum, G. halophilus, Wallemia sp. and Ustilago cynodontis.

• The Plant Pathology Journal
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• v.26 no.1
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• pp.32-36
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• 2010

Twelve fungal isolates out of 123 isolates obtained from cysts and soils of potato cyst nematode (PCN)-infested fields in Central Java, Indonesia had parasitic abilities of over 50% on PCN eggs or females (cysts) in vitro pathogenicity tests. Cultural and morphological characters and DNA sequences of ribosomal genes in ITS region revealed that they were four isolates of Gliocladium (Trichoderma) virens, three isolates of Fusarium oxysporum, one of F. lateritium, one of Penicillium tritinum and two of Taralomyces spp. A hundred percent infections occurred in eggs or cysts by three fungal isolates G. virens, F. oxysporum and P. oxalicum, suggesting that these fungi may have a good potential for the PCN biocontrol. Especially, G. virens isolates, which occurred most frequently in the PCN-infested potato fields and are known to be highly adaptable to varying habitats, may be developed as reliable agents for controlling PCN with both egg- and cyst-parasitic capabilities and with high ecological adaptabilities.

• Journal of Mushroom
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• v.3 no.3
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• pp.115-118
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• 2005

This study was carried out to investigate the cultural charateristics of Fomitopsis pinicola in log woods. The optimal conditions for log cultivation as follows; 1) Light effect, natural light 2) $CO_2$, 0.2% below 3) Low temperature shock, $15^{\circ}C$ for 5 days 4) log-length, 100~120cm 5) It takes 52~65 days for primodia induction is possible to harvest fruiting bodies for 5~6 months. Meanwhile, the total of 10 species pest including Trichoderma sp. occurred during the cultivation.

• Mycobiology
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• v.41 no.4
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• pp.252-255
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• 2013

Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.

• Mycobiology
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• v.48 no.5
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• pp.392-398
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• 2020

This study was conducted to understand the dynamics of microbial communities of soil microorganisms, and their distribution and abundance in the indigenous microorganisms (IMOs) manipulated from humus collected from the forest near the crop field. The soil microorganisms originated from humus and artificially cultured microbial-based soil amendments were characterized by molecular and biochemical analyses. The bacterial population (2 × 10⁶~13 × 10⁶ CFU/g sample) was approximately 100-fold abundant than the fungal population (2 × 10⁴~8 × 10⁴ CFU/g sample). The 16S rDNA and ITS sequence analyses showed that the bacterial and fungal communities in humus and IMOs were mainly composed of Bacillus and Pseudomonas, and Trichoderma and Aspergillus species, respectively. Some of the bacterial isolates from the humus and IMOs showed strong inhibitory activity against soil-borne pathogenic fungi Fusarium oxysporum and Sclerotinia sclerotiorum. These bacteria also showed the siderophore production activity as well as phosphate solubilizing activity, which are requisite traits for biological control of plant pathogenic fungi. These results suggest that humus and IMOs could be a useful resource for sustainable agriculture.