• Journal of Microbiology and Biotechnology
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• v.34 no.4
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• pp.969-977
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• 2024

Indigo is a valuable, natural blue dye that has been used for centuries in the textile industry. The large-scale commercial production of indigo relies on its extraction from plants and chemical synthesis. Studies are being conducted to develop methods for environment-friendly and sustainable production of indigo using genetically engineered microbes. Here, to enhance the yield of bioindigo from an E. coli whole-cell system containing tryptophanase (TnaA) and flavin-containing monooxygenase (FMO), we evaluated tryptophan transporters to improve the transport of aromatic compounds, such as indole and tryptophan, which are not easily soluble and passable through cell walls. Among the three transporters, Mtr, AroP, and TnaB, AroP enhanced indigo production the most. The combination of each transporter with AroP was also evaluated, and the combination of AroP and TnaB showed the best performance compared to the single transporters and two transporters. Bioindigo production was then optimized by examining the culture medium, temperature, isopropyl β-D-1-thiogalactopyranoside concentration, shaking speed (rpm), and pH. The novel strain containing aroP and tnaB plasmid with tnaA and FMO produced 8.77 mM (2.3 g/l) of bioindigo after 66 h of culture. The produced bioindigo was further recovered using a simple method and used as a watercolor dye, showing good mixing with other colors and color retention for a relatively long time. This study presents an effective strategy for enhancing indigo production using a combination of transporters.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.2
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• pp.135-144
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• 1995

Understanding on nutrient solute movement during the course of freezing and thawing was attempted through laboratory and field obsevations. Small sectioned tubes with 5cm inner diameter, 0.2cm thick and 1cm long were connected to 30cm long soil columns for laboratory study. The columns were filled with soil, and treated with 20mmol/kg $KNO_3$ for upper 5cm. The upper end was set in the freezing section, and the lower end was set in the refrigerating section of a refrigerator. Temperature was controlled at $-7({\pm}1)^{\circ}C$ and $1.5({\pm}1)^{\circ}C$, respectively. After top 5cm soil was frozen, the columns were sectioned, and analyzed for $NO_3^-$, $NH_4^+$ and $K^+$. For field study, the 20cm inner diameter and lm long soil columns were installed in Chuncheon and Daegwanryung, where the altitude was 74m and 840m, respectively. The soils used were silt loam and clay loam. The top 20cm soils were treated with 50mmol/kg as $KNO_3$. The soil columns were taken during winter freezing and after thawing. By laboratiry study, upward movement of $NO_3^-$ and $K^+$ during the course of freezing was confirmed. The upward movement of $K^+$ was, however, one fifth to one tenth of $NO_3^-$. The upward movement of inorganic nitrogen as well as laboratory during the course of freezing, but large amount of nitrogen was lost from the profile after thawing in early spring. Leached nitrogen from the upper 20cm to lower part was 17 to 24 percents. The maximum depth of leaching during the experiment was 50cm for all soils. The net loss of inorganic nitrogen from the whole profile ranged 8.7 to 39.5 percents. The net loss was greater in Daegwanryung where temperature was lower and snowfall was larger than Chuncheon, and the loss was greater from the silt loam soil than clay loam soil of which percolation rate was small. The results implied that reasons for nitrogen loss during the winter might include surface washing by snow melt as well as leaching and denitrification.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.401-409
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• 2021

This study aimed to estimate the photosynthetic capacity of tomato plants grown in a semi-closed greenhouse using temperature response models of plant photosynthesis by calculating the ribulose 1,5-bisphosphate carboxylase/oxygenase maximum carboxylation rate (V_cmax), maximum electron transport rate (J_max), thermal breakdown (high-temperature inhibition), and leaf respiration to predict the optimal conditions of the CO₂-controlled greenhouse, for maximizing the photosynthetic rate. Gas exchange measurements for the A-C_i curve response to CO₂ level with different light intensities {PAR (Photosynthetically Active Radiation) 200µmol·m^-2·s^-1 to 1500µmol·m^-2·s^-1} and leaf temperatures (20℃ to 35℃) were conducted with a portable infrared gas analyzer system. Arrhenius function, net CO₂ assimilation (A_n), thermal breakdown, and daylight leaf respiration (R_d) were also calculated using the modeling equation. Estimated J_max, A_n, Arrhenius function value, and thermal breakdown decreased in response to increased leaf temperature (> 30℃), and the optimum leaf temperature for the estimated J_max was 30℃. The CO₂ saturation point of the fifth leaf from the apical region was reached at 600ppm for 200 and 400µmol·m^-2·s^-1 of PAR, at 800ppm for 600 and 800µmol·m^-2·s^-1 of PAR, at 1000ppm for 1000µmol of PAR, and at 1500ppm for 1200 and 1500µmol·m^-2·s^-1 of PAR levels. The results suggest that the optimal conditions of CO₂ concentration can be determined, using the photosynthetic model equation, to improve the photosynthetic rates of fruit vegetables grown in greenhouses.

• Ocean Science Journal
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• v.40 no.1
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• pp.25-44
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• 2005

Long-term, continuous, and real-time ocean monitoring has been undertaken in order to evaluate various oceanographic phenomena and processes in the East/Japan Sea. Recent technical advances combined with our concerted efforts have allowed us to establish a real-time monitoring system and to accumulate considerable knowledge on what has been taking place in water properties, current systems, and circulation in the East Sea. We have obtained information on volume transport across the Korea Strait through cable voltage measurements and continuous temperature and salinity profile data from ARGO floats placed throughout entire East Sea since 1997. These ARGO float data have been utilized to estimate deep current, inertial kinetic energy, and changes in water mass, especially in the northern East Sea. We have also developed the East Sea Real-time Ocean Buoy (ESROB) in coastal regions and made continual improvements till it has evolved into the most up-to-date and effective monitoring system as a result of remarkable technical progress in data communication systems. Atmospheric and oceanic measurements by ESROB have contributed to the recognition of coastal wind variability, current fluctuations, and internal waves near and off the eastern coast of Korea. Long-tenn current meter moorings have been in operation since 1996 between Ulleungdo and Dokdo to monitor the interbasin deep water exchanges between the Japanese and Ulleung Basins. In addition, remotely sensed satellite data could facilitate the investigation of atmospheric and oceanic surface conditions such as sea surface temperature (SST), sea surface height, near-surface winds, oceanic color, surface roughness, and so on. These satellite data revealed surface frontal structures with a fairly good spatial resolution, seasonal cycle of SST, atmospheric wind forcing, geostrophic current anomalies, and biogeochemical processes associated with physical forcing and processes. Since the East Sea has been recognized as a natural laboratory for global oceanic changes and a clue to abrupt climate change, we aim at constructing a 4-D continuous real-time monitoring system, over a decade at least, using the most advanced techniques to understand a variety of oceanic processes in the East Sea.

• Journal of Korea Water Resources Association
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• v.45 no.9
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• pp.887-900
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• 2012

Drought is a non-negligible disaster of nature and it is mainly caused by rainfall shortage for a long time though there are many definitions of drought. 'Standard Precipitation Index' (SPI) that is widely used to express the level of meteorological drought intensity has a limit of not being able to consider the hydrological changes such as rainfall and evapotranspiration caused by climate change, because it does not consider the temperature-related variables other than the precipitation. Recently, however, 'Standardized Precipitation Evapotranspiration Index' (SPEI), a drought index of new concept which is similar to SPI but can reflect the effect of temperature variability as well as the rainfall change caused by climate variation, was developed. In this study, the changes of drought occurrence in South Korea were analyzed by applying SPEI for meteorological data (1973~2011) of 60 climate observatories under Korea Meteorological Administration (KMA). As the result of application, both of SPI and SPEI showed the trend of deepening drought in spring and winter and mitigating drought in summer for the entire nation, with SPI showing greater drought intensity than SPI. Also, SPI and SPEI with 12 months of duration showed that severe droughts with low frequency of around 6 years are generally being repeated.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.9 no.1
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• pp.94-100
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• 1999

Mercurous chloride($Hg_2Cl_2$) crystals hold promise for many acousto-optic and opto-electronic applications, which are prepared in closed ampoules by the physical vapor transport(PVT) growth methods. The thermal boundary conditions established by imposing different temperature on sidewalls of the enclosure cause simultaneous horizontal and vertical convectie flow in the PVT processes of$Hg_2Cl_2$ . It is found that for the ratios of horizontal to vertical thermal Rayleigh numbers$Ra_H/Ra{\ge}1.5$, the convective flow structure changes from multicellular to unicellular for the base parametric state of Ra=($2.79{\times}10^4$) , Pr=0.91, Le=1.01, Pe=4.60, Ar=0.2 and$C_V =1.01$. For the $\Delta T^{*}_H$ greater than 0.3, the $$\mid$U$\mid$_{max}$is increased with increasing $\Delta$ T^{*}_H$ and decreasing the aspect ratio. For the aspect ratios ranging from 0.1 to 1.0, there is a direct and linear relationship between $$\mid$U$\mid$_{max}$ and $\sqrt{{\Delta}T^_H\;^{\ast}}$.A decrease in the aspect ratio destabilizes the convective flow and results in an increase of the magnitude of convection in the crystal growth reactor. The vertical gradient tends to destabilize the convective flow which leads to oscillations, whereas the horizontal gradient stabilizes the convection.

• Journal of the Korean Geotechnical Society
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• v.34 no.10
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• pp.29-38
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• 2018

The slime formed at the bottom of the borehole causes the excessive displacement and loss of the bearing capacity of the drilled shaft. In this study, the slime meter is developed for the evaluation of the slime based on the electrical properties of the fluid and the slime in the borehole. The slime meter is composed of a probe instrumented with electrodes and temperature sensor and a frame with rotary encoder, so that the slime meter profiles the electrical resistivity compensated with temperature effect along the depth. For the application of the slime meter, three field tests are conducted at a borehole with a diameter of 3 m and a depth of 46.9 m with different testing time and locations. For all the tests, the experimental results show that while electrical resistivities are constantly measured in the fluid, the electrical resistivities sharply increase at the surface of the slime. Therefore, the slime thicknesses are estimated by the differences in the depths of the slime surface and the ground excavation. The experimental results obtained at the same testing point with different testing time show that the estimated thickness of the slime increases by the elapsed time. Also, the estimated slime at the side of the borehole is thicker than that at the center of the borehole. As the slime meter estimates the slime in the borehole by measuring the electrical resistivity with simple equipment, the slime meter may be effectively used for the evaluation of the slime formed at the bottom of the borehole.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.06a
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• pp.113-114
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• 2009

Silicon carbide is one of the most attractive and promising wide band-gap semiconductor material with excellent physical properties and huge potential for electronic applications. Up to now, the most successful method for growth of large SiC crystals with high quality is the physical vapor transport (PVT) method [1, 2]. Since further reduction of defect densities in larger crystal are needed for the true implementation of SiC devices, many researchers are focusing to improve the quality of SiC single crystal through the process modifications for SiC bulk growth or new material implementations [3, 4]. It is well known that for getting high quality SiC crystal, source materials with high purity must be used in PVT method. Among various source materials in PVT method, a SiC powder is considered to take an important role because it would influence on crystal quality of SiC crystal as well as optimum temperature of single crystal growth, the growth rate and doping characteristics. In reality, the effect of powder on SiC crystal could definitely exhibit the complicated correlation. Therefore, the present research was focused to investigate the quality difference of SiC crystal grown by conventional PVT method with using various SiC powders. As shown in Fig. 1, we used three SiC powders with different particles size. The 6H-SiC crystals were grown by conventional PVT process and the SiC seeds and the high purity SiC source materials are placed on opposite side in a sealed graphite crucible which is surrounded by graphite insulation[5, 6]. The bulk SiC crystal was grown at $2300^{\circ}C$ of the growth temperature and 50mbar of an argon pressure. The axial thermal gradient across the SiC crystal during the growth is estimated in the range of $15\sim20^{\circ}C/cm$. The chemical etch in molten KOH maintained at $450^{\circ}C$ for 10 min was used for defect observation with a polarizing microscope in Nomarski mode. Electrical properties of bulk SiC materials were measured by Hall effect using van der Pauw geometry and a UV/VIS spectrophotometer. Fig. 2 shows optical photographs of SiC crystal ingot grown by PVT method and Table 1 shows electrical properties of SiC crystals. The electrical properties as well as crystal quality of SiC crystals were systematically investigated.

• 제어로봇시스템학회:학술대회논문집
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• 1991.10b
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• pp.1639-1645
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• 1991

Because of the important role LD converters play in the production of high quality steel, various dynamic models have been attempted in the past by many researchers not only to understand the complex chemical reactions that take place in the converter process but also to assist the converter operation itself using computers. And yet no single dynamic model was found to be completely satisfactory because of the complexity involved with the process. The process indeed involves dynamic energy and mass balances at high temperatures accompanied by complex chemical reactions and transport phenomena in the molten state. In the present study, a mathematical model describing the dynamic behavior of LD converter process has been developed. The dynamic model describes the time behavior of the temperature and the concentrations of chemical species in the hot metal bath and slag. The analysis was greatly facilitated by dividing the entire process into three zones according to the physical boundaries and reaction mechanisms. These three zones were hot metal (zone 1), slag (zone 2) and emulsion (zone 3) zones. The removal rate of Si, C, Mn and P and the rate of Fe oxidation in the hot metal bath, and the change of composition in the slag were obtained as functions of time, operating conditions and kinetic parameters. The temperature behavior in the metal bath and the slag was also obtained by considering the heat transfer between the mixing and the slag zones and the heat generated from chemical reactions involving oxygen blowing. To identify the unknown parameters in the equations and simulate the dynamic model, Hooke and Jeeves parttern search and Runge-Kutta integration algorithm were used. By testing and fitting the model with the data obtained from the operation of POSCO #2 steelmaking plant, the dynamic model was able to predict the characteristics of the main components in the LD converter. It was possible to predict the optimum CO gas recovery by computer simulation

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.48 no.5
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• pp.323-334
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• 2020

Since boundary layer transition has a significant impact on the aero-thermodynamic performance of hypersonic flight vehicles, capability of accurate prediction of transition location is essential for design and performance analysis. In this study, γ-Re_θt model is improved to predict transition of hypersonic boundary layers and validated. A coefficient in the production term of the intermittency transport equation that affects the transition onset location is constructed and applied as a function of Mach number, wall temperature, and freestream stagnation temperature based on the similarity numerical solution of compressible boundary layer. To take into account a Mach number dependency of transition onset momentum thickness Reynolds number and transition length, additional correlation equations are determined as function of Mach number and applied to Re_θc and F_length correlations of the baseline model. The suggested model is implemented to a commercial CFD code in consideration of practical use. Analysis of hypersonic flat plate and circular cone boundary layers is carried out by using the model for validation purpose. An improvement of prediction capability with respect to variation of Mach number and unit Reynolds number is identified from the comparison with experimental data.