• 한국어병학회지
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• 제24권3호
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• pp.297-305
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• 2011

A trap identification system for isolating functional sequences to allow the constitutive expression of foreign protein from Edwardsiella tarda was developed. Using the green fluorescent protein (GFP) reporter-based trap system, various functional sequences to drive heterologous expression of the GFP were selectable in Escherichia coli host. However from the bioinformatic sequence analysis, all the segments predicted as regulatory regions were not native promoters actually existing upstream of endogenous E. tarda genes. Instead, a number of non-authentic sequences, possibly resulted from the random shuffling and/or intermolecular ligation were also proven to be able to display a potent GFP expression in the recombinant E. coli. Further analysis with selected clones showed that both authentic and non-authentic sequences could function in as a constitutive promoter, leading quite a consistent and stable GFP expression after repetitive subcultures. Microscopic examination also confirmed the uniform pattern of GFP expression in every host bacterium. Semi-quantitative assay of GFP showed that there was no clear relationship between expression levels and organizational features of the promoters trapped. Functional promoter-like elements achieved in the present study could be a good starting material for multivalent genetic engineering of E. tarda in order to produce recombinant vaccines in a cost-effective fashion.

• Animal cells and systems
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• 제7권2호
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• pp.133-137
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• 2003

Secreted proteins and membrane proteins play key roles in the formation, differentiation, and maintenance of multicellular organisms. In this study, we undertook to characterize these protein types in the central nervous system of the marine snail Aplysia kurodai using a yeast-based signal sequence trap method. One hundred and three cDNA clones were obtained by screening 300,000 clones from the signal sequence trap cDNA library. Of these, twelve were identical to previously identified Aplysia genes, 19 were related to known proteins in other organisms, and 54 clones were novel. These 54 new genes had high signal peptide scores or were found likely to contain a transmembrane domain sequence. Only 18 of the 103 clones proved to be false positive. The study demonstrates that the signal sequence trap method is an effective tool for Isolating Aplysia genes encoding secreted and membrane proteins.

• 전력전자학회논문지
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• 제11권4호
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• pp.313-320
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• 2006

본 논문에서는 단상 NPC 멀티레벨 인버터의 출력단 고조파 저감을 위한 LC 트랩 필터의 설계 방법을 제시하였고, 출력전압 THD와 출력전류 고조파 FFT 분석을 수행하였다. 제시된 LC 트랩 필터는 일반적인 LCR 필터와 종속 접속된 구조를 가지며, 스위칭 주파수에 동조되었다. 인버터 시스템은 고전력 응용에 적합한 NPC 멀티레벨 인버터를 사용하였으며, 제어기는 DSP(TMS320C31)을 사용하여 구성하였다. 제안된 시스템의 효용성은 시뮬레이션과 실험 결과를 통하여 증명하였다.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2009년도 하계학술발표대회 논문집
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• pp.465-470
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• 2009

This study investigated the water saving technology for the toilet stool system in a water closet. Since in 21st century water would be the prospected factor to measure the wealth of a country instead of the oil, water saving technologies in the toilet stool in a closet system is getting more important than the cleaning stool and the reducing noise performances. According to the statistics in Korea the percentage of using water in toilet is about 27% to the total amount of water used. Therefore, this study theoretically approached the possible amount of water saving and suggested the new variable position straight trap device in the toilet stool system.

• Current Optics and Photonics
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• 제5권3호
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• pp.220-228
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• 2021

To improve the measurement accuracy of a solar-radiation observer instrument, aiming at the problem of multiorder-stray-light interference caused by the diffraction of the flat-field concave grating in the spectroscopic system, straylight suppression methods for different forms of optical traps are studied. According to the grating surface-scattering distribution-function model, the bidirectional scattering distribution function (BSDF) of a dust-polluted surface and the flat-field concave grating's transition area of the spectroscopic system is calculated, and a Lyot stop with blade baffle is designed to suppress this kind of stray light. For diffraction multiorder stray light, based on the theory of light-energy transmission, a design for precise positioning of the trench optical trap is proposed. The superiority of the method is verified through simulation and actual measurement. The simulation results show that in a spectroscopic system approximately 160 mm × 140 mm × 80 mm in size, the energy of the stray light is reduced by one order of magnitude by means of the trench optical trap and Lyot stop, and the number of beams is reduced from 5664 to 1040. The actual measurements show that the stray-light-suppression efficiency is about 69.4%, which is effective reduction of the amount of stray light.

• Journal of Electrical Engineering and Technology
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• 제9권2호
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• pp.635-642
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• 2014

The space charge behaviors of oil-paper insulation affect the stability and security of oil-filled converter transformers of traditional and new energies. This paper presents the results of the electrical aging of oil-impregnated paper under AC-DC combined voltages by the pulsed electro-acoustic technique. Data mining and feature extractions were performed on the influence of electrical aging on charge dynamics based on the experiment results in the first stage. Characteristic parameters such as total charge injection and apparent charge mobility were calculated. The influences of electrical aging on the trap energy distribution of an oil-paper insulation system were analyzed and discussed. Longer electrical aging time would increase the depth and energy density of charge trap, which decelerates the apparent charge mobility and increases the probability of hot electron formation. This mechanism would accelerate damage to the cellulose and the formation of discharge channels, enhance the acceleration of the electric field distortion, and shorten insulation lifetime under AC-DC combined voltages.

• 한국응용곤충학회지
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• 제58권2호
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• pp.143-149
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• 2019

본 연구는 황색점착트랩의 설치각도에 따른 해충의 유인특성을 구명하고자 실시하였다. 고설재배 딸기농장에서 황색점착트랩을 수직(단면), 수평 앞면 및 뒷면, 45도 앞면 및 뒷면 등 5개 각도처리로 설치하고 유인된 해충수를 조사하였다. 총채벌레류(꽃노랑총채벌레 우점)와 뿌리파리류(작은뿌리파리 우점)의 경우 45도 윗면, 수직, 수평 윗면은 45도 뒷면이나 수평 뒷면보다 많은 수가 유인되었다. 매미충류(괴테애매미충)는 수직 또는 45도 위면 트랩에서 통계적으로 많은 수가 유살되었고 전체적으로 45도 뒷면, 수평 윗면이나 뒷면은 적은 수가 유살되었다. 진딧물류(목화진딧물 우점)와 가루이류(딸기가루이 우점)는 발생밀도가 낮은 관계로 뚜렷한 경향이 나타나지 않았다.

• 수산해양기술연구
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• 제47권4호
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• pp.300-315
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• 2011

The investigation for the catch and species composition in the deep sea of the East Sea was done to search marine organisms using some kinds of traps with small commercial fishing vessel near Goseong, Korea from 2008 to 2009. The experiments was carried out with some different traps at the depth of 200m, 400m, 700m and 1,000m. In 2008, the amount of catch with rectangular, cone and drum net traps was 91.4kg with twelve trips and the proportion of catch with rectangular and cone traps was high. Dominant species caught were Aniwan whelk (57%), snow crab (12%), hunchback sculpin (7%) and red snow crab (5%). The catch of Aniwan whelk was done well with rectangular and cone traps at the depth of 700-1,000m. Snow crab was mainly caught with rectangular and cone traps at the shallower depth of 200m, while red snow crab caught a lot with cone trap at the deeper depth of 700m. In 2009, the amount of catch with rectangular, cone and round traps was 92.4kg with nine trips. The proportion of catch with round trap was higher than that with cone trap. Dominant species caught were snow crab (67.1%), Aniwan whelk (14.8%) and northern shrimp (9.8%). Snow crab was caught well with round trap at the depth less than 400m.

• 수산경영론집
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• 제50권1호
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• pp.1-15
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• 2019

Offshore trap fishery managers have payed in advance to attract excellent fishing crew in the minium grantee lay system. This practice can intensify competition among fishery management bodies and increase the burden of fishery manager about income tax and four social insurance programs. The purpose of this study is to identify the problems of advance payment practice in terms of fishery manager in offshore trap fishery. The main results of this study are as follows. First, under the TYPE 1 model, which is the current wage payment manner, there is a risk that fishery manager will not able to return the prepayment paid in advance when the income of fishery management bodies decrease. Second, the TYPE 2 or the TYPE 3 model which reduces or abolishes advance payment can alleviate the burden of fishery manager relative to the deduction of redemption amount, incentive to induce artificial expenses, income tax and four social insurance programs. In conclusion, advance payment practice in offshore trap fishery is placing a heavy burden on the fishery manager. In order to solve these problems in the future, we need to find solutions through similar case studies.

• 수산해양기술연구
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• 제60권2호
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• pp.161-169
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• 2024

This study employs Bayesian network analysis to quantitatively evaluate the risk of incidents in trap boats, utilizing accident compensation approval data spanning from 2018 to 2022. With a dataset comprising 1,635 incidents, the analysis reveals a mortality risk of approximately 0.011 across the entire trap boat. The study significantly identifies variations in incident risks contingent upon fishing area and fishing processes. Specifically, incidents are approximately 1.22 times more likely to occur in coastal compared to offshore, and the risk during fishing processes outweighs that during maintenance operations by a factor of approximately 23.20. Furthermore, a detailed examination of incident types reveals varying incidence rates. Trip/slip incidents, for instance, are approximately 1.36 times more prevalent than bump/hit incidents, 1.58 times more than stuck incidents, and a substantial 5.17 times more than fall incidents. The study concludes by providing inferred mortality risks for 16 distinct scenarios, incorporating fishing areas, processes, and incident types. This foundational data offers a tailored approach to risk mitigation, enabling proactive measures suited to specific circumstances and occurrence types in the trap boat industry.